Article
Medical Laboratory Technology
Sofie K. M. van Zundert, Pieter H. Griffioen, Lenie van Rossem, Sten P. Willemsen, Yolanda B. de Rijke, Ron H. N. van Schaik, Regine P. M. Steegers-Theunissen, Mina Mirzaian
Summary: This study developed and validated a LC-MS/MS method to quantify five tryptophan metabolites and investigated their correlation with body mass index (BMI) in pregnant women. The results showed a negative correlation between BMI and the analytes TRP, 5-HTP, and 5-HIAA, and a positive correlation with KYN.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Food Science & Technology
Jensen Cherewyk, Taylor Grusie-Ogilvie, Barry Blakley, Ahmad Al-Dissi
Summary: This study validated a new method using ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) to quantify 12 epimers of ergot alkaloids in wheat. The method showed low LOD and LOQ values, allowing for sensitive detection of ergot epimers. The study also found differences between the R and S-epimers, which has rarely been documented in previous research.
Article
Biochemical Research Methods
Shuailong Chen, Xuan Li, Shijia Liu, Longshan Zhao, Wenyong Zhang, Zhili Xiong, Hemi Luan
Summary: A rapid, sensitive, and high-throughput isotope dilution liquid chromatography-negative ion electrospray tandem mass spectrometry (IDLC-nESI-MS/MS) method was developed for accurate quantification of multiple ceramides. This method demonstrated good linearity and recovery, and could be applied for studying ceramide biology and disease diagnosis.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Anna A. Lobas, Elizaveta M. Solovyeva, Erik Saparbaev, Mikhail Gorshkov, Oleg Boyarkin
Summary: A spectroscopic method combining UV photofragmentation and mass spectrometry has been developed for identification and quantification of isomeric biomolecules, with the use of pre-selected wavelengths to accelerate data acquisition and maintain high accuracy. The method has been successfully applied to quantify isomeric peptides and achieved high accuracy under different LC separation gradients.
Article
Chemistry, Analytical
Elodie Lamy, Lea Pilyser, Claire Paquet, Elodie Bouaziz-Amar, Stanislas Grassin-Delyle
Summary: A hydrophilic interaction liquid chromatography - mass spectrometry method was developed for the quantification of acetylcholine and choline in human cerebrospinal fluid, demonstrating high sensitivity and selectivity. The method showed excellent precision and accuracy, making it suitable for the analysis of cholinergic neurotransmission in human pathologies and drug effects study.
Article
Medical Laboratory Technology
Jordi Farre-Segura, Neus Fabregat-Cabello, Chiara Calaprice, Laurent Nyssen, Stephanie Peeters, Caroline Le Goff, Etienne Cavalier
Summary: A rapid and specific LC-MS/MS method for glucagon quantitation has been developed and validated, showing stability and accuracy in comparison to other immunoassay methods. This method could potentially establish a standardized approach for glucagon analysis in routine care settings.
CLINICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Alice M. S. Rodrigues, Philippe Lebaron, C. A. Downs, Didier Stien
Summary: This article presents an optimization method for quantifying organic ultraviolet filters in sand samples with diverse compositions. By using the standard additions method and optimizing the extraction solvent and detection conditions in high-performance liquid chromatography, the study improves the efficiency of detection.
JOURNAL OF SEPARATION SCIENCE
(2021)
Article
Chemistry, Analytical
Natalia Pudelko-Malik, Jerzy Wisniewski, Dominika Drulis-Fajdasz, Piotr Mlynarz
Summary: A liquid chromatography-mass spectrometry method was developed to quantitatively analyze the presence of a chemical compound in mouse tissues. The study found that the compound is not only present in the liver, heart, and skeletal muscle, but also in different regions of the brain.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Chemistry, Analytical
Natalia Pudelko-Malik, Jerzy Wisniewski, Dominika Drulis-Fajdasz, Piotr Mlynarz
Summary: A liquid chromatography-mass spectrometry method was developed and validated for the quantitative analysis of a chemical compound in mouse tissues, showing its presence in various brain regions.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Chemistry, Analytical
C. Bottinelli, R. Nicoli, F. Bevalot, N. Cartiser, C. Roger, K. Chikh, T. Kuuranne, L. Fanton, J. Guitton
Summary: The study aimed to develop the first validated method for analyzing human insulin and its synthetic analogues, primarily for plasma and post-mortem sera. Different extraction processes were evaluated for the lowest limit of detection for target analogues, and the limits of quantification were validated in plasma and post-mortem sera. Results showed that at the LOQ level, accuracy was between 70 and 130%, with precision lower than 30%.
Article
Biochemical Research Methods
Shengnan Ji, Xiaomeng Liu, Jing Ha, Lianfeng Ai, Zheng Li
Summary: A sensitive and simple LC-MS/MS method was developed for the determination of orelabrutinib in human plasma and cerebrospinal fluid. The method involved simple protein precipitation, efficient separations, and optimized mass spectrometric conditions to increase selectivity and sensitivity. The method was validated for accuracy, precision, selectivity, linearity, recovery, matrix effect, stability, and limits of quantification. It exhibited a lower limit of quantification of 0.50 ng/mL, with intraday and interday precision RSD both less than 15%, and a recovery rate of 85.7%-92.9%. The method was successfully applied for quantitation in clinical patients treated with orelabrutinib.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Biochemical Research Methods
Ming Li, Ralf D. Josephs, Adeline Daireaux, Tiphaine Choteau, Steven Westwood, Gustavo Martos, Robert I. Wielgosz, Hongmei Li
Summary: Oxytocin is a crucial peptide used for labor induction and postpartum bleeding control, but also shows potential as a biomarker for emotions and cancer diagnosis. Accurate purity characterization through various methods is essential, considering the unique analytical challenge posed by the presence of disulfide bonds in Oxytocin. Newly developed LC-hrMS method has been effective in identifying and quantifying structurally related impurities in synthetic OXT materials.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Binbin Wang, Xiaoxiao Feng, Sihan Liu, Feng Qiu, Xuran Lu, Zhaoxia Li
Summary: A simple and accurate HPLC-DAD method was established and validated for the quantitative analysis of seven bioactive compounds in KXP, including the first quantification of DTU and DTR. The study provided a useful method for analyzing the major bioactive compounds in KXP and improving its quality assessment research.
Article
Biochemical Research Methods
Johannes B. Mueller-Reif, Fynn M. Hansen, Lisa Schweizer, Peter Treit, Philipp E. Geyer, Matthias Mann
Summary: Reversed-phase HPLC is commonly used in MS-based proteomics for peptide separation, with particle-packed capillary columns being predominantly used in nanoflow HPLC systems. Despite capillary columns being a standard for many years, they are expensive and have short lifetimes, leading many laboratories to produce their own in-house packed columns.
MOLECULAR & CELLULAR PROTEOMICS
(2021)
Article
Biochemical Research Methods
Masaki Takiwaki, Fumio Nomura, Mamoru Satoh, Sachio Tsuchida, Kazuo Otake, Junko Takagi
Summary: This study aimed to develop an LC/MS/MS-based method for detecting human plasma AVP and compare it with the traditional RIA method. Although there was a weak overall correlation between the results obtained using the two different methods, disagreements were observed at low concentrations.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Biochemical Research Methods
Jorg Winkler, Gianvito Urgese, Elisa Ficarra, Knut Reinert
Summary: The study introduces a software tool called LaRA 2 that is significantly faster and more powerful compared to other similar software for accurate pairwise and multiple structural alignments of RNA sequences. LaRA 2 can handle arbitrary pseudoknots and helps researchers analyze large sets of RNA secondary structures in a relatively short time, deriving structural motifs for search in genomic databases.
BMC BIOINFORMATICS
(2022)
Article
Biochemistry & Molecular Biology
Constantin Bloechl, Christoph Holzner, Michela Luciano, Renate Bauer, Jutta Horejs-Hoeck, Ulrich Eckhard, Hans Brandstetter, Christian G. Huber
Summary: This study re-evaluates the proteolytic properties of Streptococcal pyrogenic exotoxin B (SpeB) and identifies potential host substrates involved in streptococcal infection. The research provides insights into the activation and specificity of SpeB and contributes to understanding its role in infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemical Research Methods
Di Wang, Tao Zhang, Katarina Madunic, Antonius A. de Waard, Constantin Blochl, Oleg A. Mayboroda, Marieke Griffioen, Robbert M. Spaapen, Christian G. Huber, Guinevere S. M. Lageveen-Kammeijer, Manfred Wuhrer
Summary: This study performed a detailed analysis of GSL glycans in 19 AML cell lines and revealed significant differences in GSL glycan profiles among different cell lines. The abundance of GSL glycans was found to be associated with the transcriptomic expression of specific transcription factors and glycosyltransferases, providing insights into the regulation of GSL expression.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Plant Sciences
Martina Althammer, Christof Regl, Klaus Herburger, Constantin Blochl, Elena Voglas, Christian G. Huber, Raimund Tenhaken
Summary: Galactose toxicity (Gal-Tox) is a common phenomenon in plants, but little is known about the mechanism behind it. This study investigated the role of galactose-containing metabolites like galactose-1-phosphate (Gal-1P) and UDP-Gal in Gal-Tox. Overexpressing USP from Pisum sativum helped reduce Gal-1P levels and alleviate Gal-Tox symptoms in roots. However, the overexpression lines became more sensitive to exogenous galactose. This could be due to the elevated levels of UDP-Gal in the roots.
Article
Biochemistry & Molecular Biology
Fausto Gueths Gomes, Andre Cronemberger Andrade, Martin Wolf, Sarah Hochmann, Linda Krisch, Nicole Maeding, Christof Regl, Rodolphe Poupardin, Patricia Ebner-Peking, Christian G. Huber, Nicole Meisner-Kober, Katharina Schallmoser, Dirk Strunk
Summary: This study isolated extracellular vesicles (EVs) from platelet lysate and characterized them using various techniques. The researchers found that TFF-EVs derived from platelets had better functionality in skin-organoid formation and T-cell proliferation inhibition compared to other isolation methods. Additionally, the study revealed that the protein corona formed on TFF-EVs improved their functionality.
Article
Chemistry, Analytical
Katharina Boettinger, Wolfgang Esser-Skala, Marius Segl, Christoph Herwig, Christian G. Huber
Summary: The study used a simple HPLC-MS workflow to analyze mAb product quality in real-time at different fermentation time points. The results revealed the presence of misassembled variants in the fermentation broth, alongside changes in mAb glycosylation profile over time, with galactosylated variants being the most abundant. The workflow proved to be robust for analyzing the complex matrix with low standard deviations.
ANALYTICA CHIMICA ACTA
(2022)
Article
Cell Biology
Martin Wolf, Rodolphe W. Poupardin, Patricia Ebner-Peking, Andre Cronemberger Andrade, Constantin Bloechl, Astrid Obermayer, Fausto Gueths Gomes, Balazs Vari, Nicole Maeding, Essi Eminger, Heide-Marie Binder, Anna M. Raninger, Sarah Hochmann, Gabriele Brachtl, Andreas Spittler, Thomas Heuser, Racheli Ofir, Christian G. Huber, Zami Aberman, Katharina Schallmoser, Hans-Dieter Volk, Dirk Strunk
Summary: Nanoparticles derived from therapy-grade human placental-expanded stromal cells are surrounded by a protein corona, which improves skin regeneration and angiogenesis and induces differential signaling in immune cells.
JOURNAL OF EXTRACELLULAR VESICLES
(2022)
Article
Biochemistry & Molecular Biology
Isabel J. Hoppe, Bernhard Prommegger, Andreas Uhl, Urs Lohrig, Christian G. Huber, Hans Brandstetter
Summary: This study introduces a fluorescence-based enzyme cascade method that can detect chemical and conformational changes in protein samples and establish databases of modification-function relationships for proteomic research.
Article
Chemistry, Medicinal
Alexandra Pohler, Sara Abdelfatah, Max Riedl, Christian Meesters, Andreas Hildebrandt, Thomas Efferth
Summary: This study identified candidate compounds with low binding affinity through virtual drug screening and molecular docking procedures, which may serve as potential drug candidates for treating COVID-19 and other coronavirus infections.
Article
Chemistry, Multidisciplinary
Jennifer E. E. Moises, Christof Regl, Arthur Hinterholzer, Christian G. G. Huber, Mario Schubert
Summary: Glycation is a non-enzymatic and spontaneous post-translational modification that needs to be carefully monitored. 2D NMR spectroscopy can be used to identify glucose-induced glycation products.
PHARMACEUTICAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Christoph Holzner, Katharina Boettinger, Constantin Bloechl, Christian G. Huber, Sven O. Dahms, Elfriede Dall, Hans Brandstetter
Summary: The cysteine protease legumain can translocate to the cell surface through interaction with the RGD-dependent integrin receptor alpha V beta 3. Legumain processes the C-terminal linker region of the TrkB ectodomain, affecting TrkB-BDNF signaling. Legumain-processed TrkB still binds BDNF, suggesting a potential scavenger role of soluble TrkB towards BDNF.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Laura Urwanisch, Michael Stefan Unger, Helene Sieberer, Hieu-Hoa Dang, Theresa Neuper, Christof Regl, Julia Vetter, Susanne Schaller, Stephan M. Winkler, Emanuela Kerschbamer, Christian X. Weichenberger, Peter W. Krenn, Michela Luciano, Lisa Pleyer, Richard Greil, Christian G. Huber, Fritz Aberger, Jutta Horejs-Hoeck
Summary: Epigenetic alterations play a significant role in cancer development, and the reversible nature of epigenetic changes makes them attractive targets for therapy. In this study, the authors investigated the expression of HDAC genes in AML patients and cell lines, and the effects of the HDAC inhibitor TMP269 on AML cells. They found significant overexpression of various HDACs in AML and observed that TMP269 treatment had effects on the proteome and growth of AML cells. Additionally, they demonstrated that the combination of TMP269 and venetoclax resulted in enhanced cell apoptosis. These findings highlight the potential of TMP269 as a therapeutic compound for AML.
Article
Chemistry, Analytical
Fiammetta Di Marco, Gabriele Bluemel, Constantin Bloechl, Manfred Wuhrer, Christian G. Huber
Summary: This study characterized gonadotropins using mass spectrometry and identified over a thousand different glycoforms, highlighting the importance of glycosylation patterns for quality control of biopharmaceuticals.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biochemistry & Molecular Biology
Pauline Defant, Christof Regl, Christian G. Huber, Mario Schubert
Summary: This article presents a study on glycation, a non-enzymatic modification that occurs when reducing sugars react with free amines in protein side chains. The researchers conducted a detailed NMR analysis to identify the correlation patterns of the Amadori product formed between proteins and maltose. The results demonstrate that NMR spectroscopy is an independent method for detecting maltose-based glycation, which can be validated with other methods.
JOURNAL OF BIOMOLECULAR NMR
(2023)
Article
Chemistry, Medicinal
Alaa Alhayek, Ahmed S. Abdelsamie, Esther Schoenauer, Virgyl Camberlein, Evelyn Hutterer, Gernot Posselt, Jamil Serwanja, Constantin Bloechl, Christian G. Huber, Jorg Haupenthal, Hans Brandstetter, Silja Wessler, Anna K. H. Hirsch
Summary: This study reports the discovery of two potent and chemically stable bacterial collagenase inhibitors, synthesized and FDA-approved diphosphonates and hydroxamates. These compounds showed high activity against clostridial and bacillary collagenases, demonstrating antibacterial effects.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)