Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 944, Issue -, Pages 114-122Publisher
ELSEVIER
DOI: 10.1016/j.jchromb.2013.10.037
Keywords
Lumefantrine; Desbutyl lumefantrine; RP-HPLC/UV; Validation
Funding
- University of Peshawar
Ask authors/readers for more resources
A simple, specific, precise and rapid RP-HPLC-UV method was developed for simultaneous determination of lumefantrine and its metabolite desbutyl lumefantrine in human plasma. Experimental parameters were optimized and the method was validated according to standard guidelines. The method showed adequate separation for lumefantrine and desbutyl lumefantrine and best resolution was achieved with Supelco Discovery HS C18 RP (150 mm x 4.6 mm, 5 mu m) column using acetonitrile and 0.05% trifluroacetic acid (70:30, v/v) as a mobile phase pumped at a flow rate of 1.0 ml/min and wavelength of 335 nm. The method was linear over the concentration range of 10-12,000 ng/ml. The lower limit of detection (LLOD) and lower limit of quantification (LLOQ) for lumefantrine were 10.0 and 18.0 ng/ml, while for desbutyl lumefantrine were 7.5 and 15.0 ng/ml, respectively. The proposed method was efficiently applied for determination of lumefantrine and desbutyl lumefantrine concentrations in plasma samples for pharmacokinetic studies. (C) 2013 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available