Article
Medical Laboratory Technology
Zheng Zhong, Yukai Huang, Qidang Huang, Shaoling Zheng, Zhixiang Huang, Weiming Deng, Tianwang Li
Summary: This study investigated the serum metabolic profiling of gout patients compared to healthy controls using UPLC-Q-TOF/MS, revealing significant differences in metabolites between the two groups. Bilirubin was identified as a potential biomarker for gout, and primary bile acid biosynthesis was suggested as a novel metabolic pathway.
CLINICA CHIMICA ACTA
(2021)
Article
Biochemistry & Molecular Biology
JuDong Yeo, Christopher C. Parrish
Summary: Shotgun lipidomics was used to identify and quantify phospholipids in salmon muscle tissue, focusing on omega-3 fatty acids distribution and eicosanoids. 43 PL species were identified, with 16:0-22:6 PtdCho being predominant. Additionally, prostaglandin E2 and prostaglandin F3 alpha were identified for the first time in salmon muscle.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agriculture, Multidisciplinary
Aipeng Hu, Fang Wei, Fenghong Huang, Ya Xie, Bangfu Wu, Xin Lv, Hong Chen
Summary: An ultrahigh-performance liquid chromatography coupled with mass spectrometry method was established for comprehensive lipidomic profiling of oilseeds, revealing significant variations in lipid composition and content among soybean, sesame, peanut, and rapeseed. The study provided a theoretical basis for the selection and breeding of oilseed varieties as well as processing of oilseed for the edible oil industry.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Medical Laboratory Technology
Zhi Yang, Xuhui Deng, Jinhua Zhu, Sujuan Chen, Chenze Jiao, Yucai Ruan
Summary: This study identifies six metabolic markers related to sphingolipid metabolism that have potential as diagnostic markers for stroke. The markers showed significant differential expression between stroke patients and healthy controls and demonstrated good diagnostic potential in distinguishing between the two groups.
CLINICA CHIMICA ACTA
(2024)
Article
Biochemical Research Methods
Shasha Qin, Jingzhuo Tian, Lianmei Wang, Yong Zhao, Dunfang Wang, Fang Wang, Jing Meng, Meiting Liu, Aihua Liang
Summary: This study developed a method for quantitatively analyzing 31 bile acids in different tissues of rats and applied it to drug-induced liver injury models. The results showed that bile acids exhibited different trends in different types of liver injury, making them more suitable for diagnosing cholestatic liver injury.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Applied
Jiayang Sun, Pengpeng Hu, Chunmao Lyu, Jinlong Tian, Xianjun Meng, Hui Tan, Wenxuan Dong
Summary: In this study, we used UPLC-QTOF-MS to dynamically monitor the lipid composition of hazelnut oil during storage. A total of 10 subclasses of 103 lipids were identified. After 24 days, the content of several lipids decreased significantly. We also identified 51 lipids that could be used as biomarkers to distinguish fresh and oxidized hazelnut oil.
Article
Medicine, Research & Experimental
Huan Li, Liang Wang, Jing Zhu, Jialing Xiao, Huining Yang, Huanyue Hai, Jiarui Hu, Lin Li, Yi Shi, Man Yu, Ping Shuai, Yuping Liu, Xueming Ju, Gang Wu, Yu Zhou, Bolin Deng, Bo Gong
Summary: The study aimed to find reliable serum biomarkers for diagnosing ankylosing spondylitis (AS). Serum samples from 20 AS patients and 20 healthy controls (HCs) were analyzed using UPLC-MS/MS. Differential metabolites between AS patients and HCs were identified, and pathway analysis and heat map were conducted. Predictive and diagnostic models were established using random forest (RF) and LASSO regression. A total of 61 differential metabolites were identified, and RF model showed 95% predictive accuracy and a high AUC. A diagnostic model consisting of nine metabolites was generated, capable of distinguishing HCs from AS with a high AUC. UPLC-MS/MS analysis proved to be a powerful tool for identifying AS metabolite profiles, and the identified biomarkers had diagnostic value for AS.
CLINICAL AND EXPERIMENTAL MEDICINE
(2023)
Article
Pharmacology & Pharmacy
Yin Yuan, Fei-Xue Dong, Xu Liu, Hong-Bin Xiao, Zhong-Guang Zhou
Summary: This study revealed that Magnoflorine has an intervention effect on prostatitis, inhibiting its development by regulating metabolic pathways and biomarkers. The results suggest that alkaloids could be an effective therapy for prostatitis, with specific metabolic pathways closely related to the condition identified.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Plant Sciences
Zhen Zhang, Shasha Yang, Xia Lin, You Huang, Xinyi Wei, Jinwei Zhou, Rui Li, Bin Deng, Chaomei Fu
Summary: The study investigated the essence of Spleen-Yang deficiency (SYD) through metabolomics and revealed the anti-SYD action mechanisms of Fuzi Lizhong pill (FLZP). Results showed that FLZP regulated biomarkers related to SYD, alleviating diarrhea and gastrointestinal motility disorder, and exhibiting anti-peroxidation, anti-inflammatory, and immune regulation effects.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Food Science & Technology
Lei Xia, Hifza Rasheed, Michael N. Routledge, Hang Wu, Yun Yun Gong
Summary: The study revealed high levels of mycotoxin exposure in the Pakistani population, with particular concern for aflatoxin B-1 and ochratoxin A. Further management is needed to address the health risks associated with exposure to these mycotoxins.
Article
Chemistry, Applied
Alba Macia, Maria-Paz Romero, Silvia Yuste, Iziar Ludwig, Anna Pedret, Rosa Maria Valls, Patricia Salamanca, Rosa Sola, Maria Jose Motilva, Laura Rubio
Summary: The study evaluated the metabolism and bioavailability of anthocyanins and other phenolics from red-fleshed apple and identified intake biomarkers. The results showed differences in intake biomarkers between the two apple varieties, which is of significance for understanding the relationship between these compounds and human health.
Article
Multidisciplinary Sciences
Lucia Pappalardo
Summary: Pomegranate is a valuable source of nutrition with medicinal benefits. As the demand for pomegranate fresh fruit juices increases, the issue of adulteration becomes a concern. This study utilized UV-visible spectroscopy and multivariate statistical analysis to detect adulteration of pomegranate juice with apple juice concentrate.
SCIENTIFIC REPORTS
(2022)
Article
Cardiac & Cardiovascular Systems
Jun Liu, Liangqiu Tang, Qiqi Lu, Yi Yu, Qiu-Gui Xu, Shanqiang Zhang, Yun-Xian Chen, Wen-Jie Dai, Ji-Cheng Li
Summary: This study identified potential plasma lipid biomarkers for early screening and prognosis of Acute Myocardial Infarction (AMI) using quantitative lipid technology and machine learning approach. The identified lipid metabolites showed good predictive ability in distinguishing AMI patients and had prominent performance in predicting re-admission of patients with AMI due to cardiovascular events.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Chemistry, Analytical
Xue-Lian Jiang, Yao Zhu, Gai-Fan Ma, Ping Liu, Lin-Lin Chen
Summary: The overall chemical profile of Renshen-Yangrong Pill (RYP), a traditional Chinese medicine, was investigated using UPLC-LTQ/Orbitrap/MS, and the quantitative distributions of representative components in the preparation were obtained. A total of 132 components were identified or characterized, with hesperidin and paeoniflorin being the most abundant constituents. This study provides valuable insights into the chemical composition of RYP and lays a foundation for further exploration of its effective components and quality control.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Food Science & Technology
Hong Xie, Yinping Li, Jiaxing Li, Yinglong Chen, Jing Li, Lixue Kuang, Syed Asim Shah Bacha, Tiejun Zhang, Yuehui Chao, Amin Mousavi Khaneghah
Summary: A novel method based on QuEChERS extraction and ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) detection was developed for analyzing 14 mycotoxins in peaches and peach products from China. The method accurately quantified mycotoxins with recoveries ranging from 84.6% to 117.6% and precision below 20%. Six mycotoxins were identified in diseased peaches, while fresh peaches showed no mycotoxin contamination. Peach products had contamination levels below quantitative limits and legal limits.
