Article
Chemistry, Multidisciplinary
Limin Hou, Zongpei Jiang, Minqi Ye, Xueyan Sun, Kexin Liu, Yifan Zhu, Xiaoyu Wang, Liangzhu Chen, Ruiheng Gu, Binghu Fang
Summary: The excessive accumulation of antibiotic residue in animal-derived foods poses a threat to health. Therefore, it is necessary to establish a rapid, simple, effective, safe, and highly sensitive method for detecting lincomycin residues. By using a pre-column derivatization method on high-performance liquid chromatography equipped with ultraviolet, the UV absorption of lincomycin is improved, allowing for detection and quantification.
ARABIAN JOURNAL OF CHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Kyong-Oh Shin, Kyungho Park
Summary: Sulforaphane, a naturally occurring isothiocyanate with anti-carcinogenic properties, was quantified using an improved analytical method involving HPLC-UV/Vis. The pharmacokinetic study after oral administration revealed promising results, indicating the method's reliability and applicability for quantifying SFN in biological samples.
Review
Biochemistry & Molecular Biology
Fatima Hameedat, Sahar Hawamdeh, Soraya Alnabulsi, Aref Zayed
Summary: This review presents the HPLC-FLD methods reported in the literature for the quantification of steroids in clinical, pharmaceutical, and environmental applications, with a focus on the various approaches of fluorescent derivatization.
Article
Chemistry, Analytical
Maxime Meloche, Martin Jutras, Isabelle St-Jean, Simon de Denus, Gregoire Leclair
Summary: A sensitive LC-MS/MS assay was developed and validated for quantification of (S)-metoprolol and its metabolite in human plasma. The method showed stability and accuracy for clinical use, which could be further explored for investigating the clinical impact of (S)-MET concentrations.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Review
Chemistry, Analytical
Raghav Dogra, Mohit Kumar, Arvind Kumar, Marco Roverso, Sara Bogialli, Paolo Pastore, Uttam Kumar Mandal
Summary: Derivatization is a valuable practice for analyzing difficult analytes in complex matrices. The availability of derivatizing reagents has made analyte modification more feasible. Derivatization techniques have enhanced the analytical strength in recent years. Although conventional HPLC lacks sufficient sensitivity, combining it with derivatization can significantly improve sensitivity.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Qiao-Lian Duan, Hai-Wei Shi, Li Tan, Zhen Liu, Qing Huang, Wei Shen, Ling Cao, Hian Kee Lee, Sheng Tang
Summary: A new ultrahigh-performance supercritical fluid chromatography (UHPSFC) method was developed to solve the difficult separation problem of various biogenic amines (BAs) in gentamicin. The method achieved complete separation of 10 BAs using dansyl chloride derivatization and UHPSFC system. It demonstrated high sensitivity and accuracy, and met the quantification limits required for impurity determination.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Hui Liu, Qi Zhang, Hesong Huang, Mingliang Qiu, Lvjiang Hu, Xiaojuan Zhao, Chuanxi Xu, Zhiqiang Wang, Chenlei Zhang, Yang Zhan, Yongbing Sun
Summary: A simple and efficient HPLC-MS/MS method for the determination of 3-bromopyruvate (3-BrPA) in rat plasma has been developed for the first time. The method was fully validated and showed excellent linearity, accuracy, and extraction recovery. It was successfully applied in a pharmacokinetic study.
BIOMEDICAL CHROMATOGRAPHY
(2022)
Article
Biochemistry & Molecular Biology
Corey W. W. Manwaring, Jake A. A. Cravino, Margi Patel, Jonathan G. H. Stathakis, Arianne Soliven, Thirada Suktham, Ross Andrew Shalliker
Summary: Coffee is a popular beverage, with a growing market worth over USD 4 billion. Coffee fraud is a concerning issue, with up to one in five coffees being contaminated with cheaper blends. Climate change and the recent Brazil frost have further impacted coffee production, leading to a potential increase in coffee fraud. In this study, a novel approach using postcolumn derivatization is proposed to authenticate coffee samples in real-time, along with three mathematical similarity metrics.
Article
Biochemistry & Molecular Biology
Maria S. Synaridou, Vasilis Tsamis, Georgia Sidiropoulou, Constantinos K. Zacharis, Irene Panderi, Catherine K. Markopoulou
Summary: Amino acids have ergogenic effects on young athletes by enhancing, protecting, and restoring their muscular system. Encapsulating five relevant amino acids in chocolate pellets enhances daily intake among them. A reliable HPLC fluorimetric method was developed to detect and quantitatively determine amino acids in chocolate, with experimental design methodology used to optimize the clean-up procedure.
Article
Food Science & Technology
Nor Shifa Shuib, Bahruddin Saad
Summary: A new sample pretreatment technique, ISD mu SPE, coupled with high performance liquid chromatography, was used for simultaneous determination of aflatoxins in milk. Online photo-chemical derivatization and optimization of parameters led to high sensitivity and accuracy in the analysis.
Review
Chemistry, Analytical
Apostolia Tsiasioti, Paraskevas D. Tzanavaras
Summary: Glutathione, as the most abundant antioxidant in living organisms, plays a crucial role in cells due to its free thiol group. Its determination using liquid chromatography has been widely studied and proves significant in understanding the development of human diseases.
MICROCHEMICAL JOURNAL
(2023)
Article
Biochemical Research Methods
Burhan Ceylan, Evrim Kepekci Tekkeli, Cem Onal
Summary: A new HPLC method with fluorometric detection was developed for the determination of mesalazine in human plasma, and used to analyze pharmacokinetic parameters of the drug. The method was validated according to ICH criteria and showed good specificity, linearity, precision, accuracy, and robustness. The method can be used for bioequivalence and bioavailability investigations.
JOURNAL OF FLUORESCENCE
(2022)
Article
Biochemistry & Molecular Biology
Marzena Pawul-Gruba, Tomasz Kiljanek, Anna Madejska, Jacek Osek
Summary: A method for determining eight biogenic amines in ripened cheeses was developed and validated using HPLC-DAD, showing high accuracy and precision.
Article
Biochemistry & Molecular Biology
Yun Ai, Yan Ni Sun, Li Liu, Fang Yuan Yao, Yan Zhang, Feng Yi Guo, Wen Jie Zhao, Jian Li Liu, Ning Zhang
Summary: This study successfully characterized biogenic amines in different parts of Lycium barbarum L. using HPLC, revealing four different types of amines. The bark had the highest histamine content, while the flower had the highest putrescine content. There were significant differences in biogenic amine content among different parts of Lycium barbarum L., which should be considered when further utilizing them.
Article
Biochemistry & Molecular Biology
Marianna Ntorkou, Eleni Tsanaktsidou, Konstantina Chachlioutaki, Dimitrios G. Fatouros, Catherine K. Markopoulou
Summary: Homotaurine shows promising potential as a drug for treating neurodegenerative diseases such as Alzheimer's. The study investigates the permeability of homotaurine in vitro and finds that it exhibits superior permeability through the simulated blood-brain barrier compared to memantine, suggesting potential for enhanced drug delivery strategies targeting neurological conditions.
Article
Biochemical Research Methods
Fathalla Belal, Mokhtar Mabrouk, Sherin Hammad, Aya Barseem, Hytham Ahmed
Summary: A novel ecofriendly optical nanosensor for detecting Eplerenone (EPL) in biological samples was reported in this study. Highly luminescent N, S-CQDs were successfully prepared and used as a fluorescent probe for sensitive determination of EPL. The method showed promising results in drug formulations and spiked human serum samples.
JOURNAL OF FLUORESCENCE
(2021)
Article
Chemistry, Analytical
Sona Barghash, Heba Elmansi, Sawsan Abd El-Razeq, Fathalla Belal
Summary: A new smart spectrofluorometric method was developed for the simultaneous quantitation of amoxicillin and ethopabate. The method is based on measuring their first derivative synchronous amplitudes and has linear ranges and high sensitivity. It is applicable to various sample analyses with good percentage recoveries.
Article
Chemistry, Analytical
Abdallah M. Zeid, Jenny Jeehan M. Nasr, Fathalla Belal, Mohamed Walash, Noritada Kaji, Yoshinobu Baba
Summary: A green chiral lab-on-a-chip cyclodextrin-based microchip electrophoretic method was developed for enantioseparation of BCN and Phe enantiomers. The method utilized the synergistic effects of cyclodextrin and methylcellulose, achieving efficient separation in short time.
MICROCHEMICAL JOURNAL
(2021)
Article
Spectroscopy
M. M. Salim, Mona E. El Sharkasy, F. Belal, M. Walash
Summary: This study investigated the binding interaction between the antidepressant drug fluvoxamine and human serum albumin under simulated physiological conditions. The results showed that hydrophobic forces were the principal intermolecular forces stabilizing the complex, leading to a slight change in human serum albumin conformation.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Biochemical Research Methods
Tarek Elawady, Alaa Khedr, Nahed El-Enany, Fathalla Belal
Summary: Erdafitinib is a recently approved FGFR inhibitor for patients with metastatic bladder cancer. A validated HPLC-UV method was developed for the determination of erdafitinib in mouse plasma. The method was successfully applied to pharmacokinetic studies of the drug.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Spectroscopy
Abdallah M. Zeid, Jenny Jeehan M. Nasr, Fathalla Belal, Mohamed I. Walash, Yoshinobu Baba, Noritada Kaji
Summary: A lab-on-a-chip electrophoretic method coupled with LED-induced fluorescence detection was developed for the assay of three antiepileptic drugs in pharmaceutical formulations. The method involves derivatizing analytes with FITC offline, optimizing separation conditions, and using specific compounds to enhance separation efficiency. The sensitivity of the method was greatly enhanced through stacking, allowing for successful validation in pharmaceutical formulations.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Spectroscopy
Galal Magdy, Fathalla F. Belal, Ahmed M. Abdel-Megied, Ahmed F. Abdel Hakiem
Summary: Two simple synchronous spectrofluorimetric methods were developed for simultaneous determination of lesinurad and febuxostat with good sensitivity and accuracy in spiked human plasma.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Pharmacology & Pharmacy
H. A. Elmekawy, F. Belal, A. E. Abdelaziz, K. S. Abdelkawy, A. A. Ali, F. Elbarbry
Summary: In healthy volunteers, concurrent administration of atorvastatin with fixed-dose combination of sofosbuvir/ledipasvir led to increased AUC(0-infinity) of both atorvastatin and sofosbuvir. Close monitoring for possible side effects should be employed in clinical practice after co-administration of atorvastatin and FDCSL.
EUROPEAN JOURNAL OF CLINICAL PHARMACOLOGY
(2021)
Article
Chemistry, Applied
Galal Magdy, Ahmed F. Abdel Hakiem, Fathalla Belal, Ahmed M. Abdel-Megied
Summary: A simple green hydrothermal method was proposed for synthesizing highly fluorescent nitrogen and sulfur co-doped carbon quantum dots (N,S-CQDs), which were utilized as fluorescent nanosensors for sensitive spectrofluorimetric determination of salinomycin and maduramicin. The developed method showed high recoveries in estimating analytes in six raw matrices.
Article
Multidisciplinary Sciences
M. M. Tolba, F. Belal, M. M. Salim
Summary: A green synchronous spectrofluorimetric quantification method was developed for the simultaneous analysis of ETO, MOX, and NAL. The method showed good linear correlation and was successfully applied to pharmaceutical dosage forms and spiked human urine samples for estimation of the studied drugs.
ROYAL SOCIETY OPEN SCIENCE
(2021)
Article
Chemistry, Analytical
Heba Elmansi, Mohamed I. El-Awady, Sona Barghash, Sawsan Abd El-razeq, Fathalla Belal
Summary: A fast reliable micellar electrokinetic methodology was developed for the quantitation of six antimicrobial and anti-inflammatory drugs. The method showed rapidity, precision, and sensitivity, with a separation time of less than 7 minutes. The proposed method had a linear response range, detection limits, and quantitation limits suitable for analysis. The method's greenness was also evaluated and found to be excellent. Commercial preparations were investigated and statistically evaluated.
ACTA CHROMATOGRAPHICA
(2023)
Article
Chemistry, Analytical
Mohamed El-Awady, Sawsan Abdel-Razeq, Sona Barghash, Fathalla Belal
Summary: Efficient separation of five cardiovascular drugs has been achieved using a new micellar electrokinetic chromatographic method. The method is based on a general strategy that can be applied for the separation of different hydrophobic analytes, while maintaining high sweeping efficiency and sensitivity.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Heba Elmansi, Fathalla Belal, Mervat M. Hosny, Omar M. EL-Abassy
Summary: Sensitive and validated spectroscopic methods were developed for estimating hydrochlorothiazide and minoxidil through the reaction with NBD-CL. These methods were successfully applied for determining the drugs in dosage forms and human urine samples, proving to be economical, sensitive, and validated.
Article
Chemistry, Multidisciplinary
Sahar Zayed, Fatma Fouad, Fathalla Belal
Summary: The study successfully developed a new method to simultaneously determine six bronchodilators, which can save costs, solvents, and chemicals. It is a rapid, sensitive, and highly efficient analytical method.
JOURNAL OF THE IRANIAN CHEMICAL SOCIETY
(2021)
Article
Chemistry, Analytical
Magda M. Ayad, Mervat M. Hosny, Adel Ehab Ibrahim, Omar M. El-Abassy, Fathalla F. Belal
Summary: In recent years, the use of surfactants in reversed phase liquid chromatography has been steadily developing, with Brij-35 being studied as a less commonly used mobile phase additive. This method shows potential in making chromatographic methodologies greener and more eco-friendly by reducing the amount of organic solvents required for elution.
ACTA CHROMATOGRAPHICA
(2021)
