Journal
JOURNAL OF CLINICAL MICROBIOLOGY
Volume 49, Issue 12, Pages 4293-4296Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.01261-11
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Funding
- National Institute of Allergy and Infectious Diseases [AI075565]
- Wendy and Ken Ruby Fund for Excellence in Pediatric Urology Research
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We report the concentration and purification of bacterial 16S rRNA by the use of a biotinylated DNA target-specific capture (TSC) probe. For both cultivated bacterial and urine specimens from urinary tract infection patients, TSC resulted in a 5- to 8-fold improvement in the sensitivity of bacterial detection in a 16S rRNA electrochemical sensor assay.
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