Article
Food Science & Technology
Jing Pu, Bingye Zhao, Xin Liu, Shugang Li, Beibei Wang, Di Wu, Jinqiu Wang, Fang Geng
Summary: This study comprehensively compared the protein profiles and properties of chicken egg white and its three components. The proteomes of thick and thin egg white are similar, but thick egg white has higher abundance of ovomucin subunits and lysozymes. The properties of thick and thin egg white are significantly different. Additionally, the chalaza component has higher abundance of insoluble proteins and lower abundance of soluble proteins, which explains its insolubility. These findings are important for further research and development of egg white, such as thinning of egg white, understanding changes in egg white properties, and differential application of thick and thin egg white.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Chemistry, Analytical
Francis Berthias, Dale A. Cooper-Shepherd, Frederik H. V. Holck, James I. Langridge, Ole N. Jensen
Summary: The combination of cyclic ion mobility spectrometry (cIM) and electron capture dissociation (ECD) allows for the complete separation and sequencing of large isomeric peptides, providing a new method for identifying biologically important proteoforms in complex mixtures.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Runmin Yang, Jingjing Ma, Shu Zhang, Yu Zheng, Lusheng Wang, Daming Zhu
Summary: This study introduces a new storage format called mzMD for MS datasets and presents an algorithm to query this format for summarizing a given data window. Experimental results demonstrate the high speed and stability of mzMD in retrieving high-quality data window summaries.
Article
Biochemistry & Molecular Biology
Gabor Kecskemeti, Edit Toth-Molnar, Tamas Janaky, Zoltan Szabo
Summary: Tear samples are valuable sources of information for precision medicine, and this study evaluates a novel sample collection technique for human tear samples. The results show that this new technique is equally or even more suitable for proteomic sample collection compared to traditional methods.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Hirakjyoti Kakati, Aparup Patra, Bhargab Kalita, Abhishek Chanda, Srikanth Rapole, Ashis K. Mukherjee
Summary: This study used two proteomic approaches to analyze the composition of Indian monocled cobra venom. The venom was found to contain toxins from multiple families, with phospholipase A2 and three-finger toxins being the most abundant enzymatic and non-enzymatic proteins, respectively. Commercial antivenom showed poor efficacy in recognizing low molecular weight toxins, but specific antibodies for this venom were found in the antivenom.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Heba Badr, Ron Blutrich, Kaitlin Chan, Jiefei Tong, Paul Taylor, Wen Zhang, Ran Kafri, Hannes L. Rost, Ming-Sound Tsao, Michael F. Moran
Summary: Proteome analysis revealed co-expressed upregulated metabolism proteins highly conserved between primary NSCLC and patient-derived xenograft tumors. The encoded genes of these proteins are associated with the survival of NSCLC patients, suggesting their role in driving NSCLC through metabolic processes.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
Article
Immunology
Haoran Guo, Qi Wang, Tao Li, Jingwen Chen, Chao Zhang, Ying Xu, Qing Chang, Hangyi Li, Weiqiang Sun, Ruidi Han, Chi Wang, Chengbin Wang
Summary: This study discovered that ascending to high altitude can lead to physiological and molecular changes, resulting in some individuals being unable to acclimatize. Predicting acute mountain sickness (AMS) prior to ascent remains a challenge. Proteomic analysis revealed significant differences in inflammatory and immune-related processes between AMS-susceptible and AMS-resistant groups. Potential biomarkers, such as SAP, AAT, LTF, and HSP90-alpha, were identified for the prediction of AMS at low altitudes.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Food Science & Technology
A. Cimbalo, M. Frangiamone, G. Font, L. Manyes
Summary: This review highlights recent advances in using transcriptomics and proteomics as key tools to understand the molecular toxicity of mycotoxins. The most studied mycotoxin using transcriptomics is deoxynivalenol (DON), followed by aflatoxins (AFs) and zearalenone (ZEA). In contrast, proteomics mostly focuses on AFs, with some research on ZEA and DON. However, fewer studies have investigated the toxicological effects of emerging mycotoxins such as patulin, ochratoxin A, T-2 toxin, alternariol, and amino-14,16-dimethyloctadecan-3-ol. Transcriptomics commonly studies changes in gene expression related to the immune system, while proteomics focuses on cellular processes. The most commonly used techniques are RT-qPCR for gene expression analysis and liquid chromatography coupled with mass spectrometry for proteomics assays. The data gathered indicate an increased interest in using these omic approaches in the past five years. However, in vitro models are prioritized over in vivo and ex vivo models. Therefore, there is a need to enhance the use of in vivo models and alternative methods to better understand the mode of action of mycotoxins on animal and human health.
FOOD AND CHEMICAL TOXICOLOGY
(2022)
Article
Chemistry, Analytical
Yueting Xiong, Jun Yao, Yongtao Zheng, Fenglin Shen, Huanhuan Zhao, Jia Hu, Bing Leng, Pengyuan Yang, Xiaohui Liu
Summary: Protein biomarkers for intracranial aneurysm (IA) are crucial for early detection and diagnosis, and a comprehensive strategy involving tissue and serum analysis identified candidate biomarkers and established two diagnostic classifiers with high accuracy for IA and its rupture. The results suggest a valuable approach for biomarker discovery and patient stratification in IA.
Article
Plant Sciences
Jiankun Song, Jingsi Jiang, Le Kuai, Yue Luo, Meng Xing, Ying Luo, Yi Ru, Xiaoying Sun, Huiping Zhang, Taiyi Liu, Xin Li, Bin Li
Summary: Jueyin granules (JYG) may induce autophagy by up-regulating ApoA1 and inhibit the infiltration of CD4+ T cells and macrophages, thereby alleviating psoriatic inflammation.
Article
Immunology
Jia Huang, Jiachi Yu, Jianan Wang, Jiayu Liu, Wei Xie, Ruibing Li, Chengbin Wang
Summary: This study identified three novel candidate proteins as promising circulating biomarkers for clinical diagnosis and disease progression in ALD. The combination of these biomarkers showed superior efficacy in monitoring disease progression compared to existing liver fibrosis evaluation indices.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemical Research Methods
Utpal Bose, James A. Broadbent, Angela Juhasz, Shaymaviswanathan Karnaneedi, Elecia B. Johnston, Sally Stockwell, Keren Byrne, Vachiranee Limviphuvadh, Sebastian Maurer-Stroh, Andreas L. Lopata, Michelle L. Colgrave
Summary: We explored the influence of protein extraction buffer selection and processing method on protein yield and allergen detection in processed and unprocessed black soldier fly (BSF) samples. We detected a total of 33 putative allergens by comparing the detected BSF proteins with sequences from public allergen protein databases. A targeted LC-MRM-MS assay was developed for tropomyosin, highlighting the importance of buffer selection and processing conditions in reducing allergenicity of BSF samples.
JOURNAL OF PROTEOMICS
(2022)
Review
Biochemistry & Molecular Biology
Rekha Raghunathan, Kathleen Turajane, Li Chin Wong
Summary: Neurodegenerative diseases such as ALS and PD lack biomarkers for diagnosis and treatment efficacy. Proteomic studies have identified potential biomarker proteins in ALS and PD and explored disease-related post-translational modifications. Comparative analysis of relative and absolute quantitative proteomic approaches and recent technological advancements may help identify suitable biomarkers for early diagnosis and treatment efficacy of these diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Elisa Ceccherini, Giovanni Signore, Lorena Tedeschi, Federico Vozzi, Nicoletta Di Giorgi, Elena Michelucci, Antonella Cecchettini, Silvia Rocchiccioli
Summary: In this study, a water-stable and freely dispersible nanoformulation of curcumin was prepared and characterized. The effects of this nanoformulation on cholangiocytes under fibrotic pathological conditions were investigated. The results showed that curcumin nanoformulation exhibited anti-inflammatory properties and induced programmed cell death to counteract cellular damages induced by TGF-beta. It also positively influenced the expression of proteins involved in TGF-beta-mediated fibrosis. These findings provide insights into the mechanisms associated with cholangiopathies and potential for targeted therapies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Kanokwan Nonthawong, Chantragan Srisomsap, Daranee Chokchaichamnankit, Jisnuson Svasti, Pharima Phiriyangkul
Summary: This study compared the proteins in fresh and powdered fish from skipjack tuna and Nile tilapia using proteomic approach. It identified potential allergens and discovered peptide markers for fish adulteration. Interestingly, the potential allergens in fish were similar to peptides from chicken powder and egg yolk powder.
Article
Biochemical Research Methods
Vincent Pepermans, Michael T. Rerick, Bart Degreef, Sebastiaan Eeltink, Stephen G. Weber, Gert Desmet
Summary: This study reports on the design and performance of in-house built column cartridges that can be directly screwed into the ports of a commercial rotor-stator valve to minimize extra-column band broadening and pressure-drop losses during ultra-fast separations. Results showed that the separation efficiency of the in-house fabricated cartridge columns was low, possibly due to the inner diameter of the column cartridges falling into a region most likely to suffer from packing problems.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Review
Chemistry, Analytical
Thomas Themelis, Ali Amini, Jelle De Vos, Sebastiaan Eeltink
Summary: In spatial comprehensive three-dimensional chromatography (3D-LC), unprecedented resolving power is achieved within a three-dimensional separation space, with the maximum peak capacity being the product of peak capacities achieved in individual dimensions. The parallel development of second- and third-dimension separation stages overcomes the limitations of conventional multi-dimensional approaches.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biochemical Research Methods
Sam Wouters, Sebastiaan Eeltink, Rob Haselberg, Govert W. Somsen, Andrea F. G. Gargano
Summary: A membrane-based microfluidic chip was developed for selective post-column removal of TFA anions from HILIC, improving ionization and reducing TFA adduct formation. The device's multichannel design allows the use of 0.1% TFA mobile phases while maintaining separation selectivity and efficiency.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Editorial Material
Biochemical Research Methods
Jelle De Vos, Sebastiaan Eeltink
Article
Biochemical Research Methods
Raphael Ewonde, Jelle De Vos, Ken Broeckhoven, Daniel Esser, Sebastiaan Eeltink
Summary: This study assessed the separation performance of hydrophobic interaction chromatography for intact protein analysis using non-porous butyl polymethacrylate phases. It was found that the maximum peak capacity varied under different gradient volumes and flow rates, with a coupled column system improving gradient performance and decreasing particle size leading to higher peak capacities.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Medicine, General & Internal
Thomas Gentinetta, John D. Belcher, Valerie Bruegger-Verdon, Jacqueline Adam, Tanja Ruthsatz, Joseph Bain, Daniel Schu, Lisa Ventrici, Monika Edler, Hadi Lioe, Kalpeshkumar Patel, Chunsheng Chen, Julia Nguyen, Fuad Abdulla, Ping Zhang, Andreas Wassmer, Meena Jain, Marcel Mischnik, Matthias Pelzing, Kirstee Martin, Roslyn Davis, Svetlana Didichenko, Alexander Schaub, Nathan Brinkman, Eva Herzog, Adrian Zuercher, Gregory M. Vercellotti, Gregory J. Kato, Gerald Hoebarth
Summary: People living with sickle cell disease (SCD) often suffer from acute pain due to blood vessel blockage. This study discovered that hemopexin, a protein found in plasma, can alleviate the symptoms of vaso-occlusion by inhibiting inflammatory reactions and reducing the expression of adhesion proteins. Animal experiments and clinical trials have shown promising results, suggesting that hemopexin could be a potential treatment for SCD.
JOURNAL OF CLINICAL MEDICINE
(2022)
Review
Chemistry, Analytical
Stan Perchepied, Harald Ritchie, Gert Desmet, Sebastiaan Eeltink
Summary: Having a good understanding of the properties of stationary phases and the driving forces during the column packing process is essential for developing efficient packed-bed column technologies for separation science. This article discusses the properties of commonly-used silica particulate materials as stationary phases, including fully-porous and core-shell silica particles, and provides an overview of commonly used column hardware and frit technologies. Different packing approaches, such as dry packing, high-pressure slurry packing, electrokinetic packing, and packing using centrifugal forces, are also discussed, with a focus on the sedimentation of particles in slurries and the interaction between particles during the packing process and how they affect the resulting sphere packing.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Thomas Themelis, Jelle De Vos, Sebastiaan Eeltink
Summary: This article discusses the design aspects of microfluidic chips for spatial three-dimensional chromatography and establishes the corresponding kinetic performance limits using a Pareto-optimality approach. The advantages and disadvantages of integrating different separation mechanisms are discussed, and novel equations are proposed to determine the peak capacity and account for sample modulation. A microfluidic spatial prototype chip has been created with the potential to achieve a high peak capacity within a short analysis time.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Daniel Meston, Thomas Themelis, Zhuoheng Zhou, Jelle De Vos, Mauro De Pra, Frank Steiner, Ilse Becue, Els Daeseleire, Gert Desmet, Sebastiaan Eeltink
Summary: This study discusses the development of a UHPLC method that allows for ultra-high-resolution separations in gradient mode for analyzing complex residual multi-class antibiotic samples in food products. The study shows that high pressure operation with small particle size columns can achieve high resolving power, with the gradient steepness playing a dominant role in peak capacity generation.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Analytical
Thomas Collet, Benny Wouters, Sebastiaan Eeltink, Philipp Schmidt, Kristof Ramharter, Annick Hubin
Summary: This study investigates the time dependent characteristics of thiourea in copper plating process using ORP-EIS and ion chromatography to analyze the electrochemical behavior of the solution and the concentration of thiourea.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2022)
Article
Medicine, Research & Experimental
Saw Yen Ow, Eugene A. A. Kapp, Vesna Tomasetig, Anton Zalewski, Jason Simmonds, Con Panousis, Michael J. J. Wilson, Andrew D. D. Nash, Matthias Pelzing
Summary: Hageman factor (FXII) is vital in blood clotting and a target for treating hereditary angioedema (HAE). CSL312, a high-affinity human antibody, successfully inhibits activated FXII activity and is being tested in Phase 3 clinical trials for HAE. Structural studies showed that the antibody interacts with specific regions in FXII, suggesting potential binding sites for garadacimab on the beta chain of activated FXII (beta-FXIIa).
Article
Biochemical Research Methods
Ali Amini, Rosanne M. Guijt, Thomas Themelis, Jelle De Vos, Sebastiaan Eeltink
Summary: DLP 3D printing is an efficient additive manufacturing method for fabricating analytical microdevices. It utilizes a digital micromirror device to direct light and polymerize a liquid resin layer by layer. Advances in vat and lift design, projector technology, and resin composition enable the accurate fabrication of microchannel structures as small as 18 x 20 μm. This review presents the latest advances in DLP 3D printing technology, including instrument set-up and resin formulation, and highlights key efforts in fabricating microdevices for emerging (bio-)analytical chemistry applications such as colorimetric assays, extraction, and separation.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Multidisciplinary
Ravi Sharma, Zhuoheng Zhou, Thomas Themelis, Tom R. C. Van Assche, Sebastiaan Eeltink, Joeri F. M. Denayer
Summary: In this study, the low trace-level removal of perfluorooctanesulfonic acid (PFOS) from aqueous solutions using zeolitic imidazolate framework-8 (ZIF-8)-coated copper sheet (ZIF-8@Cu) composite is reported for the first time. The composite showed the highest removal rate of 98% and remained consistent over a wide range of concentrations. The morphological and structural characterization revealed surface degradation of ZIF-8 crystals, but despite this, ZIF-8 effectively removes PFOS molecules from aqueous solutions.
Article
Chemistry, Analytical
Koudi Zhu, Matthias Pursch, Bram Huygens, Sebastiaan Eeltink, Gert Desmet
Summary: The possibility of reducing pre-column band broadening (PreCBB) by using a sandwiching method with an immiscible liquid was investigated. It was found that the method can significantly improve the efficiency of early-eluting compounds. The presence of the sandwich plugs does not affect the repeatability of the analysis or the linearity of the calibration curves. However, there is a slight adverse effect on late-eluting peaks under certain conditions.
ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Stan Perchepied, Zhuoheng Zhou, Goran Mitulovic, Sebastiaan Eeltink
Summary: Ion mobility spectrometry-mass spectrometry (IMS-MS) has experienced rapid growth in proteomic studies, offering improved dynamic range, throughput, quantitation precision, and proteome coverage. This review provides an introduction to IMS operation modes, discusses the advantages and limitations, and explores the principles of trapped IMS-MS (TIMS-MS). The emerging applications of TIMS focusing on sample throughput and sensitivity, as well as the potential for intact protein analysis, are also reviewed.
JOURNAL OF SEPARATION SCIENCE
(2023)