Article
Infectious Diseases
Xiaodong Wang, Paride Abliz, Shuwen Deng
Summary: This study investigated macrolide resistance in Treponema pallidum from patients with latent syphilis in Xinjiang, China. The results indicated the presence of macrolide resistance in T. pallidum in Xinjiang, with A2058G being the predominant resistance mechanism. Blood samples may serve as suitable specimens for detecting resistant mutations in T. pallidum in patients with latent syphilis, even without clinical symptoms.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Microbiology
P. A. Grange, A. Jary, C. Isnard, S. Burrel, D. Boutolleau, A. Touati, C. Bebear, J. Saule, P. Martinet, J-L Robert, D. Moulene, A. Vermersch-Langlin, N. Benhaddou, M. Janier, N. Dupin
Summary: The commercial Allplex genital ulcer realtime PCR multiplex assay was evaluated for detecting Treponema pallidum and other pathogens. The study concluded that this assay is suitable for routine detection of T. pallidum subsp. pallidum and enables rapid simultaneous detection of multiple pathogens.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Immunology
Cuini Wang, Zhixiang Hu, Xin Zheng, Meiping Ye, Chunjie Liao, Mengya Shang, Weiming Gong, Zhifang Guan, Haikong Lu, Xin Gu, Mei Shi, Pingyu Zhou
Summary: The study revealed significant differences in the detection rate and quantity of Treponema pallidum DNA in saliva among patients with different stages of syphilis. The load of T. pallidum DNA in saliva decreases after treatment, indicating its potential as an indicator of therapeutic effectiveness.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Microbiology
Cuini Wang, Xin Zheng, Zhifang Guan, Danyang Zou, Xin Gu, Haikong Lu, Mei Shi, Pingyu Zhou
Summary: This study demonstrated that Treponema pallidum DNA can be detected in urine in patients at all stages of syphilis, with significantly higher detection rates and loads in urine sediment compared to urine supernatant. Therefore, urine may serve as an ideal sample for acquiring a large amount of T. pallidum DNA for supplemental detection in cases difficult to diagnose.
MICROBIOLOGY SPECTRUM
(2022)
Review
Infectious Diseases
Qian Zhou, Jingwei Liu, Shaochun Chen, Wenqi Xu, Yan Han, Yueping Yin
Summary: The meta-analysis shows that the C2611T mutation in the 23S rRNA of N. gonorrhoeae is valuable for molecular diagnostics of moderate-level azithromycin resistance, particularly when the moderate level is set at 4-128 mg/L. This rapid molecular detection method can be used for rapid identification of drug-resistant isolates in clinical settings.
Article
Multidisciplinary Sciences
Gheyath K. Nasrallah, Raniya Al-Buainain, Nadin Younes, Soha R. Dargham, Duaa W. Al-Sadeq, Mohamed Elhassan, Ibrahim Al-Shaar, Hadi M. Yassine, Laith J. Abu-Raddad, Mohamed M. Emara, Ahmed Ismail
Summary: HIV and Syphilis are common STIs that pose a concern and burden on healthcare systems. This study evaluated the diagnostic efficiency of the protocols used by the Medical Commission Department (MC) in Qatar for screening these infections.
Article
Food Science & Technology
M. A. Motalib Hossain, Siti Aimi Sarah Zainal Abidin, Aishah Bujang, Mohd Nasir Taib, Suresh Sagadevan, Mohd Rafie Johan, Nina Naquiah Ahmad Nizar
Summary: Meat and meat products are highly sought after globally for their high-quality protein and nutrients, but the industry faces challenges such as food fraud, mislabeling, and zoonotic diseases. Quantitative detection of the species origin using methods like PCR is crucial for food control. This review focuses on the design, development, and applications of TaqMan probe-based mqPCR techniques for authenticating meat products, providing a reference for academia and industry to improve existing assays.
Article
Microbiology
Cau D. Pham, Evelyn Nash, Hsi Liu, Matthew W. Schmerer, Samera Sharpe, Grace Woods, Brad Roland, Karen Schlanger, Sancta B. St Cyr, Jonathan Carlson, Kevin Sellers, Aaron Olsen, Ruth Sanon, Henrietta Hardin, Olusegun O. Soge, Brian H. Raphael, Ellen N. Kersh
Summary: A mutation in the 23S rRNA gene at position A2059G is known to confer high-level azithromycin resistance in Neisseria gonorrhoeae. However, through surveillance projects in the U.S., four isolates lacking this mutation were found to have a different mutation at position A2058G. In vitro experiments confirmed that this A2058G mutation can also confer high-level azithromycin resistance in N. gonorrhoeae.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Microbiology
Jacky Flipse, Anne-Marie Niekamp, Anne Dirks, Nicole H. T. M. Dukers-Muijrers, Christian J. P. A. Hoebe, Petra Wolffs, Inge H. M. van Loo
Summary: Treponema pallidum subsp. pallidum is the causative agent of syphilis, and its diagnosis and disease staging are based on clinical findings and serologic testing. PCR analysis of swab samples from genital ulcers is recommended, but its added value is debatable. In this study, both PCR and IgM serology were found to aid in the diagnosis of early syphilis.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Infectious Diseases
Liufeng Yuan, Deju Xia, Qian Zhou, Wenqi Xu, Sihong Xu, Yueping Yin
Summary: Multiplex PCR is a cost-effective method that can be used for the detection of Treponema pallidum, herpes simplex virus type 1 and 2 (HSV-1,2) using limited clinical material. The study established a multiplex TP-HSV1-HSV2 Polymerase Chain Reaction (multiplex PCR) to test skin lesions of 115 patients suspected of having TP and HSV1/2 infections. The method showed high clinical sensitivity and specificity for all three pathogens and is particularly useful for early TP infection and differential diagnosis of new skin lesions in patients with a history of previous syphilis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2023)
Article
Biochemistry & Molecular Biology
Ivica Samanic, Blanka Dadic, Zeljka Sanader Marsic, Mia Dzelalija, Ana Maravic, Hrvoje Kalinic, Pavle Vrebalov Cindro, Zeljko Sundov, Marija Tonkic, Ante Tonkic, Jonatan Vukovic
Summary: This study investigated the molecular genetic mechanisms of clarithromycin and levofloxacin resistance in Helicobacter pylori infections in patients from Croatia. Point mutations in the 23S rRNA, gyrA, and gyrB genes were found to alter target sites or protein structure, leading to reduced efficacy of standard antibiotics. Molecular docking analysis revealed that resistance-related mutations resulted in decreased susceptibility to clarithromycin and levofloxacin due to significant differences in non-covalent interactions, ultimately resulting in antibiotic resistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Krishnamoorthy Priya, Pasupathi Rathinasabapathi, Rex Arunraj, Dhanasekaran Sugapriya, Mohandass Ramya
Summary: This study developed a multiplex HRM-LAMP assay targeting the marker genes polA and tprL to detect Treponema pallidum. The developed method showed high sensitivity and specificity in detecting syphilis, providing a rapid and accurate diagnostic approach.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Immunology
Wenyin He, Ying Yuan, Junyu Liang, Xuejiao Fan, Lei Li, Xingfei Pan
Summary: This study developed a nested real-time PCR method for simultaneous detection of 23S rRNA and parC genotypes in Mycoplasma genitalium, which can be used to determine macrolide and fluoroquinolone resistance. The method showed high sensitivity, rapidity, and ease of interpretation.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Dermatology
Carolina Labrandero Hoyos, Rodrigo Penuelas Leal, Andres Casanova Esquembre, Javier Lorca Sproehnle, Andres Grau Echevarria, Jorge Magdaleno Tapial, Alvaro Martinez-Domenech, Maria Dolores Ocete Mochon, Pablo Hernandez Bel
Summary: This study aimed to analyze the sensitivity of Treponema pallidum detection by polymerase chain reaction (PCR) in patients diagnosed with syphilis. The results showed that the sensitivity was highest (95.2%) when performed on samples from mucocutaneous ulcers in primary syphilis. However, the sensitivity decreased to 69.4% in secondary syphilis.
AUSTRALASIAN JOURNAL OF DERMATOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Svetlana N. Kovalchuk, Anna L. Arkhipova
Summary: This article describes the development of a TaqMan PCR assay for the detection of A and B variants of bovine BLG. Compared to the commonly used PCR-RFLP, this one-step method requires less time and is more suitable for large-scale genotyping of cattle.
ANIMAL BIOTECHNOLOGY
(2022)