Article
Food Science & Technology
Huifang Li, Hao He, Chang Liu, Toyosi Akanji, Justin Gutkowski, Ruilian Li, Huiqun Ma, Yinsheng Wan, Panpan Wu, Dongli Li, Navindra P. Seeram, Hang Ma
Summary: The study found that oleuropein and hydroxytyrosol have inhibitory effects on elastase and collagenase at certain concentrations, and their combination can produce synergistic inhibitory effects. In addition, hydroxytyrosol can reduce hydrogen peroxide-induced cytotoxicity and reactive oxygen species levels, and the combination of oleuropein and hydroxytyrosol has synergistic cytoprotective effects on cells.
INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION
(2022)
Article
Biochemistry & Molecular Biology
Joana M. Andrade, Eva Maria Dominguez-Martin, Marisa Nicolai, Celia Faustino, Luis Monteiro Rodrigues, Patricia Rijo
Summary: The study found that extracts from Plectranthus spp. plants have antioxidant and inhibitory effects on skin-related enzymes, with certain isolated compounds showing significant activity against tyrosinase and elastase.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2021)
Article
Food Science & Technology
Chunmei Gu, Qiuping Yang, Shujun Li, Linlin Zhao, Bo Lyu, Yingnan Wang, Hansong Yu
Summary: The bioactive components in soybeans have important physiological functions, but the intake of soybean trypsin inhibitor (STI) may lead to metabolic disorders. In order to investigate the impact of STI intake on pancreatic injury and its mechanism, a five-week animal experiment was conducted, and a weekly monitoring on oxidation degree and antioxidant indexes in serum and pancreas was carried out. The results showed that STI intake caused irreversible damage to the pancreas and induced oxidative stress, resulting in structural damage and dysfunction.
Article
Biophysics
Jiantao Ping, Wenli Wu, Lubin Qi, Jie Liu, Jinpeng Liu, Binglu Zhao, Quanbo Wang, Li Yu, Jin-Ming Lin, Qiongzheng Hu
Summary: The detection of trypsin and its inhibitor using a hydrogel-assisted paper-based lateral flow sensor is demonstrated for the first time. The system has a low detection limit of 1.0 x 10-6 mg/mL, and can also be applied for quantitative detection of trypsin in human serum. It offers a simple, elegant, robust, competitive, high-throughput, and low-cost approach for the rapid and label-free detection, showing great potential for commercial sensing, diagnostic, and pharmaceutical applications.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Multidisciplinary Sciences
Thomas H. Craven, Tashfeen Walton, Ahsan R. Akram, Emma Scholefield, Neil McDonald, Adam D. L. Marshall, Duncan C. Humphries, Bethany Mills, Thane A. Campbell, Annya Bruce, Joanne Mair, James W. Dear, David E. Newby, Adam T. Hill, Timothy S. Walsh, Chris Haslett, Kevin Dhaliwal
Summary: The development of a molecular imaging probe targeting neutrophil activation allowed for real-time identification of neutrophil activity in the lungs of patients, enabling biological stratification based on disease states and severity. Evaluation of the probe's imaging responses in different patient populations confirmed that the optical signal was indeed due to the probe's function and neutrophil activation.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Livia de Moraes Bomediano Camillo, Graziele Cristina Ferreira, Adriana Feliciano Alves Duran, Flavia Ribeiro Santos da Silva, Wanius Garcia, Ana Ligia Scott, Sergio Daishi Sasaki
Summary: rBmTI-A is a recombinant serine protease inhibitor belonging to the Kunitz-BPTI family, showing inhibitory activities against bovine trypsin, human plasma kallikrein, human neutrophil elastase, and plasmin. It has two inhibitory domains with six cysteines each, forming three disulfide bonds for high structural stability. Experimental results suggest its protective potential against pulmonary emphysema, anti-inflammatory properties, and potent inhibitory activity on in vitro vessel formation, while circular dichroism data confirmed its thermostability and secondary structure.
Article
Chemistry, Multidisciplinary
Angelique Rivot, Natacha Jugniot, Samuel Jacoutot, Nicolas Vanthuyne, Philippe Massot, Philippe Mellet, Sylvain R. A. Marque, Gerard Audran, Pierre Voisin, Marie Delles, Gilles Devouassoux, Eric Thiaudiere, Abderrazzak Bentaher, Elodie Parzy
Summary: The study suggests using OMRI in mice to evaluate protease activity in the lungs, finding an increase in proteolysis in LPS-induced inflammation. This preclinical detection method may open new avenues for diagnosis and in vivo antiprotease testing.
Article
Agronomy
Aron Park, Se-Hee Kang, Byeong-Hee Kang, Sreeparna Chowdhury, Seo-Young Shin, Won-Ho Lee, Jeong-Dong Lee, Sungwoo Lee, Yu-Mi Choi, Bo-Keun Ha
Summary: This study evaluated the trypsin inhibitor activity in 999 Korean soybean accessions and conducted molecular characterization of soybean accessions with low activity. A previously unreported mutation in the KTi-1 gene was identified and a marker was developed.
Article
Orthopedics
Suleiman Alhaji Muhammad, Norshariza Nordin, Paisal Hussin, Muhammad Zulfadli Mehat, Sheau Wei Tan, Sharida Fakurazi
Summary: This study compared different protocols for isolating chondrocytes from cartilage and found that using collagenase in HBSS yielded the highest number of viable chondrocytes, which expressed the chondrocyte phenotype.
Review
Biochemistry & Molecular Biology
Rita Favas, Janaina Morone, Rosario Martins, Vitor Vasconcelos, Graciliana Lopes
Summary: Skin, as the largest organ in the human body, acts as a physicochemical barrier protecting against environmental stressors and plays a significant psychosocial role in the aging population. The cosmetic industry is developing new formulas to reduce visible signs of aging prompted by consumer demands for skincare. Increasing importance is placed on natural products, with cyanobacteria and microalgae gaining importance for their anti-aging potential and effects on enzymes involved in pigmentation processes.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2021)
Article
Food Science & Technology
Hani Hafeeza Halim, Mohd Sabri Pak Dek, Azizah Abdul Hamid, Nazamid Saari, Mohd Izwan Mohd Lazim, Faridah Abas, Ahmad Ngalim, Amin Ismail, Ahmad Haniff Jaafar
Summary: This research evaluated the functional properties of young and mature coconut water from the four most popular coconut varieties in Malaysia. It was found that young Malaysian Yellow Dwarf (MYD) had the highest antioxidant and anti-aging activities, making it a potential novel source of natural functional ingredients for skin anti-aging.
Article
Food Science & Technology
Chen Li, Yuhang Han, Liufang Gao, Yi Zhang, Benjamin Kofi Simpson
Summary: The research showed that rEBTI-Sepharose CL-4B could obtain high purity grass carp trypsin through one-step purification, which is less tedious and more cost-effective, meeting the requirements for high-end biotechnological applications in the biomedical and pharmaceutical industries.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lorenza Marinaccio, Azzurra Stefanucci, Giuseppe Scioli, Alice Della Valle, Gokhan Zengin, Angelo Cichelli, Adriano Mollica
Summary: This review provides an overview of the latest advancements in peptide human neutrophil elastase inhibitors isolated from natural sources, including their identification, structures, and mechanisms of action. These inhibitors play a significant role in the development of potent bioactive compounds.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Emergency Medicine
Ian S. DeSouza, Alexander Lipsitt
Summary: Daily, large ingestions of soymilk can potentially lead to acute pancreatitis due to the trypsin inhibitor in soybean, which reduces trypsin activity and can cause inappropriate increase in intrapancreatic trypsin secretion when intestinal proteolytic activity decreases, exceeding the capacity of pancreatic secretory trypsin inhibitor.
AMERICAN JOURNAL OF EMERGENCY MEDICINE
(2021)
Article
Multidisciplinary Sciences
Eunsoo Kim, Zayed Attia, Rachel M. Woodfint, Cong Zeng, Sun Hee Kim, Haley E. Steiner, Rajni Kant Shukla, Namal P. M. Liyanage, Shristi Ghimire, Jianrong Li, Gourapura J. Renukaradhya, Abhay R. Satoskar, Amal O. Amer, Shan-Lu Liu, Estelle Cormet-Boyaka, Prosper N. Boyaka
Summary: The study suggests that inhibiting neutrophil elastase can enhance the efficacy of aluminum-based vaccines by promoting high-affinity antibody production and broader T cell responses, particularly Th1 and Tfh responses. Additionally, suppression of elastase activity can induce mucosal immunity after systemic immunization, and enhance the generation of anti-SARS-CoV-2 antibodies targeting specific epitopes of the spike protein.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biophysics
Alireza Jafarinia, Gian Marco Melito, Thomas Stephan Mueller, Malte Rolf-Pissarczyk, Gerhard A. A. Holzapfel, Guenter Brenn, Katrin Ellermann, Thomas Hochrainer
Summary: The morphological parameters of the dissected aorta, including the false lumen (FL) diameter and the size and location of intimal tears, play a crucial role in the formation of FL thrombosis in Type B aortic dissection (TBAD). When the FL diameter is larger than the true lumen diameter, there is a higher risk of partial thrombosis. Additionally, a higher ratio of distal to proximal tear size increases the risk of FL patency. These parameters are important in classifying the morphology of FL into patent, partially thrombosed, and fully thrombosed.
BIOMECHANICS AND MODELING IN MECHANOBIOLOGY
(2023)
Article
Engineering, Biomedical
Emmanouil Agrafiotis, Christian Mayer, Martin Grabenwoeger, Daniel Zimpfer, Peter Regitnig, Heinrich Maechler, Gerhard A. Holzapfel
Summary: This study examined the effects of thoracic endovascular repair (TEVAR) on the biomechanical properties of the aorta. It found that TEVAR resulted in reduced aortic distensibility, stiffness mismatch, and histological remodeling of the aortic wall. These findings have implications for improving stent-graft design and minimizing complications.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Anna Pukaluk, Heimo Wolinski, Christian Viertler, Peter Regitnig, Gerhard A. Holzapfel, Gerhard Sommer
Summary: This study focuses on the changes in collagen and elastin microstructure in the aortic adventitia under macroscopic equibiaxial loading. The results show that collagen fibers in the adventitia divide into two families under loading, while elastin fibers do not show clear orientation. These findings provide important insights into the stretching process of the aortic wall.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Multidisciplinary
Kevin Linka, Adrian Buganza Tepole, Gerhard A. Holzapfel, Ellen Kuhl
Summary: Choosing the best constitutive model and parameters in continuum mechanics has traditionally relied on user experience and preference. This paper proposes a new method that autonomously discovers the best model and parameters to explain experimental data using a neural network. The method is robust and satisfies physical constraints, and has the potential to revolutionize the field of constitutive modeling. Evaluation: 8 points
COMPUTER METHODS IN APPLIED MECHANICS AND ENGINEERING
(2023)
Article
Engineering, Multidisciplinary
Will Zhang, Javiera Jilberto, Gerhard Sommer, Michael S. Sacks, Gerhard A. Holzapfel, David A. Nordsletten
Summary: Biomechanics is important in diagnosing and treating heart conditions. Computational models can provide personalized treatment options but require accurate constitutive equations for biomechanical behavior prediction. A fractional viscoelastic modeling approach that accurately captures the viscoelastic response of the human myocardium was previously developed. This approach has comparable computational costs and only requires two additional material parameters. In this study, the implementation of this approach in Finite Element Analysis was presented, numerical properties were examined, and the physiological implications were explored.
COMPUTER METHODS IN APPLIED MECHANICS AND ENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Manuel P. Kainz, Alexander Greiner, Jan Hinrichsen, Dagmar Kolb, Ester Comellas, Paul Steinmann, Silvia Budday, Michele Terzano, Gerhard A. Holzapfel
Summary: A novel nonlinear poro-viscoelastic computational model based on the Theory of Porous Media was proposed to describe the mechanical response of brain tissue. Indentation experiments were conducted on a tailor-made hydrogel mimicking brain tissue, and an inverse parameter identification scheme was used to extract the optimal constitutive model parameters. The model was validated through finite element simulations.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Cardiac & Cardiovascular Systems
Colton J. Ross, Arshid Mir, Harold M. Burkhart, Gerhard A. Holzapfel, Chung-Hao Lee
Summary: Hypoplastic Left Heart Syndrome (HLHS) is a congenital heart defect that requires a three-stage surgical palliation. Tricuspid regurgitation (TR) occurs in 25% of patients undergoing this procedure and is associated with increased mortality risk. Valvular anomalies and geometric properties are identified as the main predictors for poor prognosis in HLHS patients with TR. Future studies should focus on engineering-based metrics, multivariate analysis, and predictive models to determine the predictors of TR onset during the three palliation stages. These efforts will lead to the development of innovative tools for surgical timing decisions, prophylactic surgical valve repair, and improvement of current intervention techniques.
JOURNAL OF CARDIOVASCULAR DEVELOPMENT AND DISEASE
(2023)
Article
Engineering, Biomedical
Will Zhang, Majid Jadidi, Sayed Ahmadreza Razian, Gerhard A. Holzapfel, Alexey Kamenskiy, David A. Nordsletten
Summary: This study introduces a new viscoelastic constitutive model for the human femoropopliteal artery, which accurately describes its mechanical characteristics and time-dependent behavior. The model improves the accuracy of computational simulations and expands our knowledge of arterial mechanophysiology.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Federica Cosentino, Selda Sherifova, Gerhard Sommer, Giuseppe Raffa, Michele Pilato, Salvatore Pasta, Gerhard A. Holzapfel
Summary: This study investigates regional differences in ascending thoracic aortic aneurysms (ATAAs) in humans, highlighting the impact of fiber dispersion on microstructural properties and providing important biomechanical data for improving current risk stratification strategies.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Mohammad Javad Sadeghinia, Hans Martin Aguilera, Stig Urheim, Robert Matongo Persson, Vegard Skalstad Ellensen, Rune Haaverstad, Gerhard A. Holzapfel, Bjorn Skallerud, Victorien Prot
Summary: This study examines the mechanical behavior of Barlow and FED tissue by using biaxial mechanical tests and second harmonic generation microscopy. The results show that the Barlow sample and the most affected FED sample have different mechanical behavior and collagen structure compared to the other FED samples. The finite element model constructed based on this study shows good agreement with echocardiography and provides essential data for understanding the relationship between collagen microstructure and degenerative mitral valve mechanics.
ACTA BIOMATERIALIA
(2023)
Article
Materials Science, Multidisciplinary
Maximilian P. Wollner, Michele Terzano, Malte Rolf-Pissarczyk, Gerhard A. Holzapfel
Summary: This article develops a material model capable of reproducing anisotropy, viscoelasticity, stress softening, and permanent set by merging several pre-existing frameworks. Each constitutive effect is discussed separately in terms of its thermodynamics and mechanical interpretation and successively built on top of each other. The pseudo-elastic approach to permanent set occupies a special place in the formulation, with a novel discussion of its applicability to generic deformations. The capabilities and possible shortcomings of the formulation are highlighted and a simple numerical algorithm for stress computation is presented.
JOURNAL OF THE MECHANICS AND PHYSICS OF SOLIDS
(2023)
Article
Multidisciplinary Sciences
Misael Dalbosco, Michele Terzano, Thiago A. Carniel, Eduardo A. Fancello, Gerhard A. Holzapfel
Summary: This study presents a multi-scale computational analysis of abdominal aortic aneurysms (AAAs), which suggests that the formation of aneurysms disrupts the micro-mechanics of healthy tissue and triggers collagen growth and remodeling through mechanosensing cells. This leads to changes in the macro-mechanics and microstructure of the tissue at a cellular scale.
JOURNAL OF THE ROYAL SOCIETY INTERFACE
(2023)
Article
Multidisciplinary Sciences
Michele Terzano, Maximilian P. Wollner, Manuel P. Kainz, Malte Rolf-Pissarczyk, Nils Gotzen, Gerhard A. Holzapfel
Summary: In situ tissue engineering provides a innovative solution for replacing valves and grafts in cardiovascular medicine. The mechanical behavior of the polymeric scaffold and its short-term response are studied using simulations and experiments.
JOURNAL OF THE ROYAL SOCIETY INTERFACE
(2023)