☆ 4.7 Article

Novel Nested Direct PCR Technique for Malaria Diagnosis Using Filter Paper Samples

JOURNAL OF CLINICAL MICROBIOLOGY (2011)

Journal

JOURNAL OF CLINICAL MICROBIOLOGY

Volume 49, Issue 4, Pages 1628-1630

Publisher

AMER SOC MICROBIOLOGY

DOI: 10.1128/JCM.01792-10

Keywords

-

Categories

Microbiology

Ask authors/readers for more resources

Protocol

Community support

Reagent

Community support

Abstract

The use of direct nested PCR enables the detection of Plasmodium spp. from blood samples collected on filter papers without requiring the time-consuming procedures associated with DNA extraction. Direct PCR provides a rapid, highly sensitive, and cost-effective alternative to diagnosing malaria using filter paper samples and standard nested PCR.

Authors

I am an author on this paper

Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7

Not enough ratings

Secondary Ratings

Novelty

-

Significance

-

Scientific rigor

-

Rate this paper

Recommended

Article Multidisciplinary Sciences

Nested pool testing strategy for the diagnosis of infectious diseases

Ines Armendariz, Pablo A. Ferrari, Daniel Fraiman, Jose M. Martinez, Hugo G. Menzella, Silvina Ponce Dawson

Summary: The use of droplet digital PCR has been shown to be more sensitive than RT-qPCR, allowing for direct quantification of RNA content and optimizing strategies for better identification of infected individuals.

SCIENTIFIC REPORTS (2021)

Add to Collection

Article Chemistry, Analytical

Rapid diagnosis of COVID-19 using disposal paper capacitive sensor

Partha P. Sahu, Geetartha Sarma, Satyajit Das, Biswajyoti Borkakoty

Summary: Accurate and rapid detection and isolation are crucial for controlling the spread of COVID-19. Currently, the gold standard rRT-PCR tool is time-consuming and complex, requiring expensive equipment. This study focuses on developing a disposable paper capacitance sensor for simple and easy detection.

ANALYTICA CHIMICA ACTA (2023)

Add to Collection

Article Infectious Diseases

Comparative Evaluation of LAMP and Nested PCR for the Rapid Diagnosis of Mycobacterium marinum Infection

Yumiao Feng, Miaomiao Wang, Haiqin Jiang, Ying Shi, Wenyue Zhang, Hongsheng Wang

Summary: This study aimed to compare the rapid diagnostic abilities of polymerase chain reaction (PCR), nested PCR, and loop-mediated isothermal amplification (LAMP) for detecting M. marinum in skin samples. The results showed that LAMP and nested PCR were more sensitive and had a higher detection rate than conventional PCR. Considering its simplicity and faster performance, LAMP is more suitable for rapid diagnosis of M. marinum infection, especially in resource-limited settings.

INFECTION AND DRUG RESISTANCE (2023)

Add to Collection

Article Multidisciplinary Sciences

Discrimination of human papillomavirus genotypes using innovative technique nested-high resolution melting

Melika Alirezaei, Sayed Hussain Mosawi, Ali Afgar, Mehdi Zarean, Tahereh Komeili Movahhed, Vajiheh Abbasi, Reza Fotouhi-Ardakani

Summary: The prompt detection and discrimination of human papillomavirus genotypes is crucial for eliminating cervical cancer. A novel technique based on nested-high resolution melting was designed to detect HPV-positive samples and discriminate genotypes. Despite differences in diagnostic capabilities, nested-qPCR-HRM and sequencing were identified as reliable methods. The bioinformatics analysis and melting temperature studies demonstrated the predictability of HPV genotypes. The use of specific molecular techniques can increase accuracy and reliability in discriminating HPV genotypes.

SCIENTIFIC REPORTS (2022)

Add to Collection

Article Chemistry, Analytical

A novel electrooxidation vapor generation technique for the direct analysis of trace Os in ore/water samples

Lei Cheng, Xin-an Yang, Xiao-na Liu, Wang-bing Zhang

Summary: This paper focuses on the use of anodic oxidation to achieve the gaseous transformation of noble metal Os. The conversion of Os to OsO4 can be carried out continuously and stably using RuO2-based dimensionally stable anode (DSA). The catalytic activity of the material has a significant impact on the conversion of osmium, and the formation of free radicals may be crucial for effective oxidation. The electrocatalytic synthesis of OsO4 combined with ICP-MS enables the oxidation and detection of Os(III) and Os(IV) species, with low detection limit, wide linear range, and excellent anti-interference performance.

ANALYTICA CHIMICA ACTA (2022)

Add to Collection

Article Environmental Sciences

A fast and direct determination of bisphenol S in thermal paper samples using paper spray ionization mass spectrometry

Ricardo Alves Bernardo, Jean Carlos Pereira Sousa, Matheus Gallimberti, Fernando Barbosa Junior, Boniek Gontijo Vaz, Andrea Rodrigues Chaves

Summary: Concerns over human health risks due to the use of bisphenol A in thermal papers have led to its replacement by bisphenol S. Analyzing bisphenols typically involves laborious and time-consuming steps, but a new paper spray ionization mass spectrometry (PSI-MS) method offers a faster and efficient alternative. The study found that bisphenol S concentrations in BPA-free thermal papers ranged from 1.36 to 6.77 mu g g(-1), while BPA concentrations in all samples of thermal paper ranged from 6.56 to 16.4 mu g g(-1).

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2021)

Add to Collection

Article Public, Environmental & Occupational Health

Ultrasensitive qPCR-Based Detection of Plasmodium falciparum in Pregnant Women Using Dried Blood or Whole Blood Pellet Samples Processed through Different DNA Extraction Methods

Queen Saidi, Daniel Minja, Judith Njau, Helle Hansson, Reginald Kavishe, Michael Alifrangis

Summary: This study compared the diagnostic sensitivity of different DNA extraction protocols for detecting low-level Plasmodium falciparum infections. The results showed that using a commercial kit greatly improved the likelihood of detecting positive samples.

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE (2022)

Add to Collection

Article Microbiology

Direct PCR on Tissue Samples To Detect Mycobacterium tuberculosis Complex: an Alternative to the Bacteriological Culture

V Lorente-Leal, E. Liandris, M. Pacciarini, A. Botelho, K. Kenny, B. Loyo, R. Fernandez, J. Bezos, L. Dominguez, L. de Juan, B. Romero

Summary: This study evaluated the analytical and diagnostic performance of a real-time PCR technique targeting bovine tuberculosis, showing higher sensitivity and faster detection compared to traditional culture methods. Despite some cross-reactivity issues, this method is considered a valuable alternative to culture.

JOURNAL OF CLINICAL MICROBIOLOGY (2021)

Add to Collection

Article Chemistry, Physical

Efficient oil-water separation by novel biodegradable all cellulose composite filter paper

Chizhou Wang, Shaodi Wu, Ning Zhang, Zhaoli Jiang, Xianglin Hou, Long Huang, Tiansheng Deng

Summary: By using membrane separation technology and surface modification, a completely biodegradable all cellulose composite filter paper has been developed to efficiently treat oily wastewater, providing a new approach for oil-water separation.

GREEN ENERGY & ENVIRONMENT (2023)

Add to Collection

Article Chemistry, Analytical

Filter paper based SERS substrate for the direct detection of analytes in complex matrices

Harmke S. Siebe, Qinglu Chen, Xinyuan Li, Yikai Xu, Wesley R. Browne, Steven E. J. Bell

Summary: The study presents a simple and scalable spray-deposition technique for fabricating SERS-active paper substrates, combining filtration and enhancement in a single material. These paper substrates show high activity in detecting model analytes and effectively filter interfering particles, enabling SERS detection at nanogram levels without sample pre-treatment.

ANALYST (2021)

Add to Collection

Article Virology

Usefulness of Non-Skin Samples in the PCR Diagnosis of Mpox (Monkeypox)

Sonia Algarate, Jessica Bueno, Maria J. Crusells, Mariano Ara, Henar Alonso, Elena Alvarado, Maria Ducons, Sara Arnal, Rafael Benito

Summary: This study evaluated the usefulness of serum and nasopharyngeal samples for the diagnosis of mpox. MPXV DNA was detected in 63 out of 106 samples from 27 patients. Real-time PCR Ct values were lower in anogenital and skin samples compared to serum and nasopharyngeal samples. Over 90% of anogenital, serum, and skin samples were positive for MPXV using real-time PCR. 66.7% of the 27 MPXV PCR-positive patients had a history of or were co-infected with one to three sexually transmitted infection (STI) agents.

VIRUSES-BASEL (2023)

Add to Collection

Article Infectious Diseases

Diagnosis of Chikungunya Virus in Febrile Patients From a Malaria Holoendemic Area

Line Lobaloba Ingoba, Ayodele Adedoja, Simon Marie Peko, Francesco Vairo, Najmul Haider, Richard Kock, Giuseppe Ippolito, Ali Zumla, Etienne Nguimbi, Srinivas Reddy Pallerla, Thirumalaisamy P. Velavan, Francine Ntoumi

Summary: The study evaluated diagnostic methods for CHIK and malaria in febrile Congolese patients, finding a high rate of co-infection. Real-time PCR was identified as a better tool for detecting actual occurrences of CHIK in a malaria holoendemic area.

INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES (2021)

Add to Collection

Article Spectroscopy

Detection and analysis of rotavirus in clinical stool samples using silver nanoparticle functionalized paper as SERS substrate

Sritam Biswas, Yengkhom Damayanti Devi, Dipjyoti Sarma, Diganta Hatiboruah, Nabadweep Chamuah, Nima D. Namsa, Pabitra Nath

Summary: This study presents a low-cost and sensitive SERS substrate for detecting rotavirus in clinical stool samples. The substrate is fabricated by drop-casting silver nanoparticles on printing-grade paper. It is confirmed that the substrate can enhance the characteristic Raman peaks of rotavirus particles in solution. The proposed substrate has the potential to be widely used in research and clinical laboratories.

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY (2023)

Add to Collection

Review Chemistry, Analytical

Lab-on-Paper Devices for Diagnosis of Human Diseases Using Urine Samples-A Review

Wei-Chun Tai, Yu-Chi Chang, Dean Chou, Lung-Ming Fu

Summary: This review highlights the recent advances in microfluidic lab-on-paper diagnostics for human health monitoring and disease diagnosis. It covers the choice of paper, fabrication methods, detection techniques, and sample pretreatment procedures for lab-on-paper devices, with a focus on disease measurement using urine samples. The review also discusses potential challenges in the future development of commercial microfluidic lab-on-paper platforms for human disease detection.

BIOSENSORS-BASEL (2021)

Add to Collection

Article Infectious Diseases

Comparison of real-time PCR and nested PCR for toxoplasmosis diagnosis in toxoplasmic retinochoroiditis patients

Khadijeh Khanaliha, Farah Bokharaei-Salim, Alireza Hedayatfar, Abdoulreza Esteghamati, Sayyed Amirpooya Alemzadeh, Qasem Asgari, Saba Garshasbi, Borna Salemi

Summary: This study compared real-time PCR with nested PCR using B1, SAG-4, and MAG-1 bradyzoite genes to diagnose toxoplasmosis in toxoplasmic retinochoroiditis patients. Real-time PCR showed higher sensitivity in detecting toxoplasmosis, particularly using PBMC samples. Utilizing B1 and other specific target genes in real-time PCR is recommended for accurate toxoplasmosis diagnosis.

BMC INFECTIOUS DISEASES (2021)

Add to Collection

Article Entomology

First Nationwide Monitoring Program for the Detection of Potentially Invasive Mosquito Species in Austria

Karin Bakran-Lebl, Stefanie Pree, Thomas Brenner, Eleni Daroglou, Barbara Eigner, Antonia Griesbacher, Johanna Gunczy, Peter Hufnagl, Stefanie Jaeger, Hans Jerrentrup, Lisa Klocker, Wolfgang Paill, Jana S. Petermann, Bita Shahi Barogh, Thorsten Schwerte, Carina Suchentrunk, Christian Wieser, Licha N. Wortha, Thomas Zechmeister, David Zezula, Klaus Zimmermann, Carina Zittra, Franz Allerberger, Hans-Peter Fuehrer

Summary: In recent years, several alien mosquito species have been introduced into Austria, posing a threat to public health. This study provides a nationwide overview of the situation of these alien mosquitoes in Austria. The results show the presence and distribution patterns of the Asian tiger mosquito and Asian bush mosquito, as well as the factors influencing their abundance. The findings contribute to a better understanding of the risk of mosquito-borne diseases and provide a useful baseline for monitoring future changes.

INSECTS (2022)

Add to Collection

Article Infectious Diseases

Short-course antibiotic therapy for hospitalized patients with early clinical response in community-acquired pneumonia: a multicentre cohort study

Simone Bastrup Israelsen, Markus Fally, Britta Tarp, Lilian Kolte, Pernille Ravn, Thomas Benfield

Summary: This study aimed to investigate the efficacy and safety of short-course antibiotic therapy in routine clinical settings among patients hospitalized with community-acquired pneumonia (CAP) and early clinical response. The study found similar outcomes in terms of mortality, readmission, and new antibiotic prescriptions between short-course and prolonged-course therapies, supporting the use of short-course therapy in routine clinical settings.

CLINICAL MICROBIOLOGY AND INFECTION (2023)

Add to Collection

Article Parasitology

Autochthonous Onchocerca lupi infection of a domestic dog in Austria

Maria Sophia Unterkoefler, Alexandra Huck, Katja Silbermayr, Hans-Peter Fuehrer

Summary: A case of autochthonous O. lupi infection in a dog from Austria, confirming the potential zoonotic risk of this emerging canine ocular pathogen.

PARASITES & VECTORS (2023)

Add to Collection

Review Parasitology

Canine babesiosis in Austria in the 21st century-A review of cases

Anja Joachim, Maria Sophia Unterkoefler, Anja Strobl, Karin Bakran-Lebl, Hans -Peter Fuehrer, Michael Leschnik

Summary: A retrospective study assessed 699 cases of canine babesiosis in eastern Austria, with 542 cases acquired in Austria and the rest from neighboring countries. The majority of cases were found in Vienna, eastern Lower Austria, and Burgenland. The tick vector of Babesia canis, Dermacentor reticulatus, showed a patchy distribution in Austria. The data suggest that tick control should be advised throughout the year.

VETERINARY PARASITOLOGY- REGIONAL STUDIES AND REPORTS (2023)

Add to Collection

Editorial Material Respiratory System

Core outcome sets, developed collaboratively with patients, can improve the relevance and comparability of clinical trials

Alexander G. Mathioudakis, Ekaterina Khaleva, Markus Fally, Paula R. Williamson, Jens-Ulrik Jensen, Tim W. Felton, Chris Brightling, Andrew Bush, Tonya Winders, John Linnell, Valeria Ramiconi, Courtney Coleman, Tobias Welte, Graham Roberts, Jurgen Vestbo

EUROPEAN RESPIRATORY JOURNAL (2023)

Add to Collection

Article Parasitology

Molecular pathogen screening of louse flies (Diptera: Hippoboscidae) from domestic and wild ruminants in Austria

Miguel Pena-Espinoza, Daniel Em, Bita Shahi-Barogh, Dominik Berer, Georg G. Duscher, Lara van der Vloedt, Walter Glawischnig, Steffen Rehbein, Josef Harl, Maria S. Unterkoefler, Hans-Peter Fuehrer

Summary: In this study, molecular genetics were used to detect and characterize vector-borne pathogens in hippoboscid flies infesting domestic and wild animals in Austria. Several pathogens were found, including some with zoonotic potential. The presence of Trypanosoma sp. in H. equina, a louse fly, was also reported for the first time in Austria. Further research and monitoring are needed to understand the role of these ectoparasites as vectors of infectious agents in a One-Health context.

PARASITES & VECTORS (2023)

Add to Collection

Article Parasitology

Haplotypes of Dirofilaria repens from Poland and selected countries of Central, North-Eastern Europe and the Middle East: An evaluation on the relation between the genetic diversity and the geographic distribution of the fast-spreading parasite

Mustafa Alsarraf, Gad Baneth, Anna Bogucka-Kocka, Lavinia Ciuca, Dorota Dwuznik-Szarek, Hans -Peter Fuehrer, Agnieszka Kloch, Przemyslaw Kolodziej, Viktoria Levytska, Ewa Julia Mierzejewska, Andrei D. Mihalca, Angela Monica Ionica, Andrii Mushynskyi, Yaarit Nachum-Biala, Mohammed Alsarraf, Anna Bajer

Summary: Subcutaneous dirofilariosis is a rapidly spreading infection that affects dogs, carnivores, and even humans. This study aimed to assess the genetic diversity of the parasite and its spread across Europe, finding that climate change, movement of infected hosts, and the diversity of wild reservoirs contribute to the spread of the disease.

VETERINARY PARASITOLOGY (2023)

Add to Collection

Review Microbiology

Relevant Brachycera (Excluding Oestroidea) for Horses in Veterinary Medicine: A Systematic Review

Vicky Frisch, Hans-Peter Fuehrer, Jessika-M. V. Cavalleri

Summary: In equine stables, various insects act as pests or potential vectors, transmitting infectious agents to horses. A systematic review was conducted to investigate the role of Brachycera insects, including Tabanidae, Muscidae, Glossinidae, and Hippoboscidae, in transmitting pathogens to horses. Out of the 38 identified articles, only 7 pathogens were shown to be transmitted by Brachycera. Further studies are needed to understand the significance of Brachycera as vectors for equine health.

PATHOGENS (2023)

Add to Collection

Article Microbiology

Vector-Borne Pathogens in Guard Dogs in Ibadan, Nigeria

Isabella Gruenberger, Amelie-Victoria Liebich, Temitayo Olabisi Ajibade, Oluwasola Olaiya Obebe, Nkiruka Fortunate Ogbonna, Licha N. N. Wortha, Maria S. S. Unterkoefler, Hans-Peter Fuehrer, Adekunle Bamidele Ayinmode

Summary: This study investigated the blood samples of 150 owned guard dogs in the southwest of Nigeria and found that 12% of samples tested positive for at least one pathogen. The prevalence of vector-borne pathogens in these dogs was lower than previous studies, possibly due to regular monitoring and treatment at veterinary clinics.

PATHOGENS (2023)

Add to Collection

Article Biodiversity Conservation

Prevalence, Molecular Characterization, and Ecological Associations of Filarioid Helminths in a Wild Population of Blue Tits (Cyanistes caeruleus)

Jorge Garrido-Bautista, Josef Harl, Hans-Peter Fuehrer, Mar Comas, Steve Smith, Dustin J. Penn, Gregorio Moreno-Rueda

Summary: This study examines the prevalence and associations of microfilariae in a wild population of blue tits, as well as characterizes the filarioid lineages using genetic sequencing. The results show a moderate prevalence of microfilariae in the blue tit population, and a negative association between parasite presence and host body mass. Phylogenetic analyses reveal the presence of five filarioid lineages, four of which belong to the Splendidofilaria clade.

DIVERSITY-BASEL (2023)

Add to Collection

Article Health Care Sciences & Services

Clinical trials of pneumonia management assess heterogeneous outcomes and measurement instruments

Alexander G. Mathioudakis, Markus Fally, Jan Hansel, Rebecca C. Robey, Faiuna Haseeb, Thomas Williams, Ahmed Kouta, Tobias Welte, Dan G. Wootton, Mike Clarke, Grant Waterer, Paul Dark, Paula R. Williamson, Jorgen Vestbo, Timothy W. Felton

Summary: This review identifies significant variations in outcomes and measurement instruments reported in clinical trials of pneumonia management. Important outcomes are often omitted. A rigorous core outcome set is urgently needed to address this issue.

JOURNAL OF CLINICAL EPIDEMIOLOGY (2023)

Add to Collection

Article Parasitology

Diversity and geographic distribution of haplotypes of Dirofilaria immitis across European endemic countries

Mustafa Alsarraf, Elena Carreton, Lavinia Ciuca, Anastasia Diakou, Dorota Dwuznik-Szarek, Hans-Peter Fuehrer, Marco Genchi, Angela Monica Ionica, Agnieszka Kloch, Laura Helen Kramer, Andrei D. Mihalca, Martina Miterpakova, Rodrigo Morchon, Elias Papadopoulos, Mateusz Pekacz, Laura Rinaldi, Mohammed Alsarraf, Mariia Topolnytska, Alice Vismarra, Anna Zawistowska-Deniziak, Anna Bajer

Summary: This study analyzed heartworm isolates from Europe and Bangladesh, and found that the genetic diversity of Dirofilaria immitis is lower and expands slower compared to Dirofilaria repens. The NADH gene shows lower genetic diversity in the Dirofilaria species.

PARASITES & VECTORS (2023)

Add to Collection

Article Parasitology

Occurrence of Thelazia callipaeda and its vector Phortica variegata in Austria and South Tyrol, Italy, and a global comparison by phylogenetic network analysis

Maria Sophia Unterkoefler, Patrick Dengg, Miriam Niederbacher, Sarah Lindorfer, Antonia Eberle, Alexandra Huck, Katalina Staufer, Carina Zittra, Licha Natalia Wortha, Adnan Hodzic, Georg Gerhard Duscher, Josef Harl, Gerhard Schluesslmayr, Marcos Antonio Bezerra-Santos, Domenico Otranto, Katja Silbermayr, Hans-Peter Fuehrer

Summary: The zoonotic nematode Thelazia callipaeda and its vector Phortica variegata were found to naturally occur in Austria. Their presence was confirmed through DNA analysis and morphological identification. However, T. callipaeda was not detected in any of the Phortica specimens.

PARASITES & VECTORS (2023)

Add to Collection

Article Parasitology

Sarcoptes scabiei infestation in a captive lowland tapir (Tapirus terrestris): case report, morphological and molecular genetic mite identification

Perrine Keiser, Christoph Hoerweg, Anna Kuebber-Heiss, Stephan Hering-Hagenbeck, Bita Shahi-Barogh, Katharina Reitl, Hanna Vielgrader, Thomas Voracek, Hans-Peter Fuehrer, David Ebmer

Summary: This study reports a case of chronic Sarcoptes scabiei infestation in a captive lowland tapir at Vienna Zoo. The infestation was successfully diagnosed and treated using deep-skin scrapings and medication. However, the tapir ultimately died from bacterial pneumonia. The study highlights the role of cross-species transmission in the spread of this disease, with urban wildlife potentially serving as a bridge between reservoirs and zoo animals.

PARASITOLOGY RESEARCH (2023)

Add to Collection

Article Microbiology

First Detection and Molecular Analysis of Leishmania infantum DNA in Sand Flies of Kosovo

Betim Xhekaj, Ina Hoxha, Katharina Platzgummer, Edwin Kniha, Julia Walochnik, Kurtesh Sherifi, Agim Rexhepi, Behlul Behluli, Vit Dvorak, Hans-Peter Fuehrer, Adelheid G. Obwaller, Wolfgang Poeppl, Jovana Stefanovska, Aleksandar Cvetkovikj

Summary: This study conducted an extensive survey of sand flies in all districts of Kosovo and reported the first detection of Leishmania infantum DNA in sand flies, indicating local transmission of the pathogen.

PATHOGENS (2023)

Add to Collection

No Data Available

© Peeref 2019-2024. All rights reserved.