Article
Immunology
Yuanyuan Jia, Xiu Zou, Wei Yue, Jin Liu, Ming Yue, Yang Liu, Li Liu, Peng Huang, Yue Feng, Xueshan Xia
Summary: The prevalence of HCV subtypes 3a and 3b is rapidly increasing in Yunnan, China, with a high level of infection attributed to increased drug use. This highlights the importance of implementing more stringent preventative and therapeutic measures to curb the spread of the virus.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Mohammed A. Rohaim, Rania F. El Naggar, Mohammed A. AbdelSabour, Basem A. Ahmed, Mohamed M. Hamoud, Kawkab A. Ahmed, Osama K. Zahran, Muhammad Munir
Summary: This study identified the causative agent of duck viral hepatitis through viral and molecular detection, isolating the causative virus in embryonated duck eggs with complete genome sequencing. The isolates clustered with Chinese duck hepatitis virus 1, potentially aiding in new vaccine manufacturing and more sensitive diagnostic assays. The study also highlighted the importance of DHAV surveillance and re-evaluation of currently used vaccines against newly detected isolates.
Article
Veterinary Sciences
Xinxiu Qian, Liping Hu, Kaichuang Shi, Haina Wei, Yuwen Shi, Xin Hu, Qingan Zhou, Shuping Feng, Feng Long, Shenglan Mo, Zongqiang Li
Summary: This study developed a triplex real-time quantitative PCR (qPCR) method for detection and differentiation of genotypes I and II ASFV. The method demonstrated high sensitivity, specificity, and good repeatability. Testing of clinical samples from Guangxi province revealed a high prevalence of ASFV in the region, with a higher detection rate for genotype II compared to genotype I.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Microbiology
Setsuko Ishida, Shima Yoshizumi, Hidekatsu Sakata, Keiji Matsubayashi
Summary: Hepatitis E virus (HEV) infection is a global public health concern. In this study, a sensitive real-time RT-PCR assay was developed and validated for the detection of all four HEV genotypes. The assay also allowed discrimination of geno-types 1, 2, and 4 from genotype 3 by single nucleotide polymorphism (SNP) analysis.
MICROBIOLOGY SPECTRUM
(2022)
Article
Medicine, General & Internal
Xue-Di Cheng, Hua-Feng Xu, Feng Wei, Li-Xin Jiang, Hai-Zhou Zhou
Summary: This study revealed a higher recombination rate (23%) among HCV patients in Heilongjiang Province, China, with the unique finding of the 2a/1b recombinant in the 5'UTR, E1, and NS5B regions.
Article
Virology
Mohamed El Kassas, Mohamed Alboraie, Heba Omar, Yasmeen Abd El Latif, Mohammed Abd Algaber, Adel El Tahan, Hesham El Halwagy, Shimaa Afify, Magdy Elserafy, Kadry Elsaeed, Wahid Doss
Summary: The study demonstrates the efficacy and safety of the fixed dose combination of ombitasvir-paritaprevir-ritonavir plus ribavirin (OBV/rPTV/RBV) in treating patients with chronic HCV genotype 4. The majority of patients achieved virologic response at posttreatment week 12, and there was significant improvement in liver biochemical profile and fibrosis level at SVR12.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Veterinary Sciences
Xin Hu, Shuping Feng, Kaichuang Shi, Yuwen Shi, Yanwen Yin, Feng Long, Xiankai Wei, Zongqiang Li
Summary: Porcine hemagglutinating encephalomyelitis virus (PHEV), porcine pseudorabies virus (PRV), classical swine fever virus (CSFV), and Japanese encephalitis virus (JEV) can cause similar neurological symptoms in infected pigs, and a quadruplex real-time quantitative RT-PCR (qRT-PCR) assay has been developed to detect and differentiate these viruses with high sensitivity and specificity.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Veterinary Sciences
Hyun Jin Hwang, Yun Seong Choi, Kyungyoung Song, Maciej Frant, Jeong Hee Kim
Summary: A fast and quantitative molecular detection method of African swine fever virus (ASFV) is presented in this study. The method can detect all genotypes of ASFV based on the major capsid protein p72, with high sensitivity and specificity.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Food Science & Technology
Catherine Hennechart-Collette, Oceane Dehan, Michel Laurentie, Audrey Fraisse, Sandra Martin-Latil, Sylvie Perelle
Summary: Viruses, including human norovirus and hepatitis viruses, are a major cause of foodborne disease worldwide. This study validated a new membrane material and method for detecting enteric viruses in tap and bottled water. The results showed that the virus recovery rates varied depending on the water samples tested.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Byoung-Hoon Kang, Youngseop Lee, Eun-Sil Yu, Hamin Na, Minhee Kang, Hee Jae Huh, Ki-Hun Jeong
Summary: The rapid and accurate molecular diagnostics are crucial in the face of pandemic diseases, and the nanoplasmonic PCR provides a new solution for rapid and quantitative molecular diagnostics at point-of-care level.
Article
Chemistry, Multidisciplinary
Jing Tu, Yi Qiao, Yuhan Luo, Naiyun Long, Zuhong Lu
Summary: DNA quantification is crucial in life sciences research, and real-time MDA technology can provide template amount information during whole-genome amplification with a very low detection limit, offering a promising tool to quantify entire DNA without sacrificing sample completeness or introducing redundant steps.
Article
Biotechnology & Applied Microbiology
Beibei Zhang, Jie Fan, Yuhang Luo, Huanhuan Lv, Qin Zhao, Mengnan Fan, Hong Duan, Baoyuan Liu, Yuchen Nan, Yani Sun, En-Min Zhou
Summary: The study developed a competitive ELISA for detecting anti-genotype 1 HEV antibodies in human sera, which could be useful for identifying the source of infecting virus in epidemiological investigations.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Analytical
Liang Gong, Siyue Yao, Yidong He, Chengliang Liu
Summary: A new qPCR data analysis method called AERKM is proposed to achieve more accurate results by introducing amplification efficiency (AE) for error correction. It has been verified to have higher precision and stability in 5-point 10-fold gradient qPCR tests of 63 genes.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Sofia Persson, Erik Alm, Mans Karlsson, Theresa Enkirch, Helene Norder, Ronnie Eriksson, Magnus Simonsson, Patrik Ellstrom
Summary: The study showed that RT-ddPCR had slightly higher precision within runs and significantly higher precision between runs compared to RT-qPCR. Analysis of serum from infected individuals and contaminated food samples revealed good agreement and comparable sensitivities between the two methods.
JOURNAL OF VIROLOGICAL METHODS
(2021)
Article
Microbiology
Fuchun Yang, Peng Liu, Xiaohan Li, Rui Liu, Li Gao, Hongyu Cui, Yanping Zhang, Changjun Liu, Xiaole Qi, Qing Pan, Aijing Liu, Xiaomei Wang, Yulong Gao, Kai Li
Summary: In this study, two DEV recombinants expressing DHAV-3 genes were generated using a fosmid-based rescue system. These recombinant viruses expressed DHAV-3 genes well in infected chicken embryo fibroblasts, induced neutralizing antibodies in ducks, and showed solid protection against lethal DEV challenge and DHAV-3 infection as early as 7 days post-vaccination, demonstrating the potential for bivalent vaccine applications.
FRONTIERS IN MICROBIOLOGY
(2021)