Comparison of Medium, Temperature, and Length of Incubation for Detection of Vancomycin-Resistant Enterococcus

Campylobacter (Campy; BD Diagnostics, Sparks, MD), Spectra VRE (Remel, Lenexa, KS), and bile-esculin-azide-vancomycin (BEAV; Remel) agars were compared for their ability to detect vancomycin-resistant enterococci (VRE) in 750 stool specimens. The media were compared at 24 h and 48 h of incubation at 35 degrees C and 42 degrees C. When incubated for 24 h at 35 degrees C, Campy was the most sensitive (97.8%) and specific (99.9%) but was comparable to Spectra, which has a sensitivity of 95.6% and a specificity of 99.1%, whereas BEAV was significantly less sensitive (90%) and specific (96.1%). Incubation at 42 degrees C or extended incubation at 35 degrees C for 48 h yielded no advantage over incubation at 35 degrees C for 24 h.