Journal
JOURNAL OF CLINICAL MICROBIOLOGY
Volume 49, Issue 9, Pages 3316-3320Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.00584-11
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Funding
- Japan Society for the Promotion of Science
- Nakatani Foundation of Electronic Measuring Technology Advancement
- Japan Science and Technology (JST) Agency
- Toyama University
- Takeda Science Foundation
- Charitable Trust Laboratory Medicine Research Foundation of Japan
- JST (SENTAN and CREST)
- Grants-in-Aid for Scientific Research [22659297] Funding Source: KAKEN
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To achieve the production of a thermostable DNA polymerase free from bacterial DNA contamination, we developed eukaryote-made thermostable DNA (Taq) polymerase. The novel eukaryote-made thermostable DNA polymerase resolves the problem of contaminating bacterial DNA in conventional bacterially made thermostable DNA polymerase as a result of its manufacture and incomplete purification. Using eukaryote-made thermostable DNA polymerase, the sensitive and reliable detection of bacteria becomes feasible for large fields, thereby making the development of a wide range of powerful applications possible.
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