Article
Biotechnology & Applied Microbiology
Luis A. Ibarra-Sanchez, Wentao Kong, Ting Lu, Michael J. Miller
Summary: This study utilized synthetic biology strategies to create a series of nisin A derivatives by substituting residues with positively charged amino acids, aiming to enhance control of L. monocytogenes in QF. The results showed that while nisin derivatives exhibited reduced antilisterial activity compared to nisin A, they were more stable under QF-like conditions, suggesting the potential for stabilized and enhanced control of L. monocytogenes in QF.
Article
Food Science & Technology
Anran Dong, Alesana Malo, Mabel Leong, Van T. T. Ho, Mark S. Turner
Summary: Listeria monocytogenes is a potentially deadly pathogen that can be controlled using a fermentate produced by nisin-producing Lactococcus lactis strain. A transposon screen was used to isolate a nisin-negative mutant which demonstrated a complete loss of anti-L. monocytogenes activity, showing the importance of nisin production in controlling this pathogen.
Article
Food Science & Technology
A. Morgan, D. Darby, T. Bruce, A. Romero, K. Cooksey
Summary: Training alone is not sufficient to stop the spread of L. monocytogenes. Antimicrobial packaging using nisin is an effective approach, but there is a gap between research laboratories and packaging companies. The developed coatings show antimicrobial activity against L. monocytogenes.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Food Science & Technology
Mengjie Wu, Qingli Dong, Hui Yan, Yiyang Song, Yangtai Liu, Takashi Hirata, Zhuosi Li
Summary: This study evaluated the combined effect of Nisin and sesamol on the growth of Listeria monocytogenes in yellowfin tuna fillet. The results showed that the combined use of NS and SE significantly reduced the growth potential of L. monocytogenes and did not significantly change the texture and color characteristics of tuna fillets.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Food Science & Technology
Mengjie Wu, Qingli Dong, Xia Song, Li Xu, Xuejuan Xia, Muhammad Zohaib Aslam, Yue Ma, Xiaojie Qin, Xiang Wang, Yangtai Liu, Biyao Xu, Hong Liu, Hua Cai, Takashi Hirata, Zhuosi Li
Summary: Nisin has been used as a food preservative against Listeria monocytogenes, but its effectiveness is limited. Combining it with sesamol improves its antibacterial activity. In this study, the combination of nisin and sesamol showed higher inhibition of L. monocytogenes than either compound alone, and caused more significant changes and damage to cell structure and morphology. This suggests that combining nisin and sesamol may be a promising alternative for controlling L. monocytogenes contamination in food.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Food Science & Technology
Danielle Nader Furtado, Bernadette Dora Gombossy de Melo Franco, Svetoslav Dimitrov Todorov
Summary: Differentiated Lactococcus lactis bacteriocin producers' strains using repPCR technique. The stability of bacteriocin was found to be influenced by applied chemicals, pH, and temperature. The addition of bacteriocin completely inhibited the growth of Listeria monocytogenes ScottA, Lactobacillus sakei ATCC 15521, or Enterococcus faecium ATCC 19434 in the study.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Fisheries
Fatemeh Mohajer, Saeid Khanzadi, Kobra Keykhosravy, Seyyed Mohammad Ali Noori, Mohammad Azizzadeh, Mohammad Hashemi
Summary: The study showed that the edible gelatin nanogel coating containing thymol and nisin had a significant inhibitory effect on bacterial growth in fish fillets, with reductions in total viable count, lactic acid bacteria count, among others. Additionally, the separate use of thymol and nisin also led to significant inhibition of bacterial growth compared to the control.
AQUACULTURE RESEARCH
(2021)
Article
Microbiology
Pitchaya Santativongchai, Phitsanu Tulayakul, Byeonghwa Jeon
Summary: This study aims to develop a synergistic approach to better control Listeria biofilms using nisin, in combination with gallic-acid-rich food plant extracts. The results showed that gallic acid significantly decreased the level of biofilm formation in L. monocytogenes, while ethyl gallate, propyl gallate, and lauryl gallate enhanced biofilm production. Sage extracts enhanced the antibiofilm activity of nisin against L. monocytogenes, but the other extracts increased biofilm formation.
Article
Chemistry, Physical
Mayssane Hage, Nour-Eddine Chihib, Marwan Abdallah, Simon Khelissa, Beatrice Crocco, Hikmat Akoum, Fouad Bentiss, Charafeddine Jama
Summary: The study found that functionalizing stainless steel surfaces with nisin, particularly when linked by its amino group, showed efficient antibacterial activity. Immobilizing nisin onto surfaces resulted in a significant reduction in bacterial count, with a tight relation observed between surface topography, nisin conformation, and antibacterial activity.
SURFACES AND INTERFACES
(2021)
Article
Microbiology
Satoshi Kukuminato, Kento Koyama, Shigenobu Koseki
Summary: Novel melanoidins with high antibacterial activity were produced by the Maillard reaction using various combinations of reducing sugars and amino acids. These melanoidins showed effective growth inhibition against various bacteria, including Listeria monocytogenes, Bacillus cereus, and Salmonella enterica Typhimurium.
MICROBIOLOGY SPECTRUM
(2021)
Article
Food Science & Technology
Fangting Bai, Du Guo, Yueyi Wang, Shanbo Zhang, Jiahui Li, Kangkang Zhi, Chao Shi, Xiaodong Xia
Summary: This study investigated the combined bactericidal effect of nisin and thymoquinone on Listeria monocytogenes in Tryptone Soy Broth and sterilized milk. The combination led to a greater reduction in bacterial number and appeared to cause early injury to the bacteria. Nisin and thymoquinone acted on the bacteria in different growth phases and demonstrated a reversible hydrogen-bonding interaction.
Article
Food Science & Technology
Jae-Hyun Yoon, Do-Young Jeong, Su-Bin Lee, Songyi Choi, Myeong-In Jeong, Sun-Young Lee, Se-Ri Kim
Summary: The study showed that dipping king oyster mushrooms in 3% malic acid, combined with 40 kHz ultrasound and 0.1% nisin, effectively reduced Listeria monocytogenes. Further reduction was observed when all three treatments were combined, leading to significant collapse in bacterial cells.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Ezequiel Hernandez-Mendoza, Etna Aida Pena-Ramos, Vijay K. Juneja, Martin Valenzuela-Melendres, Maria Susana Scheuren-Acevedo, Marangeli Osoria
Summary: Mild cooking thermal treatments can be detrimental to ground meat dishes like meatballs in chipotle sauce, especially when salt levels are reduced. Listeria monocytogenes, a thermoresistant pathogen, is a concern in ready-to-eat foods. However, nisin, due to its thermal stability, can be a beneficial alternative for effectively inactivating L. monocytogenes and ensuring meat safety.
JOURNAL OF FOOD PROTECTION
(2023)
Article
Microbiology
Francis Muchaamba, Joseph Wambui, Roger Stephan, Taurai Tasara
Summary: Csps play an important role in L. monocytogenes resistance to nisin stress, with a hierarchy of importance in csp gene contributions: cspD > cspB > cspA. Elimination of either cspA or cspB can improve L. monocytogenes' tolerance to nisin stress. Furthermore, Csp deficiency alters the expression of multiple genes, increasing sensitivity to cell-envelope-targeting stressors.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Food Science & Technology
Bryce R. Hales, Marie K. Walsh, Luis J. Bastarrachea
Summary: The synergistic effect of high-intensity ultrasound, UV-A light, and natural antimicrobials on microbial inactivation in milk was studied. The combination resulted in a reduction of up to 99.99% in the initial microbial population, with further reduction possible after 24 hours of refrigerated storage. The treatments had marginal effect on the color and pH of the milk.
JOURNAL OF FOOD PROCESSING AND PRESERVATION
(2022)
Review
Microbiology
Liam H. Walsh, Mairead Coakley, Aaron M. Walsh, Paul W. O'Toole, Paul D. Cotter
Summary: High-throughput DNA sequencing technologies are revolutionizing our understanding of microbial ecosystems, especially those associated with fermented foods. Metagenomic and metatranscriptomic approaches are being used to investigate the characteristics of microbial communities, requiring the development of appropriate bioinformatic tools to interpret the data.
CRITICAL REVIEWS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Amel Sami, Imad Elimairi, C. Anthony Ryan, R. Paul Ross, Catherine Stanton
Summary: This study investigates the impact of the smokeless tobacco product Toombak on the body's cortisol response and analyzes the psycho-dependency level of Toombak users. The results show that Toombak users have significantly lower hair cortisol levels compared to non-users, and they exhibit high levels of psycho-dependency and nicotine tolerance.
Article
Microbiology
Jose Guilherme Prado Martin, Joao Marcos Maia Silva, Isabel Cristina da Rocha Cesar, Meiriele da Silva, Samara Aparecida Santana, Tomas Gomes Reis Veloso, Jonas Guimaraes e Silva, Celia Lucia de Luces Fortes Ferreira, John Leech, Paul D. Cotter
Summary: Canastra cheese, the most famous artisanal cheese in Brazil, has preserved autochthonous fungi on its surface during production due to their sensory characteristics. This study analyzed the mycobiota of Canastra cheeses produced in different farms and cities during two seasons. The fungal composition significantly varied with the season, city, and farm, but not with altitude. Insights from this study are crucial in light of new regulations in Brazil, allowing for novel approaches in cheese production.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Amy H. H. Fitzpatrick, Agnieszka Rupnik, Helen O'Shea, Fiona Crispie, Paul D. D. Cotter, Sinead Keaveney
Summary: In this study, a novel high-throughput sequencing method for the genotypic characterization of norovirus in oysters was evaluated. It was found that the combination of the reverse transcriptase LunaScript and the DNA polymerase AmpliTaq Gold provided the best results. This method accurately detects and characterizes norovirus populations in oysters and contributes to the surveillance of norovirus in the environment.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Oliver W. Bayfield, Andrey N. Shkoporov, Natalya Yutin, Ekaterina V. Khokhlova, Jake L. R. Smith, Dorothy E. D. P. Hawkins, Eugene V. Koonin, Colin Hill, Alfred A. Antson
Summary: CrAssphage and related crassviruses are the most abundant viruses in the human gut, with some individuals having up to 95% of viral sequences from these viruses. By reconstructing Bacteroides intestinalis virus fcrAss001 using cryo-electron microscopy, we gained insights into the structure and function of its proteins. The muzzle protein forms a new fold called the 'crass fold' and acts as a gatekeeper for controlling cargo release. Additionally, the fcrAss001 virus provides ample storage space for cargo proteins in both the capsid and the tail, with a mechanism for protein ejection involving partial unfolding during extrusion through the tail.
Article
Multidisciplinary Sciences
Catherine O'Reilly, Ghjuvan M. Grimaud, Mairead Coakley, Paula M. O'Connor, Harsh Mathur, Veronica L. Peterson, Ciara M. O'Donovan, Peadar G. Lawlor, Paul D. Cotter, Catherine Stanton, Mary C. Rea, Colin Hill, R. Paul Ross
Summary: Nisin, a broad spectrum bacteriocin, can survive intact through the pig gastrointestinal tract and affect the composition and functioning of the microbiota. It leads to reversible changes in the abundance of Gram positive and Gram negative bacteria, as well as the synthesis pathways of short chain fatty acids. These findings demonstrate the potential of bacteriocins like nisin to shape mammalian microbiomes and impact the functionality of the community.
SCIENTIFIC REPORTS
(2023)
Article
Microbiology
Gabriel Vinderola, Paul D. Cotter, Miguel Freitas, Miguel Gueimonde, Hannah D. Holscher, Patricia Ruas-Madiedo, Seppo Salminen, Kelly S. Swanson, Mary Ellen Sanders, Christopher J. Cifelli
Summary: Fermented foods are often mistaken for probiotics, but there are strict criteria for a fermented food to be considered a 'biotic' including documented health benefit and sufficient product characterization. The terms 'fermented food' and 'probiotic' cannot be used interchangeably. The ability of fermented foods to deliver biotics depends on the level of characterization, reproducibility of the process, evidence of health benefits, and testing for probiotic, prebiotic, synbiotic, and postbiotic capacities.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Instruments & Instrumentation
Aoibhin Ryan, Pratikkumar Patel, Poonam Ratrey, Paula M. O'Connor, Julie O'Sullivan, R. Paul Ross, Colin Hill, Sarah P. Hudson
Summary: Chronic wounds have a global impact and their healing is being hindered by antimicrobial-resistant bacterial infections like MRSA. A study proposes the use of a lacticin 3147 solid lipid nanoparticle gel as a topical treatment for S. aureus and MRSA wound infections. Encapsulation of lacticin 3147 into solid lipid nanoparticles improved its physicochemical properties and resulted in a long-lasting gel with increased activity against S. aureus.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2023)
Article
Agriculture, Dairy & Animal Science
Chloe Matthews, Aaron M. Walsh, Stephen V. Gordon, Bryan Markey, Paul D. Cotter, Jim O' Mahony
Summary: This study investigated the changes in the faecal microbiome of cattle exposed to MAP compared to a control group. Significant differences in taxonomic diversity and composition were observed at 3 months post inoculation, including changes in the relative abundance of Methanobrevibacter and Bifidobacterium genera.
Article
Gastroenterology & Hepatology
Colin Buttimer, Ekaterina V. Khokhlova, Lisa Stein, Cara M. Hueston, Bianca Govi, Lorraine A. Draper, R. Paul Ross, Andrey N. Shkoporov, Colin Hill
Summary: This study isolates and characterizes six bacteriophages that can infect Ruminococcus gnavus, a gut microbe associated with inflammatory bowel disease. The phages have a temperate lifestyle and can coexist with the host bacterium without causing a significant reduction in its abundance. Analysis of gut virome data reveals a high abundance of these phages in individuals with IBD. This work provides insight into the interaction between phages and R. gnavus in the human gut microbiome.
Article
Chemistry, Medicinal
Shona Uniacke-Lowe, Fergus W. J. Collins, Colin Hill, R. Paul Ross
Summary: With the increase in antimicrobial resistance, the deep-sea fish microbiome can serve as a potential source of antimicrobials, including bacteriocins. In this study, bacterial isolates recovered from deep-sea fish were screened for antimicrobial activity and their genomic DNA was analyzed for bacteriocin and other antimicrobial metabolite genes. The study identified 147 candidate biosynthetic gene clusters, including 35 bacteriocin clusters, and discovered unique bacteriocin gene clusters encoding novel peptides. This dual approach provided insights into the bacteriocinogenic potential and antimicrobial molecules of the deep-sea isolates.
Article
Food Science & Technology
Friederike Uhlig, Alicja K. Warda, Cara M. Hueston, Lorraine A. Draper, Gilles Chauviere, Erik Eckhardt, Colin Hill, Niall P. Hyland
Summary: Lactobacillus LB, generated through fermentation, has beneficial effects on intestinal ion transport and motility, alleviating acute diarrhea and irritable bowel syndrome symptoms. Low-lactose Lactobacillus LB (LL-LB) increases baseline short-circuit current and is partially mediated by sodium-D-glucose transporter 1. Preparations produced from a single strain fermentate of L. fermentum have a stronger effect on baseline short-circuit current and carbachol-induced contractions compared to LL-LB.
JOURNAL OF FUNCTIONAL FOODS
(2023)
Article
Medicine, General & Internal
David Healy, Shuo Wang, Ghjuvan Grimaud, Alicja Katarzyna Warda, Paul Ross, Catherine Stanton, Eugene M. Dempsey
Summary: This study aims to investigate the changes in microbiome during the admission of preterm infants and examine the relationship between these changes and different management practices, such as the use of antibiotics, advancement of feeds, and administration of probiotics.
Article
Multidisciplinary Sciences
Harsh Mathur, Monica A. Mechoud, Chloe Matthews, Cathy Lordan, Jamie A. Fitzgerald, Tom Beresford, Paul D. Cotter
Summary: The development of a high-throughput system based on the Applikon micro-Matrix bioreactor for screening gut microbiome modulation is reported. However, Escherichia coli blooms are a common problem encountered in this system. The study provides optimization steps and approaches to attenuate E. coli blooms in such ex vivo colon model experiments.
Meeting Abstract
Nutrition & Dietetics
A. Iyer, A. Mukherjee, B. Gomez-Sala, E. O'Connor, J. G. Kenny, P. D. Cotter
PROCEEDINGS OF THE NUTRITION SOCIETY
(2023)
