Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 940, Issue -, Pages 59-65Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2013.09.017
Keywords
Mesoporous silica; Solid phase membrane tip extraction; Non-steroidal anti-inflammatory drugs; Urine
Funding
- Ministry of Higher Education Malaysia (MOHE) [QJ130000.2526.03H79]
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Mesoporous silica material, MCM-41, was utilized for the first time as an adsorbent in solid phase membrane tip extraction (SPMTE) of non-steroidal anti-inflammatory drugs (NSAIDs) in urine prior to high performance liquid chromatography-ultraviolet (HPLC-UV) analysis. The prepared MCM-41 material was enclosed in a polypropylene membrane tip and used as an adsorbent in SPMTE. Four NSAIDs namely ketoprofen, diclofenac, mefenamic acid and naproxen were selected as model analytes. Several important parameters, such as conditioning solvent, sample pH, salting-out effect, sample volume, extraction time, desorption solvent and desorption time were optimized. Under the optimum extraction conditions, the MCM-41-SPMTE method showed good linearity in the range of 0.01-10 mu g/mL with excellent correlation coefficients (r=0.9977-0.9995), acceptable RSDs (0.4-9.4%, n = 3), good limits of detection (5.7-10.6 mu g/L) and relative recoveries (81.4-108.1%). The developed method showed a good tolerance to biological sample matrices. (C) 2013 Elsevier B.V. All rights reserved.
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