Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 945, Issue -, Pages 185-192Publisher
ELSEVIER
DOI: 10.1016/j.jchromb.2013.11.050
Keywords
Imperatorin; Xanthotoxol; Hollow fiber liquid phase microextraction; Pharmacokinetics; HPLC-ESI-MS
Funding
- National Natural Science Foundation of China [81102412]
- Ministry of Education Key Project of Science and Technology Foundation of China [211021]
- Natural Science Foundation of Hebei Province of China [C2011206158, 088031]
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The objective of the present study was to develop a new method for the simultaneous quantitation of imperatorin and its metabolite xanthotoxol in rat plasma. The samples were prepared with hollow fiber liquid phase microextraction (HF-LPME). The optimized extraction procedure was acquired by assessing extraction solvent, length of the fiber, agitation rate, extraction temperature and time. A comparison of sample pretreatment ways between HF-LPME and deproteinization with methanol was performed, which demonstrated less ion suppression and better sensitivity of HF-LPME. Analytes were separated on a C-18 column with a gradient elution consisted of methanol and water containing 1 mmol/L ammonium acetate. The detection was accomplished by electrospray ionization (ESI) source operating in the positive ionization mode. Selected-multiple-reaction monitoring (SMRM) scanning was employed, which guaranteed a higher sensitivity compared with MRM mode. Calibration curves were linear over investigated ranges with correlation coefficients greater than 0.9979. Precision varied from 0.26% to 14%, and the accuracy varied within +/- 5.5%. The developed method was successfully applied to the pharmacokinetic research of imperatorin and its metabolite xanthotoxol after oral administration of imperatorin to rats. (C) 2013 Elsevier B.V. All rights reserved.
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