Article
Biology
Srinivas Cheenu Kappadath, Benjamin P. Lopez
Summary: In this study, an ablative dosimetry model was developed to calculate the dose distribution for tumors and at-risk margins, based on numerical mm-scale dose modeling and clinical evidence. The necessary dose metrics needed to achieve complete pathologic necrosis (CPN) following Y-90 radioembolization were calculated for a range of tumor sizes and tumor-to-normal-liver uptake ratios.
Article
Chemistry, Multidisciplinary
Bineet Sharma, Yutao Ma, Harrison L. Hiraki, Brendon M. Baker, Andrew L. Ferguson, Allen P. Liu
Summary: Giant peptide vesicles were generated easily and robustly using an emulsion transfer method, able to withstand chemical and physical stresses while hosting cell-free expression reactions. Additionally, another cell-free expressed elastin-like polypeptide was successfully incorporated into the existing membrane of the peptide vesicles.
CHEMICAL COMMUNICATIONS
(2021)
Review
Biochemistry & Molecular Biology
Ridhima Goel, Deepak Gulwani, Priyanka Upadhyay, Vijaya Sarangthem, Thoudam Debraj Singh
Summary: The 3D cell culture technique has gained appreciation recently as a research model, showcasing rapid growth and diverse scaffold testing, especially in spheroid cultures. Spheroid cultures provide opportunities for diverse biological studies, such as drug discovery, metabolic profiling, stem cell research, and tumor biology. The existing spheroid fabrication techniques have limitations, and there is a need for an economical and bio-compatible substrate for high throughput spheroid production.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Sonja Dragojevic, Lindsay Turner, Drazen Raucher
Summary: In this study, a macromolecular drug delivery system was examined for its ability to specifically deliver doxorubicin to cancer cells. The cytotoxicity and mechanism of action of two doxorubicin derivatives were compared in dox-responsive and dox-resistant cell lines.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
Zhuoyue Chen, Qiang Zhang, Hongmin Li, Qi Wei, Xin Zhao, Fulin Chen
Summary: By modifying silk fibroin (SF) scaffolds with engineered elastin-like polypeptides (ELP), cell adhesion and proliferation can be improved, leading to enhanced formation of mature bone and cartilage tissues.
BIOACTIVE MATERIALS
(2021)
Review
Pharmacology & Pharmacy
Like Gong, Zhaoying Yang, Fan Zhang, Weiping Gao
Summary: Conjugation of cytokines with elastin-like polypeptides (ELPs) has shown great potential in addressing challenges such as poor stability, short circulation half-life, and difficulty in production. This approach offers extended circulation time, enhanced stability, increased soluble protein expression, simplified purification, improved drug delivery, and controlled release, with promising applications in the field.
ADVANCED DRUG DELIVERY REVIEWS
(2022)
Review
Biotechnology & Applied Microbiology
Yingshu Guo, Shiwei Liu, Dan Jing, Nianzu Liu, Xiliang Luo
Summary: Elastin-like polypeptides (ELPs) are thermally responsive biopolymers with unique properties that can be tailored through genetic engineering. They have been widely used in drug delivery and tissue repair applications due to their biocompatibility, prolonged drug release, and nanostructure formation capabilities.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Chemistry, Physical
Tom Lindeboom, Binwu Zhao, George Jackson, Carol K. Hall, Amparo Galindo
Summary: Water and elastin-like polypeptides undergo conformational changes and liquid-liquid phase separation at specific temperature, exhibiting unique fluid-phase behavior. At low pressure, closed-loop liquid-liquid equilibrium is observed, while liquid-liquid immiscibility bounded by two LCSTs is predicted under deep-sea conditions.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2021)
Article
Pharmacology & Pharmacy
Sonja Dragojevic, Lindsay Turner, Pallabi Pal, Amol Janorkar, Drazen Raucher
Summary: Glioblastoma (GBM) is a primary brain tumor with poor prognosis. Tumor recurrence and resistance to chemotherapy are the main challenges. This study evaluated the potential of using elastin-like polypeptide (ELP) hydrogels as a local drug delivery system for GBM treatment. Results showed that ELP hydrogels can be engineered to release anti-cancer drugs over time, significantly reducing GBM cell survival and proliferation in vitro. These findings highlight the potential of ELP hydrogels for improving prognosis in GBM patients after tumor resection.
Review
Biotechnology & Applied Microbiology
Yeongjin Noh, Eunjoo Son, Chaenyung Cha
Summary: With the development of soft robotics, there is an increasing demand for lightweight, mechanically compliant, stimuli-responsive, and programmable actuator systems. Smart polymeric materials that can change their physicomechanical properties in response to external stimuli are being explored. Elastin-like polypeptide (ELP), a genetically-engineered thermoresponsive polypeptide, has unique characteristics and is being investigated for stimuli-responsive actuation, especially in biomedical applications.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Pharmacology & Pharmacy
Aiguo Jiang, Xinqiang Guan, Lianping He, Xingang Guan
Summary: Drug delivery systems (DDSs) based on elastin-like polypeptides (ELPs) have gained attention for their potential in improving drug loading and delivery efficiency in cancer treatment. ELPs possess excellent biocompatibility, degradability, temperature-responsiveness, and controllable sequence and structure, making them suitable for various applications in tissue engineering and cancer therapy. ELPs-based DDSs can enhance the pharmacokinetics and biodistribution of therapeutic agents, leading to improved antitumor efficacy. This review focuses on the recent advancements in using ELPs for delivering functional peptides, therapeutic proteins, small molecule drugs, and photosensitizers in cancer treatment.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Vijaya Sarangthem, Harshita Sharma, Ridhima Goel, Sampa Ghose, Rang-Woon Park, Sujata Mohanty, Tapan Kumar Chaudhur, Amit Kumar Dinda, Thoudam Debraj Singh
Summary: Extracellular matrix molecules are important for regulating cell behavior and tissue repair. Researchers proposed the use of human elastin derived materials to mimic biological functions. Elastin-like polypeptides (ELPs) containing ECM domains were genetically synthesized to regulate cell behavior and mimic natural matrices. Fusion ELP derivatives promoted cell attachment, migration, and tube formation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Physiology
Gene L. Bidwell
Summary: ELPs are bioengineered proteins with a unique physical property of a thermally triggered inverse phase transition, which can be utilized for drug delivery. ELP-fusion proteins have various applications, such as soluble biologics, thermally targeted drug carriers, self-assembling nanoparticles, and slow-release drug depots, making them a promising platform for the development of biologics.
Article
Biophysics
Ning Liu, Meiying Cui, Nannan Hu, Fuxu Yang, Yeteng Mu, Chong Guo, Xingang Guan, Zhigang Xie
Summary: This study demonstrates the great potential of ELPs-based nanocarriers for targeted delivery of antitumor peptides or proteins in cancer therapy. The multifunctional nanoparticles can significantly suppress tumor growth and increase apoptotic cell death.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Biochemistry & Molecular Biology
Yusuke Sugioka, Jin Nakamura, Chikara Ohtsuki, Ayae Sugawara-Narutaki
Summary: This study reports the formation of thixotropic hydrogels containing nanofibers of double-hydrophobic elastin-like polypeptides at a concentration an order of magnitude lower than previously reported ELP hydrogels. Chemical crosslinking of nanofibers improves the recovery of the hydrogels, providing deeper insight into the structure-property relationships of self-assembling polypeptides.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Chemistry, Multidisciplinary
Zhiyuan Shi, Yong Hu, Xin Li
Summary: Controlled drug delivery systems that can respond to mechanical force offer a unique solution for on-demand drug activation and release. Among various mechanical stimuli, ultrasound (US) has advantages in achieving spatiotemporally controlled drug release. Traditional US-triggered drug release relies on heat-induced phase transitions or chemical transformations, while the cutting-edge approach of Sonopharmacology leverages polymer mechanochemistry. The remaining challenges and potential future directions in this field are also discussed.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Lijie Zheng, Yuanzheng Chen, Xun Gu, Yingying Li, Hanqing Zhao, Wenjun Shao, Tao Ma, Chuanbin Wu, Qingqing Wang
Summary: In this study, a novel dosage form consisting of dissolving microneedles and an adhesive transdermal patch was developed for the treatment of rheumatoid arthritis. In vitro and in vivo experiments demonstrated that the combination of drugs delivered by this dosage form effectively reduced joint inflammation and damage.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Kyungjik Yang, Hwa Seung Han, Seung Hwan An, Kyung Hoon Park, Keonwook Nam, Shinha Hwang, Yuyeon Lee, Sung Yeon Cho, Taehyung Kim, Deokyeong Choe, Sang Won Kim, Wonkyu Yu, Hyunah Lee, Jiyong Park, Sangguan You, Dong- Gyu Jo, Ki Young Choi, Young Hoon Roh, Jae Hyung Park
Summary: This study developed CP-loaded CS microcapsules to enhance the oral bioavailability of CP through controlled gastrointestinal delivery. The optimized microcapsules exhibited desirable physicochemical properties, showed anti-photoaging effects via antioxidant activity, and achieved controlled release in the gastrointestinal tract. This research provides a simple and economical approach for enhancing the oral bioavailability of CP for customized bioactive compound administration.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Huiyang Li, Shuo Liu, Wenjin Dai, Bingmei Yao, Yong Zhou, Sujia Si, Hairong Yu, Riguang Zhao, Fang Jin, Liqun Jiang
Summary: Changes in bodily fluid pressures are crucial in diseases like high-altitude pulmonary edema (HAPE). Researchers have developed hydrostatic pressure-sensitive multivesicular liposomes (PSMVLs) that can release drugs in response to pressure changes, with potential applications in HAPE treatment. Animal experiments showed that this system provides better protection for lung tissues and respiratory function, reducing the occurrence of pulmonary edema.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Qian Hu, Hongbing Lan, Yinmei Tian, Xiaonan Li, Mengmeng Wang, Jiao Zhang, Yulin Yu, Wei Chen, Li Kong, Yuanyuan Guo, Zhiping Zhang
Summary: Coacervate droplets formed through liquid-liquid phase separation have potential as delivery vesicles for therapeutics. However, their lack of physiological stability and membranes are challenges. In this study, polylysine-polynucleotide complex coacervate droplets with favorable stability were formulated to concentrate molecules and nanoparticles. Phospholipid membranes were further coated on the droplets to create coacervate-based artificial protocells (ArtPC) with membrane-like structures. These biofunctional ArtPC effectively reduced blood uric acid levels and prevented renal injuries.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Xiaowei Wang, Hongwei Lu, Fang Luo, Dan Wang, Apeng Wang, Xuelei Wang, Wenkai Feng, Xiaobo Wang, Jiayi Su, Mingliang Liu, Guimin Xia
Summary: Four novel lipid-like GEM diesters were synthesized and encapsulated into liposomes to improve the antitumor efficacy of Gemcitabine. The liposomes loaded with dimyristoyl GEM (LipodmGEM) showed enhanced cellular uptake, improved inhibition of cell migration, and a greatly extended half-life compared to free Gemcitabine. LipodmGEM successfully enriched the drug in the tumor and exhibited excellent anticancer efficacy in vivo with negligible systemic toxicity.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Pengyu Li, Jieyi Pan, Yating Dong, Yingying Sun, Yalong Wang, Kang Liao, Yili Chen, Xin Deng, Shihui Yu, Haiyan Hu
Summary: Chronic pulmonary infection caused by Pseudomonas aeruginosa is a serious public health problem with high mortality rates. In this study, infection-microenvironment responsive nanoparticles were developed to eradicate biofilms and inhibit virulence. These nanoparticles showed promising results in treating chronic pulmonary infections.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Yajing Sun, Ze Lu, John A. Taylor, Jessie L. S. Au
Summary: A recent development in cancer chemotherapy is the use of cytotoxics to induce tumor-specific immune response through immunogenic cell death (ICD). This study describes a method that utilizes immunostaining and machine-learning to identify cells with ecto-CRT in intact 3-dimensional tissues. The method was successfully applied to study drug-induced ICD in human bladder cancer.
JOURNAL OF CONTROLLED RELEASE
(2024)
Review
Chemistry, Multidisciplinary
Rafat Ali, Shantanu Sen, Rohil Hameed, Aamir Nazir, Sandeep Verma
Summary: This review provides a focused overview of emerging strategies for delivering gasotransmitters in a controlled and sustained manner to reestablish neurophysiological homeostasis.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Jing Chen, Xinyi Zhang, Jinshen Zhang, Zhaoxia Wang, Guilan Zhu, Ming Geng, Jinmiao Zhu, Yajun Chen, Wei Wang, Youcui Xu
Summary: In this study, a multifunctional responsive hydrogel system was developed for synergistic reoxygenation and chemo/photothermal therapy. The hydrogel system showed both therapeutic effects against metastatic breast cancer and wound infection, making it a promising strategy for treating and preventing tumor recurrence and associated wound infection.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Baoshan Huang, Na Zhang, Xinying Qiu, Rui Zeng, Shuimiao Wang, Mengxia Hua, Qing Li, Kaihui Nan, Sen Lin
Summary: This study revealed that robust ROS can oxidize mitochondrial DNA (ox-mtDNA) and cause its release into the cytosol, resulting in the activation of NLRP3 inflammasome. By using the mitochondria-targeted antioxidant SkQ1 and a novel mitochondria-targeted nanoparticle (SkQ1 NP), it was found that mitochondrial ROS scavenging could in situ inhibit DED-induced mtDNA oxidation and suppress NLRP3-mediated inflammation.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Wenqi Liu, Cheng Hu, Linyu Long, Shuyi He, Wen Zhang, Zhicun Wang, Li Yang, Yunbing Wang
Summary: Myocardial infarction is the leading cause of cardiovascular mortality, and current treatment methods have limitations. This study developed a smart carrier that can release different therapeutic substances for different pathological processes, effectively improving cardiac function, promoting cardiac repair, and preventing ventricular remodeling.
JOURNAL OF CONTROLLED RELEASE
(2024)