Article
Gastroenterology & Hepatology
Maximilian Baumgartner, Rebecca Zirnbauer, Sabine Schlager, Daniel Mertens, Nikolaus Gasche, Barbara Sladek, Craig Herbold, Olga Bochkareva, Vera Emelianenko, Harald Vogelsang, Michaela Lang, Anton Klotz, Birgit Moik, Athanasios Makristathis, David Berry, Stefanie Dabsch, Vineeta Khare, Christoph Gasche
Summary: With increasing urbanization and industrialization, the prevalence of inflammatory bowel diseases (IBDs) has been rising over the past two decades. This study found that the emerging pathogen atypical enteropathogenic E. coli (aEPEC) is associated with disease activity in ulcerative colitis (UC) patients, and it may disrupt immune-microbiota homeostasis.
Article
Food Science & Technology
Peter Kotsoana Montso, Cornelius Carlos Bezuidenhout, Charlotte Mienie, Yinka M. Somorin, Olumide A. Odeyemi, Victor Mlambo, Collins Njie Ateba
Summary: This study investigated the genetic diversity, virulence, and antimicrobial resistance of atypical enteropathogenic E. coli O177 strains isolated from cattle feces in South Africa. The findings showed that cattle carry genetically diverse E. coli O177 strains with a repertoire of virulence and resistance genes, highlighting the need for surveillance of multidrug resistant E. coli O177 strains in cattle.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Veterinary Sciences
Andras Adorjan, Akos Thuma, Laszlo Konyves, Istvan Toth
Summary: The study identified seven atypical enteropathogenic E. coli (aEPEC) strains from ducks, geese, turkeys, chickens, and pigeons in Hungary, with four of the isolates being multidrug resistant. The findings suggest that aEPEC may disappear as poultry grow older, but stress and concurrent diseases could contribute to its reappearance in the intestine.
BMC VETERINARY RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
K. Snehaa, Taru Singh, Sajad Ahmad Dar, Shafiul Haque, Vishnampettai G. Ramachandran, Rumpa Saha, Dheeraj Shah, Shukla Das
Summary: This study investigated the prevalence and regional variation of EPEC in paediatric population suffering from diarrhea in East Delhi, India, showing a changing trend of aEPEC over tEPEC in children less than 5 years with diarrhea, emerging as a drug resistant enteropathogen.
BIOMEDICAL JOURNAL
(2021)
Article
Gastroenterology & Hepatology
Jennifer Lising Roxas, Shylaja Ramamurthy, Katie Cocchi, Ilga Rutins, Anusha Harishankar, Al Agellon, John Scott Wilbur, Gresa Sylejmani, Gayatri Vedantam, V. K. Viswanathan
Summary: Bacterial pathogens manipulate host cell structures and functions, contributing to disease symptoms. Enteropathogenic Escherichia coli (EPEC) secretes proteins that alter host cell behavior. Two secreted proteins, EspZ and EspH, have contrasting effects on host mitochondria. EspZ interacts with FIS1 and protects the host mitochondrial network, enhancing host cell viability. In contrast, EspH increases FIS1 and leads to significant mitochondrial fragmentation and host cell death, facilitating pathogen dispersal.
Article
Biochemistry & Molecular Biology
Jorge Soria-Bustos, Zeus Saldana-Ahuactzi, Partha Samadder, Jorge A. Yanez-Santos, Ygnacio Martinez Laguna, Maria L. Cedillo-Ramirez, Jorge A. Giron
Summary: This study investigates the relationship between flagella expression and the type III secretion system (T3SS) in enteropathogenic Escherichia coli (EPEC). The results suggest that the assembly of an intact T3SS is required for flagella production, and certain regulator genes play a role in activating or inhibiting flagella expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Jing Wang, HongBo Jiao, XinFeng Zhang, YuanQing Zhang, Na Sun, Ying Yang, Yi Wei, Bin Hu, Xi Guo
Summary: In this study, the in-depth characterization and functional analysis of the O-antigen gene clusters in EPEC001 and EPEC080 revealed the key role of O-antigen in the pathogenesis of EPEC. Furthermore, the analysis suggested that these two EPEC strains might have acquired the O-antigen gene clusters through recombination or from uncommon ancestors.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Microbiology
Ashok R. Nayak, Pradip K. Singh, Jinlei Zhao, Montserrat Samso, Michael S. Donnenberg
Summary: Type 4 pili are important surface appendages on bacteria and archaea that can extend and retract with great force. This study presents the detailed structure of an enzyme that converts chemical energy to mechanical energy in a human pathogen, revealing a mechanism of action not previously seen in enzymes that power type 4 pilus extension.
Article
Biochemistry & Molecular Biology
Nabi Jomehzadeh, Khadijeh Ahmadi, Hazhir Javaherizadeh, Maryam Afzali
Summary: This study investigated the relationship between integron genes and class A extended-spectrum ESBLs genes in MDR E. coli strains isolated from children with diarrhea in southwestern Iran. The results showed that EPEC is one of the major causes of childhood diarrhea in the region, with a high prevalence of class 1 integrons and ESBLs in EPEC strains. Continuous monitoring of the emergence and expansion of MDR in EPEC strains is necessary.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Amina Ezzeroug Ezzraimi, Nadji Hannachi, Antoine Mariotti, Clara Rolland, Anthony Levasseur, Sophie Alexandra Baron, Jean-Marc Rolain, Laurence Camoin-Jau
Summary: This study aimed to investigate the interaction between Escherichia coli and platelets, evaluate the antibacterial effect of platelets, and assess bacterial effects on platelet activation. The results showed that platelets inhibited the growth of certain strains while promoting the growth of others. The degree of platelet activation was negatively correlated with bacterial growth.
Article
Biotechnology & Applied Microbiology
Vinay Modgil, Harpreet Kaur, Balvinder Mohan, Neelam Taneja
Summary: This study investigated the relationship between virulence genes and biofilm formation in EAEC isolates from children with acute diarrhea and healthy children. The results showed that biofilm formation is associated with the combination of multiple genes.
INDIAN JOURNAL OF MICROBIOLOGY
(2023)
Article
Microbiology
Danielle D. Munhoz, Fernanda F. Santos, Thais Mitsunari, Paulo A. Schuroff, Waldir P. Elias, Eneas Carvalho, Roxane M. F. Piazza
Summary: The study reported the draft genome of a strain isolated from a child with acute diarrhea, which was originally classified as aEPEC but found to be a hybrid strain of ExPEC and aEPEC with multiple virulence factors. This finding expands our knowledge about E. coli pathogenicity and may serve as a source for future comparative studies on virulence characteristics and evolutionary biology.
Article
Microbiology
Fabiola Avelino-Flores, Jorge Soria-Bustos, Zeus Saldana-Ahuactzi, Ygnacio Martinez-Laguna, Jorge A. Yanez-Santos, Maria L. Cedillo-Ramirez, Jorge A. Giron
Summary: The study investigated the influence of various environmental and nutritional signals on the expression of the flagellin gene in EPEC, finding that specific conditions can significantly enhance fliC transcription. When EPEC encounters certain signals in the intestinal niche, it may activate fliC expression to promote growth and colonization in the host.
Article
Chemistry, Multidisciplinary
Zheng Chen, Hailin Wang
Summary: Researching the impact of DNA methyltransferase on antibiotic toxicity can help address antibiotic resistance issues. Our experiments showed that E. coli strains lacking DNA methyltransferase genes were more sensitive to antibiotics.
Article
Agriculture, Dairy & Animal Science
Jonathan J. Lopez-Islas, Estela T. Mendez-Olvera, Daniel Martinez-Gomez, Andres M. Lopez-Perez, Libertad Orozco, Gerardo Suzan, Carlos Eslava
Summary: Emerging diseases pose a constant challenge in public health, and wild carnivores can be carriers of atypical strains of pathogenic microorganisms, highlighting the importance of natural environments in studying emerging diseases.
