Article
Engineering, Environmental
Shu-Ting Zhang, Tao Li, Shi-Kai Deng, Jim C. Spain, Ning-Yi Zhou
Summary: This study reveals a novel bacterial metabolic pathway for the degradation of 4-nitroanisole, initiated by a three-component cytochrome P450 system (pnaABC). This system exhibits broad substrate specificity and can catalyze the degradation of nitroanisole derivatives.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Peter D. Giang, Luke R. Churchman, Jeanette E. Stok, Rochelle M. Soo, James J. De Voss
Summary: A study found that Rhodococcus globerulus (R. globerulus) isolated from soil beneath Eucalyptus sp. can thrive on mono-terpenes such as 1,8-cineole, p-cymene and (R)- and (S)-limonene as its sole sources of carbon and energy. The research revealed a novel cytochrome P450 (CYP108N12) in R. globerulus which may be responsible for initiating the biodegradation of structurally similar monoterpene compounds. This finding suggests that CYP108N12 has potential as a biocatalyst with specific hydroxylation capabilities.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2022)
Article
Agriculture, Multidisciplinary
Xiang Ning Su, Xue Song Liu, Chuan Ying Li, Yu Ping Zhang
Summary: The rice cytochrome P450 gene, specifically CYP90D5, has been found to play a crucial role in the degradation of isoproturon and acetochlor in rice plants. Its overexpression leads to increased resistance to these herbicides and improved plant growth. Additionally, CYP90D5 reduces the accumulation of isoproturon and acetochlor in rice while the CRISPR-Cas9 lines show the opposite effects, confirming its function in pesticide detoxification and degradation.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Environmental Sciences
Xiaolei Wang, Hong Lu, Qiansheng Li, Yang Zhou, Jiti Zhou
Summary: This study investigated the response mechanism of Rhodococcus pyridinivorans GF3 to the model anthraquinone compound 1-aminoanthraquinone-2-sulfonate (ASA-2). The results showed that strain GF3 possessed a unique gene region encoding enzymes related to anthraquinone ring cleavage. This enabled the efficient degradation and detoxification of anthraquinone compounds. Additionally, the degradation of ASA-2 intermediate catechol led to enhanced tricarboxylic acid cycle and ATP generation, promoting the up-regulation of genes involved in resistance and maintaining cell homeostasis.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Biotechnology & Applied Microbiology
Hong-ming Liu, Meng Yuan, Ai-min Liu, Lei Ren, Guo-ping Zhu, Li-na Sun
Summary: This study identified Rhodococcus sp. B2 as capable of degrading the chloroacetamide herbicide pretilachlor and characterized a gene cluster (EthABD(B2)) essential for this degradation. The study also revealed a novel metabolite and demonstrated the role of a P450 family oxygenase in the degradation process. These findings provide valuable microbial resources for the remediation of chloroacetamide herbicides-contaminated sites.
MICROBIAL CELL FACTORIES
(2021)
Article
Biotechnology & Applied Microbiology
Le Xue, Yiquan Zhao, Ling Li, Xinran Rao, Xinjie Chen, Fuying Ma, Hongbo Yu, Shangxian Xie
Summary: This study elucidated the molecular mechanism of guaiacol metabolism by R. opacus PD630 and highlighted the importance of GcoA(R) and GcoB(R) in the utilization of guaiacol. The findings provide guidance for the design of efficient biocatalytic systems for lignin valorization.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Chemistry, Analytical
Joan Zapiter, Jeffrey R. Harmer, Michel Struwe, Axel Scheidig, Bernd Clement, Paul Bernhardt
Summary: In this study, mARC1 and mARC2 were successfully immobilised on glassy carbon electrodes, and the redox potentials of the enzymes were determined using electrochemical and spectroscopic measurements. The kinetic parameters and apparent Michaelis constants of benzamidoxime reduction were obtained. The pH dependence and stability of the enzyme electrode were also investigated, demonstrating good stability over a period of at least 9 days.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Joan Zapiter, Jeffrey R. Harmer, Michel Struwe, Axel Scheidig, Bernd Clement, Paul Bernhardt
Summary: In this study, human mitochondrial amidoxime reducing component 1 and 2 (mARC1 and mARC2) were immobilized on glassy carbon electrodes using the crosslinker glutaraldehyde. Voltammetry experiments were performed with the electron transfer mediator methyl viologen, and the redox potentials of the enzymes were determined. The apparent Michaelis constants obtained from the experiments were comparable to those reported in previous literature. The kinetic parameters for the reduction of benzamidoxime were determined from simulated cyclic voltammograms, and the stability of the enzyme electrode over time was also evaluated. The results demonstrate that this biosensor is effective in screening new pharmaceutically important substrates and mARC inhibitors.
ANALYTICAL CHEMISTRY
(2022)
Review
Agriculture, Dairy & Animal Science
M. J. Kuhn, V Mavangira, L. M. Sordillo
Summary: Dairy cattle are at a high risk of disease around calving due to compromised immunity and oxidative stress, influenced by the metabolism of fatty acids and fat-soluble vitamins. The importance of the cytochrome P450 (CYP450) enzyme family in cattle health is underappreciated, with potential interactions between fatty acids and fat-soluble vitamins needing further exploration. Understanding the role of CYP450 in immune function and oxidant status could help in reducing postpartum disease occurrence.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Engineering, Environmental
Toshio Mori, Haruka Ohno, Hirofumi Ichinose, Hirokazu Kawagishi, Hirofumi Hirai
Summary: In this study, it was found that the white-rot fungus P. chrysosporium degrades neonicotinoid insecticides through the activity of at least two different CYP isozymes.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Biotechnology & Applied Microbiology
Jingyan Zhang, Ying Sun, Yeji Wang, Xin Chen, Lu Xue, Jingjing Zhang, Xiangcheng Zhu, Yanwen Duan, Xiaohui Yan
Summary: Rubiginones are part of the angucycline family and have been shown to enhance the cytotoxicity of vincristine against drug-resistant cancer cell lines. Through genome mining of Streptomyces sp. CB02414, new rubiginones were discovered and a biosynthetic pathway was proposed based on the production profiles of wild-type and mutant strains. This research paves the way for further studies on cytochrome P450-catalyzed oxidation of angucyclines and the development of novel compounds through combinatorial biosynthesis strategies.
MICROBIAL CELL FACTORIES
(2021)
Article
Biochemistry & Molecular Biology
Yu Shi, Qiong Qu, Chenyang Wang, Yingshi He, Yihua Yang, Yidong Wu
Summary: Insect CYP2 and mitochondrial clan P450 genes are found to have potential roles in chemical defense, in addition to their known involvement in biosynthesis or metabolism of endobiotics. Through in vitro experiments, it was observed that these genes in Helicoverpa armigera can catalyze the conversion of specific compounds into toxins, shedding light on the insect's chemical defense mechanisms. This study provides valuable insights into both insect chemical defense and the physiological functions of these genes in H. armigera and other insects.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Jia Tang, Yudai Matsuda
Summary: A new meroterpenoid biosynthetic gene cluster was discovered in Aspergillus insuetus CBS 107.25, where a terpene cyclase folds the precursor compound 1 into a new meroterpenoid species with a different conformation. Another cyclase in the strain also synthesizes a distinct cyclized product of 1, resulting in diverging of the biosynthetic pathway in the fungus.
Review
Toxicology
Slobodan P. Rendic, F. Peter Guengerich
Summary: This overview discusses the metabolic activation of various compounds by cytochrome P450 enzymes, with a focus on bioactivation reactions. The study identifies a higher level of activation for general chemicals, drugs, and natural products compared to physiological compounds. Enzymes such as P450 3A4, 1A2, 2E1, and 1A1 are highlighted for their roles in catalyzing the formation of activated metabolites from different compound groups.
ARCHIVES OF TOXICOLOGY
(2021)
Article
Toxicology
Si Chen, Xilin Li, Yuxi Li, Xiaobo He, Matthew Bryant, Xuan Qin, Feng Li, Ji-Eun Seo, Xiaoqing Guo, Nan Mei, Lei Guo
Summary: Lapatinib, a drug used for treating HER2-positive breast cancer, has been associated with hepatotoxicity. This study shows that lapatinib causes cytotoxicity in multiple types of hepatic cells and induces cell cycle disturbances, apoptosis, and DNA damage. The metabolism of lapatinib by cytochrome P450 (CYP) enzymes, particularly CYP3A5 and 3A7, plays a role in attenuating its cytotoxicity. Furthermore, one of the metabolites, N-dealkylated lapatinib, is more toxic than the parent drug and other metabolites.
TOXICOLOGICAL SCIENCES
(2023)
Review
Environmental Sciences
Bin Xu, Yinping Chen, Jie He, Shixiang Cao, Jiawei Liu, Rui Xue, Fengxue Xin, Xiujuan Qian, Jie Zhou, Weiliang Dong, Min Jiang
Summary: This article reviews the biodegradation mechanism of polylactic acid (PLA), focusing on microbial and enzymatic degradation, as well as key factors affecting degradation efficiency. Additionally, upcycling is proposed as a more recyclable strategy for waste plastic disposal.
ENVIRONMENTAL REVIEWS
(2022)
Article
Biotechnology & Applied Microbiology
Haojie Liu, Aiyong He, Shun Li, Lijie Xu, Ning Xu, Shixun Liu, Jie Zhou, Xiujuan Qian, Weiliang Dong, Min Jiang
Summary: This study comprehensively evaluated and optimized the production of acid-type sophorolipids (SL) by yeast Candida bombicola ATCC 22214. It achieved high yield and purity of acid-type SL, and compared the produced SL mixture with commercial products. The results demonstrated the yeast's capability to effectively produce acid-type SL with good activity and stability, providing a promising approach for the commercial production of acid-type SL.
BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR
(2022)
Article
Engineering, Chemical
Xinhai Zhou, Dawei Zhou, Xinhui Bao, Yang Zhang, Jie Zhou, Fengxue Xin, Wenming Zhang, Xiujuan Qian, Weiliang Dong, Min Jiang, Katrin Ochsenreither
Summary: This study optimized the production of palmitoleic acid (POA) by regulating dissolved oxygen. Dynamic agitation was found to significantly enhance the production of POA-rich lipids. Under the optimal conditions of 1000 r.min(-1) agitation speed and 1 vvm aeration rate, no ethanol was detected during fermentation, and a biomass concentration of 44.80 g.L-1 with 13.43 g.L-1 of lipid and 2.93 g.L-1 of POA was achieved. Transcription analysis showed that the ethanol synthetic pathway was downregulated under high agitation conditions, while the expression of key enzymes responsible for lipid and POA accumulation was enhanced.
CHINESE JOURNAL OF CHEMICAL ENGINEERING
(2023)
Letter
Biochemical Research Methods
Anming Xu, Xiaoxiao Zhang, Shixiang Cao, Xiaoli Zhou, Ziyi Yu, Xiujuan Qian, Jie Zhou, Weiliang Dong, Min Jiang
Summary: In this study, a novel transcription-associated fluorescence-activated droplet sorting method was developed for specific targeting of di-RL hyperproducers. Using this method, ten higher di-RL producing microorganisms were screened out, which showed significantly increased di-RL yield compared to the model strain. This study provides a rapid, reliable screening method for di-RL production and offers a way to measure di-RL yield in populations of droplets containing different environmental colonies.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Anming Xu, Jiawei Liu, Shixiang Cao, Bin Xu, Chengzhi Guo, Ziyi Yu, Xiaoqiang Chen, Jie Zhou, Weiliang Dong, Min Jiang
Summary: The application of polyester-degrading microorganisms or enzymes is an eco-friendly alternative to chemical recycling. However, current screening methods are time-consuming and unable to distinguish polyesterases from other enzymes. This study presents a novel approach that combines a synthetic fluorescent polyurethane analogue probe and droplet-based microfluidics to efficiently screen polyurethane-degrading microorganisms.
MICROBIAL BIOTECHNOLOGY
(2023)
Article
Chemistry, Applied
Lu Yang, Rongrong Bai, Bin Xie, Nisha Zhuang, Ziyao Lv, Minjiao Chen, Weiliang Dong, Jie Zhou, Min Jiang
Summary: This paper presents an amperometric biosensor for glutamate monitoring in microbial fermentation processes. The biosensor utilizes an immobilization strategy that involves a chitin-binding domain to immobilize L-glutamate oxidase on a screen-printed Prussian blue nanocube microchip. The biosensor exhibits enhanced sensitivity, a linear range, and retains its activity after extended usage. It is capable of real-time monitoring and shows good agreement with high-performance liquid chromatography results.
Article
Agriculture, Multidisciplinary
Jiasheng Lu, Wankui Jiang, Weiliang Dong, Jie Zhou, Wenming Zhang, Yujia Jiang, Fengxue Xin, Min Jiang
Summary: In this study, a microbial consortium was designed to directly produce butyl butyrate from lignocellulose without the supplementation of exogenous precursors and lipase. The highest concentration of butyl butyrate achieved was 34.42 g/L. Moreover, it was found that Trichoderma asperellum could further enhance butyl butyrate production from microcrystalline cellulose in the three-strain microbial consortium.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Xuejun Cheng, Jie Huang, Ruoxu Wang, Yue Xu, Nan Wu, Jie Zhou, Xueyang Liu, Hong Wang, Hongyu Chen
Summary: We demonstrate the spontaneous formation of enclosed compartments with microporous shells and enriched biopolymers through the coprecipitation of silica and biopolymers. This synthetic method allows for mild reaction conditions and random mixing of reagents. The inclusion of divalent cation Ca2+ is crucial for co-precipitation and the subsequent formation of hollow compartments. The discovery of these enclosed compartments containing biopolymers opens new avenues for theorizing the host of early life forms.
Article
Chemistry, Analytical
Fan Wang, Yuanbo Wang, Jiawei Zhang, Shiyue Zheng, Bin Xie, Sheng Lu, Jie Zhou, Chongqing Wang, Fang Wang, Min Jiang, Xiaoqiang Chen
Summary: In this study, a esterase-activated chemiluminescent probe (CL-EA) was designed and synthesized for the selective detection of esterases. The probe successfully emitted a strong chemiluminescent signal in the presence of esterases, enabling the highly sensitive and specific detection of esterases in aqueous solutions.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Engineering, Environmental
Jiawei Liu, Qingyi Zeng, Huirui Lei, Kaiyuan Xin, Anming Xu, Ren Wei, Ding Li, Jie Zhou, Weiliang Dong, Min Jiang
Summary: In this study, 20 strains capable of decomposing polyurethane (PU) and other plastics were isolated, including 11 bacteria and 9 fungi. The best PU degrader was found to be Cladosporium sp. P7. Additionally, it was discovered that PBA-PU is depolymerized via ester and urethane bond hydrolysis, followed by intracellular metabolism and mineralization of the monomers to CO2 and H2O.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Engineering, Environmental
Xiaoli Zhou, Pinjing He, Wei Peng, Jie Zhou, Min Jiang, Hua Zhang, Weiliang Dong
Summary: The mass production, consumption, and disposal of plastics pose a significant threat to the environment and human life, but recycling plastic waste remains challenging. This study successfully converted mixed plastic waste into gas and solid products, without generating any liquid waste. The solid product consisted of tiny carbon spheres with a semi-crystalline graphitic structure, while the gas had a high methane content and heat value. Techno-economic and carbon footprint analyses showed that upgrading mixed plastic waste into carbon black and natural gas substitutes had a high internal rate of return, short payout period, and negative carbon emissions. The profitability of the project is mainly influenced by the product price and plant capacity. Compared to other methods, this approach demonstrated higher economic and environmental benefits.
RESOURCES CONSERVATION AND RECYCLING
(2023)
Article
Biotechnology & Applied Microbiology
Xiujuan Qian, Huirui Lei, Xinhai Zhou, Lili Zhang, Wenxing Cui, Jie Zhou, Fengxue Xin, Weiliang Dong, Min Jiang, Katrin Ochsenreither
Summary: By optimizing the fermentation process and performing molecular strain engineering, the researchers successfully expanded the palmitoleic acid (POA) production capacity of Scheffersomyces segobiensis DSM27193. They developed a dual fermentation strategy to eliminate ethanol and pyruvate accumulation during fermentation. Key genes influencing POA production were over-expressed and a new pathway was introduced to improve the yield of the precursor acetyl-CoA. The resulting cell factory SS-12 achieved a POA production of 7.3 g/L, an 11-fold increase compared to the wild type.
MICROBIAL BIOTECHNOLOGY
(2023)
Article
Microbiology
Xiujuan Qian, Kaiyuan Xin, Lili Zhang, Jie Zhou, Anming Xu, Weiliang Dong, Min Jiang
Summary: Biotransformation of plastics or their depolymerization monomers as raw materials offers a better solution for plastic waste disposal. This study improved the utilization capability of Pseudomonas putida KT2440 for 1,4-butanediol (BDO) through mutation and laboratory evolution, resulting in the isolation of an excellent mutant strain NB10. The metabolic mechanism behind BDO degradation was revealed, providing insights for up-cycling BDO to high-value chemicals.
MICROBIOLOGY SPECTRUM
(2023)
Editorial Material
Biochemistry & Molecular Biology
Anming Xu, Jie Zhou, Lars M. Blank, Min Jiang
Summary: Enzyme-based plastic degradation and valorization of plastic-derived monomers has been identified as a promising solution to address the plastic waste problem. This article summarizes the challenges in implementing enzymatic plastic degradation in industry and discusses strategies to overcome these obstacles in order to fully harness the potential of enzymatic plastic degradation for a sustainable plastic economy.
TRENDS IN MICROBIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Xue-Qiang Chu, Li-Wen Sun, Cheng Ma, Jia-Wei Chen, Yu-Lan Chen, Shao-Fei Ni, Ming-Quan Zhu, Jie Zhou, Mengtao Ma, Zhi-Liang Shen
Summary: A reaction of β-trifluoromethylated enones with phosphine oxides in water solution was developed for the preparation of highly functionalized gem-difluorodienes with excellent Z-selectivity. The reaction occurred through a tandem phospha-Brook rearrangement and defluorination sequence under transition-metal-free conditions, efficiently overcoming some competitive side reactions. The activation of the substrates and enhanced reaction selectivity were attributed to favorable H-bonding interactions at the water-organic phase boundary.
