Article
Immunology
Caroline Zellmer, Mohamad R. A. Sater, Miriam H. Huntley, Majdi Osman, Scott W. Olesen, Bharat Ramakrishna
Summary: Fecal microbiota transplantation is recommended for multiply recurrent Clostridioides difficile infection, but transmission of Shiga toxin-producing Escherichia coli from donors can lead to adverse events. Improved screening can help prevent future transmission.
CLINICAL INFECTIOUS DISEASES
(2021)
Review
Immunology
Su-bin Hwang, Ramachandran Chelliah, Ji Eun Kang, Momna Rubab, Eric Banan-MwineDaliri, Fazle Elahi, Deog-Hwan Oh
Summary: STEC is a global foodborne bacterial pathogen that can cause severe diarrhea and other critical illnesses, mainly through inflammation caused by Shiga toxins. There is currently no widely accepted treatment for STEC infections, and further research is needed.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Food Science & Technology
Anja Mueller, Diana Seinige, Nils T. Grabowski, Birte Ahlfeld, Min Yue, Corinna Kehrenberg
Summary: Insects as novel foods are gaining popularity in Europe. While the presence of Shiga toxin-producing E. coli (STEC) in edible insects poses a potential health hazard, the majority of isolates belong to phylogenetic group A, mainly commensal E. coli. Moreover, the low resistance rate among the isolates indicates a low risk for the transmission of antimicrobial-resistant E. coli to consumers.
Article
Immunology
Silje N. Ramstad, Arne M. Taxt, Umaer Naseer, Yngvild Wasteson, Jorgen V. Bjornholt, Lin T. Brandal
Summary: The study found that some antibiotics can increase the production of Shiga toxin, while others do not. STEC O104:H4 was the serotype that exhibited the strongest response to antimicrobial exposure with an increase in stx2a transcription and Stx production.
MICROBIAL PATHOGENESIS
(2021)
Article
Environmental Sciences
Kannan Kamala, Shanmugam Rajeshkumar, Pitchiah Sivaperumal
Summary: The study revealed the contamination of Shiga toxin-producing Escherichia coli (STEC) in the marine environment along the Southeast Coast of India, with non-STEC isolates playing a key role in environmental and public health. The findings underscore the need for further research into the pathogenic mechanism of STEC.
MARINE POLLUTION BULLETIN
(2022)
Article
Biotechnology & Applied Microbiology
Alexander Gill, Tanis McMahon, Forest Dussault, Karen Jinneman, Rebecca Lindsey, Haley Martin, Devon Stoneburg, Nancy Strockbine, June Wetherington, Peter Feng
Summary: Two outbreaks of Shiga toxin-producing Escherichia coli O121:H19 associated with wheat flour in the USA and Canada involved strains with delayed lactose utilization (DLU), attributed to the insertion sequence IS600. This insertion might reduce the expression of fi-galactoside permease, resulting in the DLU phenotype. Considering the high probability of DLU is important when using lactose-containing media for the isolation of STEC O121.
Article
Infectious Diseases
Nicola Mangieri, Roberto Foschino, Claudia Picozzi
Summary: The study found that using bacteriophages can effectively reduce the number of E. coli bacteria producing biofilms, thus improving food safety. Preventing biofilm formation is more effective than trying to remove pre-formed biofilms.
Article
Food Science & Technology
Lene Idland, Erik G. Bo-Granquist, Marina Aspholm, Toril Lindback
Summary: Despite lacking scientific evidence, some consumers believe raw milk is more nutritious and immunological, leading to the popularity of unpasteurized cow milk. However, raw milk may pose a risk of STEC infections.
Article
Microbiology
Yujie Zhang, Yen-Te Liao, Alexandra Salvador, Vivian C. H. Wu
Summary: This study genetically characterized two Stx-converting phages induced from environmental STEC strains and found high genomic diversity among Stx-converting phages. The findings provide valuable insights into the dissemination of stx genes among E. coli population via the lysogenization of Stx-converting phages.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Xi Yang, Qian Liu, Hui Sun, Yanwen Xiong, Andreas Matussek, Xiangning Bai
Summary: This study identified Stx2l-STEC strains from raw mutton and beef in China, showing that these strains belonged to the same O8 serogroup with diverse host range and geographical distribution. Genomic analysis revealed genetic relatedness between human-derived and food-derived strains, highlighting the need for further investigation into the epidemiological traits and pathogenic potential of Stx2l-STEC strains.
Article
Food Science & Technology
Xinxia Sui, Xi Yang, Ming Luo, Hua Wang, Qian Liu, Hui Sun, Yujuan Jin, Yannong Wu, Xiangning Bai, Yanwen Xiong
Summary: This study found that Shiga toxin-producing Escherichia coli (STEC) circulating in asymptomatic food handlers is genetically distinct from the strains causing disease, suggesting a low potential for disease-causing.
Article
Food Science & Technology
Agnieszka Necel, Sylwia Bloch, Bozena Nejman-Falenczyk, Aleksandra Dydecka, Gracja Topka-Bielecka, Alicja Wegrzyn, Grzegorz Wegrzyn
Summary: This study proposes a validation system for selecting bacteriophages against STEC contamination, and conducts model experiments to explore their potential applications. The results show that bacteriophage vB_Eco4M-7 has a certain bactericidal effect on STEC, but caution should be taken due to the appearance of phage-resistant bacteria.
Review
Food Science & Technology
Yang Liu, Hatim Thaker, Chunyan Wang, Zhonggao Xu, Min Dong
Summary: Shiga toxin-producing Escherichia coli (STEC)-associated hemolytic uremic syndrome (STEC-HUS) is a clinical syndrome involving hemolytic anemia, thrombocytopenia, and acute kidney injury (AKI). Early diagnosis is crucial for improving prognosis and reducing mortality and sequelae. The review provides an overview of diagnostic methods and preventive and therapeutic strategies for treating STEC-HUS.
Letter
Immunology
Guillaume Geslain, Aurelie Cointe, Philippe Bidet, Celine Courroux, Soumeth Abasse, Patricia Mariani, Stephane Bonacorsi
Summary: We describe fatal meningitis in 2 neonates in France caused by Shiga toxin 1-producing Escherichia coli. Both strains possessed the virulence factors capsular K1 antigen and salmochelin, potentially suggesting a novel hybrid pathotype. Clinicians should be vigilant about emerging pathotypes and develop therapeutic strategies for neonatal E. coli infections.
EMERGING INFECTIOUS DISEASES
(2023)
Article
Microbiology
Magali Fernandez, Maria Laura Casaux, Martin Fraga, Rafael Vignoli, Ines Bado, Pablo Zunino, Ana Umpierrez
Summary: The aim of this study was to characterize pathogenic Escherichia coli isolates from dead newborn calves in Uruguay. Various virulence genes were identified, and a high prevalence of STEC, EPEC, and NTEC infections was found. The potential role of EHEC (stx1/eae/ehxA) in calf mortality was indicated. Antimicrobial resistance and gene transmissibility pose a threat to public health.
Article
Biotechnology & Applied Microbiology
Michelle Qiu Carter, Doris Feng, Hui Hong Li
Article
Food Science & Technology
Michelle Qiu Carter, Zhong Fang Tan, Antares Pham, Diana K. Carychao, Michael B. Cooley
FOODBORNE PATHOGENS AND DISEASE
(2019)
Article
Biotechnology & Applied Microbiology
Daniel S. Munther, Michelle Q. Carter, Claude Aldric, Renata Ivanek, Maria T. Brandl
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2020)
Article
Microbiology
Clifton K. Fagerquist, William J. Zaragoza, Michelle Q. Carter
Article
Biotechnology & Applied Microbiology
Brittany Suttner, Eric R. Johnston, Luis H. Orellana, Luis M. Rodriguez-R, Janet K. Hatt, Diana Carychao, Michelle Q. Carter, Michael B. Cooley, Konstantinos T. Konstantinidis
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Sandy Thao, Maria T. Brandl, Michelle Qiu Carter
Article
Food Science & Technology
Michelle Qiu Carter, Antares Pham, Xiaohua He, Robert Hnasko
FOODBORNE PATHOGENS AND DISEASE
(2020)
Article
Food Science & Technology
Michelle Qiu Carter, Antares Pham, Wen-Xian Du, Xiaohua He
Summary: Research revealed diverse Stx-prophages in STEC O145:H28 strains, which were genotypically indistinguishable. Although all strains belonged to Sequence Type 78 and shared similar characteristics, they exhibited differential responses to various inducers, suggesting a role of host bacteria in regulating Stxs production.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Michelle Qiu Carter, Antares Pham, Steven Huynh, Craig T. Parker, Avalon Miller, Xiaohua He, Bin Hu, Patrick S. G. Chain
Summary: The study revealed significant differences in the methylome between different pathogenic strains of Escherichia coli, with one key reason being the presence of the PstI R-M system. In comparison to deficiency in DNA adenine methylase (Dam), deficiency in the PstI R-M system only had a transcriptional impact on a small number of genes.
Article
Multidisciplinary Sciences
Michelle Qiu Carter, Bin Hu, Patrick S. G. Chain
Summary: This study investigates the role of the PstI R-M system and Dam in the growth and physiology of the STEC strain RM13514 by generating mutant strains. The findings provide valuable insights into methylation-mediated epigenetic regulation in bacteria and its implications in foodborne pathogens and epidemic outbreaks.
Article
Microbiology
Michelle Qiu Carter, Nicole Laniohan, Chien-Chi Lo, Patrick S. G. Chain
Summary: This study examines the pathogenic potential of Shiga toxin-producing Escherichia coli (STEC) O145:H28 in clinical and environmental strains using comparative genomics. The core genes-based tree failed to differentiate between environmental and clinical strains, while the accessory genes-based tree grouped all clinical strains together. Loss-of-function mutations were common in virulence genes related to adherence and secretion systems. Differences in pathogenicity islands and other genetic elements were observed between O145:H28 and reference strains. The study reveals the genetic diversity and evolution of virulence in STEC.
Article
Food Science & Technology
Yan Wang, William M. Hart-Cooper, Reuven Rasooly, Michelle Qiu Carter, William J. Orts, Yongqiang Gu, Xiaohua He
Summary: This study evaluated a newly developed broad-spectrum and reversible antibiotic for its antibiotic activity and effects on Shiga toxin production and global transcription in bacteria. The results showed that the antibiotic did not induce Shiga toxin production and enhanced the expression of stress-responsive genes. Additionally, upregulation of antibiotic resistant and virulence genes was observed in strains treated with the disinfectant. This study contributes to our understanding of the disinfectant's mode of action and improves the use of antibiotics for treating Escherichia coli infections.
Article
Microbiology
Michelle Q. Carter, Antares Pham, Diana K. Carychao, Michael B. Cooley
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2019)
Article
Biotechnology & Applied Microbiology
Michelle Qiu Carter, Maria T. Brandl, Indira T. Kudva, Robab Katani, Matthew R. Moreau, Vivek Kapur
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2018)
Article
Michelle Qiu Carter, Antares Pham
GENOME ANNOUNCEMENTS
(2018)