Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 81, Issue 21, Pages 7525-7532Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.01690-15
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Funding
- NASA Astrobiology: Exobiology and Evolutionary Biology program [NNX08AO15G]
- NASA Earth and Space Science Fellowship (NESSF) Program [NNX13AP74H]
- National Science Foundation [1349029]
- NASA [467477, NNX08AO15G, 95807, NNX13AP74H] Funding Source: Federal RePORTER
- Direct For Biological Sciences
- Div Of Biological Infrastructure [1349029] Funding Source: National Science Foundation
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Knowledge of how microorganisms respond and adapt to low-pressure (LP) environments is limited. Previously, Bacillus subtilis strain WN624 was grown at the near-inhibitory LP of 5 kPa for 1,000 generations and strain WN1106, which exhibited increased relative fitness at 5 kPa, was isolated. Genomic sequence differences between ancestral strain WN624 and LP-evolved strain WN1106 were identified using whole-genome sequencing. LP-evolved strain WN1106 carried amino acid- altering mutations in the coding sequences of only seven genes (fliI, parC, ytoI, bacD, resD, walK, and yvlD) and a single 9-nucleotide in-frame deletion in the rnjB gene that encodes RNase J2, a component of the RNA degradosome. By using a collection of frozen stocks of the LP-evolved culture taken at 50-generation intervals, it was determined that (i) the fitness increase at LP occurred rapidly, while (ii) mutation acquisition exhibited complex kinetics. A knockout mutant of rnjB was shown to increase the competitive fitness of B. subtilis at both LP and standard atmospheric pressure.
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