Article
Infectious Diseases
Kento Furuya, Kenta Ito, Kyohei Sugiyama, Satoru Makio, Toshio Shimada
Summary: Infections caused by Robinsoniella peoriensis, particularly bacteremia, are rare with only six reported cases. This case report describes a patient with R. peoriensis bacteremia while being treated with piperacillin-tazobactam. The patient's condition improved after switching to meropenem and vancomycin.
JOURNAL OF INFECTION AND CHEMOTHERAPY
(2023)
Article
Immunology
Nese Inal, Alper Karagoz, Egemen Turhan, Gulsen Hazirolan
Summary: This study reported a rare case of osteomyelitis in a three-year-old male patient caused by the isolation of R. peoriensis, which was effectively treated with antibiotics. Antimicrobial susceptibility testing is crucial for guiding treatment decisions.
ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA
(2021)
Article
Virology
Pragya D. Yadav, Nivedita Gupta, Varsha Potdar, Sreelekshmy Mohandas, Rima R. Sahay, Prasad Sarkale, Anita M. Shete, Alpana Razdan, Deepak Y. Patil, Dimpal A. Nyayanit, Yash Joshi, Savita Patil, Triparna Majumdar, Hitesh Dighe, Bharti Malhotra, Jayanthi Shastri, Priya Abraham
Summary: Due to the failure of virus isolation in Vero CCL-81 cells, an in vivo and subsequent in vitro approach were used to isolate the Omicron variant. The virus was successfully isolated from hamster specimens and showed high viral load and cytopathic effects in infected cells. Genome analysis revealed mutations in different host species.
Review
Microbiology
Javier Mejia-Gomez, Tiffany Zigras, Yashesh Patel, Wendy Wolfman, Eleni Philippopoulos, Nada Shaltout, Coleman Rotstein, Stephane Laframboise, Samir N. Patel, Julianne V. Kus, Susan M. Poutanen, Seyed M. Hosseini-Moghaddam
Summary: We present a case report of a 47-year-old woman with concurrent jejunal adenocarcinoma and endometrial cancer, who was admitted with sepsis. Uterine fluid and blood cultures were positive for Robinsoniella peoriensis, which is the first reported case in Canada. Clinicians are encouraged to share their experience in treating gynecologic infections caused by Robinsoniella peoriensis to avoid inadequate antimicrobial treatment and potential mortality.
Letter
Immunology
J. Scott Weese, Kurtis E. Sobkowich, Zvonimir Poljak, Theresa M. Bernardo
Summary: We retrospectively reviewed culture and susceptibility results from 86 veterinary diagnostic laboratory results in US dogs and cats, finding 26 E. menigoseptica, 1 E. miricola, and 59 unspeciated Elizabethkingia isolates from 9 US states (2-22 isolates per state). Elizabethkingia infections in animals might pose an increased risk to humans.
EMERGING INFECTIOUS DISEASES
(2023)
Article
Biochemistry & Molecular Biology
Malgorzata S. Malys, Christof Aigner, Stefan M. Schulz, Helga Schachner, Andrew J. Rees, Renate Kain
Summary: Both differential ultracentrifugation and Exo-spin columns can isolate sEV from human serum, with Exo-spin enabling isolation of sEV with vesicle populations similar to those recovered by DUC, but with lower concentrations. DUC samples contained higher concentrations of exosome specific proteins and mainly consisted of sEV particles, while Exo-spin samples contained copious co-purified plasma lipids.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Emiko Rimbara, Masato Suzuki, Hidenori Matsui, Masahiko Nakamura, Misako Morimoto, Chihiro Sasakawa, Hiroki Masuda, Sachiyo Nomura, Takako Osaki, Noriyo Nagata, Keigo Shibayama, Kengo Tokunaga
Summary: The study successfully cultured Helicobacter suis directly from human stomachs in vitro and isolated it from patients with gastric diseases. Eradication of H. suis led to significant improvements in endoscopic and histopathological findings, while oral infection of mice with H. suis elicited inflammatory responses and gastric mucosal metaplasia progression.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemical Research Methods
Thomas I-H Park, Leon C. D. Smyth, Miranda Aalderink, Zoe R. Woolf, Justin Rustenhoven, Kevin Lee, Deidre Jansson, Amy Smith, Sheryl Feng, Jason Correia, Peter Heppner, Patrick Schweder, Edward Mee, Mike Dragunow
Summary: This article presents a simple and highly reproducible method to culture various types of human brain cells from brain specimens. The method allows for quick preparation of cells and can be used to study human brain cells.
Article
Health Care Sciences & Services
Michael J. Loftus, Richard J. Everts, Allen C. Cheng, Perenise Eti, Timote Fakasiieiki, Lupeoletalelei Isaia, Enita Isopo, Adam W. J. Jenney, Viali Lameko, Hinauri Leaupepe, Folototo Leavai, Sue J. Lee, Mele Moungaevalu, Andrew J. Stewardson, Rosemary Tekoaua, Douglas Tou, Geoffrey Wuatai, Anton Y. Peleg
Summary: This study retrospectively analyzed antimicrobial susceptibility results from the national microbiology laboratories of four Pacific Island countries from 2017 to 2021. It found variations in the prevalence of methicillin resistance among Staphylococcus aureus isolates and high susceptibility of ceftriaxone against Escherichia coli and Klebsiella pneumoniae.
LANCET REGIONAL HEALTH-WESTERN PACIFIC
(2023)
Article
Cell Biology
Emmalee J. Northrop-Albrecht, William R. Taylor, Bing Q. Huang, John B. Kisiel, Fabrice Lucien
Summary: This study evaluated five methods for isolating extracellular vesicles from stool, and found that ultrafiltration and size exclusion chromatography had better performance. The findings provide a foundation for further research on stool-derived extracellular vesicles as novel biomarkers for early colorectal cancer detection.
JOURNAL OF EXTRACELLULAR VESICLES
(2022)
Article
Infectious Diseases
Marah E. Condit, Emma Jones, Brad J. Biggerstaff, Cecilia Y. Kato
Summary: This study validates a simplified method for isolating spotted fever group Rickettsia from clinical samples. The method overcomes sample volume limitations and is useful in clinical diagnostics and research.
PLOS NEGLECTED TROPICAL DISEASES
(2022)
Article
Medicine, Research & Experimental
S. G. Kovalenko, Sh. R. Frolova, V. K. Kramkova, A. K. Berezovskii, M. A. Popov, D. V. Shumakov, D. I. Zybin, E. G. Agafonov, V. V. Dontsov, K. I. Agladze
Summary: To increase the yield and survival of living cells, a study optimized the method for isolating cardiomyocytes, finding that the composition of the buffer solution is more important than its oxygenation.
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE
(2023)
Article
Chemistry, Medicinal
Yingying Sun, Xiu Han, Zhijuan Hu, Tongjie Cheng, Qian Tang, Hui Wang, Xiaoqun Deng, Xu Han
Summary: Marine macroalgae, especially red macroalgae, are considered a good source of mycosporine-like amino acids (MAAs). This study investigated the extraction, isolation, and identification of MAAs from four species of red macroalgae. The results showed that the extraction conditions with 25% methanol or 25% ethanol as solvents resulted in effective extraction of MAAs, with Bangia fusco-purpurea having the highest MAA content among the species studied.
Article
Ophthalmology
Luke A. Halliday, John P. M. Wood, Glyn Chidlow, Robert J. Casson, Dinesh Selva, Michelle T. Sun
Summary: In this study, researchers successfully established cell cultures of human lacrimal gland using biopsy-sized tissue specimens. The cells in the cultures showed three-dimensional organization and a heterogeneous population, including epithelial, myoepithelial, mesenchymal, and progenitor cells.
Article
Multidisciplinary Sciences
Yingchao Meng, Yanan Zhang, Marcel Buhler, Shuchen Wang, Mohammad Asghari, Alessandra Sturchler, Bogdan Mateescu, Tobias Weiss, Stavros Stavrakis, Andrew J. deMello
Summary: A simple viscoelastic-based microfluidic platform is presented for label-free isolation of small extracellular vesicles (sEVs) from human blood, with high purity, recovery rates, and increased yield compared to conventional methods. The clinical utility of the approach is demonstrated by isolating sEVs from blood samples of cancer patients and healthy donors, showing elevated sEV concentrations in the blood of cancer patients.
