Journal
JOURNAL OF CLINICAL MICROBIOLOGY
Volume 49, Issue 4, Pages 1537-1541Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.00196-10
Keywords
-
Categories
Funding
- China Mega-Project for Infectious Disease [2008ZX10004-008, 2009ZX10004-101]
Ask authors/readers for more resources
Human bocavirus 2 (HBoV2) is a parvovirus that has been recently identified in stool samples from children. Any association between the virus and clinical disease is unclear. A rapid, reliable diagnostic method is necessary to address this issue. In this study, we developed a sensitive and specific HBoV2 quantitative real-time PCR assay that targets the HBoV2 NP-1 gene, based on the TaqMan method. The assay could reproducibly detect 10 copies of a recombinant DNA plasmid containing a partial region of the HBoV2 genome, with a dynamic range of 8 log units (10(1) to 10(8) copies). A clinical evaluation detected HBoV2 in 85 (24.6%) of 345 children with gastroenteritis, with viral loads ranging from 1.67 x 10(2) to 4.27 x 10(9) copies per ml of stool specimen.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available