Article
Microbiology
Xiaomei Ma, Tianyong Sun, Jiannan Zhou, Mengfan Zhi, Song Shen, Yushang Wang, Xiufeng Gu, Zixuan Li, Haiting Gao, Pingping Wang, Qiang Feng
Summary: This study analyzed the genomic characteristics and pathogenic effectors of F. nucleatum strains, revealing similarities and differences in functional gene compositions and virulence factors among different subspecies/strains of the same oral pathogen. The findings suggest that different strains of F. nucleatum may vary greatly in terms of their pathogenicity, highlighting the importance of considering subspecies/strains in microbial pathogenicity studies.
MICROBIOLOGY SPECTRUM
(2023)
Article
Medicine, Research & Experimental
Natalia Rustetska, Magdalena Szczepaniak, Krzysztof Goryca, Elwira Bakula-Zalewska, Malgorzata Figat, Artur Kowalik, Stanislaw Gozdz, Magdalena Kowalewska
Summary: This study identified tumour-promoting bacteria in vulvar squamous cell carcinoma (VSCC), which are associated with neutrophil recruitment and cancer progression.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Biotechnology & Applied Microbiology
Dexi Bi, Yin Zhu, Yaohui Gao, Hao Li, Xingchen Zhu, Rong Wei, Ruting Xie, Qing Wei, Huanlong Qin
Summary: This study developed a method for detecting Fusobacterium nucleatum infection in colorectal cancer at the subspecies level and observed wide heterogeneity in the subspecies compositions of F. nucleatum in the gut microbiota among CRC patients, with single-subspecies colonization being common.
MICROBIAL BIOTECHNOLOGY
(2021)
Article
Microbiology
Haibo Chang, Rajeev Mishra, Chaoping Cen, Yunqing Tang, Chenchen Ma, Sanjeev Wasti, Yuanyuan Wang, Qianying Ou, Kaining Chen, Jiachao Zhang
Summary: This study analyzed the gut microbiota of CRC patients in Hainan and identified region-specific bacterial and functional biomarkers, which could potentially serve as non-invasive markers for CRC detection. Further research on gut microbiota in different geographic regions, especially in the diverse population of China, is needed to expand the understanding of markers for CRC.
CURRENT MICROBIOLOGY
(2021)
Article
Chemistry, Medicinal
Essie M. Van Zuylen, Scott A. Ferguson, Alan Hughes, David Rennison, Margaret A. Brimble, Gregory M. Cook
Summary: Fusobacterium nucleatum is an opportunistic human pathogen commonly associated with periodontal disease and colorectal cancer. Current treatments using broad-spectrum antibiotics can harm normal microflora, making the development of narrow-spectrum antimicrobials essential. The antimicrobial Zn ionophore PBT2 shows strong inhibitory activity by disrupting metallostasis in F. nucleatum.
ACS INFECTIOUS DISEASES
(2021)
Article
Dentistry, Oral Surgery & Medicine
Hanie Morsi, Makan Golizeh, Noah Brosseau, Amal Idrissi Janati, Elham Emami, Momar Ndao, Simon D. Tran
Summary: This study developed a proteomics approach to directly detect F. nucleatum subspecies from saliva samples, bypassing the need for bacterial culturing. A total of 157 F. nucleatum subspecies proteins were detected, with subsp. nucleatum absent in the patients but present in half of the controls. This novel technique provides a means to investigate the potential role of F. nucleatum subspecies in CRC development.
ARCHIVES OF ORAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tian Zhao, Jiaqi Chen, Shuai Liu, Jie Yang, Juan Wu, Leiying Miao, Weibin Sun
Summary: Periodontitis is a chronic infectious disease that can lead to various dental problems and even tooth loss. The dental plaque biofilm is a crucial factor in the development of periodontitis. Fusobacterium nucleatum plays an important role in the structure and ecology of dental plaque biofilms. Understanding the molecular mechanism of F. nucleatum during biofilm development is essential for controlling periodontitis. This study used RNA-sequencing and quantitative reverse transcriptase PCR (RT-qPCR) to analyze the gene expression profiles of F. nucleatum in the planktonic and biofilm phases. The results revealed significant differences in gene expression between the two states, with several genes related to metabolism, cell growth, division, oxidative stress, virulence, and oral malodor being differentially expressed. Additionally, the study identified lysine as a potential antibacterial and antibiofilm agent against F. nucleatum.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Microbiology
Matthew Scheible, Cuong T. Nguyen, Truc Thanh Luong, Ju Huck Lee, Yi-Wei Chen, Chungyu Chang, Manuel Wittchen, Martha I. Camacho, Bethany L. Tiner, Chenggang Wu, Andreas Tauch, Asis Das, Hung Ton-That
Summary: F. nucleatum is an anaerobic bacterium that colonizes various human tissues. This study uncovered a set of genes in F. nucleatum that play important roles in oxidative stress response and immune evasion, and are critical for its virulence and invasion capabilities.
Article
Biotechnology & Applied Microbiology
Menglin Wang, Benoit Rousseau, Kunyu Qiu, Guannan Huang, Yu Zhang, Hang Su, Christine Le Bihan-Benjamin, Ines Khati, Oliver Artz, Michael B. Foote, Yung-Yi Cheng, Kuo-Hsiung Lee, Michael Z. Miao, Yue Sun, Philippe-Jean Bousquet, Marc Hilmi, Elise Dumas, Anne-Sophie Hamy, Fabien Reyal, Lin Lin, Paul M. Armistead, Wantong Song, Ava Vargason, Janelle C. Arthur, Yun Liu, Jianfeng Guo, Xuefei Zhou, Juliane Nguyen, Yongqun He, Jenny P. -Y. Ting, Aaron C. Anselmo, Leaf Huang
Summary: Research has shown that the tumor microbiota can influence cancer progression. Targeting anaerobic bacteria with pre-resection antibiotics significantly improves disease-free survival in colorectal cancer patients. By designing an antibiotic encapsulated in liposomes, tumor-associated bacteria can be eliminated without disrupting the gut microbiome, leading to enhanced anti-tumoral immunity and potential interventions in microbiome-immunotherapy.
NATURE BIOTECHNOLOGY
(2023)
Article
Infectious Diseases
Yong Kyun Kim, Yewon Eom, Eunsil Kim, Euijin Chang, Seongman Bae, Jiwon Jung, Min Jae Kim, Yong Pil Chong, Sung-Han Kim, Sang-Ho Choi, Sang-Oh Lee, Yang Soo Kim
Summary: This study investigated the prevalence and molecular characteristics of rifampin-resistant (RIF-R) Staphylococcus aureus (S. aureus) blood isolates in South Korea. The results showed that RIF-R S. aureus blood isolates have unique molecular features and are closely associated with rpoB gene mutations.
Article
Dentistry, Oral Surgery & Medicine
Maria Muchova, Dario L. Balacco, Melissa M. Grant, Iain L. C. Chapple, Sarah A. Kuehne, Josefine Hirschfeld
Summary: This study demonstrates that biofilm formation by F. nucleatum in vitro is subspecies- and substrate-specific. Interestingly, F. nucleatum ssp. polymorphum does not appear to form stable single-subspecies continuous layers of biofilm in vitro.
FRONTIERS IN ORAL HEALTH
(2022)
Article
Microbiology
Kiyonobu Honma, Haruka Sasaki, Nobushiro Hamada, Ashu Sharma
Summary: In this study, a regulatory system comprising an ECF sigma factor and a FecR-like anti-sigma factor was identified in T. forsythia, which plays an important role in the induction of beta-glucanase in response to F. nucleatum. The findings suggest the potential development of small-molecule inhibitors targeting beta-glucanase activity or the TfSigG/TfFecR system as therapeutic drugs against dental plaque formation and periodontitis.
JOURNAL OF BACTERIOLOGY
(2022)
Review
Microbiology
Austin Gregory Morrison, Soumyadev Sarkar, Shahid Umar, Sonny T. M. Lee, Sufi Mary Thomas
Summary: This article reviews the current literature on the oral microbiome, including methods, databases, and knowledge gaps. It also provides a detailed analysis of the role of microorganisms in the oral microbiome, particularly in tissue homeostasis. Furthermore, it discusses key bacteria associated with oral diseases and their involvement in stimulating inflammatory cytokines, the role of gingival crevicular fluid in periodontal disease, microbial interaction networks, the influence of planktonic microbiome and cospecies biofilms, and implications of antibiotic resistance. The paper offers a comprehensive literature analysis and identifies knowledge gaps for future studies.
Article
Biochemistry & Molecular Biology
Manuel Adrian Riveros Escalona, Joice de Faria Poloni, Mathias J. Krause, Marcio Dorn
Summary: Colorectal cancer is commonly associated with changes in the gut microbiota, and recent studies have provided a better understanding of the specific species related to its development. However, the importance of certain species and their interactions across different datasets still needs further exploration.
Article
Infectious Diseases
Seongman Bae, Eun Sil Kim, Yun Woo Lee, Jiwon Jung, Min Jae Kim, Yong Pil Chong, Sung-Han Kim, Sang-Ho Choi, Sang-Oh Lee, Yang Soo Kim
Summary: This study evaluated the clinical characteristics of rifampicin-resistant Staphylococcus aureus (MRSA) infections and found a significant association between rifampicin resistance and infection recurrence.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Microbiology
Yeseul Shin, Jayoung Paek, Hongik Kim, Joong-Ki Kook, Young Hyo Chang
Summary: A novel bacterium strain YH-T4B42(T), belonging to the genus Clostridium, was isolated from the large intestine of a mini-pig. It showed the highest sequence similarity with Clostridium aminophilum, Clostridium symbiosum, and Clostridium butyricum. The predominant fatty acids were C-16:0, C(18:1)cis 9, C-14:0, and C-18:0, with major end products of glucose fermentation being lactate, formate, and acetate.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Microbiology
Jayoung Paek, Lu Bai, Yeseul Shin, Hongik Kim, Joong-Ki Kook, Young-Hyo Chang
Summary: Two bacterial strains isolated from soil samples were identified as a new species of Paenibacillus, with unique morphological characteristics and gene sequences. This discovery contributes to the study of microbial diversity and taxonomy.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Immunology
O-Jin Lee, Young Jin Ko, Seul-Bi Lee, Choon Mee Kim, Sook-Jin Jang, Joong-Ki Kook, Yun Kyong Lim, Jong Hee Shin, Myung Geun Shin, Seung Jung Kee, Seok Hoon Jeong, Seong-Ho Kang, Geon Park
Summary: The ASTA MicroIDSys system and Bruker Biotyper system showed similar performance in identifying viridans group streptococci, with high accuracy rates, making them useful for the identification of VGS strains in clinical microbiology laboratories.
MICROBIOLOGY AND IMMUNOLOGY
(2021)
Article
Infectious Diseases
Young Jin Ko, O. Jin Lee, Seul-Bi Lee, Choon-Mee Kim, Jaehyeon Lee, Joong-Ki Kook, Soon-Nang Park, Jong Hee Shin, Soo Hyun Kim, Eun Jeong Won, Geon Park, Seong-Ho Kang, Sook-Jin Jang
Summary: This study compared the accuracy of the ASTA MicroIDSys system and the MALDI Biotyper system in identifying bacterial and yeast strains, and found that the MicroIDSys system showed high accuracy and utility in identifying bacteria and yeast.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2022)
Article
Microbiology
Yeseul Shin, Jayoung Paek, Hongik Kim, Joong-Ki Kook, Young-Hyo Chang
Summary: A novel obligately anaerobic bacterium strain, Faecalicatena absiana sp. nov., was isolated from pig farm faeces dump. Phylogenetic analysis based on gene sequences and characterization of its phenotypic and chemotaxonomic properties revealed that the strain is distinct from other known Faecalicatena species, and represents a new species.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Dae Gyu Kim, Yongseok Choi, Yuno Lee, Semi Lim, Jiwon Kong, JaeHa Song, Younah Roh, Dipesh S. Harmalkar, Kwanshik Lee, Ja-il Goo, Hye Young Cho, Ameeq Ul Mushtaq, Jihye Lee, Song Hwa Park, Doyeun Kim, Byung Soh Min, Kang Young Lee, Young Ho Jeon, Sunkyung Lee, Kyeong Lee, Sunghoon Kim
Summary: AIMP2-DX2 acts as a cancer-specific regulator of KRAS stability and enhances KRAS-driven tumorigenesis by blocking ubiquitin-mediated degradation of KRAS. A small molecule that inhibits the interaction between AIMP2-DX2 and KRAS reduces KRAS levels and suppresses KRAS-dependent cancer cell growth in vitro and in vivo.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Medicinal
Seunghwan Shim, Da Un Jeong, Hyemi Kim, Chae Yun Kim, Hyejun Park, Yinglan Jin, Kyung Min Kim, Hwa Jeong Lee, Dong Hwan Kim, Yun Soo Bae, Yongseok Choi
Summary: This study designed and synthesized a series of novel Nox inhibitors, which showed regulatory effects on neuronal inflammation in vitro and in vivo experiments, and demonstrated therapeutic efficacy in a PD animal model.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Sreenivasulu Godesi, Joohan Lee, Hossam Nada, Guofeng Quan, Ahmed Elkamhawy, Yongseok Choi, Kyeong Lee
Summary: The proto-oncogenic protein c-KIT is crucial in regulating cellular transformation and differentiation, and dysregulation of c-KIT can lead to various human cancers, especially GISTs. Current approved drugs have limitations, highlighting the need for selective c-KIT inhibitors. Recent research efforts in medicinal chemistry discuss the development of potent c-KIT inhibitors with high selectivity for GISTs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Medicinal
Hossam Nada, Aneesh Sivaraman, Qili Lu, Kyoungho Min, Sungdo Kim, Ja-Il Goo, Yongseok Choi, Kyeong Lee
Summary: Interleukin-6 (IL-6) is a significant factor in inflammatory and autoimmune diseases, and its targeted therapy is promising but still faces challenges. This study focuses on the development of small molecule inhibitors for IL-6 through analysis of protein-protein inhibitors targeting the IL-6/IL-6 receptor/gp130 complex.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Chemistry, Medicinal
Sreenivasulu Godesi, Jeong-Ran Han, Jang-Keun Kim, Dong-Ik Kwak, Joohan Lee, Hossam Nada, Minkyoung Kim, Hyun-A Yang, Joo-Young Im, Hyun Seung Ban, Chang Hoon Lee, Yongseok Choi, Misun Won, Kyeong Lee
Summary: In this study, a series of novel dual MDH1/2 inhibitors were designed and synthesized, and their structure-activity relationship was investigated. Among the tested compounds, compound 50 containing a piperidine ring showed improved growth inhibition of A549 and H460 lung cancer cell lines compared to LW1497. Compound 50 reduced the total ATP content in A549 cells in a dose-dependent manner and suppressed the accumulation of HIF-1a and expression of HIF-1a target genes such as GLUT1 and PDK1. Furthermore, compound 50 inhibited HIF-1a-regulated CD73 expression under hypoxia in A549 lung cancer cells. These results suggest that compound 50 may be a potential candidate for the development of next-generation dual MDH1/2 inhibitors to target lung cancer.
Article
Biochemistry & Molecular Biology
Sreenivasulu Godesi, Hossam Nada, Joohan Lee, Joon-Hee Kang, Soo-Youl Kim, Yongseok Choi, Kyeong Lee
Summary: Each year, millions of new cancer cases are reported, resulting in millions of deaths worldwide. Breast and colon cancers are the most commonly detected variations. To address this increase, the development of innovative therapies is crucial. Small molecules containing pyridine and urea have been found in many anticancer agents, particularly VEGFR2 inhibitors. Hybrid small molecules combining urea and pyridine were designed and tested against breast and colon cancer cell lines, showing potent anticancer activity. Compound 8a displayed a remarkable GI(50) value of 0.06 μM against the MCF7 cancer cell line, while compound 8h exhibited the highest cytotoxic activity against the HCT116 cell line, with a GI(50) of 0.33 ± 0.042 μM. Importantly, compounds 8a, 8h, and 8i demonstrated excellent safety profiles on normal cells. Molecular docking, dynamic studies, and free energy calculations confirmed the affinity of these compounds as VEGFR2 inhibitors.
Article
Biochemistry & Molecular Biology
Kwanshik Lee, Hossam Nada, Minkyoung Kim, Hyejun Park, Kiho Lee, Dongho Seo, Kyeong Lee, Yongseok Choi
Summary: The diamondback moth is a resistant insect pest that poses a threat to brassicaceous crops worldwide. Scientists have synthesized new cantharidin-based compounds that exhibit strong insecticidal activity against the diamondback moth, including a 100% mortality rate at a concentration of 100 mg/L. These compounds also show an anti-feeding effect on cabbage leaves.
Review
Urology & Nephrology
Hye Eun Lee, Seunghwan Shim, Yongseok Choi, Yun Soo Bae
Summary: Oxidative stress can lead to the generation of uncontrolled reactive oxygen species (ROS), causing damage to cells and tissues. While excessive levels of ROS are harmful, moderate levels are essential for normal physiological function. This review discusses the use of Nox inhibitors as a way to regulate ROS levels and provide therapeutic opportunities for diabetic nephropathy.
KIDNEY RESEARCH AND CLINICAL PRACTICE
(2022)
Article
Medicine, General & Internal
Shin Young Yun, Yunhee Lee, Juwon Hong, Dong-Chan Kim, Hyukmin Lee, Dongeun Yong, Yun Kyong Lim, Joong-Ki Kook, Kyungwon Lee
Summary: The aim of this study was to improve the accuracy and efficacy of identifying Fusobacterium species in clinical laboratories. Through the identification of 229 Fusobacterium isolates, the updated ASTA MicroIDSys system was found to be able to effectively identify nine species of Fusobacterium and four subspecies of F. nucleatum.
YONSEI MEDICAL JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Hitesh B. Jalani, Aneesh Sivaraman, Kyoung Ho Min, Hwa Young Lee, Na Kyoung Oh, Hyeseul Kang, Gyochang Keum, Yongseok Choi, Kyeong Lee
Summary: An expeditious one-pot method for the synthesis of highly substituted 5-aminoimidazoles from glyoxals, anilines, and amidines under metal/catalyst-free conditions operative at room temperature is proposed. The method is environmentally friendly and can be applied to the synthesis of bioactive and medicinal compounds.
