Article
Microbiology
Yafei Li, Wei Li, Yu Pan, Chang Liu, Siyu Liang, Zhenling Zeng
Summary: This study reveals a widespread presence of antimicrobial resistant livestock-associated MRSA (LA-MRSA) in animal farms and slaughterhouses in Chongqing, China. The MRSA isolates showed a high level of multidrug resistance, with the majority belonging to MLST type ST239 and carrying SCCmec types III, IVa, XII, and V.
VETERINARY MICROBIOLOGY
(2022)
Article
Infectious Diseases
Khuliso Ramaite, Mutshiene Deogratias Ekwanzala, John Barr Dewar, Maggy Ndombo Benteke Momba
Summary: Livestock environmental matrices may serve as reservoirs of MRSA, potentially disseminating through runoff to contaminate water resources. Continued surveillance of HA-MRSA in livestock environments is necessary.
Article
Genetics & Heredity
Hongbin Chen, Yuyao Yin, Lucy van Dorp, Liam P. Shaw, Hua Gao, Mislav Acman, Jizhen Yuan, Fengning Chen, Shijun Sun, Xiaojuan Wang, Shuguang Li, Yawei Zhang, Rhys A. Farrer, Hui Wang, Francois Balloux
Summary: The study reveals that in China, the community-associated MRSA strain ST59 is gradually replacing the healthcare-associated strain ST239, mainly due to its higher virulence potential rather than antibiotic resistance or biofilm production. The chemotaxis inhibitory protein (chp) carried by ST59 may be a significant factor contributing to its increased virulence.
Article
Microbiology
Abdeljallil Zeggay, Alban Atchon, Benoit Valot, Didier Hocquet, Xavier Bertrand, Kevin Bouiller
Summary: Using genomic data, the study determined the origin of MRSA ST398 isolates causing invasive infections in patients with no livestock contact. The isolates were sequenced and analyzed for the presence of virulence and resistance genes. Phylogenetic analysis revealed that MSSA isolates were human-associated, while MRSA isolates were livestock-associated. The results suggest that livestock-associated MRSA acquired virulence genes, enabling them to cause invasive infections in humans.
Article
Environmental Sciences
Anne Mette Madsen, Fei Zhang, Yonghui Zeng, Margit W. Frederiksen
Summary: This study investigated the potential human exposure to airborne microorganisms, including MRSA, in a pigeon exhibition in Denmark. The results showed high concentrations of fungi, bacteria, and endotoxin in the air, with concentrations up to 200 times higher than outdoor references. The bacteria found in the exhibition were mainly species found in pigeon coops, indicating that pigeons are the sources of exposure. The presence of airborne MRSA highlights the importance of considering this environment as a potential place of exchange of resistant bacteria between animals and humans.
ENVIRONMENTAL RESEARCH
(2023)
Article
Infectious Diseases
Majda Golob, Mateja Pate, Darja Kusar, Urska Zajc, Bojan Papi, Matjaz Ocepek, Irena Zdovc, Jana Avbersek
Summary: Pigs were identified as the primary reservoir of livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA), with farm size and management practices affecting the prevalence and characteristics of MRSA.
Article
Microbiology
Vanessa Silva, Ana Oliveira, Vera Manageiro, Manuela Canica, Diogo Contente, Rosa Capita, Carlos Alonso-Calleja, Isabel Carvalho, Jose L. Capelo, Gilberto Igrejas, Patricia Poeta
Summary: The study aimed to characterize the antimicrobial resistance and clonal lineages of MRSP isolated from canine cutaneous pyoderma. The majority of isolates belonged to ST123 and exhibited multidrug resistance, posing a significant threat to animal health.
Review
Environmental Sciences
Ying Wang, Peihua Zhang, Jian Wu, Shuaiyin Chen, Yuefei Jin, Jinzhao Long, Guangcai Duan, Haiyan Yang
Summary: Staphylococcus aureus (S. aureus) is a dangerous bacterial pathogen that can infect both humans and animals. Methicillin-resistant S. aureus (MRSA) is classified into different types based on their sources, such as hospital-associated (HA-MRSA), community-associated (CA-MRSA), and livestock-associated MRSA (LA-MRSA). LA-MRSA, initially associated with livestock, has spread among humans, animals, and the environment due to factors like animal husbandry, globalization, and antibiotic use. This review provides an overview of the transmission characteristics of S. aureus and its clones in different hosts, as well as the changes in mobile genetic elements (MGEs) during host switching.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Medicine, General & Internal
Ching Hoong Chew, Chew Chieng Yeo, Ainal Mardziah Che Hamzah, Esra'a I. Al-Trad, Sherry Usun Jones, Kek Heng Chua, Suat Moi Puah
Summary: Multidrug resistance (MDR) poses a significant challenge in healthcare management, and a comprehensive approach is needed to address this issue. In this study, a combination of phenotypic and genotypic methods, including whole genome sequencing (WGS), was used to investigate hospital-associated MDR methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from newborn infants. The findings revealed different levels of resistance to antimicrobial classes among the MDR-MRSA strains, along with the presence of slime and biofilms, SCCmec type IV, and various types of microbial surface components recognizing adhesive matrix molecules (MSCRAMMs) and virulence genes. The WGS data for specific strains showed the presence of antimicrobial resistance genes in the chromosomes, with one gene found in a small plasmid. The presence of MDR-MRSA strains in neonates raises public concern, and multifaceted interventions are recommended to address this issue. Furthermore, there is a need for metadata to improve the investigation of antimicrobial resistance genes in MDR isolates.
Article
Public, Environmental & Occupational Health
R. P. Smith, M. Sharma, D. Gilson, M. Anjum, C. J. Teale
Summary: This study investigated the occurrence of LA-MRSA in pigs at slaughter, with 43.8% of batches testing positive for MRSA. The proportion of positive batches varied among abattoirs, indicating possible cross-contamination between lairage and post-stunning. One abattoir consistently provided negative samples, suggesting differences in pig MRSA status upon arrival from supply farms.
EPIDEMIOLOGY AND INFECTION
(2021)
Article
Multidisciplinary Sciences
Gopalan Krishnan Sivaraman, Visnuvinayagam Sivam, Balasubramanian Ganesh, Ravikrishnan Elangovan, Ardhra Vijayan, Mukteswar Prasad Mothadaka
Summary: The study reported the draft genome sequence of a unique ST28/t021 CA-MRSA strain isolated from ribbon fish, which is the first report from India. In addition to antibiotic resistance, this strain also harbors various virulence genes.
Article
Biochemistry & Molecular Biology
In-Geun Jung, Jae-Young Jeong, Seung-Hoon Yum, You-Jin Hwang
Summary: This study screened 16 medicinal plants and found that some extracts and fractions showed significant inhibitory effects on Staphylococcus aureus, including multidrug-resistant strains, with low cytotoxicity to human cells.
Article
Biology
Ehssan H. Moglad, Hisham N. Altayb
Summary: This study investigates the resistance patterns of Staphylococcus spp. isolated from clinical samples in Khartoum State, Sudan, revealing a high prevalence of Methicillin-resistant Staphylococcus aureus (MRSA). The study highlights the need for effective antimicrobial stewardship programs to reduce unnecessary antibiotic costs.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Preeyaporn M. Sreepian, Panthip Rattanasinganchan, Apichai Sreepian
Summary: Citrus hystrix essential oil (CHEO) has been found to have broad-spectrum antibacterial activity and can be used in combination with gentamicin to inhibit the growth of methicillin-resistant S. aureus (MRSA) and reduce the emergence of resistant bacteria.
SAUDI PHARMACEUTICAL JOURNAL
(2023)
Article
Infectious Diseases
Surawit Chueahiran, Jitrapa Yindee, Pongthai Boonkham, Nipattra Suanpairintr, Pattrarat Chanchaithong
Summary: This study aimed to investigate the molecular and antimicrobial resistance characteristics of MRSA CC398 isolated from diseased dogs and cats in Thailand. The results showed that these isolates mainly carried resistance genes to beta-lactams, tetracyclines, aminoglycosides, and trimethoprim, and displayed intraclonal differentiation. The presence of CC398 MRSA with intraclonal variation suggests that CC398 is a successful MRSA clone colonizing in small animals as a pet-associated MRSA in Thailand.
Article
Infectious Diseases
Ana Sofia R. Duarte, Ana Rita Marques, Vibe D. Andersen, Helle B. Korsgaard, Hanne Mordhorst, Frederik D. Moller, Thomas N. Petersen, Tine Hald, Frank M. Aarestrup
Summary: This study aimed to evaluate the relevance of indicator bacteria (Escherichia coli and Enterococcus faecalis) for AMR surveillance in pigs, and the utility of metagenomics. The results showed that metagenomics is a promising approach for AMR surveillance, as it allows for ranking of resistance genes and correlation analysis with antimicrobial use.
Article
Environmental Sciences
Ray W. Izquierdo-Lara, Leo Heijnen, Bas B. Oude Munnink, Claudia M. E. Schapendonk, Goffe Elsinga, Jeroen Langeveld, Johan Post, Divyae K. Prasad, Christian Carrizosa, Frederic Been, Janko van Beek, Remy Schilperoort, Rianne Vriend, Ewout Fanoy, Evelien I. T. de Schepper, Reina S. Sikkema, Richard Molenkamp, Frank M. Aarestrup, Gertjan Medema, Marion P. G. Koopmans, Miranda de Graaf
Summary: Monitoring SARS-CoV-2 in wastewater has the potential to provide valuable information about viral dynamics and diversity within a population. In this study, sewage samples from different areas in Rotterdam were sequenced, and specific signature mutations were used to estimate the relative abundances of SARS-CoV-2 lineages. The results were compared with clinical genomic surveillance data to track the emergence, dominance, and replacement of specific variants of concern (VOCs). The study also demonstrated the ability to detect spatio-temporal clusters and identify mutations not captured by clinical surveillance.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Microbiology
Helle Brander Eriksen, Kurt Fuursted, Anders Jensen, Christian Salgard Jensen, Xiaohui Nielsen, Jens Jorgen Christensen, Patricia Shewmaker, Ana Rita Rebelo, Frank Moller Aarestrup, Kristian Schonning, Hans-Christian Slotved
Summary: The susceptibility of Streptococcus pneumoniae to beta-lactam drugs can be predicted from the amino acid sequence of penicillin-binding proteins (PBPs). A BLAST analysis can accurately predict the susceptibility of Danish S. pneumoniae isolates and some internationally collected MGS isolates. However, the current database is not effective in predicting the susceptibility of other MGS strains.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Infectious Diseases
Manuela Iurescia, Elena Lavinia Diaconu, Patricia Alba, Fabiola Feltrin, Carmela Buccella, Roberta Onorati, Angelo Giacomi, Andrea Caprioli, Alessia Franco, Antonio Battisti, Virginia Carfora
Summary: This study investigated the genomic characteristics of cfr-positive, multidrug-resistant livestock-associated MRSA in Italian pig holdings. The results showed that the isolates had different cfr variants, with one displaying linezolid resistance. The cfr gene was found on a novel plasmid in the 2008 CC1 isolate, while it was located on the chromosome in the 2010 CC398 isolate.
Article
Infectious Diseases
Pimlapas Leekitcharoenphon, Hakan Vigre, Rolf Sommer Kaas, Frank M. Aarestrup
Summary: This study analyzed 421 Salmonella Dublin genomes from cattle and food in Denmark to determine the trend of the bacterial population and the impact of intervention. The results showed a decrease in the population size of Salmonella Dublin in both cattle and humans, indicating the effectiveness of surveillance programs. Whole genome sequencing combined with phylogenetic analysis is an important tool for assessing the impact of control measures on bacterial population and human infection risk.
INFECTION GENETICS AND EVOLUTION
(2023)
Article
Multidisciplinary Sciences
Markus H. K. Johansson, Frank M. Aarestrup, Thomas N. Petersen
Summary: This study investigates the geographic distribution and global dissemination of antimicrobial resistance genes (ARGs) facilitated by mobile genetic elements (MGEs) through the analysis of 677 metagenomic sequenced sewage samples from 97 countries or regions. The study finds systematic differences in the abundance of MGEs and ARGs, with some elements prevalent worldwide while others show higher abundance in specific geographic areas. Climate is identified as an important factor influencing the local distribution of MGEs.
Article
Multidisciplinary Sciences
Sidsel Nag, Gunhild Larsen, Judit Szarvas, Laura Elmlund Kohl Birkedahl, Gabor Mate Gulyas, Wojchiech Jakub Ciok, Timmie Mikkel Lagermann, Silva Tafaj, Susan Bradbury, Peter Collignon, Denise Daley, Victorien Dougnon, Kafayath Fabiyi, Boubacar Coulibaly, Rene Dembele, Georgette Nikiema, Natama Magloire, Isidore Juste Ouindgueta, Zenat Zebin Hossain, Anowara Begum, Deyan Donchev, Mathew Diggle, Leeann Turnbull, Simon Levesque, Livia Berlinger, Kirstine Kobberoe Sogaard, Paula Diaz Guevara, Carolina Duarte Valderrama, Panagiota Maikanti, Jana Amlerova, Pavel Drevinek, Jan Tkadlec, Milica Dilas, Achim Kaasch, Henrik Torkil Westh, Mohamed Azzedine Bachtarzi, Wahiba Amhis, Carolina Elisabeth Satan Salazar, JoseEduardo Villacis, Maria Angeles Dominguez Luzon, Damaris Berbel Palau, Claire Duployez, Maxime Paluche, Solomon Asante-Sefa, Mie Moller, Margaret Ip, Ivana Marekovic, Agnes Pal-Sonnevend, Clementiza Elvezia Cocuzza, Asta Dambrauskiene, Alexandre Macanze, Anelsio Cossa, Inacio Mandomando, Philip Nwajiobi-Princewill, Iruka N. Okeke, Aderemi O. Kehinde, Ini Adebiyi, Ifeoluwa Akintayo, Oluwafemi Popoola, Anthony Onipede, Anita Blomfeldt, Nora Elisabeth Nyquist, Kiri Bocker, James Ussher, Amjad Ali, Nimat Ullah, Habibullah Khan, Natalie Weiler Gustafson, Ikhlas Jarrar, Arif Al-Hamad, Viravarn Luvira, Wantana Paveenkittiporn, Irmak Baran, James C. L. Mwansa, Linda Sikakwa, Kaunda Yamba, Rene Sjogren Hendriksen, Frank Moller Aarestrup
Summary: The Two Weeks in the World research project has gathered a dataset of 3087 clinically relevant bacterial genomes with corresponding metadata, collected from 59 diagnostic units in 35 countries worldwide in 2020. A relational database is available, providing metadata and summary data from selected bioinformatic analysis, such as species prediction and identification of acquired resistance genes.
Article
Microbiology
Emilie Egholm Bruun Jensen, Victoria Sedor, Emmanuel Eshun, Patrick Njage, Saria Otani, Frank M. Aarestrup
Summary: Limited knowledge and research exist on the bacteriome and resistome in livestock in Africa. This study compared the antimicrobial resistance gene (ARG) pools and bacteriome in pigs and poultry from Ghana and European countries. Significant differences were found in ARG and bacterial abundance and composition between Ghanaian and European samples. The resistome composition also varied, with different resistance genes being more abundant in Ghanaian or European samples. This is the first report on the resistome in livestock from Sub-Saharan Africa using metagenomics.
Article
Environmental Sciences
Hannah C. Lepper, Meghan R. Perry, Bryan A. Wee, David Wills, Hanne Nielsen, Saria Otani, Moray Simon, Frank M. Aarestrup, Mark E. J. Woolhouse, Bram A. D. van Bunnik
Summary: This study compares the resistome and microbiome of hospital, community, and mixed municipal wastewater and finds that the resistome in hospital wastewater is different from that in other locations.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Cell Biology
Omid Pajand, Hamzeh Rahimi, Farzad Badmasti, Faeze Gholami, Tahereh Alipour, Narges Darabi, Frank M. Aarestrup, Pimlapas Leekitcharoenphon
Summary: In this study, the transmission of bla(NDM-1) in K. pneumoniae in Iran was found to occur both clonally and horizontally via various combinations of mobile genetic elements (MGEs), with ST147 being the major clone associated with bla(NDM-1) dissemination. This is the first analysis of the Iranian ST147/NDM+ clone in the global context.
JOURNAL OF BIOMEDICAL SCIENCE
(2023)
Article
Microbiology
Patricia Alba, Virginia Carfora, Fabiola Feltrin, Elena Lavinia Diaconu, Luigi Sorbara, Elena Dell'Aira, Tamara Cerci, Angela Ianzano, Valentina Donati, Alessia Franco, Antonio Battisti
Summary: The increasing prevalence of multidrug-resistant S. Infantis carrying pESI(like) megaplasmid in Europe is a major concern. This study sequenced and analyzed five prototype pESI(like) plasmids and found that although they have almost identical sequences, their structures vary, indicating different insertion or transposition events. These findings are essential for understanding the plasticity and evolution of pESI(like) megaplasmid and can contribute to risk management and policy decisions in combating antimicrobial resistance and multidrug resistance in food-borne pathogens.
FEMS MICROBIOLOGY LETTERS
(2023)
Article
Environmental Sciences
Patrick Murigu Kamau Njage, Bram Van Bunnik, Patrick Munk, Ana Rita Pinheiro Marques, Frank M. Aarestrup
Summary: This study used metagenomic analysis of sewage samples to monitor and predict antimicrobial resistance. The findings showed a strong association between bacterial groups in the human gut and antimicrobial resistance. Additionally, there were variations in the associations between socioeconomic, nutritional, and health factors and antimicrobial resistance across different countries and antimicrobial classes.
LANCET PLANETARY HEALTH
(2023)
Article
Fisheries
Ana Isabel Vela, Maria del Mar Blanco, Silvia Colussi, Charalampos Kotzamanidis, Marino Prearo, Ilhan Altinok, Pier Luigi Acutis, Donatella Volpatti, Patricia Alba, Fabiola Feltrin, Angela Ianzano, Lucas Dominguez, Jose Francisco Fernandez-Garayzabal
Summary: Lactococcosis is a globally prevalent infectious disease that has a significant economic and sanitary impact on the rainbow trout industry. This study reveals that both Lactococcus garvieae and Lactococcus petauri are implicated in the etiology of lactococcosis, and that L. petauri plays a significant role in the epidemiology of this disease. Additionally, some biochemical differences between the two species may serve as phenotypic markers for their presumptive recognition.
