Article
Critical Care Medicine
Tristan W. Clark, Kate R. Beard, Nathan J. Brendish, Ahalya K. Malachira, Samuel Mills, Cathleen Chan, Stephen Poole, Sean Ewings, Nick Cortes, Esther Nyimbili, Laura Presland
Summary: The study aimed to evaluate the impact of mPOCT on influenza detection and clinical outcomes in hospitalized patients. The results showed that the mPOCT group had a higher rate of influenza detection, more timely and appropriate antiviral treatment, and isolation facility use, which contributed to improved clinical outcomes.
LANCET RESPIRATORY MEDICINE
(2021)
Review
Infectious Diseases
Sophie Jullien, Felicity Fitzgerald, Suzanne Keddie, Oliver Baerenbold, Quique Bassat, John Bradley, Jane Falconer, Colin Fink, Ruth Keogh, Heidi Hopkins, Marie Voice
Summary: This study evaluated the diagnostic accuracy of the Luminex NxTAG respiratory pathogen panels for detection of respiratory syncytial virus (RSV) and influenza viruses. The results showed high sensitivity and specificity of the test for RSV and influenza A/B. However, the performance of other comparator tests varied. Further research is needed to assess its performance in different populations.
BMC INFECTIOUS DISEASES
(2022)
Article
Virology
Samadhan J. Jadhao, Binh Ha, Courtney McCracken, Tebeb Gebretsadik, Christian Rosas-Salazar, James Chappell, Suman Das, Tina Hartert, Larry J. Anderson
Summary: RSV infection plays a major role in respiratory tract diseases in infants and throughout life. The RSV antibody enzyme-linked immunoassay is sensitive for detecting infant infection, and the two G EIAs can indicate the group of an earlier primary infection.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Review
Immunology
Ting Shi, Sophie Vennard, Francis Jasiewicz, Rory Brogden, Harish Nair
Summary: RSV-ARI in adults with comorbidities is a significant burden, with limited data available. This study estimated the incidence, hospitalization rate, and in-hospital case fatality ratio of RSV-ARI in this population, and found an association between the disease and any comorbidity.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Virology
Jessica Santiago Cruz, Luciano Kleber de Souza Luna, Vitoria Rodrigues Guimaraes Alves, Danielle Dias Conte, Nancy Cristina Junqueira Bellei
Summary: RSV plays a significant role in acute respiratory infections among children. Viral load may impact disease severity and clinical presentation. Age and coinfection with other pathogens can also influence RSV infection outcomes.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Medicine, General & Internal
E. E. Walsh, G. Perez Marc, A. M. Zareba, A. R. Falsey, Q. Jiang, M. Patton, F. P. Polack, C. Llapur, P. A. Doreski, K. Ilangovan, M. Ramet, Y. Fukushima, N. Hussen, L. J. Bont, J. Cardona, E. DeHaan, G. Castillo Villa, M. Ingilizova, D. Eiras, T. Mikati, R. N. Shah, K. Schneider, D. Cooper, K. Koury, M. -M Lino, A. S. Anderson, K. U. Jansen, K. A. Swanson, A. Gurtman, W. C. Gruber, B. Schmoele-Thoma
Summary: The RSVpreF vaccine showed efficacy in preventing RSV-associated lower respiratory tract illness and RSV-associated acute respiratory illness in adults (>= 60 years of age), with no evident safety concerns.
NEW ENGLAND JOURNAL OF MEDICINE
(2023)
Article
Immunology
Jonathan M. Mansbach, Ruth J. Geller, Kohei Hasegawa, Pedro A. Piedra, Vasanthi Avadhanula, James E. Gern, Yury A. Bochkov, Janice A. Espinola, Ashley F. Sullivan, Carlos A. Camargo
Summary: This study found that infants with respiratory syncytial virus (RSV) at hospitalization followed by a new rhinovirus (RV) infection had the highest risk of recurrent wheezing among severe bronchiolitis cases.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Public, Environmental & Occupational Health
A. Chamseddine, N. Soudani, Z. Kanafani, I Alameddine, G. Dbaibo, H. Zaraket, M. El-Fadel
Summary: The study found that influenza virus can be aerosolized beyond 1 meter in patient rooms, with distance and days after hospital admission influencing the positivity rate of the virus in air samples. Only three patients with RSV infection were recruited, and none of them were emitters.
JOURNAL OF HOSPITAL INFECTION
(2021)
Article
Immunology
Natalie Mazur, Yvette N. Lowensteyn, Joukje E. Willemsen, Christopher J. Gill, Leah Forman, Lawrence M. Mwananyanda, Dianna M. Blau, Robert F. Breiman, Shabir A. Madhi, Sana Mahtab, Emily S. Gurley, Shams El Arifeen, Nega Assefa, J. Anthony G. Scott, Dickens Onyango, Beth A. Tippet Barr, Karen L. Kotloff, Samba O. Sow, Inacio Mandomando, Ikechukwu Ogbuanu, Amara Jambai, Quique Bassat, Mauricio T. Caballero, Fernando P. Polack, Saad Omer, Abdul Momin Kazi, Eric A. F. Simoes, Ashish Satav, Louis J. Bont
Summary: The study investigated 829 RSV-related deaths in children under 1 year old from 38 developing countries, including 166 community deaths from 12 countries. It was found that infants who died in the community were younger at the time of death compared to those who died in the hospital. Neonatal deaths were also higher in the community than in hospitals.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Microbiology
Takashi Ono, Koichi Hashimoto, Yohei Kume, Mina Chishiki, Hisao Okabe, Masatoki Sato, Sakurako Norito, Jumpei Aso, Mitsuru Sada, Izumi Mochizuki, Fumi Mashiyama, Naohisa Ishibashi, Shigeo Suzuki, Hiroko Sakuma, Reiko Suwa, Miyuki Kawase, Makoto Takeda, Kazuya Shirato, Hirokazu Kimura, Mitsuaki Hosoya
Summary: Understanding the molecular diversity of human respiratory syncytial viruses during pandemics caused by different viruses can provide insights that can guide public health decisions and vaccine development.
MICROBIOLOGY SPECTRUM
(2023)
Review
Pharmacology & Pharmacy
Farah Elawar, Ahmed K. Oraby, Quinten Kieser, Lionel D. Jensen, Tyce Culp, Frederick G. West, David J. Marchant
Summary: RSV infection is a major cause of pediatric hospitalizations and deaths, with limited treatment options. The development of effective antivirals and vaccines is crucial in combating RSV, despite challenges in therapeutics so far. This review provides insight into current research progress and future considerations in RSV treatment.
PHARMACOLOGY & THERAPEUTICS
(2021)
Article
Microbiology
Shengwei Jin, Hui An, Tong Zhou, Ting Li, Mengzhen Xie, Saijing Chen, Chengshui Chen, Binyu Ying, Zhangye Xu, Xiaokun Li, Ming Li
Summary: The study revealed diverse clinical and immunological responses to COVID-19 infection based on sex and age categories. Female patients showed milder disease severity, negative associations with inflammatory responses, and liver damage. On the other hand, older patients exhibited compromised immune responses, negative associations with CD8(+) T cell count, but positive associations with inflammatory responses and liver damage.
Article
Virology
Guohong Zhu, Dan Xu, Yuanyuan Zhang, Tianlin Wang, Lingyan Zhang, Weizhong Gu, Meiping Shen
Summary: The study found that the rates of viral infection in children vary by different ages and seasons, with the highest positivity rate of adenovirus appearing in the preschool period, respiratory syncytial virus being highest in infants, and influenza virus increasing with age. The total positive rate of viral infection in different seasons is highest in winter, with influenza positivity rate also being highest in winter.
Review
Biochemistry & Molecular Biology
Zifang Shang, Shuguang Tan, Dongli Ma
Summary: Respiratory syncytial virus (RSV) is a significant viral pathogen causing respiratory tract infections in infants, the elderly, and immunocompromised individuals globally. Over the past decade, there has been substantial progress in understanding the structure, function, and interactions of RSV components, as well as the host immune responses to infection. Researchers and pharmaceutical companies have shown widespread interest in searching for effective interventions for RSV infection, leading to the screening of promising monoclonal antibodies, inhibitors, and vaccine candidates.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Virology
Olayinka O. Idris, Olatunji M. Kolawole
Summary: This study evaluated the seroprevalence and molecular characterization of human respiratory syncytial virus and human adenovirus among children in Nigeria. The findings showed a correlation between HRSV and HAdV infection in children with factors such as age, fever, and cough. Furthermore, viral coinfection with HRSV and HAdV was not observed.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Infectious Diseases
Leonard Schuele, Erley Lizarazo-Forero, Katrin Strutzberg-Minder, Sabine Schuetze, Sandra Loebert, Claudia Lambrecht, Jurgen Harlizius, Alex W. Friedrich, Silke Peter, John W. A. Rossen, Natacha Couto
Summary: The emergence of porcine viruses poses a threat to animal and human health, as well as the economic stability of pig farmers worldwide. Next-generation sequencing (NGS) can detect and characterize known and unknown viruses, but new methods like targeted sequence capture (TSC) panels can improve sensitivity. By analyzing samples from both sides of the Dutch-German border, researchers were able to detect more viral species using TSC compared to traditional methods, and identified a close similarity between influenza strains from Germany and the Netherlands through phylogenetic analysis.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Genetics & Heredity
Jordy P. M. Coolen, Casper Jamin, Paul H. M. Savelkoul, John W. A. Rossen, Heiman F. L. Wertheim, Sebastien P. Matamoros, Lieke B. van Alphen
Summary: Whole-genome sequencing is increasingly used for bacterial outbreak surveillance and infection prevention, but there is little understanding of the consistency of reported outbreaks using different bioinformatic workflows. A multi-centre proficiency testing involving 13 major Dutch healthcare-affiliated centres found discrepancies in reported outbreak clusters, even when similar bioinformatic workflows were used. Standardized methods for determining outbreak clusters did not result in uniform composition among the centres.
MICROBIAL GENOMICS
(2021)
Article
Respiratory System
B. Ditz, J. Boekhoudt, N. Couto, C. A. Brandsma, P. S. Hiemstra, G. W. Tew, M. Neighbors, M. A. Grimbaldeston, W. Timens, H. A. M. Kerstjens, J. W. A. Rossen, V Guryev, M. van den Berge, A. Faiz
Summary: Current knowledge about the respiratory microbiome is mainly based on 16S ribosomal RNA gene sequencing. New sequencing approaches, such as metatranscriptomics, offer the ability to measure the viable microbiome response to environmental conditions and investigate host-microbiome interactions. This study used RNA sequencing of bronchial biopsies to analyze the respiratory microbiome in COPD patients, and found ultra-low levels of microbial mass. The study also identified host-microbiome interactions and their regulation in the airways.
COPD-JOURNAL OF CHRONIC OBSTRUCTIVE PULMONARY DISEASE
(2022)
Article
Microbiology
Maaike J. C. van den Beld, John W. A. Rossen, Noah Evers, Mirjam A. M. D. Kooistra-Smid, Frans A. G. Reubsaet
Summary: Three alternative approaches using MALDI-TOF MS were explored to identify and distinguish Shigella spp., E. coli, and EIEC, but none of them were suitable for clinical diagnostics. Other tests should be used for the identification of this complex.
Article
Microbiology
Soe Yu Naing, Joost Hordijk, Birgitta Duim, Els M. Broens, Linda Van der Graaf-van Bloois, John W. Rossen, Joris H. Robben, Masja Leendertse, Jaap A. Wagenaar, Aldert L. Zomer
Summary: Acinetobacter baumannii, a nosocomial pathogen, poses a significant public health threat due to the global spread of multidrug-resistant (MDR) strains. Two MDR A. baumannii outbreaks occurred in a companion animal intensive care unit in the Netherlands. Whole-genome sequencing revealed that the isolates of the two outbreaks were not related. Comparative genome analysis showed that the outbreak isolates contained different gene contents, including mobile genetic elements associated with antimicrobial resistance genes.
Article
Food Science & Technology
Babak Pakbin, Wolfram Manuel Bruck, Samaneh Allahyari, John W. A. Rossen, Razzagh Mahmoudi
Summary: This study investigated the prevalence, genotypic and phenotypic antibiotic resistance profile, and clonal relatedness of C. sakazakii strains isolated from powdered infant formula milk (PIFM) samples collected in Tehran, Iran. The results showed a high prevalence rate of C. sakazakii in PIFM samples, with high resistance to multiple antibiotics and a diverse genetic profile. The study highlights the need for novel microbial surveillance systems to control the contamination of this foodborne pathogen in infant foods.
Article
Virology
Kahren van Eer, Ihsane J. Laabi, Birgit H. B. van Benthem, Renske D. M. Steenbergen, Audrey King
Summary: This study found that concurrent genital-anal HPV infections often have high genital viral load, which can be effectively reduced by vaccination.
TUMOUR VIRUS RESEARCH
(2022)
Article
Microbiology
Nienke A. A. Kok, Nilay Peker, Leonard Schuele, Jessica L. L. de Beer, John W. A. Rossen, Bhanu Sinha, Natacha Couto
Summary: This study developed a method for NGS-based diagnosis of mycobacteria, and demonstrated that pre-treatment with saponin and DNAse I can increase the sensitivity of detection.
FRONTIERS IN MICROBIOLOGY
(2022)
Letter
Infectious Diseases
Hege Vangstein Aamot, Eric C. J. Claas, Natacha Couto, Henrik Westh, Gilbert Greub, John W. A. Rossen
NEW MICROBES AND NEW INFECTIONS
(2022)
Article
Microbiology
K. E. Boiten, E. J. Kuijper, L. Schuele, J. van Prehn, L. G. M. Bode, I. Maat, S. A. V. van Asten, D. W. Notermans, J. W. A. Rossen, A. C. M. Veloo
Summary: Five clinical multidrug-resistant Bacteroides fragilis isolates, with resistance to meropenem and metronidazole, were recovered in the Netherlands between 2014 and 2020. Through sequencing and assembly, various mobile genetic elements (MGEs) and antimicrobial resistance genes (ARGs) were identified. This study discovered novel CTns, Tns, and plasmids in these MDR B. fragilis isolates, expanding our understanding of multidrug resistance and horizontal gene transfer in anaerobic bacteria.
Article
Microbiology
Mathijs J. P. Theelen, Roosmarijn E. C. Luiken, Jaap A. Wagenaar, Marianne Sloet M. van Oldruitenborgh-Oosterbaan, John W. A. Rossen, Femke J. W. C. Schaafstra, David A. van Doorn, Aldert L. Zomer
Summary: This study aimed to investigate the short- and long-term effects of transportation, hospitalisation, and trimethoprim-sulfadiazine (TMS) administration on the faecal microbiota and resistome of healthy horses. The results showed that TMS treatment had a rapid and long-lasting effect on faecal microbiota composition and resistome, making the equine hindgut a reservoir and potential source of resistant bacteria, posing a risk to animal and human health.
Article
Pediatrics
Stephanie C. de Crom, M. Ted van Hinsbergh, Inge A. van Beijsterveldt, A. Marceline van Furth, John W. Rossen, Charlie C. Obihara
Summary: This study examined the motor development of children after HPeV or EV infections and found no significant differences in motor development between infected and uninfected children after 24 months of follow-up.
MINERVA PEDIATRICS
(2023)
Article
Microbiology
Babak Pakbin, John W. A. Rossen, Wolfram Manuel Bruck, Naim Montazeri, Samaneh Allahyari, Shaghayegh Pishkhan Dibazar, Razieh Abdolvahabi, Razzagh Mahmoudi, Amir Peymani, Rasoul Samimi
Summary: The study found a prevalence of different foodborne and zoonotic viruses in raw cow milk samples, with astrovirus and its combination with norovirus GI being the most prevalent virus profiles. The highest correlations were observed among the presence of rotavirus and hepatitis A viruses, and TBEV and norovirus GII.
FEMS MICROBIOLOGY LETTERS
(2022)
Article
Infectious Diseases
Jacky Flipse, John W. A. Rossen, Gertjan H. J. Wagenvoort
Summary: The study compares self-collected gargle fluids and nasopharyngeal swabs as a strategy for diagnosing respiratory viruses and finds that both methods are acceptable and can enhance willingness for participating in screenings or diagnostics for respiratory viruses.
JOURNAL OF CLINICAL VIROLOGY PLUS
(2022)
Article
Virology
Hayley Cassidy, Leonard Schuele, Erley Lizarazo-Forero, Natacha Couto, John W. A. Rossen, Alex W. Friedrich, Coretta van Leer-Buter, Hubert G. M. Niesters
Summary: This study describes a fatal case associated with a chronic untypeable enterovirus infection in an immunocompromised patient. Long-read sequencing was used to identify the enterovirus genotype and track evolutionary changes. The study highlights the importance of next-generation sequencing in detecting and characterizing challenging viral infections.