Article
Biochemistry & Molecular Biology
Rita Martin-Ramirez, Rebeca Gonzalez-Fernandez, Deborah Rotoli, Jairo Hernandez, Pablo Martin-Vasallo, Angela Palumbo, Julio Avila
Summary: This study found that oxidative stress induced by peroxynitrite affects gene expression in granulosa-lutein cells, while pretreatment with the antioxidant celastrol can prevent this effect. Additionally, celastrol may have an independent role in regulating gene expression in hGL cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Reproductive Biology
Lei Gao, Lutong Zhang, Yuli Zhang, Mielie Madaniyati, Shengjie Shi, Liang Huang, Xiangrong Song, Weijun Pang, Guiyan Chu, Gongshe Yang
Summary: miR-10a-5p regulates steroid hormone synthesis in porcine granulosa cells by inhibiting the expression of key enzymes and modulating cholesterol metabolism pathway.
Article
Agriculture, Dairy & Animal Science
Liang Huang, Huan Yuan, Shengjie Shi, Xiangrong Song, Lutong Zhang, Xiaoge Zhou, Lei Gao, Weijun Pang, Gongshe Yang, Guiyan Chu
Summary: The CLOCK protein was found to inhibit the proliferation of porcine ovarian granulosa cells by increasing ASB9 level.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Rita Martin-Ramirez, Rebeca Gonzalez-Fernandez, Jairo Hernandez, Pablo Martin-Vasallo, Angela Palumbo, Julio avila
Summary: This study demonstrates that celastrol and melatonin can enhance cell survival under oxidative stress conditions. Melatonin protects cells by inducing SIRT1, SIRT6 and SIRT7 gene expression, while celastrol mainly acts through inducing SIRT7 gene expression.
Article
Biochemistry & Molecular Biology
Chenfeng Yuan, Zheng Li, Yun Zhao, Xin Wang, Lu Chen, Zijiao Zhao, Maosheng Cao, Tong Chen, Tariq Iqbal, Boqi Zhang, Wenjing Fan, Yameng Wei, Chunjin Li, Xu Zhou
Summary: This study demonstrated that exosomes play a crucial role in regulating the physiological function of granulosa cells by carrying specific microRNAs, promoting cell proliferation and progesterone synthesis. The MAPK/ERK and WNT/B-CATENIN pathways were found to be involved in these processes.
Article
Obstetrics & Gynecology
Xiao Xu, Hao-Ran Shen, Min Yu, Mei-Rong Du, Xue-Lian Li
Summary: The study revealed that let-7i is down-regulated in PCOS granulosa-luteal cells, and overexpression of let-7i inhibits cell proliferation and decreases estradiol production through IMP2-dependent mechanisms. This finding provides a new molecular mechanism for PCOS.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Article
Reproductive Biology
Hao Yu, Jie Zhao, Xiaomeng Pei, Yuting Xia, Haolin Li, Wei Wang, Dagan Mao
Summary: This study aimed to investigate the role of NR4A1 in forskolin-induced granulosa cell differentiation and PGF2a-induced granulosa-lutein cell regression. The results showed that NR4A1 was up-regulated during granulosa cell differentiation and played a role in cell proliferation, apoptosis, steroidogenesis, and MAPK pathways. Furthermore, NR4A1 was also involved in the regression of granulosa-lutein cells and influenced several cellular processes. These findings provide a theoretical basis for further understanding the mechanism of porcine luteal formation and regression.
Article
Obstetrics & Gynecology
Krutika Patil, Indira Hinduja, Srabani Mukherjee
Summary: In women with PCOS, both follicular fluid (FF) and granulosa-lutein cells (GLCs) exhibit reduced expression of pro-angiogenic factors compared to controls, leading to diminished capacity to induce angiogenesis and potentially contributing to corpus luteum (CL) defects.
HUMAN REPRODUCTION
(2021)
Article
Reproductive Biology
Liguang Wang, Jingjing Li, Lutong Zhang, Shengjie Shi, Xiaoge Zhou, Yamei Hu, Lei Gao, Gongshe Yang, Weijun Pang, Huatao Chen, Lijia Zhao, Guiyan Chu, Chuanjiang Cai
Summary: The transcriptional repressor NR1D1 plays an important role in the circadian system and affects estrogen synthesis. Activation of NR1D1 inhibits the transcriptional activity of CYP19A1, leading to a decrease in estradiol content. This suggests that ovarian hormone secretion is regulated by the biological clock.
Article
Reproductive Biology
Qinyao Wei, Juan Li, Haorong He, Yuchen Cao, Dongmei Li, Felix Kwame Amevor, Yao Zhang, Jianping Wang, Chunlin Yu, Chaowu Yang, Huarui Du, Xiaosong Jiang, Qing Zhu, Huadong Yin
Summary: miR-23b-3p plays a role in regulating the proliferation and steroid hormone synthesis of chicken granulosa cells by inhibiting the expression of GDF9. Elevated expression of miR-23b-3p significantly inhibits granulosa cell proliferation and steroid hormone synthesis without affecting apoptosis. It was found that GDF9 is a target gene of miR-23b-3p and miR-23b-3p can down-regulate its expression.
Article
Agriculture, Dairy & Animal Science
Mingxia Ran, Shenqiang Hu, Qingyuan Ouyang, Hengli Xie, Xi Zhang, Yueyue Lin, Xuejian Li, Jiwei Hu, Liang Li, Hua He, Hehe Liu, Jiwen Wang
Summary: This study investigated the effects of miR-202-5p, which undergoes expression changes during follicular development, on lipid metabolism and steroidogenesis in goose hierarchical follicular granulosa cells. The results showed that miR-202-5p significantly inhibited lipid deposition and steroid hormone production in goose granulosa cells via ACSL3. These findings highlight the important role of miR-202-5p in regulating granulosa cell functions and its potential as a target for molecular breeding.
Article
Physiology
Mitchell Masterson, Riyan Bittar, Hannah Chu, Naoki Yamanaka, Sachiko Haga-Yamanaka
Summary: The study established an assay system to assess the entry of insect steroid hormones into cells and found that ecdysteroids are incorporated through the membrane transporter EcI, providing a novel target for manipulating insect steroid hormone signaling.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Cell Biology
Yuanyuan Hou, Jianing Hu, Jie Li, Hu Li, Yangqing Lu, Xingting Liu
Summary: The study aimed to investigate the role of MFN2 in the proliferation and progesterone biosynthesis of granulosa cells (GCs) during follicle selection in hens. The results showed that the expression level of MFN2 in GCs increased significantly after follicle selection, and it promoted P4 biosynthesis, ATP production, mtDNA copy numbers, and cell proliferation. FSH treatment enhanced these effects in F5G, but not in SYFG. Additionally, overexpression of MFN2 increased P4 biosynthesis, ATP production, mtDNA copy numbers, and cell proliferation in SYFG. These findings suggest that MFN2 upregulation may play a crucial role in the initiation of P4 biosynthesis and the promotion of GCs proliferation during follicle selection.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Environmental Sciences
Ruhi Kabakci, Kendra L. Clark, Michele R. Plewes, Corrine F. Monaco, John S. Davis
Summary: The study investigated the effects of PFOA on bovine granulosa cell function. Results showed that PFOA inhibited steroid hormone secretion and altered the expression of key enzymes required for steroidogenesis. PFOA also caused mitochondrial dysfunction and increased autophagy. These findings suggest that PFOA may negatively impact livestock reproductive health.
ENVIRONMENTAL POLLUTION
(2023)
Article
Agriculture, Dairy & Animal Science
Xing Wu, Na Zhang, Jing Li, Zihao Zhang, Yulong Guo, Donghua Li, Yanhua Zhang, Yujie Gong, Ruirui Jiang, Hong Li, Guoxi Li, Xiaojun Liu, Xiangtao Kang, Yadong Tian
Summary: This study aimed to investigate the regulatory effect of gga-miR-449b-5p on granulosa cell (GC) proliferation and steroidogenesis in laying hens. The results showed that gga-miR-449b-5p had no effect on GC proliferation but regulated the expression of steroid synthesis-related genes and the secretion of P4 and E2. Additionally, gga-miR-449b-5p targeted IGF2BP3 and downregulated its mRNA and protein expression. Overall, gga-miR-449b-5p plays an important role in the synthesis of steroid hormones in laying hen GCs.
Review
Psychiatry
Graziano Grugni, Maria Rosaria Licenziati, Giuliana Valerio, Antonino Crino, Claudio Maffeis, Rita Tanas, Giuseppe Stefano Morino
EATING AND WEIGHT DISORDERS-STUDIES ON ANOREXIA BULIMIA AND OBESITY
(2017)
Article
Endocrinology & Metabolism
Luisa Montanini, Arianna Smerieri, Mariolina Gulli, Francesca Cirillo, Giovanna Pisi, Chiara Sartori, Sergio Amarri, Sergio Bernasconi, Nelson Marmiroli, Maria E. Street
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2016)
Article
Cell Biology
Chiara Sartori, Pietro Lazzeroni, Silvia Merli, Viviana Dora Patianna, Francesca Viaroli, Francesca Cirillo, Sergio Amarri, Maria Elisabeth Street
MEDIATORS OF INFLAMMATION
(2016)
Review
Biochemistry & Molecular Biology
Francesca Cirillo, Pietro Lazzeroni, Chiara Sartori, Maria Elisabeth Street
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2017)
Review
Biochemistry & Molecular Biology
Francesca Cirillo, Pietro Lazzeroni, Cecilia Catellani, Chiara Sartori, Sergio Amarri, Maria Elisabeth Street
CYTOKINE & GROWTH FACTOR REVIEWS
(2018)
Review
Genetics & Heredity
Ilenia Maini, Ivan Ivanovski, Alessandro Lodice, Simonetta Rosato, Marzia Pollazzon, Manuela Mussini, Elga F. Belligni, Charles Coutton, Maria Marinelli, Veronica Barbieri, Manuela Napoli, Rosario Pascarella, Chiara Sartori, Francesca Madia, Carlo Fusco, Fabrizia Franchi, Maria E. Street, Livia Garavelli
MOLECULAR SYNDROMOLOGY
(2016)
Article
Endocrinology & Metabolism
A. Smerieri, M. Petraroli, L. Montanini, C. Sartori, S. Bernasconi, M. E. Street
JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS
(2012)
Article
Endocrinology & Metabolism
M. E. Street, C. Spaggiari, M. A. Ziveri, M. Rossi, C. Volta, I. Viani, G. L. Grzincich, C. Sartori, M. Zanzucchi, V. Raia, C. Terzi, G. Pisi, E. Zanetti, M. C. S. Boguszewski, T. O. Kamoi, S. Bernasconi
JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION
(2012)