Article
Multidisciplinary Sciences
D. R. Marinowic, G. Zanirati, F. V. F. Rodrigues, M. V. C. Grahl, A. M. Alcara, D. C. Machado, J. C. Da Costa
Summary: A low-cost, highly specific method for SARS-CoV-2 detection has been successfully developed using the SYBR Green RT-PCR method, with the ability to screen out negative cases and high safety and efficacy. Optimization of experimental parameters achieved high specificity of PCR products, providing technical support for large-scale screening.
SCIENTIFIC REPORTS
(2021)
Article
Virology
Jian Yin, Siying Peng, Changning Zhang, Xinyue Li, Fangfang Hu, Wen Chen, Youlin Qiao
Summary: The performance of LiPA-25 and Hybribio-23 in HPV detection and genotyping was compared. The results showed that the two methods had similar outcomes, suggesting that Hybribio-23 can be used for HPV detection and genotyping in epidemiological and vaccine studies worldwide.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Microbiology
Eiseul Kim, Dayoung Kim, Seung-Min Yang, Hae-Yeong Kim
Summary: In this study, a multiplex real-time PCR method was developed to identify and differentiate three closely related species in the Lactobacillus acidophilus group. The method showed high specificity and sensitivity, with a detection limit of 10(1) CFU/ml. It can be used for monitoring these species in probiotic and dairy products.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Immunology
Andrew Pekosz, Valentin Parvu, Maggie Li, Jeffrey C. Andrews, Yukari C. Manabe, Salma Kodsi, Devin S. Gary, Celine Roger-Dalbert, Jeffry Leitch, Charles K. Cooper
Summary: SARS-CoV-2 antigen testing may be positive early in the disease course, aiding in determining infectious risk, with a slightly better detection of infectious virus compared to rt-PCR.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Immunology
Andrew Pekosz, Valentin Parvu, Maggie Li, Jeffrey C. Andrews, Yukari C. Manabe, Salma Kodsi, Devin S. Gary, Celine Roger-Dalbert, Jeffry Leitch, Charles K. Cooper
Summary: Recent studies indicate that SARS-CoV-2 antigen testing demonstrates a higher positive predictive value in the early stages of infection and shows good consistency with culture results. This testing method can significantly aid in determining the risk of carrying infectious virus.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Veterinary Sciences
Dan Yin, Renhao Geng, Hui Lv, Chunhui Bao, Hongxia Shao, Jianqiang Ye, Kun Qian, Aijian Qin
Summary: The real-time PCR assay targeting the A137R gene showed better performance in detecting ASFV from clinical samples compared to the OIE-recommended TaqMan PCR, indicating its potential application in ASF control and eradication programs.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Medicine, Legal
Zhonghao Yu, Quyi Xu, Cheng Xiao, Huan Li, Weibin Wu, Weian Du, Jian Zhao, Hong Liu, Huijun Wang, Chao Liu
Summary: In drowning research, the method of diatom morphology is commonly used to determine the cause of death. However, a new method involving gene detection of phytoplankton has been proposed in this study, showing higher accuracy and faster diagnosis of drowning cases.
FORENSIC SCIENCE INTERNATIONAL
(2021)
Review
Medicine, General & Internal
Ali A. Rabaan, Raghavendra Tirupathi, Anupam A. Sule, Jehad Aldali, Abbas Al Mutair, Saad Alhumaid, Muzaheed, Nitin Gupta, Thoyaja Koritala, Ramesh Adhikari, Muhammad Bilal, Manish Dhawan, Ruchi Tiwari, Saikat Mitra, Talha Bin Emran, Kuldeep Dhama
Summary: Real-time RT-PCR is considered the gold standard confirmatory test for COVID-19, but there is disagreement among scientists about its reliability. Ct values are being used to diagnose or predict SARS-CoV-2 infection and are correlated with viral load and various hematological and biochemical markers. Numerous factors can influence Ct values and their interpretation plays a crucial role in determining viral load and disease severity.
Editorial Material
Oncology
Eduardo Franco
Summary: The American Cancer Society (ACS) believes that the time has come for primary human papillomavirus (HPV) screening in the United States, but it requires educating the public and healthcare providers and overcoming logistical barriers. ACS is taking the lead by utilizing its resources to promote primary HPV screening in the US and sharing best practices from other countries through its Primary HPV Screening Initiative.
Article
Chemistry, Multidisciplinary
Bong Kyun Kim, Sang-A Lee, Minju Park, Eui Ju Jeon, Mi Jung Kim, Jung Min Kim, Heesuk Kim, Seungwon Jung, Sang Kyung Kim
Summary: This article introduces a photonic PCR platform using hydrogel microparticles, which utilizes photothermal nanomaterials as heating elements for rapid and portable POCT. The researchers successfully demonstrate the ability to perform multiplex assays by loading multiple encoded pPIN microparticles in a single reaction.
Article
Medicine, General & Internal
Yanxia Zhao, Heling Bao, Lan Ma, Bo Song, Jiangli Di, Linhong Wang, Yanqiu Gao, Wenhui Ren, Shi Wang, Hai-Jun Wang, Jiuling Wu
Summary: The study evaluated the effectiveness of HPV testing in the national cervical cancer screening programme in China. Results showed that HPV testing had higher CIN2+ detection rate and PPV compared to cytology, with colposcopy referrals differing by income status in different regions.
Review
Medicine, General & Internal
Yilu Zhou, Xiaoyu Shi, Jiaxin Liu, Lina Zhang
Summary: This article aims to summarize the role of different genotypes, multiple infections, especially viral load, in cervical precancerous lesions, in order to guide early intervention.
FRONTIERS IN MEDICINE
(2023)
Article
Biochemical Research Methods
Yong Wang, Ziteng Fu, Xu Guo, Da Zhang, Caixia Bai, Wei Li, Guangqing Liu, Yongdong Li, Shudong Jiang
Summary: A SYBR Green I-based real-time RT-PCR method was developed for clinical diagnosis of feline astroviruses, showing good specificity, sensitivity, and reproducibility. The method has a higher positive detection rate compared to conventional RT-PCR and is cheap and effective for large-scale clinical testing and epidemiological investigation.
JOURNAL OF VIROLOGICAL METHODS
(2021)
Article
Medicine, General & Internal
Ayesha Nayyar, Suhaib Ahmed
Summary: The study aimed to optimize and evaluate real time PCR of Single Nucleotide Polymorphism by SYBR Green for detecting donor chimerism after haematopoietic stem cell transplantation. The results showed that this method was sensitive and effective in detecting chimerism in post-transplant patients with various haematological disorders.
PAKISTAN JOURNAL OF MEDICAL SCIENCES
(2021)
Article
Biochemical Research Methods
Liting Sun, Zhiqing Xu, Junhuang Wu, Yongqiu Cui, Xu Guo, Fazhi Xu, Yongdong Li, Yong Wang
Summary: A novel duplex SYBR Green I-based qPCR assay was developed for the rapid and simultaneous detection of FPV and FCoV, demonstrating sensitivity and specificity in detecting these viruses.
JOURNAL OF VIROLOGICAL METHODS
(2021)
Article
Cell Biology
Judith V. M. G. Bovee, Fleur Webster, Fernanda Amary, Daniel Baumhoer, J. L. (Hans) Bloem, Julia A. Bridge, Justin M. M. Cates, Enrique de Alava, Angelo Paolo Dei Tos, Kevin B. Jones, Annabelle Mahar, G. Petur Nielsen, Alberto Righi, Andrew J. Wagner, Akihiko Yoshida, Christopher D. M. Fletcher
Summary: This study presents international consensus-based datasets for the pathology reporting of bone sarcoma biopsy and resection specimens. These datasets were developed under the ICCR and aim to improve the quality and standardization of pathology reporting, thus enhancing the management of bone sarcoma patients.
Correction
Multidisciplinary Sciences
Ludmil B. Alexandrov, Jaegil Kim, Nicholas J. Haradhvala, Mi Ni Huang, Alvin Wei Tian Ng, Yang Wu, Arnoud Boot, Kyle R. Covington, Dmitry A. Gordenin, Erik N. Bergstrom, S. M. Ashiqul Islam, Nuria Lopez-Bigas, Leszek J. Klimczak, John R. McPherson, Sandro Morganella, Radhakrishnan Sabarinathan, David A. Wheeler, Ville Mustonen, Gad Getz, Steven G. Rozen, Michael R. Stratton
Correction
Multidisciplinary Sciences
Claudia Calabrese, Natalie R. Davidson, Deniz Demircioglu, Nuno A. Fonseca, Yao He, Andre Kahles, Kjong-Van Lehmann, Fenglin Liu, Yuichi Shiraishi, Cameron M. Soulette, Lara Urban, Liliana Greger, Siliang Li, Dongbing Liu, Marc D. Perry, Qian Xiang, Fan Zhang, Junjun Zhang, Peter Bailey, Serap Erkek, Katherine A. Hoadley, Yong Hou, Matthew R. Huska, Helena Kilpinen, Jan O. Korbel, Maximillian G. Marin, Julia Markowski, Tannistha Nandi, Qiang Pan-Hammarstrom, Chandra Sekhar Pedamallu, Reiner Siebert, Stefan G. Stark, Hong Su, Patrick Tan, Sebastian M. Waszak, Christina Yung, Shida Zhu, Philip Awadalla, Chad J. Creighton, Matthew Meyerson, B. F. Francis Ouellette, Kui Wu, Huanming Yang, Alvis Brazma, Angela N. Brooks, Jonathan Goeke, Gunnar Raetsch, Roland F. Schwarz, Oliver Stegle, Zemin Zhang
Correction
Multidisciplinary Sciences
Yilong Li, Nicola D. Roberts, Jeremiah A. Wala, Ofer Shapira, Steven E. Schumacher, Kiran Kumar, Ekta Khurana, Sebastian Waszak, Jan O. Korbel, James E. Haber, Marcin Imielinski, Joachim Weischenfeldt, Rameen Beroukhim, Peter J. Campbell
Article
Immunology
Laura Quotti Tubi, Elisa Mandato, Sara Canovas Nunes, Arash Arjomand, Fortunato Zaffino, Sabrina Manni, Alessandro Casellato, Paolo Macaccaro, Nicola Vitulo, Sara Zumerle, Odile Filhol, Brigitte Boldyreff, Christian W. Siebel, Antonella Viola, Giorgio Valle, Federica Mainoldi, Stefano Casola, Valeria Cancila, Alessandro Gulino, Claudio Tripodo, Marco Pizzi, Angelo Paolo Dei Tos, Livio Trentin, Gianpietro Semenzato, Francesco Piazza
Summary: The role of CK2 in B-cell development and activation is not well understood. Using a CK2 beta(KO) mouse model, we found that CK2 beta(KO) mice exhibit increased marginal zone (MZ) B cells and reduced follicular B cells, suggesting a role for CK2 in the regulation of BCR and NOTCH2 signaling pathways. Further analysis revealed enhanced activation of the NOTCH2 pathway in CK2 beta(KO) mice, supporting MZ B-cell development. Additionally, CK2 beta(KO) mice showed alterations in immune response and B-cell activation processes. In vitro assays demonstrated impaired signaling downstream of BCR, Toll-like receptor, CD40, and IL-4R in B cells lacking CK2 beta.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Oncology
Laureline Wetterwald, Nicolo Riggi, Anastasios Kyriazoglou, Giovanni Dei Tos, Angelo Dei Tos, Antonia Digklia
Summary: Clear cell sarcoma (CCS) is an ultrarare soft tissue sarcoma with a poor prognosis. The standard treatment for localized CCS is surgical excision with or without radiotherapy. The treatment options for unresectable CCS are limited despite the lack of scientific evidence.
EXPERT REVIEW OF ANTICANCER THERAPY
(2023)
Article
Hematology
Marco Pizzi, Marta Sbaraglia, Luca Dal Santo, Debora De Bartolo, Luisa Santoro, Federico Scarmozzino, Lara Mussolin, Marta Pillon, Francesco Piazza, Livio Trentin, Angelo Paolo Dei Tos
Summary: This study retrospectively reviewed 43 paired LNCB/SEB samples and found that LNCB had limited diagnostic value, with a significant number of diagnostic errors and delay. Therefore, SEB should still be considered as the gold standard procedure.
INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Lisa Padroni, Laura De Marco, Lucia Dansero, Valentina Fiano, Lorenzo Milani, Paolo Vasapolli, Luca Manfredi, Saverio Caini, Claudia Agnoli, Fulvio Ricceri, Carlotta Sacerdote
Summary: Breast cancer is a multifactorial disease influenced by genetic predisposition and environmental exposures. MicroRNAs, a group of small non-coding RNA molecules, may act as tumor suppressor genes or oncogenes and play a role in cancer risk. A systematic review and meta-analysis were conducted to identify circulating microRNAs associated with breast cancer diagnosis, with emphasis on methodological issues. The analysis included 75 studies, and meta-analysis was performed for microRNAs analyzed in at least three independent studies. The study found that MIR21, MIR155, and MIR10b were differentially expressed in breast cancer patients compared to healthy controls, but there was little consistency among the included studies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pathology
Federica Miglietta, Valentina Carraro, Ottavia Amato, Gaia Griguolo, Michele Bottosso, Giada Munari, Giovanni Zarrilli, Marcello Lo Mele, Caterina Barbieri, Angelo Paolo Dei Tos, Valentina Guarneri, Maria Vittoria Dieci, Matteo Fassan
Summary: This study aims to track the dynamics of the PI3K/PTEN/mTOR pathway in HR+/HER2- breast cancer patients receiving neoadjuvant chemotherapy. The results show that this pathway is highly dynamic and may play a crucial role in shaping the molecular landscape of breast cancer with residual disease after chemotherapy.
JOURNAL OF CLINICAL PATHOLOGY
(2023)
Article
Surgery
Gaya Spolverato, Matteo Fassan, Giulia Capelli, Melania Scarpa, Silvia Negro, Valentina Chiminazzo, Andromachi Kotsafti, Imerio Angriman, Michela Campi, Ottavia De Simoni, Cesare Ruffolo, Stepanyan Astghik, Chiara Vignotto, Federico Scognamiglio, Giulia Becherucci, Giorgio Rivella, Francesco Marchegiani, Luca Facci, Francesca Bergamo, Stefano Brignola, Gianluca Businello, Vincenza Guzzardo, Luca Dal Santo, Roberta Salmaso, Marco Massani, Anna Pozza, Ivana Cataldo, Tommaso Stecca, Angelo Paolo Dei Tos, Vittorina Zagonel, Pierluigi Pilati, Boris Franzato, Antonio Scapinello, Giovanni Pirozzolo, Alfonso Recordare, Roberto Merenda, Giovanni Bordignon, Silvio Guerriero, Chiara Romiti, Giuseppe Portale, Chiara Cipollari, Maurizio Zizzo, Andrea Porzionato, Marco Agostini, Francesco Cavallin, Barbara Di Camillo, Romeo Bardini, Isacco Maretto, Ignazio Castagliuolo, Salvatore Pucciarelli, Marco Scarpa
Summary: This study evaluated the sex differences in the immune microenvironment of rectal cancer patients and found that male patients had more frequent oncogene mutations associated with lower expression of T-cell activation genes, while female patients had more Th1 cells and cytotoxic T cells in the healthy mucosa, suggesting a potentially better immune response to the tumor.
INTERNATIONAL JOURNAL OF SURGERY
(2023)
Review
Pediatrics
Silvia Perin, Ivana Cataldo, Francesca Baciorri, Luisa Santoro, Angelo Paolo Dei Tos, Maria Guido, Paola Midrio
Summary: Fetal lung interstitial tumor (FLIT) is a rare pediatric lung tumor that can be mistaken for other lung neoplasms and congenital pulmonary malformations (cPAM) based on radiological features. A review of the literature found 21 cases of FLIT, with only 3 cases having a prenatal diagnosis. Surgery, usually lobectomy, was performed at an average age of 31 days, and there were no reported complications or disease recurrence during a mean follow-up of 49 months. Histology and immunohistochemistry can differentiate FLIT from other lung tumors with poor prognosis.
Letter
Cell Biology
Elena Bellan, Carlo Patriarca, Angelo P. Dei Tos, Marta Sbaraglia
Article
Cell Biology
Costantino Errani, Ilaria De Benedictis, Alberto Righi, Beatrice Valenti, Elisa Del Savio, Davide Baldazzi, Stefania Benini, Marta Sbaraglia, Brayan Vega Jimenez, Daniel Vanel, Davide Maria Donati, Angelo Paolo Dei Tos, Roberta Maestro
Summary: This retrospective study analyzed the clinical behavior and biology of epithelioid hemangioma (EH) of bone. The results showed that multifocal lesions of bone EH are clonally related, indicating the spread of a same neoplastic clone rather than simultaneous independent tumors. This finding contradicts the benign clinical course of EH and suggests that tumor dissemination in bone EH may be a phenomenon of passive spreading.
Article
Pathology
Albino Eccher, Angelo Paolo Dei Tos, Aldo Scarpa, Vincenzo L'Imperio, Enrico Munari, Giancarlo Troncone, Antonio Giuseppe Naccarato, Davide Seminati, Fabio Pagni
Summary: Despite reluctance to invest and challenges in estimating supporting costs, optimizing archives is becoming a hot topic in pathology laboratory management. Historically, archives were inadequate for securely storing and organizing tissue specimens, leading to risks for patients' health and legal consequences for pathologists.
JOURNAL OF CLINICAL PATHOLOGY
(2023)
Review
Oncology
J. -y. Blay, M. von Mehren, R. L. Jones, J. Martin-Broto, S. Stacchiotti, S. Bauer, H. Gelderblom, D. Orbach, N. Hindi, A. Dei Tos, M. Nathenson
Summary: Synovial sarcoma is a rare and aggressive disease, accounting for 5%-10% of all soft tissue sarcomas. Diagnosis is often challenging, but referral to a specialist center is crucial for optimal outcomes. Treatment strategies for localized disease include surgery, radiotherapy, and chemotherapy, while advanced disease has a poor prognosis and requires novel management strategies such as targeted agents and immunotherapies.