Article
Microbiology
Sarita Manandhar, Anjana Singh, Ajit Varma, Shanti Pandey, Neeraj Shrivastava
Summary: The study reveals a high prevalence of biofilm formation and inducible clindamycin resistance in CNS isolates, emphasizing the importance of in-vitro biofilm production test and D-test in routine laboratory diagnostics.
ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS
(2021)
Article
Infectious Diseases
Fei Jiang, Ziyan Kong, Ke Liu, Chen Cheng, Tingting Jiang, Ping Ma, Rongpeng Li
Summary: This study characterized linezolid-resistant coagulase-negative staphylococci (CoNS) isolates in China and assessed the possibility of linezolid dependence. The results showed high-level linezolid resistance in CoNS isolates, but no linezolid-dependent growth was observed under linezolid selective pressure. These findings are important for understanding and controlling the dissemination of CoNS.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2022)
Article
Multidisciplinary Sciences
Tanshi Mehrotra, Dipasri Konar, Agila Kumari Pragasam, Shakti Kumar, Pradipta Jana, Prabhakar Babele, Deepjyoti Paul, Ayushi Purohit, Subhash Tanwar, Susmita Bakshi, Santanu Das, Jyoti Verma, Daizee Talukdar, Lekshmi Narendrakumar, Akanksha Kothidar, Sonali Porey Karmakar, Susmita Chaudhuri, Sujoy Pal, Kajal Jain, Chittur V. Srikanth, M. Jeeva Sankar, Krishnamohan Atmakuri, Ramesh Agarwal, Rajni Gaind, Mamatha Ballal, Nagamani Kammili, Rupak K. Bhadra, Thandavarayan Ramamurthy, G. Balakrish Nair, Bhabatosh Das
Summary: Microbes evolve rapidly through mutations and acquisition of mobile genetic elements (MGEs) associated with fitness traits like antimicrobial resistance (AMR). A multicentric study in India decoded genome sequences of bacterial pathogens causing sepsis, urinary tract infections, and respiratory infections. The study identified pathogen-specific AMR genes (ARGs) and MGEs, and differential abundance of resistance-associated proteins in the presence and absence of antibiotics, providing valuable insights for AMR diagnostics and antimicrobial therapy.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Genetics & Heredity
Jonathan Asante, Akebe L. K. Abia, Daniel Anokwah, Bakoena A. Hetsa, Dorcas O. Fatoba, Linda A. Bester, Daniel G. Amoako
Summary: The study investigates biofilm formation and biofilm/adhesion-encoding genes in coagulase-negative staphylococci (CoNS) recovered from blood culture isolates. A total of 89 clinical CoNS were confirmed using the VITEK 2 system and qualitative and quantitative assays were performed to assess biofilm formation. The presence of adherence-related genes was also investigated, and a high prevalence of biofilm-forming CoNS with various resistance phenotypes was observed.
Article
Microbiology
Vanessa Silva, Elisete Correia, Jose Eduardo Pereira, Camino Gonzalez-Machado, Rosa Capita, Carlos Alonso-Calleja, Gilberto Igrejas, Patricia Poeta
Summary: This study evaluates the ability of biofilm formation in coagulase-negative staphylococci (CoNS) and S. pseudintermedius isolated from various animal species, and investigates the effect of conventional antimicrobials on reducing biofilm. The results show that all isolates can form biofilm, with S. urealyticus producing the most biomass and S. pseudintermedius producing the least biomass. Biofilm formation is positively associated with multidrug resistance and resistance to individual antimicrobials. Tetracycline and amikacin were unable to eradicate the biofilm, even at high concentrations.
Article
Chemistry, Applied
Li Yang, Hongjun Li, Han Wu, Shuyun Liu, Chang Su, Zhifei He
Summary: This study isolated and identified Coagulase-negative Staphylococci (CNS) from Chinese bacon, and evaluated their ability to degrade proteins and improve the taste of the bacon. Two CNS species were found to be capable of degrading proteins and enhancing the taste of Chinese bacon.
Review
Microbiology
Angela Franca, Vania Gaio, Nathalie Lopes, Luis D. R. Melo
Summary: Coagulase-negative staphylococci (CoNS) have become major pathogens in healthcare-associated facilities, with a focus on biofilm formation and antibiotic resistance genes. These factors make treatment challenging, despite their lower virulence compared to S. aureus.
Article
Food Science & Technology
Wioleta Chajecka-Wierzchowska, Joanna Gajewska, Anna Zadernowska, Cinzia Lucia Randazzo, Cinzia Caggia
Summary: The present study aimed to evaluate and describe the diversity of CoNS strains as potential vectors for the transmission of antimicrobial resistance from RTE foods served in bars and restaurants. The study found that out of 85 CoNS strains, 67 strains were resistant to at least one antibiotic, with 37 strains exhibiting multidrug resistance (MDR-CoNS). Furthermore, CoNS strains carried resistance genes for antibiotics critically important in medicine, such as beta-lactams, aminoglycosides, and tetracyclines. The high frequency of MDR-CoNS in RTE foods poses a direct risk to public health as it contributes to the gene pool for acquisition of resistance traits by pathogenic bacteria.
Article
Microbiology
Vladimir Gostev, Semen Leyn, Alexander Kruglov, Daria Likholetova, Olga Kalinogorskaya, Marina Baykina, Natalia Dmitrieva, Zlata Grigorievskaya, Tatiana Priputnevich, Lyudmila Lyubasovskaya, Alexey Gordeev, Sergey Sidorenko
Summary: Coagulase-negative staphylococci (CoNS) were historically considered non-pathogenic, but strains such as S. epidermidis, S. haemolyticus, and S. hominis have now been identified as opportunistic pathogens. The off-label use of linezolid for CoNS infections has led to the emergence of international linezolid-resistant lineages. Genetic analysis revealed the presence of common ancestors for linezolid-resistant strains in different countries.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Luiza Pinheiro-Hubinger, Danilo Flavio Moraes Riboli, Ligia Maria Abraao, Eliane Patricia Lino Pereira Franchi, Maria de Lourdes Ribeiro de Souza da Cunha
Summary: This study compared the clonality and SCCmec type of CoNS isolates from different sources in the same community, revealing high clonal diversity in S. epidermidis and circulation of some S. haemolyticus clones in both hospital and community. The presence of different SCCmec types within the same cluster indicated high diversity, suggesting SCCmec may not be a good marker of clonality in these bacteria.
Article
Veterinary Sciences
Yu Jin Lee, Young Ju Lee
Summary: This study investigated the pathogenic potential of Coagulase-negative staphylococci (CoNS) isolated from bulk tank milk. The results showed that these bacteria exhibited a high prevalence of biofilm-forming ability and multidrug resistance. Therefore, developing a strong monitoring and sanitation program for dairy factories is crucial for ensuring hygienic milk production.
VETERINARY SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Federica Fogacci, Valentina Di Micoli, Ashot Avagimyan, Marina Giovannini, Egidio Imbalzano, Arrigo F. G. Cicero
Summary: This review aims to discuss the best strategy for utilizing molecular biology techniques to examine the genetic role of Lp(a) and its significance in clinical research and practice. The diversity in size of apo(a) is primarily caused by copy number variations in the KIV2 domains.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Ingrid Nayara Marcelino Santos, Mariana Neri Lucas Kurihara, Fernanda Fernandes Santos, Tiago Barcelos Valiatti, Juliana Thalita Paulino da Silva, Antonio Carlos Campos Pignatari, Mauro Jose Salles
Summary: This study describes the phenotypic and genomic features of Staphylococcus aureus and coagulase-negative Staphylococcus (CoNS) isolates causing orthopedic implant-associated infections (OIAIs). It was found that MRSA and CoNS isolates carrying genes for biofilm production and multiple resistance genes are associated with treatment failure in OIAIs.
Article
Infectious Diseases
Yahya Mohzari, Fahad Aljobair, Ahmed Alrashed, Syed Mohammed Basheeruddin Asdaq, Renad Abdullah Alshuraim, Suzan Suhail Asfour, Mountasser Mohammad Al-Mouqdad, Reem F. Bamogaddam, Deemah Al-Anazi, Catherine E. Zeilinger, Ahmad Alamer, Batool Mohammed Alhassan, Nagaraja Sreeharsha
Summary: In recent years, there has been an increase in the prevalence of gram-positive bacteremia in neonates, but better care has also led to improved survival rates for premature neonates. Coagulase-negative staphylococci (CoNS) is the most common pathogen, and daptomycin has shown to be effective in treating it. The study demonstrates that daptomycin has a higher clinical cure rate in neonates with gram-positive infections.
Article
Microbiology
Matthijs S. Berends, Christian F. Luz, Alewijn Ott, Gunnar Andriesse, Karsten Becker, Corinna Glasner, Alex W. Friedrich
Summary: This retrospective study provides an in-depth data analysis of CoNS isolates found in human blood culture isolates in the Northern Netherlands between 2013 and 2019. The study identifies 27 different CoNS species and observes significant differences in occurrence and antibiotic resistance profiles. However, these differences are often neglected in national guidelines.
Article
Microbiology
Christian J. H. von Wintersdorff, Petra F. G. Wolffs, Paul H. M. Savelkoul, Rianne R. R. Nijsen, Susanne Lau, Kerstin Gerhold, Eckard Hamelmann, John Penders
FUTURE MICROBIOLOGY
(2016)
Article
Infectious Diseases
Christian J. H. von Wintersdorff, Petra F. G. Wolffs, Julius M. van Niekerk, Erik Beuken, Lieke B. van Alphen, Ellen E. Stobberingh, Astrid M. L. Oude Lashof, Christian J. P. A. Hoebe, Paul H. M. Savelkoul, John Penders
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2016)
Article
Infectious Diseases
Petra J. Woestenberg, Audrey J. King, Marianne A. B. van der Sande, Robine Donken, Suzan Leussink, Fiona R. M. van der Klis, Christian J. P. A. Hoebe, Johannes A. Bogaards, Birgit H. B. van Benthem
JOURNAL OF INFECTION
(2017)
Article
Immunology
Petra J. Woestenberg, Audrey J. King, Birgit H. B. van Benthem, Robine Donken, Suzan Leussink, Fiona R. M. van der Klis, Hester E. de Melker, Marianne A. B. van der Sande, Christian J. P. A. Hoebe, Johannes A. Bogaards
JOURNAL OF INFECTIOUS DISEASES
(2018)
Article
Otorhinolaryngology
Darius Henatsch, Cindy H. Nabuurs, Rens M. van de Goor, Petra F. Wolffs, Robert J. Stokroos
OTOLARYNGOLOGY-HEAD AND NECK SURGERY
(2017)
Review
Pathology
Kevin J. H. Janssen, Jeanne A. M. C. Dirks, Nicole H. T. M. Dukers-Muijrers, Christian J. P. A. Hoebe, Petra F. G. Wolffs
EXPERT REVIEW OF MOLECULAR DIAGNOSTICS
(2018)
Article
Multidisciplinary Sciences
Julien N. A. P. Wijers, Genevieve A. F. S. van Liere, Christian J. P. A. Hoebe, Jochen W. L. Cals, Petra F. G. Wolffs, Nicole H. T. M. Dukers-Muijrers
Article
Clinical Neurology
Youssef Yakkioui, Ernst-Jan M. Speel, Jacobus J. Van Overbeeke, Merel J. M. Boderie, Sreedhar Pujari, Axel Zur Hausen, Petra F. G. Wolffs, Yasin Temel
WORLD NEUROSURGERY
(2018)
Article
Multidisciplinary Sciences
J. A. M. C. Dirks, K. Janssen, C. J. P. A. Hoebe, T. H. B. Geelen, M. Lucchesi, N. H. T. M. Dukers-Muijrers, P. F. G. Wolffs
Summary: The total plasmid copy numbers in clinical urogenital samples were found to be highly variable, while intra-bacterial plasmid copy numbers were more stable. DNA degradation in urine was influenced by transport time and temperature more significantly, supporting the use of a plasmid target in Chlamydia trachomatis screening assays to increase clinical sensitivity.
SCIENTIFIC REPORTS
(2021)
Article
Gastroenterology & Hepatology
Matthew Davies, Gianluca Galazzo, Jarne M. van Hattem, Maris S. Arcilla, Damian C. Melles, Menno D. de Jong, Constance Schultsz, Petra Wolffs, Alan McNally, Willem van Schaik, John Penders
Summary: Previous studies have shown a high risk of ESBL-producing Enterobacteriaceae acquisition among international travelers visiting AMR hotspots. This study investigated whether successful colonization of ESBL-E during travel is associated with the composition, functional capacity, and resilience of the traveler's microbiome. The results suggest that changes in the microbial composition and abundance of certain bacteria may play a role in preventing ESBL-E acquisition.
Article
Microbiology
Jacky Flipse, Anne-Marie Niekamp, Anne Dirks, Nicole H. T. M. Dukers-Muijrers, Christian J. P. A. Hoebe, Petra Wolffs, Inge H. M. van Loo
Summary: Treponema pallidum subsp. pallidum is the causative agent of syphilis, and its diagnosis and disease staging are based on clinical findings and serologic testing. PCR analysis of swab samples from genital ulcers is recommended, but its added value is debatable. In this study, both PCR and IgM serology were found to aid in the diagnosis of early syphilis.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Medicine, General & Internal
Tom Schoenmakers, Bas C. T. Van Bussel, Stefan H. M. Gorissen, Inge H. M. Van Loo, Frank Van Rosmalen, Wilhelmine P. H. G. Verboeket-van de Venne, Petra F. G. Wolffs, Walter N. K. A. Van Mook, Mathie P. G. Leers, CoLalC Consortium
Summary: This study aims to investigate whether biochemical and haematological changes due to the patient's host response (CoLab algorithm) in combination with a SARS-CoV-2 viability PCR (v-PCR) can be used to determine when a patient with COVID-19 is no longer infectious. The study consists of three parts using different cohorts of patients, aiming to develop an algorithm, investigate its efficacy, and validate it. The results will be disseminated through peer-reviewed journals and presentations.
Article
Cultural Studies
Alena Kamenshchikova, Petra F. G. Wolffs, Christian J. P. A. Hoebe, John Penders, Klasien Horstman
Summary: Complex phenomena, such as antimicrobial resistance (AMR), are often explained in biomedical sciences using analogies and metaphors. These metaphors play a crucial role in knowledge production and can shape scientific models of thought. Some metaphors, like 'AMR is an apocalypse' or 'antibiotics are weapons', are recognized immediately, but others, such as 'bacterial reservoirs' and 'bacterial colonies', are presented as literal and descriptive. However, these terms still carry historical links and can perpetuate thinking about bacteria as foreign actors.
SCIENCE AS CULTURE
(2023)
Article
Public, Environmental & Occupational Health
Alena Kamenshchikova, Marina M. Fedotova, Olga S. Fedorova, Sergey V. Fedosenko, Petra F. G. Wolffs, Christian J. P. A. Hoebe, Klasien Horstman
Summary: This study analyzes how political programmes for fixing antibiotic behaviours were adapted into the practices of health-care professionals and patients in Russia. It found that the requirement for medical prescriptions does not fulfill the infrastructural gaps that influence antibiotic practices, leading doctors, pharmacists and patients to compensate for the gaps through informal networks of antibiotic care.
SOCIOLOGY OF HEALTH & ILLNESS
(2021)
Article
Microbiology
J. M. van Niekerk, B. M. J. W. van der Veer, C. J. P. A. Hoebe, J. van de Bovenkamp, C. van Herk, I. H. M. van Loo, L. B. van Alphen, P. F. G. Wolffs
Summary: The cobas 4800 CT/NG assay was evaluated for oropharyngeal swabs, demonstrating utility and accuracy for these samples. However, the relatively low oropharyngeal loads, especially for N. gonorrhoeae, may lead to potential missed diagnoses in some cases.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
