4.7 Article

High-Resolution Melting Analysis for Identification of the Cryptococcus neoformans-Cryptococcus gattii Complex

Journal

JOURNAL OF CLINICAL MICROBIOLOGY
Volume 49, Issue 10, Pages 3663-3666

Publisher

AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.01091-11

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Funding

  1. Spanish Fondo de Investigaciones Sanitarias of the Instituto de Salud Carlos III [PI09/1791]
  2. Spanish Ministry of Science and Innovation [SAF2008-03761]
  3. Fondo de Investigaciones Biomedicas of the Spanish Ministry of Science and Innovation [FI10/00464]
  4. Astellas Pharma
  5. bioMerieux
  6. Gilead Sciences
  7. Merck Sharp and Dohme
  8. Pfizer
  9. Schering Plough
  10. Soria Melguizo SA
  11. European Union
  12. ALBAN program
  13. Spanish Agency for International Cooperation
  14. Spanish Ministry of Culture and Education
  15. Spanish Health Research Fund
  16. Instituto de Salud Carlos III
  17. Ramon Areces Foundation
  18. Mutua Madrilena Foundation

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We have developed a two-step method based on high-resolution melting (HRM) that reliably identifies species from the Cryptococcus species complex (Cryptococcus neoformans var. grubii, Cryptococcus neoformans var. neoformans, and Cryptococcus gattii). Our results indicate that HRM can provide a fast protocol to identify and distinguish among the main Cryptococcus species.

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