Article
Cell Biology
Gianmarco Pallavicini, Marta Gai, Giorgia Iegiani, Gaia Elena Berto, Annie Adrait, Yohann Coute, Ferdinando Di Cunto
Summary: Goldberg-Shprintzen disease is a rare microcephaly syndrome associated with recessive mutations in the KIF1BP gene, potentially leading to microcephaly due to abnormalities in cell division.
JOURNAL OF CELL SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Zhiqiang Chen, Xinyi Zhou, Xiaojun Zhou, Yi Tang, Mingzhu Lu, Jianhong Zhao, Chenhui Tian, Mingzhi Wu, Yanliang Liu, Edward V. Prochownik, Fubing Wang, Youjun Li
Summary: This study discovers the role of the enzyme PMVK in stabilizing beta-catenin signaling by screening a human genome-wide library. PMVK-produced MVA-5PP competitively binds to CKI alpha to prevent beta-catenin Ser45 phosphorylation and degradation, while PMVK functions as a protein kinase to directly phosphorylate beta-catenin Ser184, increasing its nuclear localization. This non-canonical function of PMVK and its link to the mevalonate pathway reveal a potential target for cancer therapy.
Article
Biology
Juil Kim, Yuseok Moon
Summary: Kim and Moon discovered that the transcription factor PRDM1 is upregulated in colorectal cancer (CRC) cells in response to ribosomal dysfunction, leading to chemoresistance through the IGF signaling pathway. They also found that PRDM1 promotes cancer cell stemness and survival, providing insights into environmental stress-mediated signaling in CRC.
COMMUNICATIONS BIOLOGY
(2021)
Review
Cell Biology
Stefano Sechi, Roberto Piergentili, Maria Grazia Giansanti
Summary: Cytokinesis, the final stage of cell division, plays a crucial role in normal development and preventing cancer. Kinase-mediated phosphorylation is a key process in regulating cytokinesis. While some kinases have well-established roles, others have been less studied but may uncover new pathways of cell division regulation.
Article
Chemistry, Multidisciplinary
Pasquale Sacco, Francesco Piazza, Chiara Pizzolitto, Gabriele Baj, Francesco Brun, Eleonora Marsich, Ivan Donati
Summary: This study presents a strategy to control the dissipation rate of viscoplastic substrates while precisely regulating linear elasticity, and reveals the crucial link between this material property and cellular responses in 2D cultures.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Review
Biology
Ronja Reinhardt, Thomas A. Leonard
Summary: Phosphorylation of proteins is a common mechanism for regulating their function, localization, or activity. Protein kinases, which use ATP to phosphorylate protein substrates, are important signal transducers in cells. The mechanism of phosphoryl-transfer is conserved among protein kinases, and the precise mechanism of autophosphorylation is still a topic of debate. In this review, we evaluate different proposed mechanisms of autoregulation in protein kinases and discuss their implications in physiological signaling networks.
Article
Multidisciplinary Sciences
Anna Bianchi-Smiraglia, David W. Wolff, Daniel J. Marston, Zhiyong Deng, Zhannan Han, Sudha Moparthy, Rebecca M. Wombacher, Ashley L. Mussell, Shichen Shen, Jialin Chen, Dong-Hyun Yun, Anderson O'Brien Cox, Cristina M. Furdui, Edward Hurley, Maria Laura Feltri, Jun Qu, Thomas Hollis, Jules Berlin Nde Kengne, Bernard Fongang, Rui J. Sousa, Mikhail E. Kandel, Eugene S. Kandel, Klaus M. Hahn, Mikhail A. Nikiforov
Summary: The study found that fluctuating GTP levels around RAC1-GTP Kd correlated with changes in RAC1 activity in live cells, and RAC1 co-localized with several guanylate metabolism enzymes in cell protrusions, revealing a mechanism of RAC1 activity regulation by local GTP pools in live cells.
NATURE COMMUNICATIONS
(2021)
Article
Pharmacology & Pharmacy
Xiaohui Lv, Honghong Zhou, Kai Hu, Ling Lin, Yongqiang Yang, Longjiang Li, Li Tang, Jiayi Huang, Yi Shen, Rong Jiang, Jingyuan Wan, Li Zhang
Summary: Accumulated evidence suggests that metabolic events play a significant role in the progression of various diseases, including liver injury. In this study, the metabolic status of pyruvate and its pharmacological significance in LPS/D-Gal-induced liver injury were investigated in mice. The findings indicate that the decline of pyruvate induced by LPS/D-Gal exposure may be a novel metabolic mechanism underlying the development of fulminant liver injury, and PKM2 activators or pyruvate derivatives may have potential value for pharmacological intervention.
PHARMACOLOGICAL RESEARCH
(2021)
Article
Biology
Sephora Sallis, Felix-Antoine Berube-Simard, Benoit Grondin, Elizabeth Leduc, Fatiha Azouz, Catherine Belanger, Nicolas Pilon
Summary: CHARGE syndrome is primarily caused by CHD7 mutation, a chromatin remodeler-coding gene. FAM172A, a poorly characterized factor, is found to be a binding partner of AGO2 and regulates AGO2 nuclear import. The study highlights the importance of noncanonical nuclear functions of AGO2 and its regulatory mechanisms.
LIFE SCIENCE ALLIANCE
(2023)
Article
Genetics & Heredity
Wenfeng Lu, Yun Dong, Qing Cui, Yuhan Wang, Xiwen Yang, Xiaoyue Cai, Ming Zhang
Summary: This study found that citron kinase (CIT) is highly expressed in esophageal squamous cell carcinoma (ESCC) tissues and knockdown of CIT can inhibit cell proliferation and promote apoptosis, as well as reduce tumorigenicity in vivo. Microarray and IPA analysis revealed that CIT interacts with PRKAA1, SQSTM1, and IL6, and upregulation of PRKAA1 and SQSTM1 and downregulation of IL6 can contribute to ESCC development. This suggests that CIT may serve as a promising therapeutic target for ESCC.
FRONTIERS IN GENETICS
(2021)
Article
Biochemistry & Molecular Biology
Carolina Montenegro-Venegas, Debarpan Guhathakurta, Eneko Pina-Fernandez, Maria Andres-Alonso, Florian Plattner, Eckart D. Gundelfinger, Anna Fejtova
Summary: This study reveals that the protein bassoon organizes the release of neurotransmitters by regulating synaptic phosphorylation and cAMP homeostasis. Synapses lacking bassoon exhibit decreased release competence of synaptic vesicles and increased resting pool of vesicles. Furthermore, the CDK5/PDE4/cAMP signaling pathway plays a crucial role in the regulation of neurotransmitter release.
Article
Biotechnology & Applied Microbiology
Hongyi Li, Yifu Wang, Xinxin Zhao, Zhen Yan, Chao Song, Shuguang Wang
Summary: Biofilm is the main cause of membrane biofouling and microbial corrosion. Inhibiting bacteria adhesion is an efficient strategy for biofilm reduction. This study explores the molecular response and metabolic pathways of Escherichia coli when D-tyrosine is used to inhibit bacterial adhesion. D-tyrosine can significantly inhibit bacterial adhesion by reducing the adhesion force and extracellular protein production, leading to decreased cell hydrophobicity and autonomous cell aggregation.
BIOCHEMICAL ENGINEERING JOURNAL
(2023)
Article
Multidisciplinary Sciences
Christopher R. Donnelly, Changyu Jiang, Amanda S. Andriessen, Kaiyuan Wang, Zilong Wang, Huiping Ding, Junli Zhao, Xin Luo, Michael S. Lee, Yu L. Lei, William Maixner, Mei-Chuan Ko, Ru-Rong Ji
Summary: STING is a critical immune regulator that induces type-I interferons and other cytokines to promote immune-cell-mediated clearance of pathogens and neoplastic cells. STING also plays a role in antitumour immunity and has potential as a target for cancer immunotherapy. Additionally, STING is shown to regulate nociception through IFN-I signaling, leading to pain relief in mice and non-human primates.
Article
Medicine, Research & Experimental
Matheus Felipe da Silva, Luan Vitor Alves de Lima, Liana Martins de Oliveira, Simone Cristine Semprebon, Nayane de Oliveira Silva, Amanda Passuello de Aguiar, Mario Sergio Mantovani
Summary: This study aimed to investigate the antiproliferative activity of Diosgenin (DG) in NCI-H460 lung carcinoma cells and explore the underlying mechanisms of antimitotic and alternative cell death pathways. The results showed that DG reduced cell viability and concentration, increased the frequency of cells with multiple nuclei, and modulated gene expression related to cell cycle and cell death. Furthermore, DG also exhibited inhibitory effects on proliferation recovery and clonogenic growth in tumor spheroids.
Article
Biochemistry & Molecular Biology
Shaoni Mukhopadhyay, Maria E. Amodeo, Amy S. Y. Lee
Summary: Cells respond to stress by reducing protein synthesis and activating gene programs. The non canonical cap-binding protein eIF3d plays a critical role in the integrated stress response by controlling core stress response factors. It activates the kinase GCN2 and upregulates the m6A demethylase ALKBH5 to regulate translation and transcription of stress response genes.
Article
Developmental Biology
Laurine Gagniac, Mariam Rusidze, Frederic Boudou, Stephanie Cagnet, Marine Adlanmerini, Pauline Jeannot, Nicolas Gaide, Frank Giton, Arnaud Besson, Ariane Weyl, Pierre Gourdy, Isabelle Raymond-Letron, Jean-Francois Arnal, Cathrin Brisken, Francoise Lenfant
Article
Multidisciplinary Sciences
Gabrielle Sueur, Alison Boutet, Mathilde Gotanegre, Veronique Mansat-De Mas, Arnaud Besson, Stephane Manenti, Sarah Bertoli
SCIENTIFIC REPORTS
(2020)
Article
Oncology
Maelle Cartel, Pierre-Luc Mouchel, Mathilde Gotanegre, Laure David, Sarah Bertoli, Veronique Mansat-De Mas, Arnaud Besson, Jean-Emmanuel Sarry, Stephane Manenti, Christine Didier
Summary: The research demonstrates that inhibition of deubiquitinylase USP7 reduces cell proliferation, blocks DNA replication, and increases cell death in AML. High expression of USP7 is associated with resistance to therapy in AML patients, indicating USP7 as both a marker of resistance and a potential therapeutic target for overcoming treatment resistance.
Editorial Material
Cell Biology
Ada Nowosad, Arnaud Besson
Article
Cell Biology
Ada Nowosad, Pauline Jeannot, Caroline Callot, Justine Creff, Renaud Thierry Perchey, Carine Joffre, Patrice Codogno, Stephane Manenti, Arnaud Besson
NATURE CELL BIOLOGY
(2020)
Review
Cell Biology
Justine Creff, Arnaud Besson
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Anne Prel, Christine Dozier, Jean-Philippe Combier, Serge Plaza, Arnaud Besson
Summary: Some miRNAs are located in long non-coding RNA precursors, which may potentially encode miPEPs. While plant miPEPs enhance the transcription of their own pri-miRNAs, it is not a general rule for human miPEPs to regulate their own miRNAs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Ada Nowosad, Justine Creff, Pauline Jeannot, Raphael Culerrier, Patrice Codogno, Stephane Manenti, Laurent Nguyen, Arnaud Besson
Summary: In response to glucose starvation, p27 promotes autophagy by facilitating autophagosome trafficking along microtubule tracks via maintaining elevated microtubule acetylation through an ATAT1-dependent mechanism.
CELL DEATH & DISEASE
(2021)
Correction
Cell Biology
Ada Nowosad, Pauline Jeannot, Caroline Callot, Justine Creff, Renaud Thierry Perchey, Carine Joffre, Patrice Codogno, Stephane Manenti, Arnaud Besson
NATURE CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ada Nowosad, Arnaud Besson
Summary: Lysosomes, initially known for their degradative functions, have emerged as signaling hubs that regulate various cellular processes and play a role in stem cell fate regulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Christine Dozier, Audrey Montigny, Mireia Viladrich, Raphael Culerrier, Jean-Philippe Combier, Arnaud Besson, Serge Plaza
Summary: miRNAs are small regulatory non-coding RNAs generated from pri-miRNAs, with some hosted in lncRNAs containing translatable smORFs. This study demonstrates the involvement of smORFs in regulating pri-miRNAs and miRNAs expression in Human and Drosophila, independent of nucleotide and amino acid sequences.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Franck Talmont, Elodie Mitri, Christine Dozier, Arnaud Besson, Olivier Cuvillier, Anastassia Hatzoglou
Summary: Sphingosine 1-phosphate (S1P), a lipid metabolite, interacts with five high-affinity G protein-coupled receptors (S1P1-5) to regulate cell proliferation, survival, and migration. The role of S1P5 in cell proliferation is limited and inconsistent. This study found that unlike other S1P receptors, S1P5 has an anti-proliferative function and promotes cell immortalization and proliferation by controlling the spatial activation of ERK. These findings provide new insights into the anti-proliferative function of S1P5.
Article
Cell Biology
Justine Creff, Ada Nowosad, Anne Prel, Anne Pizzoccaro, Marion Aguirrebengoa, Nicolas Duquesnes, Caroline Callot, Thomas Jungas, Christine Dozier, Arnaud Besson
Summary: p57Kip2 is a cyclin/CDK inhibitor that regulates intestinal stem cell (ISC) fate and proliferation in a CDK-independent manner. In the absence of p57, there is increased proliferation of intestinal crypts and amplification of transit-amplifying cells and Hopx+ ISCs. RNA sequencing analyses reveal significant gene expression changes in Hopx+ ISCs upon p57 depletion. Furthermore, p57 binds to and inhibits the activity of Ascl2, a transcription factor critical for ISC specification and maintenance, by recruiting a corepressor complex. Hence, p57 plays a key role in maintaining Hopx+ ISC quiescence and repressing the ISC phenotype by inhibiting Ascl2 in a CDK-independent manner during intestinal development.
Article
Biochemical Research Methods
Ada Nowosad, Arnaud Besson
Summary: The protocol presented offers a method to monitor starvation-induced autophagy using a live high-throughput microscopy system without the need for sample preparation. By generating turboGFP-LC3B expressing mouse embryonic fibroblasts, autophagy can be measured over time, and data can be analyzed effectively.
Review
Cell & Tissue Engineering
Justine Creff, Laurent Malaquin, Arnaud Besson
Summary: Recent advances in bioengineering have led to the development of various in vitro models to mimic the complex intestinal epithelium for studying fundamental mechanisms and drug screening. These microfabricated systems provide alternative approaches for understanding intestinal homeostasis and pathologies.
JOURNAL OF TISSUE ENGINEERING
(2021)