Article
Food Science & Technology
Carolina Ibarguren, M. Virginia Guitian, Romina M. Lenz, Soria M. Cecilia, M. Carina Audisio
Summary: Listeria monocytogenes is a foodborne pathogen that can survive in harsh food processing environments. The use of bacteriocins as a natural alternative to control this pathogen has been hindered by the emergence of resistant strains. This study compares the properties of susceptible and resistant strains, including growth, antibiotic susceptibility, cell morphology, virulence factor expression, and biofilm formation. The results contribute to the understanding of bacteriocin-resistance development in L. monocytogenes and its implications for the use of these peptides as biopreservatives.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Food Science & Technology
Natalia Wiktorczyk-Kapischke, Ewa Walecka-Zacharska, Krzysztof Skowron, Agnieszka Kijewska, Zuzanna Bernaciak, Justyna Bauza-Kaszewska, Zuzanna Kraszewska, Eugenia Gospodarek-Komkowska
Summary: The study investigates persistent Listeria monocytogenes strains in the food processing environment and characterizes their ability to invade cells, form biofilms, and their resistance to selected disinfectants. The results show that persistent strains exhibit higher tolerance to disinfectants and initiate biofilm formation more rapidly.
Article
Food Science & Technology
Reda Riesute, Joana Salomskiene, Alvija Salaseviciene, Irena Macioniene
Summary: In this study, anolyte was evaluated as a potential disinfectant for inhibiting bacterial growth in meat. It was found that anolyte effectively reduced the counts of L. monocytogenes and S. Typhimurium in beef cuts and minced pork, and these bacteria could not be detected in the samples treated with anolyte after 29 days of incubation. The use of anolyte as a disinfectant showed promising results in reducing bacterial contamination in meat.
Article
Food Science & Technology
Xin Liu, Wenjie Chen, Zhixin Fang, Ying Yu, Jing Bi, Jing Wang, Qingli Dong, Hongzhi Zhang
Summary: This study identified four L. monocytogenes STs in two RTE food plants in Shanghai, China, and found that the biofilm formation ability and resistance to chlorine-containing disinfectants varied among different STs. ST5 and ST121 showed stronger biofilm formation ability and resistance, highlighting the need for stronger surveillance and control measures for these two STs in food processing plants.
Article
Food Science & Technology
Rene Koppel, Jasmin Schade, Martin Peier
Summary: Listeria in food poses a serious risk to consumer health, and reliable methods are needed for detection. While various PCR systems have been developed for identifying Listeria strains, they may not detect all known strains. This study introduces a novel multiplex real-time PCR method that can simultaneously detect multiple common and known Listeria strains.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2021)
Article
Food Science & Technology
Svetoslav Dimitrov Todorov, Wilhelm Heinrich Holzapfel, Luis Augusto Nero
Summary: The Pediococcus acidilactici strains isolated from Brazilian artisanal cheese showed high bacteriocin activity against Listeria monocytogenes and Enterococcus spp. The bacteriocins produced by these strains exhibited bactericidal mode of action against certain strains and displayed safety-related genes, although the presence of possible virulence factors and antibiotic resistance should be further investigated.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Microbiology
Verena Oswaldi, Stefanie Lueth, Janine Dzierzon, Diana Meemken, Stefan Schwarz, Andrea T. Fessler, Benjamin Felix, Susann Langforth
Summary: This study characterized Listeria spp. isolates from tonsils, intestinal content of pigs, and the slaughterhouse environment using next-generation sequencing. The findings revealed multiple sources of entry and a wide distribution of clonal complexes in pigs and the pork production chain. The study emphasized the potential risk of pigs as a source of human disease-causing isolates and highlighted the importance of efficient cleaning and disinfection procedures. Additionally, the study found multiple resistances in non-pathogenic L. innocua isolates from pig samples, which may pose a risk of spreading resistances to pathogenic species.
Article
Chemistry, Multidisciplinary
Nikolaos D. Andritsos, Spiros Paramithiotis, Marios Mataragas, Eleftherios H. Drosinos
Summary: This study aimed to assess dominance dynamics of L. monocytogenes serotypes in food, finding that serogroup 1/2 strains have a competitive advantage over serotype 4b strains during refrigerated storage.
APPLIED SCIENCES-BASEL
(2021)
Article
Microbiology
Inna L. Garmasheva, Ljubov T. Oleschenko
Summary: This study aimed to select bacteriocinogenic strains among Enterococcus strains isolated from Ukrainian traditional dairy products. Molasses and steep corn liquor were used as a low-cost media for screening. The results showed that 34 Enterococcus strains produced metabolites that inhibited the growth of indicator strains, and five strains were found to possess enterocin genes.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Food Science & Technology
Danielle Nader Furtado, Bernadette Dora Gombossy de Melo Franco, Svetoslav Dimitrov Todorov
Summary: Differentiated Lactococcus lactis bacteriocin producers' strains using repPCR technique. The stability of bacteriocin was found to be influenced by applied chemicals, pH, and temperature. The addition of bacteriocin completely inhibited the growth of Listeria monocytogenes ScottA, Lactobacillus sakei ATCC 15521, or Enterococcus faecium ATCC 19434 in the study.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Infectious Diseases
Pavel A. Andriyanov, Pavel A. Zhurilov, Elena A. Liskova, Tatyana I. Karpova, Elena V. Sokolova, Yulia K. Yushina, Elena V. Zaiko, Dagmara S. Bataeva, Olga L. Voronina, Ekaterina K. Psareva, Igor S. Tartakovsky, Denis V. Kolbasov, Svetlana A. Ermolaeva
Summary: Susceptibility testing of 117 L. monocytogenes strains to 23 antibiotics revealed a varied response, with most strains showing sensitivity to multiple antibiotics but some strains exhibiting resistance to specific drugs. There was a shift in the proportion of resistant strains over time, with different antibiotics showing unique patterns of resistance evolution.
Article
Microbiology
Fabrizia Guidi, Cinzia Lorenzetti, Gabriella Centorotola, Marina Torresi, Cesare Camma, Alexandra Chiaverini, Francesco Pomilio, Giuliana Blasi
Summary: In this study, 84 atypical Listeria monocytogenes strains were characterized and assigned to a new MLST type (ST2801, CC218). The new ST was found to be widespread and persistent in a meat producing plant. Cluster analysis identified genetically close clusters suggesting a common source. All strains showed determinants for heavy metals resistance, stress response, biofilm production, and multidrug efflux pumps. Some strains carried plasmids associated with cadmium resistance. All strains exhibited a virulence profile.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Food Science & Technology
V. Brusa, M. Prieto, C. A. Campos, S. Epszteyn, A. Cuesta, V. Renaud, G. Schembri, M. Vanzini, S. Michanie, G. Leotta, M. Signorini
Summary: The study showed that in Argentina, the risk of acquiring listeriosis from consuming fermented sausages and dry-cured pork shoulder is minimal, with factors such as the use of LAB in production, pH values, and water activity levels playing crucial roles.
Article
Food Science & Technology
Shaoying Gong, Chaoqin Jiao, Ling Guo
Summary: This study found that beetroot extract exhibited antibacterial activity against L. monocytogenes by reducing its intracellular levels and inducing apoptosis-like death. Additionally, it could act as a preservative in cooked meat products.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Food Science & Technology
Luis Patarata, Filipa Carvalho, Maria Joao Fraqueza
Summary: This study evaluated the effect of eliminating nitrite from the manufacturing of cured loins on consumer color evaluation and the behavior of pathogenic bacteria. The results showed that the use of nitrite resulted in a color considered adequate by most consumers, while the absence of nitrite led to a weaker color. The hedonic evaluation did not fully reflect the color evaluation. The use of nitrite did not affect the survival of pathogens, but the reduction in water activity was the primary determinant influencing their survival. Despite the weaker color, consumers still appreciate other sensory aspects beyond color.
Article
Biotechnology & Applied Microbiology
Victoria Lopez-Alonso, Sagrario Ortiz, Alfredo Corujo, Joaquin Martinez-Suarez
JOURNAL OF FOOD PROTECTION
(2020)
Article
Chemistry, Multidisciplinary
Lourenco Bonneville, Sagrario Ortiz, Vera Maia, Luisa Brito, Joaquin Martinez-Suarez
APPLIED SCIENCES-BASEL
(2020)
Article
Biochemistry & Molecular Biology
Adela Bernabeu-Zornoza, Raquel Coronel, Charlotte Palmer, Victoria Lopez-Alonso, Isabel Liste
Summary: The study found that Aβ 42 peptides at high concentrations induce apoptotic cell death and DNA damage, with Aβ 42 fibrils having the strongest effect; moreover, Aβ 42 oligomers greatly reduce cellular proliferation; both oligomers and fibrils promote gliogenesis and neurogenesis in human neural stem cells, with oligomers showing a more pronounced effect.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Lourenco Bonneville, Vera Maia, Ines Barroso, Joaquin Martinez-Suarez, Luisa Brito
Summary: This study aimed to investigate the impact of dual-species biofilms of Listeria monocytogenes with Lactobacillus plantarum on the anti-Listeria activity of a commercial disinfectant. The presence of Lactobacillus plantarum enhanced the efficacy of the disinfectant against Listeria monocytogenes in dual-species biofilms.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Genetics & Heredity
Ahmad Al Khleifat, Alfredo Iacoangeli, Joke J. F. A. van Vugt, Harry Bowles, Matthieu Moisse, Ramona A. J. Zwamborn, Rick A. A. van der Spek, Aleksey Shatunov, Johnathan Cooper-Knock, Simon Topp, Ross Byrne, Cinzia Gellera, Victoria Lopez, Ashley R. Jones, Sarah Opie-Martin, Atay Vural, Yolanda Campos, Wouter van Rheenen, Brendan Kenna, Kristel R. Van Eijk, Kevin Kenna, Markus Weber, Bradley Smith, Isabella Fogh, Vincenzo Silani, Karen E. Morrison, Richard Dobson, Michael A. van Es, Russell L. McLaughlin, Patrick Vourc'h, Adriano Chio, Philippe Corcia, Mamede de Carvalho, Marc Gotkine, Monica P. Panades, Jesus S. Mora, Pamela J. Shaw, John E. Landers, Jonathan D. Glass, Christopher E. Shaw, Nazli Basak, Orla Hardiman, Wim Robberecht, Philip Van Damme, Leonard H. van den Berg, Jan H. Veldink, Ammar Al-Chalabi
Summary: There is a strong genetic contribution to the risk of Amyotrophic lateral sclerosis (ALS), with heritability estimates of up to 60%. Variants in ALS genes, including repeat expansion in C9orf72, inversion in VCP, and insertion in ERBB4, have been identified as associated with ALS risk and specific disease phenotypes.
NPJ GENOMIC MEDICINE
(2022)
Article
Oncology
Ana Montero-Calle, Marta Gomez de Cedron, Adriana Quijada-Freire, Guillermo Solis-Fernandez, Victoria Lopez-Alonso, Isabel Espinosa-Salinas, Alberto Pelaez-Garcia, Maria Jesus Fernandez-Acenero, Ana Ramirez de Molina, Rodrigo Barderas
Summary: This study analyzed the metabolic and functional differences between colorectal cancer cells with different metastatic characteristics and identified metabolic targets associated with metastatic tropism in CRC patients. The study found that LDLR, CD36, FABP4, SCD, FASN, LPL, and APOA1 were metabolic markers associated with CRC metastasis to the liver or lung, and were also associated with the prognosis of CRC patients.
FRONTIERS IN ONCOLOGY
(2022)
Article
Cell Biology
Laura Garcia-Corzo, Isabel Calatayud-Baselga, Lucia Casares-Crespo, Carlos Mora-Martinez, Juan Julian Escribano-Saiz, Rafael Hortigueela, Andrea Asenjo-Martinez, Antonio Jordan-Pla, Stefano Ercoli, Nuria Flames, Victoria Lopez-Alonso, Marcal Vilar, Helena Mira
Summary: The study reveals the role of the transcription factor LEF1 in the gene regulation of quiescent NSPCs. It also highlights the significance of alternative splicing as a post-transcriptional regulatory mechanism in the transition from stem cell activation to quiescence.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Athanasia Varsaki, Sagrario Ortiz, Patricia Santorum, Pilar Lopez, Victoria Lopez-Alonso, Marta Hernandez, David Abad, Jorge Rodriguez-Grande, Alain A. Ocampo-Sosa, Joaquin Martinez-Suarez
Summary: This study investigated the origin and prevalence of Listeria monocytogenes in dairy cattle farms in the Cantabria region of Spain. Through isolation and genomic sequencing, a high incidence of L. monocytogenes, including subtypes involved in human listeriosis, was found in the cattle farm environment. This highlights the potential foodborne illness risk in dairy farms and the need for effective management.
Article
Immunology
Isabel Cortegano, Mercedes Rodriguez, Susana Hernangomez, Alejandro Arrabal, Carlos Garcia-Vao, Javier Rodriguez, Sandra Fernandez, Juncal Diaz, Belen de la Rosa, Beatriz Solis, Cristina Arribas, Felipe Garrido, Angel Zaballos, Sergio Roa, Victoria Lopez, Maria-Luisa Gaspar, Belen de Andres
Summary: Bronchiolitis in children is a significant disease with high morbidity and mortality rates. This study focused on outpatient bronchiolitis children and found elevated levels of pro-inflammatory and regulatory cytokines in their nasal lavage fluid samples. Additionally, children with mild symptoms and non-RSV respiratory viruses showed increased levels of certain cytokines. The NLF samples from bronchiolitis children also contained more immunoglobulins and specific cell types. These findings suggest the potential therapeutic implications of using NLF samples to analyze immune responses.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Raquel Coronel, Adela Bernabeu-Zornoza, Charlotte Palmer, Rosa Gonzalez-Sastre, Andreea Rosca, Patricia Mateos-Martinez, Victoria Lopez-Alonso, Isabel Liste
Summary: This study investigates the effects of amyloid precursor protein (APP) overexpression on human neural stem cells (hNSCs) and identifies differentially expressed genes related to neuronal and glial differentiation processes, as well as signaling pathways such as Notch, Wnt, PI3K-AKT, and JAK-STAT.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Raquel Coronel, Victoria Lopez-Alonso, Marta I. Gallego, Isabel Liste
Summary: This article investigates the biological role of APP in hNSCs cells, revealing its association with cell proliferation and cell fate specification.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Proceedings Paper
Engineering, Electrical & Electronic
V Ramos, P. Marina, M. Torres-Ruiz, J. Suarez, V Lopez, I Liste, M. de Alba
Summary: The deployment of 5G technology is expected to improve connectivity and introduce new real-time use cases, but concerns about health effects related to radiation exposure have been raised. This study aims to assess the effects of RF exposure at specific frequencies used in 5G and other technologies on zebrafish, focusing on factors such as mortality, hatching, movements, and development.
2021 PHOTONICS & ELECTROMAGNETICS RESEARCH SYMPOSIUM (PIERS 2021)
(2021)
Article
Clinical Neurology
Gijs H. P. Tazelaar, Steven Boeynaems, Mathias De Decker, Joke J. F. A. van Vugt, Lindy Kool, H. Stephan Goedee, Russell L. McLaughlin, William Sproviero, Alfredo Iacoangeli, Matthieu Moisse, Maarten Jacquemyn, Dirk Daelemans, Annelot M. Dekker, Rick A. van der Spek, Henk-Jan Westeneng, Kevin P. Kenna, Abdelilah Assialioui, Nica Da Silva, Monica Povedano, Jesus S. Mora Pardina, Orla Hardiman, Francois Salachas, Stephanie Millecamps, Patrick Vourc'h, Philippe Corcia, Philippe Couratier, Karen E. Morrison, Pamela J. Shaw, Christopher E. Shaw, R. Jeroen Pasterkamp, John E. Landers, Ludo Van den Bosch, Wim Robberecht, Ammar Al-Chalabi, Leonard H. van den Berg, Philip Van Damme, Jan H. Veldink, Michael A. van Es
BRAIN COMMUNICATIONS
(2020)
Review
Multidisciplinary Sciences
Andreea Rosca, Raquel Coronel, Miryam Moreno, Rosa Gonzalez, Andreea Oniga, Alberto Martin, Victoria Lopez, Maria del Carmen Gonzalez, Isabel Liste
