Article
Veterinary Sciences
Brigida Kussumoto de Alcantara, Michele Lunardi, Alais Maria Dall Agnol, Alice Fernandes Alfieri, Amauri Alcindo Alfieri
Summary: This study developed a standardized SYBR Green-based quantitative PCR for detecting and quantifying bovine papillomavirus 2 and 13 E6 oncogenes in urinary bladder samples from cattle. Results showed detection of these viruses in bladder samples with tumors but not in healthy bladder mucosa samples. The quantitative PCR assay was proven to be a sensitive and specific tool for identifying and quantifying the E6 ORF of these viruses in clinical samples, confirming previous studies and representing the first detection of bovine papillomavirus 13 DNA in malignant bladder lesions of cattle from Brazil.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Microbiology
Masaki Maezawa, Yuki Fujii, Masataka Akagami, Junko Kawakami, Hisashi Inokuma
Summary: In this study, BoLA-DRB3 genotyping was performed in 59 EBL cattle younger than 3 years and 69 EBL cattle older than 3 years. The BoLA-DRB3*15:01 allele was identified as a susceptibility allele for early EBL onset. The BoLA-DRB3*15:01/*15:01 and BoLA-DRB3*15:01/other genotypes were found to be early EBL onset susceptibility genotypes.
VETERINARY MICROBIOLOGY
(2023)
Article
Infectious Diseases
Francesca De Falco, Federica Corrado, Anna Cutarelli, Leonardo Leonardi, Sante Roperto
Summary: This study compared the levels of BPV DNA in bovine samples using ddPCR and qPCR, finding that ddPCR was more sensitive and accurate than qPCR, allowing the detection of more BPV genotypes.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Veterinary Sciences
Toshiaki Inenaga, Koh Fukuoka, Mikiya Sumida, Sakiko Aiba, Kohei Nishikaku, Yuta Matsuno, Tomoko Kobayashi, Kazuhiko Imakawa
Summary: This study analyzed the occurrence of bovine leukemia (BL) in the Kumamoto strain of Japanese Brown cattle and found that the number of BL cases was low in this population. The proviral load (PVL) of BLV-infected JBRK cattle was significantly lower compared to Japanese Black and Holstein-Friesian breeds. The study also revealed that the PVL levels in BLV-infected JBRK cattle were not significantly affected by BoLA-DRB3 alleles and haplotypes.
BMC VETERINARY RESEARCH
(2023)
Article
Veterinary Sciences
T. Kanno, R. Ishihara, H. Mori, T. Tomiyasu, K. Okazaki
Summary: Bovine leukemia virus (BLV) is a pathogen that causes fatal enzootic bovine leukosis (EBL) and reduces milk production, reproductive efficiency, carcass weight, and longevity in dairy cows. The amino acid at position 233 in Tax protein plays a crucial role in leukemogenesis. This study found that BLV infection increased the risk of involuntary culling and mortality in Japanese Black (JB) cattle, and infection with L233-Tax-carrying virus had a greater impact. The findings highlight the importance of amino acid 233 in Tax in preventing economic losses due to EBL in JB cattle.
RESEARCH IN VETERINARY SCIENCE
(2023)
Article
Veterinary Sciences
Hirohisa Mekata, Mari Yamamoto
Summary: This study aimed to identify novel SNPs related to resistance to BLV. The research revealed a novel SNP located on the SPATA16 gene-coding region on bovine chromosome 1 associated with BLV resistance, with cattle carrying the minor allele showing lower PVL levels.
VETERINARY SCIENCES
(2022)
Article
Immunology
Yakup Yildirim, Mehmet Kale, Ozlem Ozmen, Abdurrahman Anil Cagirgan, Sibel Hasircioglu, Ali Kucuk, Aysegul Usta, Sevinc Sokel
Summary: In this study, the papillomaviruses responsible for teat papillomas in cattle were identified and the tissue damage caused by the virus was examined. Sequence analysis also revealed different types of papillomaviruses.
MICROBIAL PATHOGENESIS
(2022)
Article
Veterinary Sciences
Maria Longeri, Valeria Russo, Maria Giuseppina Strillacci, Antonella Perillo, Michela Carisetti, Maria Cristina Cozzi, Benedetto Neola, Sante Roperto
Summary: The study showed that the DRB3*22 allele in healthy cattle may have a protective effect against bladder tumors associated with papillomavirus infection. Animals carrying the DRB3*011:01 allele were found to have a lower risk of BPV infection and potentially a reduced risk of bladder tumors.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Veterinary Sciences
Takafumi Tomiyasu, Hiroshi Mori, Katsunori Okazaki
Summary: A nationwide surveillance of Japanese Black cattle revealed that the occurrence of bovine leukemia virus (BLV) is associated with the amino acid at position 233 in the Tax protein. The study also found that BLV carrying L233-Tax is more common in animals born via embryo transfer and in dairy areas. These findings contribute to the prevention of enzootic bovine leukosis (EBL).
JOURNAL OF VETERINARY MEDICAL SCIENCE
(2022)
Article
Microbiology
Paulina Pyrek, Michal Bednarski, Jaroslaw Popiel, Magdalena Siedlecka, Magdalena Karwanska
Summary: This study identified bovine papillomavirus (BPV) infections in the Polish cattle population, with types 8 and 7 being the most common. The majority of the observed lesions were rice-grain type, mainly found on one teat. The diversity of BPV types causing teat papillomatosis in Polish cows is large, with nine known types and a new putative type discovered. Additionally, this study revealed the spread of new BPV types, including type 25 and isolates BPV42 and BPV43, among the European cattle population for the first time.
Article
Agriculture, Dairy & Animal Science
Mehmet Kale, Yakup Yildirim, Ozlem Ozmen, Abdurrahman Anil Cagirgan, Sevinc Sokel, Aysegul Usta, Ali Kucuk, Yakup Sinan Orta
Summary: This study aimed to diagnose bovine papillomavirus (BPV) in cows with papillomas and reveal its molecular characterization. Molecular, immunohistochemistry, and transmission electron microscopy methods were used for virus identification. PCR analyses showed the presence of BPV nucleic acid in 70% (14/20) and 90% (18/20) of the samples. Sequence analysis confirmed the phylogenetic relationship between the isolated strains. The study concluded that molecular and phylogenetic studies using type-specific primers are beneficial in understanding the etiology of papillomatosis in cattle.
TROPICAL ANIMAL HEALTH AND PRODUCTION
(2023)
Article
Immunology
Francesca De Falco, Anna Cutarelli, Adriana Florinela Catoi, Barbara Degli Uberti, Bianca Cuccaro, Sante Roperto
Summary: Persistent infection and tumorigenesis by papillomaviruses require manipulation of cellular processes involved in innate immune responses. A study found that a pathway essential for innate immune sensing, the cGAS-STING pathway, is impaired in bovine papillomavirus (BPV) infection, leading to persistent viral infection and tumorigenesis.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Veterinary Sciences
L. Gysens, B. Vanmechelen, P. Maes, A. Martens, M. Haspeslagh
Summary: Infection with bovine papillomavirus (BPV) types 1 and 2 results in equine sarcoid, the most common skin tumor in horses, which differs from BPV-1/-2 induced cutaneous papillomas in cattle. Horse-specific BPV-1/-2 variants have been suggested as a possible explanation for this difference. Through genome sequencing, it was found that there were horse-specific mutations in subpopulations of equine derived BPV-1/-2, but no horse-adapted genetic variants were detected. Cross-species transmission from cattle to horses seems to be an ongoing process.
VETERINARY JOURNAL
(2023)
Article
Veterinary Sciences
S. Khan, H. Akbar, M. Rashid, M. Younas, S. H. Farooqi, F. U. Rehman, A. Badshah, S. Azeem
Summary: This study clinically evaluated two alternative medicines for treating warts in a Sahiwal heifer. The results showed that Thuja occidentalis and Causticum can effectively treat warts in bovines, especially in organic farm settings.
JOURNAL OF THE HELLENIC VETERINARY MEDICAL SOCIETY
(2022)
Article
Veterinary Sciences
Masaki Maezawa, Ken-ichi Watanabe, Kotaro Matsumoto, Yoshiyasu Kobayashi, Haruko Ogawa, Hisashi Inokuma
Summary: This study finds that the methylation rate of the BMP-6 promoter region in EBL beef cattle under 3 years old is significantly higher than in other groups. Hypermethylation of the BMP-6 promoter region may contribute to the early onset of EBL in beef cattle under 3 years old, and specific animal feeding management practices for beef cattle may affect the methylation status of the BMP-6 promoter region.
JOURNAL OF VETERINARY MEDICAL SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
Manuela Martano, Gennaro Altamura, Karen Power, Pierluigi Liguori, Brunella Restucci, Giuseppe Borzacchiello, Paola Maiolino
Summary: Equine sarcoids, caused by bovine papillomaviruses, exhibit an alteration of the autophagic process, leading to the survival of a specific population of fibroblasts that produce more and/or altered collagen in a hypoxic microenvironment.
Article
Biochemistry & Molecular Biology
Nunzio Antonio Cacciola, Angela Salzano, Nunzia D'Onofrio, Tommaso Venneri, Paola De Cicco, Francesco Vinale, Orsolina Petillo, Manuela Martano, Paola Maiolino, Gianluca Neglia, Ciro Campanile, Lorella Severino, Carmine Merola, Francesca Borrelli, Maria Luisa Balestrieri, Giuseppe Campanile
Summary: Pharmacological research on milk whey has shown its potential therapeutic properties in modern medicine. This study focused on whey from Mediterranean buffalo milk and investigated its anticancer effects on colorectal cancer. The results revealed that whey was able to regulate protein expression in apoptotic and necroptotic pathways to inhibit tumor growth. The findings suggest that whey could be a valuable source of biomolecules for the treatment of colorectal cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agronomy
Manuela Martano, Rita Massa, Brunella Restucci, Emilio Caprio, Raffaele Griffo, Karen Power, Paola Maiolino
Summary: The Red Palm Weevil (RPW) is a destructive pest of palms and action plans are recommended to combat its spread due to environmental pollution and resistance to chemicals. This study investigated the effects of 2.45 GHz radiation on the reproductive organs of adult RPWs, which showed degeneration and necrosis of germinal cells with increasing irradiation time and temperature. Additionally, increased temperature resulted in decreased survival time. Microwave radiation could be a useful tool in controlling the reproductive capacity of this insect.
Article
Veterinary Sciences
Gennaro Altamura, Giuseppe Borzacchiello
Summary: The study aims to assess the efficacy of Cetuximab in FOSCC and found that Cetuximab can inhibit the activation of EGFR, reduce cell proliferation and cell viability, and induce cell apoptosis. These results suggest potential anti-cancer activities of Cetuximab in the therapy of FOSCC.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Veterinary Sciences
Florentina Daraban Bocaneti, Gennaro Altamura, Annunziata Corteggio, Oana Irina Tanase, Mihaela Anca Dascalu, Sorin Aurelian Pasca, Ozana Hritcu, Mihai Mares, Giuseppe Borzacchiello
Summary: This study investigated the expression of MMP-1/-8/-13 and TIMP-3 in bovine cutaneous fibropapillomas. The results showed that MMP-8/-13 were overexpressed while MMP-1 was down-regulated in the tumors compared to normal skin samples, along with a decrease in TIMP-3 levels. These findings suggest that an imbalance between MMPs/TIMPs may contribute to increased collagenolytic activity and play a role in ECM changes and tumor development.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Review
Virology
Francesca De Falco, Bianca Cuccaro, Roberta De Tullio, Alberto Alberti, Anna Cutarelli, Esterina De Carlo, Sante Roperto
Summary: Bladder tumors are rare in cattle and are often associated with grazing on bracken fern-infested pasturelands. Bovine papillomaviruses play a crucial role in these tumors. This study aimed to investigate the potential association of ovine papillomavirus infection with bladder carcinogenesis in cattle.
Article
Agriculture, Dairy & Animal Science
Karen Power, Manuela Martano, Nadia Piscopo, Paolo Viola, Gennaro Altamura, Vincenzo Veneziano, Ana Carvajal Uruena, Luigi Esposito
Summary: Recent findings of E. garfiai in wild boars in southern Italy highlight the need for epidemiological data collection on this parasite. This study analyzed the prevalence of E. garfiai in relation to altitude in different provinces of southern and central Italy. The results showed that the parasite is more common at altitudes above 900 m, likely due to a higher presence of earthworms in the soil.
Article
Agriculture, Dairy & Animal Science
Nicolo Montegiove, Leonardo Leonardi, Alessio Cesaretti, Roberto Maria Pellegrino, Alessia Pellegrino, Carla Emiliani, Eleonora Calzoni
Summary: This study analyzed the concentration of biogenic amines and free amino acids in three different chicken-based dry pet food formulations. The results showed that the fresh-meat-based formulation has a lower quantity of potentially harmful biogenic amines compared to the other formulations, making it the preferable choice for dry pet food.
Article
Biochemistry & Molecular Biology
Silvia Trombetti, Nunzia Iaccarino, Patrizia Riccio, Raffaele Sessa, Rosa Catapano, Marcella Salvatore, Stelina Luka, Sergio de Nicola, Paola Izzo, Sante Roperto, Pasqualino Maddalena, Antonio Randazzo, Michela Grosso
Summary: Ferroptosis is a regulated cell death involving lipid peroxidation. GPX4 plays a central role in its regulation. Ferroptosis is connected to lipid metabolism, cancer onset, and drug resistance. GATA-1 isoforms, particularly GATA-1(S), are involved in hematological malignancies. Overexpression of GATA-1(S) prevents ferroptosis in leukemia cells. Targeting ferroptosis is a promising strategy to overcome drug resistance in hematological malignancies.
Article
Veterinary Sciences
Gennaro Altamura, Giuseppe Borzacchiello
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Microbiology
Anna Cutarelli, Francesca Carella, Francesca De Falco, Bianca Cuccaro, Fabio Di Nocera, Donatella Nava, Gionata De Vico, Sante Roperto
Summary: This study investigated the presence of Nocardia crassostreae DNA in mussels from the Mediterranean Sea. The results showed that ddPCR outperformed real-time qPCR in detecting the pathogen, highlighting the high sensitivity and specificity of ddPCR technology in identifying infectious diseases.
Article
Chemistry, Medicinal
Nunzio Antonio Cacciola, Fabrizia Sepe, Salvatore Fioriniello, Orsolina Petillo, Sabrina Margarucci, Marcello Scivicco, Gianfranco Peluso, Anna Balestrieri, Giovanna Bifulco, Brunella Restucci, Lorella Severino
Summary: Canine mammary tumors (CMTs) are common in intact female dogs. In addition to surgery, other therapies may benefit these patients. Exploring new treatments for CMT is promising in veterinary oncology.
Review
Hematology
Arianna Miglio, Valentina Cremonini, Leonardo Leonardi, Elisabetta Manuali, Paola Coliolo, Olimpia Barbato, Cecilia Dall'Aglioa, Maria Teresa Antognoni
Summary: Omics technologies have been extensively used in human medicine, especially in transfusion medicine, but their application in veterinary medicine is still in its early stages. There has been limited research in fields such as oncology, nutrition, cardiology, and nephrology. The veterinary transfusion field lacks available omics data and results.
TRANSFUSION MEDICINE AND HEMOTHERAPY
(2023)
Article
Veterinary Sciences
Gennaro Altamura, Manuela Martano, Anna Matrone, Annunziata Corteggio, Giuseppe Borzacchiello
Summary: Feline oral squamous cell carcinoma (FOSCC) is difficult to treat and new therapeutic strategies are needed. This study found that cetuximab, an anti-epidermal growth factor receptor monoclonal antibody, has inhibitory effects on invasion and metastasis in FOSCC by affecting MMPs and epithelial-mesenchymal transition pathways, potentially improving the management of the disease.
VETERINARY AND COMPARATIVE ONCOLOGY
(2023)
Article
Veterinary Sciences
Gennaro Altamura, Bianca Cuccaro, Claudia Eleni, Carina Strohmayer, Sabine Brandt, Giuseppe Borzacchiello
Summary: Recent evidence suggests a potential association of Felis catus papillomavirus type 2 (FcaPV-2) DNA with feline oral squamous cell carcinoma (FOSCC). This study detected viral DNA in a significant number of FOSCC samples collected in Italy and Austria using type-specific PCR targeting different genes of FcaPV-1/-2/-3/-4/-5/-6. The results confirmed the high prevalence of FcaPV-2 in FOSCC and highlighted potential differences in the circulation rates of FcaPVs among European countries.
JOURNAL OF VETERINARY MEDICAL SCIENCE
(2022)
Article
Pathology
Teruaki Hirota, Kayoko Yonemaru, Mitsunobu Hattori, Mami Murakami, Hiroki Sakai, Akihiro Hirata
Summary: An 11-year-old female Persian cat underwent ovariohysterectomy due to dilation of the uterine cavity with irregular thickening of the wall. The pathological examination revealed multiple nodules originating from the endometrium, invading the myometrium and subserosa, and immunohistochemical staining confirmed the diagnosis of endometrial stromal sarcoma.
JOURNAL OF COMPARATIVE PATHOLOGY
(2024)