Article
Biochemistry & Molecular Biology
Dessislava Malinova, Laabiah Wasim, Rebecca Newman, Ana Martinez-Riano, Niklas Engels, Pavel Tolar
Summary: The research identified the crucial role of B-cell endosomal trafficking pathways in regulating antigen uptake, B-cell proliferation, and antibody responses, especially in germinal center B-cell reactions and high-affinity antibody production.
Article
Microbiology
Joanne Dai, Elliott D. Sorelle, Emma Heckenberg, Lingyun Song, Jana M. Cable, Gregory E. Crawford, Micah A. Luftig, Alan N. Engelman
Summary: This study demonstrates that EBV infection promotes the proliferation and gene expression profile changes of B cells, and reveals the regulatory mechanism of BFL-1 expression, which contributes to the survival of B cells.
Article
Cell Biology
Zhihua Zheng, Man Zhao, Hao Shan, Dongmei Fang, Zuyi Jin, Jiuge Tang, Zhiping Liu, Liang Hong, Peiqing Liu, Min Li
Summary: In this study, the researchers found that STING1 activation can induce both canonical and non-canonical autophagy. They also discovered that STING1-induced NCA is an efficient antiviral pathway that can effectively inhibit DNA virus HSV-1.
Article
Immunology
Seema R. Patel, Taran S. Lundgren, Wallace Hunter Baldwin, Courtney Cox, Ernest T. Parker, John F. Healey, Ryan P. Jajosky, Patricia E. Zerra, Cassandra D. Josephson, Christopher B. Doering, Sean R. Stowell, Shannon L. Meeks
Summary: The immune response to factor VIII in patients with hemophilia A differs between individuals due to genetic factors, which may explain the variation in inhibitor formation. This study highlights the underappreciated immunological pathway of humoral immunity to factor VIII and lays the foundation for identifying biomarkers to develop tolerance against factor VIII.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Jhon R. Enterina, Susmita Sarkar, Laura Streith, Jaesoo Jung, Britni M. Arlian, Sarah J. Meyer, Hiromu Takematsu, Changchun Xiao, Troy A. Baldwin, Lars Nitschke, Mark J. Shlomchick, James C. Paulson, Matthew S. Macauley
Summary: Germinal centers are critical for antibody affinity maturation, and glycan remodeling plays a key role in maintaining B cells in the germinal center. Downregulation of CD22 ligands on B cells in the germinal center leads to increased apoptosis, reduced production of memory B cells and plasma cells, and delayed affinity maturation of antibodies. These defects are CD22 dependent.
Review
Immunology
Yisi Lu, Joe Craft
Summary: T follicular regulatory cells are a unique subset of regulatory T cells that migrate to B cell follicles and germinal centers to maintain immune homeostasis. They use specific transcription factors to regulate immune responses, promoting humoral immunity and memory following viral infections.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Danica F. Besavilla, Laura Reusch, Josue Enriquez, Karin Schon, Davide Angeletti
Summary: This study reveals the impact of CD4+ T cells on B cell and antibody responses during influenza virus infection, showing that they promote the generation of nucleoprotein-specific B cells and antibodies. However, pre-existing CD4+ T cells do not increase the frequency of antigen-specific germinal center and memory B cells.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Yuexia Liao, Lijuan Fan, Peng Bin, Congrui Zhu, Qingyi Chen, Yepeng Cai, Jielin Duan, Qian Cai, Wei Han, Shizhen Ding, Xiangyu Hu, Yiran Zhang, Yulong Yin, Wenkai Ren
Summary: Recent findings suggest potential connections between neurotransmitters, intestinal mucosal immune responses, and the development of IgA nephropathy (IgAN). This study demonstrates that deficiency of the gamma-amino butyric acid transporter-2 (GAT-2) enhances the differentiation of germinal center (GC) B cells in the intestines and worsens the symptoms of IgAN in a mouse model. Additionally, IgAN patients have lower GAT-2 expression and higher activation of the mammalian target of rapamycin complex 1 (mTORC1) in blood B cells, both of which are correlated with kidney function in IgAN patients.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Immunology
Dominik Schmiedel, Hadas Hezroni, Amit Hamburg, Ziv Shulman
Summary: The research reveals that Brg1 plays a critical role in B cell proliferation and germinal center formation through selective interactions with enhancers. Recruitment of Brg1 to enhancers increases chromatin accessibility, leading to the regulation of gene expression associated with cell cycle and supporting B cell proliferation and the formation of germinal center reactions.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Oncology
Natalie S. Firmino, Rachel A. Cederberg, Che-Min Lee, Rocky Shi, Brennan J. Wadsworth, S. Elizabeth Franks, Kiersten N. Thomas, Lisa R. Decotret, Kevin L. Bennewith
Summary: The study showed that tumor-reactive germinal centers (GCs) exhibit hypoxia, which negatively regulates tumor-induced humoral immune responses. GC hypoxia is associated with levels of antibody-secreting B cells and impairs B cell proliferation and class-switching in vitro.
Article
Immunology
Sayaka Ishihara, Tsuyoshi Sato, Risa Sugioka, Ryota Miwa, Haruka Saito, Ryota Sato, Hidehiro Fukuyama, Akihiko Nakajima, Satoshi Sawai, Ai Kotani, Koko Katagiri
Summary: The regulation of integrins by Rap1 is essential for lymphocyte recirculation, as demonstrated in mice with B-cell-specific Rap1a/b double knockouts. This deficiency leads to a significant decrease in B cells in lymph nodes and impaired germinal center formation and antibody production upon immunization. Additionally, Rap1 deficiency affects the positioning of B cells and their interaction with stromal cells, influencing humoral responses and B-1a cell generation.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Yiping Wang, April Feswick, Vasiliki Apostolou, Petko M. Petkov, Emily K. Moser, Scott A. Tibbetts
Summary: The germinal center (GC) is important for the generation of antigen-specific B cells and antibodies. The protein EWSR1 functions as a negative regulator of B cell responses, and its deficiency leads to an increase in precursor GC B cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Plant Sciences
Ping Wang, Natalie M. Clark, Trevor M. Nolan, Gaoyuan Song, Olivia G. Whitham, Ching-Yi Liao, Christian Montes-Serey, Diane C. Bassham, Justin W. Walley, Yanhai Yin, Hongqing Guo
Summary: This study reveals a previously unknown mechanism by which FER negatively regulates autophagy through TOR.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Jiajia Wang, Jiaying Wang, Wenxiang Hong, Lulu Zhang, Liqian Song, Qi Shi, Yanfei Shao, Guifeng Hao, Chunyan Fang, Yueping Qiu, Lijun Yang, Zhaoxu Yang, Jincheng Wang, Ji Cao, Bo Yang, Qiaojun He, Qinjie Weng
Summary: Optineurin (OPTN) plays a crucial role in regulating dendritic cell (DC) maturation, where its deficiency leads to Jak2/Stat3 activation and IL-10 production, suppressing DC maturation and function to ameliorate autoimmune responses in mice.
NATURE COMMUNICATIONS
(2021)
Article
Pharmacology & Pharmacy
Xin Zhang, Mengjun Zou, Yue Liang, Yonghong Yang, Lina Jing, Minghui Sun, Zhilin Dong, Xinyuan Zhang, Huabao Xiong, Guanjun Dong
Summary: Arctigenin can regulate the differentiation of germinal center B cells in systemic lupus erythematosus (SLE) by inhibiting the IFN-I signaling pathway, leading to the alleviation of disease progression.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Article
Immunology
Samuel W. Du, Tanvi Arkatkar, Holly M. Jacobs, David J. Rawlings, Shaun W. Jackson
EUROPEAN JOURNAL OF IMMUNOLOGY
(2019)
Article
Immunology
Mark T. Orr, Amit P. Khandhar, Emilie Seydoux, Hong Liang, Emily Gage, Traci Mikasa, Elyse L. Beebe, Nicholas D. Rintala, Karin H. Persson, Anwar Ahniyaz, Darrick Carter, Steven G. Reed, Christopher B. Fox
Article
Immunology
Hong Liang, David Poncet, Emilie Seydoux, Nicholas D. Rintala, Milton Maciel, Sophie Ruiz, Mark T. Orr
Article
Immunology
Samuel W. Du, Tanvi Arkatkar, Fahd Al Qureshah, Holly M. Jacobs, Christopher D. Thouvenel, Kristy Chiang, Andrea D. Largent, Quan-Zhen Li, Baidong Hou, David J. Rawlings, Shaun W. Jackson
JOURNAL OF IMMUNOLOGY
(2019)
Article
Immunology
Patricia M. Santos, Juraj Adamik, Timothy R. Howes, Samuel Du, Lazar Vujanovic, Sarah Warren, Andrea Gambotto, John M. Kirkwood, Lisa H. Butterfield
JOURNAL OF EXPERIMENTAL MEDICINE
(2020)
Article
Biochemical Research Methods
Rachael E. Whaley, Sarah Ameny, Tanvi Arkatkar, Aaron Seese, Abigail Wall, Iram Khan, Joseph J. Carter, Erin M. Scherer, David J. Rawlings, Denise A. Galloway, M. Juliana McElrath, Kristen W. Cohen, Andrew T. McGuire
Summary: Isolation of human monoclonal antibodies from natural infection with human pathogens not only helps understand the host response to infection at a molecular level, but also reveals vulnerabilities on pathogens and their biological functions. A cost-effective feeder cell line expressing CD40-ligand, interleukin-2, and interleukin-21 has been developed to isolate single B cells and sustain antibody production, allowing functional screening and subsequent recovery for recombinant antibody cloning. This approach has been successfully used to isolate B cells secreting antibodies neutralizing human papilloma virus from participants in an HPV vaccine study.
JOURNAL OF IMMUNOLOGICAL METHODS
(2021)
Article
Immunology
Holly M. Jacobs, Tanvi Arkatkar, Samuel W. Du, Nicole E. Scharping, Jonathan Woods, Quan-Zhen Li, Kelly L. Hudkins, Charles E. Alpers, David J. Rawlings, Shaun W. Jackson
Summary: Polymorphisms in TACI are associated with various human immune disorders, with subtle variations impacting B-cell biology. The complexity of TACI biology involves both positive and negative regulation of physiological and pathogenic B-cell responses.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Samagya Banskota, Aditya Raguram, Susie Suh, Samuel W. Du, Jessie R. Davis, Elliot H. Choi, Xiao Wang, Sarah C. Nielsen, Gregory A. Newby, Peyton B. Randolph, Mark J. Osborn, Kiran Musunuru, Krzysztof Palczewski, David R. Liu
Summary: We have developed and applied engineered DNA-free virus-like particles (eVLPs) for efficient delivery of gene editing tools, achieving high levels of base editing. By overcoming the limitations of cargo packaging, release, and localization, the fourth-generation eVLPs demonstrated efficient base editing in mouse and human cells. In addition, the off-target effects of eVLPs were significantly reduced compared to other delivery methods such as AAV or plasmid.
Article
Immunology
Kristy Chiang, Andrea D. Largent, Tanvi Arkatkar, Christopher D. Thouvenel, Samuel W. Du, Natali Shumlak, Jonathan Woods, Quan-Zhen Li, Yifan Liu, Baidong Hou, David J. Rawlings, Shaun W. Jackson
Summary: The deletion of B cell-intrinsic CD80/CD86 unexpectedly leads to the disappearance of germinal centers in lupus mice, impacting the production of serum autoantibodies. The absence of germinal centers is associated with the loss of RNA-associated autoantibodies, while preserving anti-dsDNA and connective tissue autoantibody titers.
JOURNAL OF IMMUNOLOGY
(2021)
Review
Neurosciences
Samuel W. Du, Krzysztof Palczewski
Summary: MicroRNAs are short, evolutionarily conserved noncoding RNAs that are critical for controlling normal cellular physiology. Recent research has highlighted their role in supporting retina function through regulating RPE activities. They have potential applications as therapeutics and biomarkers for visual diseases.
TRENDS IN NEUROSCIENCES
(2022)
Article
Multidisciplinary Sciences
Elliot H. Choi, Susie Suh, Andrzej T. Foik, Henri Leinonen, Gregory A. Newby, Xin D. Gao, Samagya Banskota, Thanh Hoang, Samuel W. Du, Zhiqian Dong, Aditya Raguram, Sajeev Kohli, Seth Blackshaw, David C. Lyon, David R. Liu, Krzysztof Palczewski
Summary: The study demonstrates that in vivo base editing can prolong the survival of cone photoreceptors and restore their function in a mouse model of Leber congenital amaurosis.
NATURE COMMUNICATIONS
(2022)
Editorial Material
Immunology
Samuel W. W. Du, Krzysztof Palczewski
Summary: CRISPR/Cas9 genome editing techniques have the potential to treat previously untreatable inherited genetic disorders of vision by correcting mutations that cause these afflictions. Using a prime editor, Qin et al. (2023. J. Exp. Med. https://doi.org/10.1084/jem.20220776) restored visual functions in a mouse model (rd10) of retinitis pigmentosa.
JOURNAL OF EXPERIMENTAL MEDICINE
(2023)
Article
Chemistry, Multidisciplinary
Amit P. Khandhar, Hong Liang, Adrian C. Simpson, Steven G. Reed, Darrick Carter, Christopher B. Fox, Mark T. Orr
Article
Immunology
Jacquelyn A. Gorman, Christian Hundhausen, Mackenzie Kinsman, Tanvi Arkatkar, Eric J. Allenspach, Courtnee Clough, Samuel E. West, Kerri Thomas, Ahmet Eken, Socheath Khim, Malika Hale, Mohamed Oukka, Shaun W. Jackson, Karen Cerosaletti, Jane H. Buckner, David J. Rawlings
FRONTIERS IN IMMUNOLOGY
(2019)