4.7 Article

A Novel Eukaryote-Made Thermostable DNA Polymerase Which Is Free from Bacterial DNA Contamination

Journal

JOURNAL OF CLINICAL MICROBIOLOGY
Volume 49, Issue 9, Pages 3316-3320

Publisher

AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.00584-11

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Funding

  1. Japan Society for the Promotion of Science
  2. Nakatani Foundation of Electronic Measuring Technology Advancement
  3. Japan Science and Technology (JST) Agency
  4. Toyama University
  5. Takeda Science Foundation
  6. Charitable Trust Laboratory Medicine Research Foundation of Japan
  7. JST (SENTAN and CREST)
  8. Grants-in-Aid for Scientific Research [22659297] Funding Source: KAKEN

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To achieve the production of a thermostable DNA polymerase free from bacterial DNA contamination, we developed eukaryote-made thermostable DNA (Taq) polymerase. The novel eukaryote-made thermostable DNA polymerase resolves the problem of contaminating bacterial DNA in conventional bacterially made thermostable DNA polymerase as a result of its manufacture and incomplete purification. Using eukaryote-made thermostable DNA polymerase, the sensitive and reliable detection of bacteria becomes feasible for large fields, thereby making the development of a wide range of powerful applications possible.

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