Article
Endocrinology & Metabolism
Pavel Hruska, Jan Kucera, Matej Pekar, Pavol Holeczy, Miloslav Mazur, Marek Buzga, Daniela Kuruczova, Peter Lenart, Jana Fialova Kucerova, David Potesil, Zbynek Zdrahal, Julie Bienertova-Vasku
Summary: The study compared the proteomic profiles of mature adipocytes from different adipose tissue depots, with findings showing that subcutaneous adipocytes are more active in vesicular transport, secretion, and lipid metabolism, while visceral adipocytes have higher protein expression related to mitochondrial energy metabolism and translational or biosynthetic activity.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2022)
Article
Cell Biology
Isis Cortes, Gutemberg Alves, Cesar Claudio-Da-Silva, Leandra Santos Baptista
Summary: This study found that different subpopulations of adipose stem/stromal cells (ASCs) exhibit distinct physiological properties in a 3D spheroid culture. ASC spheroids from superficial subcutaneous adipose tissue (sSAT) showed the highest response to adipogenic induction, while spheroids from deep subcutaneous adipose tissue (dSAT) showed the greatest lipolytic capacity. Spheroids from superficial retinacula cutis (sRC) exhibited a greater secretory capacity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Genetics & Heredity
Linghui Wang, Hao Gu, Tianci Liao, Yuhang Lei, Yanhao Qiu, Qiuyang Chen, Lei Chen, Shunhua Zhang, Jinyong Wang, Xiaoxia Hao, Dongmei Jiang, Ye Zhao, Lili Niu, Xuewei Li, Linyuan Shen, Mailin Gan, Li Zhu
Summary: By sequencing, identifying, and analyzing tsRNAs using pigs as animal models, this research reports the characteristics of tsRNAs in subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT). A total of 474 tsRNAs, with differential expression in endocrine and immune systems, as well as lipid metabolism, were found in white adipose tissue (WAT). The study also discovered a connection between the activity of the host tRNA engaged in translation and the production of tsRNAs. Furthermore, tsRNAs and miRNAs were found to be involved in the regulation of fatty acid metabolism in adipose tissue.
Article
Biochemistry & Molecular Biology
Farrukh Aqil, Jeyaprakash Jeyabalan, Radha Munagala, Iqbal Ahmad, David J. Schultz, Ramesh C. Gupta
Summary: Dietary supplementation with cumin powder and extract can significantly delay and prevent E2-mediated mammary tumorigenesis, reduce tumor volume and multiplicity, and reverse the effects of E2 on pituitary growth factors and miRNAs in mammary tissues.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Oscar Osorio-Conles, Arturo Vega-Beyhart, Ainitze Ibarzabal, Jose Maria Balibrea, Josep Vidal, Ana de Hollanda
Summary: The metabolic syndrome is a cluster of dangerous heart attack risk factors. This study compares the dysfunction of obesity-associated white adipose tissue (WAT) between women with severe obesity with or without metabolic syndrome. The results show that women with metabolic syndrome exhibit greater adipocyte hypertrophy, increased fibrosis, and higher expression of macrophage and hypoxia markers in subcutaneous adipose tissue.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Geriatrics & Gerontology
Menghua Zhu, Hongyu Li, Yue Yin, Min Ding, Cyriac Abby Philips, Fernando Gomes Romeiro, Xingshun Qi
Summary: Both excessive deficiency and accumulation of subcutaneous adipose tissues negatively influence the outcomes of cirrhotic patients.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2023)
Article
Biochemistry & Molecular Biology
Francesca Favaretto, Chiara Compagnin, Elisa Cogliati, Giulia Montagner, Francesco Dell'Antonia, Giorgio Berna, Roberto Vettor, Gabriella Milan, Diletta Trojan
Summary: This study aimed to characterize and validate the storage effectiveness of cryopreserved adipose tissue (AT) for up to three years for clinical applications. AT components were isolated from fresh and cryopreserved samples and cell viability was assessed. Adipogenic and endothelial precursors and mature cells were measured. The differentiation potential and cellular composition of cryopreserved AT were maintained. Two patients successfully underwent autologous cryopreserved AT transplants without complications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Jose Segura, Ana Isabel Rey, Alvaro Olivares, Maria Isabel Cambero, Rosa Escudero, Maria Dolores Romero dede de Avila, Antonio Palomo, Clemente Lopez-Bote
Summary: The study investigates the impact of different diets on fatty acid composition and textural properties of subcutaneous fat in heavy Iberian pigs. Moderate oleic acid enrichment does not significantly affect triglyceride regulation, while high oleic acid intake from acorns alters fatty acid proportions. Iberian pigs raised in free-range conditions exhibit a more beneficial lipid profile for human health compared to those fed conventional diets.
Article
Endocrinology & Metabolism
Darcy Kahn, Emily Macias, Simona Zarini, Amanda Garfield, Karin Zemski Berry, Paul MacLean, Robert E. Gerszten, Andrew Libby, Claudia Solt, Jonathan Schoen, Bryan C. Bergman
Summary: Research has found that the secretions of adipose tissue vary based on anatomical location and affect insulin responsiveness through paracrine and endocrine signaling. Visceral adipose tissue secretes more cytokines and adipokines than subcutaneous adipose tissue, leading to a negative impact on insulin action in the liver and muscles.
Article
Endocrinology & Metabolism
Mohamed Fouad Mansour, Sophie Blanchette, Melissa Pelletier, Donald Poirier, Andre Tchernof
Summary: 17 beta-hydroxysteroid dehydrogenase type 2 (17β-HSD2) plays an important role in the regulation of estradiol (E2) availability in adipose tissues by converting E2 into estrone (E1). This study found significant conversion of E2 into E1 in omental (OM) and subcutaneous (SC) adipose tissues of women, with higher expression and activity of 17β-HSD2 in OM compared to SC adipose tissue. Additionally, the activity of this enzyme was negatively associated with body mass index, waist circumference, lean body mass, and SC adipose tissue areas.
CLINICAL ENDOCRINOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Alexander J. Knights, Shanshan Liu, Yingxu Ma, Victoria S. Nudell, Eric Perkey, Matthew J. Sorensen, Robert T. Kennedy, Ivan Maillard, Li Ye, Heejin Jun, Jun Wu
Summary: This study demonstrates that macrophages play a crucial role in regulating thermogenesis by secreting acetylcholine in adipose tissue. The abundance of ChAMs increases in response to acute cold exposure, and their regulation is mediated through adrenergic signaling. Deletion of the enzyme ChAT impairs cold-induced thermogenesis in mice.
Article
Biochemistry & Molecular Biology
Jesus Fernandez-Felipe, Adrian Plaza, Gema Dominguez, Javier Perez-Castells, Victoria Cano, Francesco Cioni, Nuria Del Olmo, Mariano Ruiz-Gayo, Beatriz Merino
Summary: High-fat diets enriched with lauric acid do not increase leptin production and reduce the effect of leptin on kinase phosphorylation. Both high-fat diets affect adipocyte-size distribution and gene expression related to adipogenesis and inflammation. The composition of white adipose tissue is different between the two diets, which may impact the biophysical properties and signaling of leptin receptor.
Article
Multidisciplinary Sciences
Kirstin A. MacGregor, Nidia Rodriguez-Sanchez, Nicholas D. Barwell, Iain J. Gallagher, Colin N. Moran, Thomas G. Di Virgilio
Summary: Studying human subcutaneous adipose tissue is important for understanding metabolic conditions. Through proper training and adherence to aseptic techniques, researchers can safely and efficiently obtain adipose tissue samples. The biopsy technique is safe for both participants and research staff in non-clinical settings.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Nutrition & Dietetics
Tiziana Zotti, Antonia Giacco, Arianna Cuomo, Luigi Cerulo, Giuseppe Petito, Stefania Iervolino, Rosalba Senese, Federica Cioffi, Pasquale Vito, Gaetano Cardinale, Elena Silvestri, Assunta Lombardi, Maria Moreno, Antonia Lanni, Pieter de Lange
Summary: Combining exercise with fasting can increase the release of fatty acids from adipose tissues, providing fuel for surrounding organs. This study compared the effect of fasting and exercise on fatty acid composition in different adipose tissue depots, and found that exercise combined with fasting led to increased fatty acid mobilization from visceral adipose tissue.
Article
Oncology
Zhihao Jia, Xiyue Chen, Jingjuan Chen, Lijia Zhang, Stephanie N. Oprescu, Nanjian Luo, Yan Xiong, Feng Yue, Shihuan Kuang
Summary: This study identifies ACSS3 as the key enzyme for propionate metabolism and demonstrates that accumulation of propionate promotes obesity and Type 2 diabetes through triggering adipocyte autophagy.
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Vesa M. Olkkonen, Elina Ikonen
Summary: This review summarizes the role of OSBP family members in facilitating cholesterol trafficking within the endocytic pathway, particularly at membrane contact sites within the cell. ORP1 isoforms have been shown to mediate cholesterol transport between the endoplasmic reticulum and late endosomes/lysosomes, while ORP2 plays a role in transferring cholesterol between late endosomes and recycling endosomes. These processes involve lipid exchange and contribute to regulating cellular signaling pathways.
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2022)
Article
Cell Biology
Yoann Combot, Veijo T. Salo, Gilliane Chadeuf, Maarit Holtta, Katharina Ven, Ilari Pulli, Simon Ducheix, Claire Pecqueur, Ophelie Renoult, Behnam Lak, Shiqian Li, Leena Karhinen, Ilya Belevich, Cedric Le May, Jennifer Rieusset, Soazig Le Lay, Mikael Croyal, Karim Si Tayeb, Helena Vihinen, Eija Jokitalo, Kid Tornquist, Corinne Vigouroux, Bertrand Cariou, Jocelyne Magre, Abdelhalim Larhlimi, Elina Ikonen, Xavier Prieur
Summary: Deficiency of seipin, an ER protein, leads to mitochondrial abnormalities and dysfunctions in adipose tissue. Seipin controls mitochondrial energy metabolism by regulating mitochondrial calcium influx at ER-mitochondria contact sites (MAMs). Reduced ATP production in seipin-deficient adipose tissue contributes to the development of lipodystrophy.
Article
Urology & Nephrology
Anna-Maija Itkonen Freitas, Camilla Isaksson, Paivi Rahkola-Soisalo, Sari Tulokas, Maarit Mentula, Tomi S. Mikkola
Summary: This study compared the efficacy of polyacrylamide hydrogel (PAHG) and tension-free vaginal tape (TVT) in the treatment of primary stress urinary incontinence (SUI). The results showed that PAHG did not meet the noninferiority criteria set in the study, and TVT slings had better subjective and objective cure rates but were associated with more complications. Therefore, PAHG can be offered as a safe and durable alternative treatment for women with primary SUI.
JOURNAL OF UROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Alexandra Robciuc, Hanna Savolainen-Peltonen, Mikko Haanpaa, Jukka A. O. Moilanen, Tomi S. Mikkola
Summary: The sexual dimorphism in inflammatory/autoimmune diseases is mainly caused by sex hormone actions. Dry eye, a common eye disease, disproportionately affects women. The hormone levels in tears are correlated with serum levels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, General & Internal
Minttu Venetkoski, Johanna Joensuu, Mika Gissler, Olavi Ylikorkala, Tomi Sakari Mikkola, Hanna Savolainen-Peltonen
Summary: Pre-eclampsia has a significant impact on the future cardiovascular risk of Finnish women, with elevated risks for ischemic heart disease, myocardial infarction, and stroke among women with a history of pre-eclampsia.
Article
Psychology, Clinical
Nico Vehmas, Elina Holopainen, Laura Suomalainen, Hanna Savolainen-Peltonen
Summary: The study found that Finnish adolescents seeking GAHT for gender dysphoria have good somatic health, but a higher proportion of overweight, depression, and anxiety compared to the general adolescent population.
TRANSGENDER HEALTH
(2022)
Article
Cell Biology
Elina Ikonen, Vesa M. Olkkonen
Summary: Cholesterol is acquired by cells through synthesis in the ER and uptake from lipoproteins. It is distributed to various organelles through lipid-binding proteins at MCSs, and is also transported through vesicle/tubule-mediated transport. This review provides an overview of intracellular cholesterol trafficking pathways, as well as the diseases and therapeutic strategies associated with defects in these processes.
COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Calvin Dumesnil, Lauri Vanharanta, Xavier Prasanna, Mohyeddine Omrane, Maxime Carpentier, Apoorva Bhapkar, Giray Enkavi, Veijo T. T. Salo, Ilpo Vattulainen, Elina Ikonen, Abdou Rachid Thiam
Summary: Cellular cholesterol can be metabolized into cholesteryl esters (CEs) and stored in lipid droplets (LDs). The formation of CE-rich LDs in cells is achieved through supercooled droplets and liquid-crystalline phases, which are influenced by the concentration of CEs and the presence of triacylglycerols (TGs).
NATURE COMMUNICATIONS
(2023)
Article
Obstetrics & Gynecology
M. Venetkoski, O. Ylikorkala, J. M. Joensuu, M. Gissler, T. S. Mikkola, H. Savolainen-Peltonen
Summary: This study compared the trends of hormone therapy (HT) use between women with and without a history of pre-eclampsia. The results showed that the use of HT increased in women with a history of pre-eclampsia during the later half of the follow-up period, possibly due to changes in international recommendations, increased awareness of pre-eclampsia-related cardiovascular risk later in life, and the aim to reduce this risk through HT.
Article
Multidisciplinary Sciences
Viola Nahse, Camilla Raiborg, Kia Wee Tan, Sissel Mork, Maria Lyngaas Torgersen, Eva Maria Wenzel, Mireia Nager, Veijo T. T. Salo, Terje Johansen, Elina Ikonen, Kay Oliver Schink, Harald Stenmark
Summary: DFCP1 is an ATPase located on omegasomes and plays a crucial role in selective autophagy by promoting the constriction of large omegasomes. It is essential for maintaining cellular homeostasis by removing damaged organelles and protein aggregates.
NATURE COMMUNICATIONS
(2023)
Article
Biochemical Research Methods
Simon G. Pfisterer, Ivonne Brock, Kristiina Kanerva, Iryna Hlushchenko, Lassi Paavolainen, Pietari Ripatti, Mohammad Majharul Islam, Aija Kyttala, Maria D. Di Taranto, Annalisa Scotto di Frega, Giuliana Fortunato, Johanna Kuusisto, Peter Horvath, Samuli Ripatti, Markku Laakso, Elina Ikonen
Summary: A multiparametric high-content imaging platform is presented for quantifying LDL uptake and lipid storage in primary leukocyte subpopulations, providing systematic insight into cellular dysfunction. Integration of lipid storage data with lipid mobilization improves differentiation of individuals with normal and elevated LDL-c. This platform has broad applications in understanding metabolic disorders.
CELL REPORTS METHODS
(2022)
Article
Polymer Science
Rubina Ajdary, Guillermo Reyes, Jani Kuula, Eija Raussi-Lehto, Tomi S. Mikkola, Esko Kankuri, Orlando J. Rojas
Summary: Direct ink writing is used to synthesize layer-by-layer meshes using renewable polysaccharides, which demonstrate excellent mechanical performance, wet stability, and biocompatibility, making them suitable for biomedical implants.
