Journal
JOURNAL OF CLINICAL MICROBIOLOGY
Volume 47, Issue 3, Pages 674-679Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.01028-08
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Funding
- Federal Ministry of Food, Agriculture and Consumer Protection [FSI 1-1.1.2.]
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The spread of highly pathogenic avian influenza A virus (HPAIV) of subtype H5N1 demands fast and reliable methods for in-depth, full-length sequence analysis. For this purpose, we designed a simple and sensitive method for the preparation of sequencing libraries from H5N1 HPAIV diagnostic RNA samples for sequencing with the Genome Sequencer FLX instrument. The method presented seamlessly integrates high-throughput pyrosequencing with the Roche/454 instrument into diagnostics without the need for additional equipment or molecular biological techniques besides standard PCR and the Genome Sequencer FLX sample preparation and sequencing pipeline.
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