Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 915, Issue -, Pages 52-56Publisher
ELSEVIER
DOI: 10.1016/j.jchromb.2012.12.014
Keywords
Ecdysteroid biosynthesis; LC-MS/MS; Multiple reaction monitoring (MRM); Silkworm prothoracic glands and hemolymph; Simultaneous quantification
Funding
- Programme for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry
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The concentration changes of endogenous ecdysteroids are closely related to the regulation of insect growth and development. Although they are frequently measured by immunoassays with anti-steroid antibodies, the separate estimations of the individual concentrations of ecdysone and other ecdysteroids with similar chemical structures are quite difficult to accomplish. In this study, an efficient method for the simultaneous, individual quantification of intermediate steroids in ecdysone biosynthesis was developed, using LC-MS/MS. By employing multiple reaction monitoring (MRM) in the MS detection, the selectivity and sensitivity of the method were greatly enhanced, allowing the estimation of trace amounts of steroids in biological samples from silkworm prothoracic glands and hemolymph. (C) 2012 Elsevier B.V. All rights reserved.
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