Article
Chemistry, Analytical
Xiaoping Zhang, Xiang Ren, Yuan Zhong, Konstantin Chingin, Huanwen Chen
Summary: The study detected ketones in exhaled breath using mass spectrometry and developed a fast, sensitive, noninvasive method for ketone analysis. Results showed higher levels of acetone in the breath of smokers compared to non-smokers.
Article
Microbiology
Shariful Islam, Anna C. Callender, Quynh N. Ho, Catherine A. Wakeman
Summary: Pathogens like Staphylococcus aureus can adapt to host-induced selective pressures by developing the fermenting small-colony variant (SCV) phenotype, which is tolerant to certain antibiotics and heme toxicity. This study demonstrates that iron starvation in S. aureus represses respiration and induces the SCV phenotype. It also shows that prolonged growth in iron starvation promotes the emergence of stable aminoglycoside-resistant SCVs.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
Richard A. Proctor
Summary: The concept of commensalism refers to a relationship where different species coexist, benefiting one side. There is ongoing debate about whether Staphylococcus aureus should still be considered a commensal, as it can cause serious infections. It is important to focus on how to approach this microorganism effectively.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Chemistry, Analytical
Yuting Wang, Jingsong Xu, Li Cao, Shuang Yang, Lina Zhao, Lei He, Zhen Shen, Weiwei Lin, Bishen Shi, Qian Liu, Zhi Huang, Min Li, Hua Wang
Summary: Staphylococcus aureus is a versatile and virulent pathogen. A nanoparticle-based culture-free method using vancomycin modified magnetic nanoparticles and droplet digital PCR was developed to rapidly concentrate and detect S. aureus in blood within 3 hours, significantly shortening the detection time compared to traditional methods. The limit of detection was as low as 10 CFU/mL, improving the diagnostic rate of bacteremia. This new method could be a promising tool for early identification and treatment guidance of S. aureus bloodstream infections, thereby reducing mortality.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Chen Ling, Qiaofang Shi, Zhanpeng Wei, Jingjing Zhang, Junjie Hu, Jiying Pei
Summary: A rapid, environmentally friendly wooden-tip electrospray ionization mass spectrometry (ESI-MS) method was introduced for the determination of quinones in PM2.5, urine, and serum, showing high sensitivity and applicability in complex matrices without extensive sample preparation (LOD range: 0.22-1.48 ng).
Article
Pharmacology & Pharmacy
Zidong Qiu, Chaofa Wei, Xiang Li, Changjiangsheng Lai, Zhilai Zhan, Yan Jin, Li Zhou, Qingxiu Hao, Jian Yang, Shuanglong Wang, Liping Kang, Luqi Huang
Summary: The author developed a novel heating online extraction electrospray ionization mass spectrometry (H-oEESI-MS) technique for the rapid and direct analysis of complex traditional Chinese medicines (TCMs) without the need for sample pretreatment or pre-separation steps. A rapid differentiation and authentication strategy for TCMs based on H-oEESI-MS was proposed, and multivariate statistical analysis showed good differentiation and identification of different types of herbal medicines.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2023)
Article
Immunology
Dhanwini Rudraprasad, Mudigunda V. Sushma, Aravind Kumar Rengan, Milind N. Naik, Joveeta Joseph
Summary: In this study, the protein cargo of extracellular vesicles (EVs) in Staphylococcus aureus endophthalmitis was characterized using a mouse model. The researchers identified significant differences in the amounts of certain proteins between the infected and control groups. Annexin A5, cathepsin D, and C5a play important roles in the pathogenesis of endophthalmitis and may serve as prognostic markers.
MICROBES AND INFECTION
(2022)
Article
Food Science & Technology
A. Arun Prince Milton, Kasanchi M. Momin, Sandeep Ghatak, Shiny C. Thomas, G. Bhuvana Priya, M. Angappan, Samir Das, R. K. Sanjukta, K. Puro, I Shakuntala, A. Sen, B. K. Kandpal
Summary: A polymerase spiral reaction (PSR) assay was developed for rapid and sensitive detection of Staphylococcus aureus in pork samples. The assay demonstrated higher sensitivity and specificity compared to traditional PCR methods, offering a cost-effective and user-friendly option for the detection of S. aureus in less-advanced laboratories.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Review
Pharmacology & Pharmacy
Manman Qin, Yuqing Qian, Lu Huang, Chao Zhong, Mingdong Li, Jun Yu, Huanwen Chen
Summary: Extraction electrospray ionization mass spectrometry (EESI-MS) enables effective ionization of trace molecules in complex samples without sample pretreatment, providing high sensitivity, selectivity, and response speed. It also allows for advanced functionalization of high-energy molecular species. This article systematically reviews the effects of operating conditions on the analytical performance of EESI-MS for various pharmaceutical compounds and introduces its potential application in molecular functionalization for drug discovery.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Biochemical Research Methods
Shuanglong Wang, Shan Shan, Jian Yang, Junhui Zhou, Guofeng Wang
Summary: A triangular-shaped flat plastic substrate probe was prepared for direct ESI-MS analysis of untreated chemical and biological samples. Compared to conventional capillary ESI, this plastic probe offers advantages such as lower cost, higher sensitivity, lower sample consumption, longer signal duration, better reproducibility, and faster analysis speed.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Keyuan Pu, Yue Wang, Huiwen Wei, Jun Hu, Jiamin Qiu, Siyu Chen, Qian Liu, Yan Lin, Kwan-Ming Ng
Summary: A sensitive and robust method, probe electrospray ionization mass spectrometry (mu-PESI-MS), was established for high-throughput determination of the geographical origin of red wine.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Review
Chemistry, Analytical
Bin Hu, Zhong-Ping Yao
Summary: This article reviews the technical aspects and important applications of wooden-tip ESI-MS in various fields. Wooden-tip ESI-MS has shown significant achievements in the past 10 years and has the potential for further development and application.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biochemical Research Methods
Rong Chen, Hannah Marie Brown, R. Graham Cooks
Summary: DESI-MS is a suitable method for intraoperative tissue analysis due to its minimal sample preparation and quick molecular diagnostics. Combining multiple metabolites, such as GABA, creatine, carnitine, and hexol, with the established biomarker NAA, allows for improved accuracy in distinguishing brain parenchyma from glioma. The implementation of diagnostic ion abundance ratios based on these metabolites can enhance method robustness and strengthen discriminatory power, leading to an improved DESI-MS method for glioma diagnosis.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Michael Goetze, Lukasz Polewski, Leila Bechtella, Kevin Pagel
Summary: This article introduces a 3D-printable nano-electrospray ionization (nano-ESI) source for Bruker instruments, which can be assembled by anyone with access to a 3D printer. The source can be fitted to any Bruker mass spectrometer with an ionBooster ESI source and only requires minor, reversible changes to the original Bruker hardware. The general utility of the source was demonstrated by recording high-resolution MS spectra of small molecules, intact proteins, as well as complex biological samples in negative and positive ion mode on two different Bruker instruments.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Chemistry, Analytical
Jian Yu, Matthias Hermann, Rachael Smith, Hailey Tomm, Haidy Metwally, Jennifer Kolwich, Chang Liu, J. C. Yves Le Blanc, Thomas R. Covey, Avena C. Ross, Richard Oleschuk
Summary: Mass spectrometry imaging (MSI) has limitations in terms of surface topography and sample preparation. In this study, a conductance-based autosampling platform and multivariant analysis-driven method were used to create a hyperspectral image for the sampling area. Through straightforward color differences, different mass-to-charge ratios (m/z) were annotated. Various natural products were quickly identified, and the dynamic extracellular environment was shown using compound heatmaps. Hyperspectral visualization-based MSI is an efficient strategy for direct and rapid natural product profiling.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Sophie R. Harvey, Carla O'Neale, Kevin L. Schey, Vicki H. Wysocki
Summary: Aquaporin-0 (AQP0) is the most abundant membrane protein in the eye lens, and its post-translational modifications can accumulate with age. Native mass spectrometry and surface-induced dissociation can be used to study these modifications.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Sheng Zhang, Stan Yoo, Dalton T. Snyder, Benjamin B. Katz, Amy Henrickson, Borries Demeler, Vicki H. Wysocki, Adam G. Kreutzer, James S. Nowick
Summary: A beta dimers are neurotoxic and pathologically relevant species in Alzheimer's disease. This paper introduces a stable A beta(C18C33) dimer model that forms pure dimers in lipid environments without aggregation into fibrils. This model is important for amyloid and Alzheimer's disease research.
Review
Chemistry, Multidisciplinary
Dalton T. Snyder, Sophie R. Harvey, Vicki H. Wysocki
Summary: Native mass spectrometry (nMS) is an important tool in structural biology, with great potential due to a range of ionization techniques and new hybrid ion mobility and mass spectrometry systems. This review focuses on the key features and advantages of surface collisions (surface-induced dissociation, SID) for probing connectivity in protein and nucleoprotein complexes, demonstrating the importance of SID and nMS in future structural elucidation of biological assemblies.
Article
Biochemistry & Molecular Biology
Kye Stachowski, Andrew S. Norris, Devante Potter, Vicki H. Wysocki, Mark P. Foster
Summary: In this study, the mechanism of Cre recombinase assembly was revealed, which allows for DNA recognition and specific cleavage through a series of intermediates. The results show the importance of protein and DNA flexibility in site selection and recombination efficiency mediated by Cre.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Chemistry, Analytical
Justin T. Seffernick, S. M. Bargeen Ala m Turzo, Sophie R. . Harvey, Yongseok Kim, Arpad Somogyi, Shir Marciano, Vicki H. Wysocki, Steffen Lindert
Summary: Understanding the relationship between protein structure and experimental data is crucial. In this study, a method was developed to predict the full energy-resolved mass spectrometry (ERMS) plot from the structure. The results showed that the accuracy of predictions depended on the quality of the native crystal structures.
ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
S. M. Bargeen Alam Turzo, Justin T. Seffernick, Amber D. Rolland, Micah T. Donor, Sten Heinze, James S. Prell, Vicki H. Wysocki, Steffen Lindert
Summary: This study develops an algorithm to predict protein collision cross sections (CCS) and integrates experimental ion mobility data into Rosetta-based molecular modeling to predict protein structures from sequence.
NATURE COMMUNICATIONS
(2022)
Editorial Material
Biochemical Research Methods
Vicki Wysocki
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)
Article
Biochemistry & Molecular Biology
Weicheng Li, Andrew S. Norris, Katie Lichtenthal, Skyler Kelly, Elihu C. Ihms, Paul Gollnick, Vicki H. Wysocki, Mark P. Foster
Summary: Homo-oligomeric ligand-activated proteins are widely present in biology and their functions are regulated by allosteric coupling between ligand-binding sites. By combining statistical thermodynamic modeling with native mass spectrometry, the effects of ligand-dependent population shifts and coupling free energy terms can be quantified, providing necessary insights into regulation and enabling a better understanding of cooperativity.
Editorial Material
Biochemical Research Methods
Vicki H. Wysocki
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Biochemistry & Molecular Biology
Sravya Kovvali, Yuan Gao, Austin Cool, Steffen Lindert, Vicki H. Wysocki, Charles E. Bell, Venkat Gopalan
Summary: Amadori rearrangement products are stable sugar-amino acid conjugates that are formed nonenzymatically during food processing. Understanding bacterial utilization of these fructosamines is crucial due to their impact on the animal gut microbiome. In this study, the catalytic mechanism of the deglycase enzyme FrlB was elucidated through crystallography, computational docking, biochemical assays, and native mass spectrometry. The findings provide insights into the structure-function relationship and mechanisms of enzymes.
Article
Chemistry, Multidisciplinary
Thomas Walker, He Mirabel Sun, Tiffany Gunnels, Vicki Wysocki, Arthur Laganowsky, Hays Rye, David Russell
Summary: Variable-temperature electrospray ionization (vT-ESI) native mass spectrometry (nMS) is used to investigate the thermodynamics of ATP binding to the GroEL tetradecamer chaperonin complex. The study reveals strong enthalpy-entropy compensation (EEC) and variations in stepwise free energy changes, which are consistent with the established nested cooperativity model of GroEL-ATP interactions. The entropy-driven ATP binding is attributed to ligand-induced conformational changes and reorganization of water molecules within the GroEL complex.
ACS CENTRAL SCIENCE
(2023)
Article
Biochemical Research Methods
Mengxuan Jia, Yang Song, Chen Du, Vicki H. Wysocki
Summary: The charge partitioning during the dissociation of protein complexes in the gas phase is influenced by various factors, including interfacial interactions, protein flexibility, protein conformation, and dissociation methods. This study used two cysteine-containing homodimer proteins to investigate the charge partitioning behaviors of collision-induced dissociation (CID) and surface-induced dissociation (SID) processes. Results showed that restructuring dominates with CID, while dissociation with symmetric charge partitioning dominates with SID, regardless of the oxidation state of the intramolecular disulfide bonds. Gas collisions reveal the charge-dependent restructuring/unfolding properties of the protein precursor, while surface collisions predominantly lead to more charge-symmetric monomer separation.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Editorial Material
Biochemical Research Methods
Jenny Brodbelt, Vicki Wysocki
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Editorial Material
Biochemical Research Methods
John C. C. Tran, Iain D. G. Campuzano, Erin S. S. Baker, Vicki H. H. Wysocki
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Chemistry, Multidisciplinary
Shir Marciano, Debabrata Dey, Dina Listov, Sarel J. Fleishman, Adar Sonn-Segev, Haydyn Mertens, Florian Busch, Yongseok Kim, Sophie R. Harvey, Vicki H. Wysocki, Gideon Schreiber
Summary: In this study, the oligomeric state of 17 different bacterial proteins in E. coli was re-evaluated using various methods, and it was found that most proteins exhibit multiple oligomeric states. Some proteins did not show mass-action driven equilibrium between oligomeric states. The predicted oligomeric forms underestimated the complexity of protein quaternary structures in solution, while AlphaFold multimer provided an accurate description.
