Article
Virology
Nina Redzic, Ina Benoy, Davy Vanden Broeck, Johannes P. Bogers
Summary: A newly developed assay for HPV genotyping in warts was validated, showing high sensitivity and specificity, and potential for optimizing wart treatment strategies. Multiple HPV infections were detected in 35% of lesions, contradicting previous literature on the prevalence of multiple infections in warts among immunocompetent patients.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Microbiology
Margo Bell, Bo Verberckmoes, Janne Devolder, Heleen Vermandere, Olivier Degomme, Yasmin Medeiros Guimaraes, Luani Rezende Godoy, Elena Ambrosino, Piet Cools, Elizaveta Padalko
Summary: This study demonstrates that the novel Allplex II HPV28 assay has a good diagnostic performance compared to widely used HPV assays. The assay has a user-friendly and automated workflow, an open platform for add-on assays, and provides quick and easy-to-interpret results. It shows potential for simplification and standardization of future diagnostic testing programs.
MICROBIOLOGY SPECTRUM
(2023)
Article
Chemistry, Analytical
Ana Sanchez-Cano, Gisela Ruiz-Vega, Sergi Vicente-Gomez, Erica de la Serna, Elena Sulleiro, Israel Molina, Adrian Sanchez-Montalva, Eva Baldrich
Summary: The new magneto-immunoassay combines magnetic beads, an enzymatic signal amplifier, and chemiluminescence detection to provide fast, sensitive, and quantitative malaria diagnosis with easy user manipulation.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Marianna Martinelli, Chiara Giubbi, Laura Saderi, Rosario Musumeci, Federica Perdoni, Biagio Eugenio Leone, Robert Fruscio, Fabio Landoni, Andrea Piana, Giovanni Sotgiu, Clementina Elvezia Cocuzza
Summary: HPV genotyping and viral load may serve as potential diagnostic biomarkers for high-grade cervical lesions. This study evaluated a new HPV test and found that the distribution of HR-HPV genotypes and viral copy numbers differed between women with high- and low-grade cervical lesions. These findings suggest that HPV genotyping and normalized viral load may help identify women at increased risk of cervical lesions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Luisa Galati, Jean-Damien Combes, Florence Le Calvez-Kelm, Sandrine McKay-Chopin, Nathalie Forey, Mathis Ratel, James McKay, Tim Waterboer, Lea Schroeder, Gary Clifford, Massimo Tommasino, Tarik Gheit
Summary: Human papillomavirus (HPV) circulating tumor DNA (HPV ctDNA) has been proposed as a biomarker for the detection and monitoring of HPV-related cancers. In this study, a bead-based HPV genotyping assay (E7-MPG) was evaluated for its performance in detecting HPV16 ctDNA in plasma samples from women diagnosed with HPV16-positive cervical cancer and premalignant lesions. The sensitivity of the E7-MPG test for HPV16-positive cervical cancer detection was found to be higher than that of droplet digital PCR (ddPCR), and the combination of HPV16 ctDNA and E6 antibodies increased the sensitivity for detection. These results suggest that E7-MPG can serve as a sensitive tool for ctDNA detection of HPV16-positive cervical cancer, and the addition of E6 antibodies improves the detection of cancer.
MICROBIOLOGY SPECTRUM
(2022)
Article
Virology
Fabio Bottari, Anna Daniela Iacobone, Davide Radice, Eleonora Petra Preti, Mario Preti, Dorella Franchi, Andrea Fausto Piana, Maria Teresa Sandri, Rita Passerini
Summary: The study evaluated the performance of the Seegene Anyplex II HPV HR (Anyplex) assay in detecting cervical intraepithelial lesions (CIN) and in post-treatment follow-up, showing potential value in HPV detection and genotyping for stratifying high-risk women and monitoring treatment outcomes for CIN2+ lesions.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Oncology
Mark H. Stoler, Thomas C. Wright Jr, Valentin Parvu, Karen Yanson, Charles K. Cooper, Jeffrey A. Andrews
Summary: The Onclarity cervical cancer screening trial aims to establish the clinical efficacy of the Onclarity HPV assay for extended genotyping. Three-year follow-up data shows that this screening strategy has better sensitivity and a lower colposcopy/lesion ratio compared to baseline data.
GYNECOLOGIC ONCOLOGY
(2023)
Article
Virology
Hiroko Kuroki, Jinichi Sakamoto, Takeo Shibata, Masahiro Takakura, Toshiyuki Sasagawa
Summary: The study showed that in Japan, APTIMA has a higher sensitivity for cervical cancer screening compared to LSIL+ and ASC-US-HC2, as well as for detecting CIN2+ and CIN3+ compared to HC2. HPV genotyping revealed that APTIMA is more effective at identifying high-risk HPV in CIN2/3 patients compared to HC2. Therefore, APTIMA may be more suitable than HC2 for primary HPV screening in Japan.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Virology
Fernanda L. S. G. Santos, Maria C. V. Invencao, Edilaine D. Araujo, Gerlane S. Barros, Marcus V. A. Batista
Summary: This study evaluated the effectiveness of three different PCR-based strategies for HPV detection and genotyping from cervical samples. The results showed that different primer design strategies influenced the sensitivity and specificity of detecting different HPV types. EntroA primers were able to detect HPV in more samples compared to MY09/MY11, while multiplex PCR had higher sensitivity despite targeting a limited number of HPV types.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Raiza P. S. Lucena, Isaac A. M. Frias, Norma Lucena-Silva, Cesar A. S. Andrade, Maria D. L. Oliveira
Summary: HPV is a group of viruses that infect skin and mucosa, typically transmitted through sexual contact. A new electrochemical genosensor has been developed as an alternative method for identifying HPV genotypes, offering quick detection and identification of oncogenic subtypes.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2021)
Article
Virology
Francois Coutlee, Alexandra de Pokomandy, Ann N. Burchell, Mariam El-Zein, Marie-Helene Mayrand, Sophie Rodrigues-Coutlee, Deborah Money, Emilie Comete, Elisabeth McClymont, Danielle Rouleau, Eduardo L. Franco
Summary: This study assessed the agreement between Anyplex II HPV-28 (HPV-28) and linear array HPV genotyping (LA-HPV) for HPV genotype detection. The results showed that HPV-28 performed favorably compared to LA-HPV, but had a higher frequency of positive results for HPV42 and HPV68.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Chemistry, Analytical
Xhensila Shkembi, Vasso Skouridou, Marketa Svobodova, Sandra Leonardo, Abdulaziz S. Bashammakh, Abdulrahman O. Alyoubi, Monica Campas, Ciara K. O'Sullivan
Summary: This study developed an aptamer-based detection method for the rapid and sensitive detection of TTX in pufferfish, using next-generation sequencing technology. The results demonstrated that the developed hybrid antibody-aptamer sandwich assay outperformed several methods reported in the literature and commercial immunoassay kits.
ANALYTICAL CHEMISTRY
(2021)
Article
Oncology
Ariadna Salvado, Ester Miralpeix, Josep M. Sole-Sedeno, Nadwa Kanjou, Belen Lloveras, Xavier Duran, Gemma Mancebo
Summary: The study found that HPV-16 infection and previous HSIL cytology results can predict the risk of persistence or progression of CIN2 lesions. HPV-negative lesions typically regress. Age may not be a key factor in determining the best management strategy.
GYNECOLOGIC ONCOLOGY
(2021)
Article
Health Care Sciences & Services
Rosa Montero-Macias, Pluvio J. Coronado, Nicolas Robillard, David Veyer, Vincent Villefranque, Marie-Aude Le Frere-Belda, Elisabeth Auberger, Pauline Bitolog, Ivana Stankovic, Laurent Belec, Anne-Sophie Bats, Fabrice Lecuru, Helene Pere
Summary: The objective of this study was to determine if an easy-to-use HPV molecular genotyping assay could differentiate HPV tumor metastasis from a new independent primary non-HPV-induced tumor. Using routine multiplex real-time PCR, we found that HPV DNA was detected in the biopsy of distant lesions in 7 out of 8 cases with primary cervical cancer and a new secondary lesion, confirming the diagnosis of cervical cancer metastasis. In the remaining case, no HPV was detected in the lung biopsy, confirming the diagnosis of a new primary lung cancer.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Biology
Hajime Shinoda, Yuya Taguchi, Ryoya Nakagawa, Asami Makino, Sae Okazaki, Masahiro Nakano, Yukiko Muramoto, Chiharu Takahashi, Ikuko Takahashi, Jun Ando, Takeshi Noda, Osamu Nureki, Hiroshi Nishimasu, Rikiya Watanabe
Summary: The study introduces a novel CRISPR-based platform called SATORI, which allows amplification-free digital RNA detection with high sensitivity and specificity in a short period of time. The simultaneous use of multiple guide RNAs enhances sensitivity, enabling the detection of SARS-CoV-2 RNA at low levels.
COMMUNICATIONS BIOLOGY
(2021)