Article
Crystallography
Shenghui Zhong, Peng Liu, Jinsong Ding, Wenhu Zhou
Summary: The MTX-PEI@HA nanoparticles prepared in this study showed selective internalization via CD44 receptor-mediated endocytosis in activated macrophages, effectively mitigating inflammatory arthritis with notable safety at a high dose of MTX in in vivo mice model study. The distinct advantages of aqueous-synthesized NPs coated with HA for arthritis-selective targeted delivery make MTX-PEI@HA NPs a promising MTX-based nanoplatform for the treatment of RA.
Article
Pharmacology & Pharmacy
Ana Socorro Rodriguez-Baez, Ana Patricia Huerta-Garcia, Susanna Edith Medellin-Garibay, Cristian Jazmin Rodriguez-Pinal, Marco Ulises Martinez-Martinez, David Herrera-Van Oostdam, Carlos Abud-Mendoza, Silvia Romano-Moreno, Rosa del Carmen Milan-Segovia
Summary: The safety and efficacy of low-dose methotrexate administered daily or weekly in rheumatoid arthritis patients were investigated in this study. The results showed a good response to methotrexate treatment in both daily and weekly dosage regimens, with similar adherence patterns. Additionally, methotrexate polyglutamate 3 concentrations could serve as a useful tool for therapeutic drug monitoring.
BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY
(2022)
Article
Rheumatology
Cecile Gaujoux-Viala, Christophe Hudry, Elena Zinovieva, Helene Herman-Demars, Rene-Marc Flipo
Summary: The STRATEGE study aimed to describe treatment strategies in RA biologic DMARD-naive patients with an inadequate response to MTX therapy, and to compare clinical efficacy of different therapeutic strategies after 6 months. The study found that optimizing MTX treatment before initiating biological treatment is crucial and highlights the importance of a treat-to-target strategy.
Article
Biochemical Research Methods
Duck Hyun Kim, Yeong Suk Yoo, Hee Jin Yoo, Yoon Ji Choi, Soon Ae Kim, Dong-Hyuk Sheen, Sang Kwang Lee, Mi Kyoung Lim, Kun Cho
Summary: The study developed a new LC-ESI-MS/MS method for accurately measuring MTX-Glu in hair cells, red blood cells, and serum. This method was both sensitive and precise within the clinically relevant range.
JOURNAL OF MASS SPECTROMETRY
(2021)
Article
Pharmacology & Pharmacy
Hongxing Wang, Hui Wu, Kehua Fang, Xiaotian Chang
Summary: The study showed significantly increased levels of uridine diphosphate (UDP) in the synovial fluid of rheumatoid arthritis (RA) patients, positively correlated with disease markers. UDP was found to promote RA pathogenesis by stimulating P2Y6 activity to increase IL-6 production.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Rheumatology
Paul Studenic, Daniel Aletaha, Maarten de Wit, Tanja A. Stamm, Farideh Alasti, Diane Lacaille, Josef S. Smolen, David T. Felson
Summary: This study aimed to externally validate a revised Boolean remission criteria using a higher patient global assessment (PtGA) threshold and validate the provisionally endorsed index-based criteria. The results showed that using the higher PtGA threshold increased the proportion of patients classified as achieving remission under the "Boolean2.0" criteria, improved agreement with index-based criteria, and did not compromise predictive value for radiographic or functional outcomes. The study findings were endorsed by ACR and EULAR.
ANNALS OF THE RHEUMATIC DISEASES
(2023)
Article
Biology
Sidrah Jahangir, Peter John, Attya Bhatti, Muhammad Muaaz Aslam, Javaid Mehmood Malik, James R. Anderson, Mandy J. Peffers
Summary: This study aimed to identify potential serum biomarkers for rheumatoid arthritis in the Pakistani population. A total of 10 differentially expressed proteins were identified and pathway analysis predicted macrophage-related signaling pathways.
Article
Biochemical Research Methods
Dala N. Daraghmeh, Mahin Moghaddami, Larisa Bobrovskaya, Susanna M. Proudman, Michael D. Wiese
Summary: This study presents a rapid and highly sensitive method to measure MTX-PGs metabolite concentrations, which has been validated in various matrices and improves greenness.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Plant Sciences
Kishor Mazumder, Yasmeen Nazim Ruma, Rasheda Akter, Asma Aktar, Mir Monir Hossain, Zinnat Shahina, Santosh Mazumdar, Philip G. Kerr
Summary: The study demonstrated that secondary metabolites produced by endophytic fungi in Elaeocarpus floribundus Blume have the potential to alleviate pain and inflammation associated with rheumatoid arthritis, providing scientific evidence for the traditional use of the plant.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Chang Shu, Teng-Fei Li, Duo Li, Zhong-Qiu Li, Xing-Hua Xia
Summary: Pomalidomide, an IMiD approved by the FDA for multiple myeloma treatment, was quantified in human plasma using a validated LC-MS/MS method. The method was successfully applied to clinical pharmacokinetic studies with positive results.
CHINESE CHEMICAL LETTERS
(2022)
Article
Rheumatology
Eva Vermeer, Renske C. F. Hebing, Maartje M. van de Meeberg, Marry Lin, Tim G. J. de Meij, Eduard A. Struys, Gerrit Jansen, Michael T. Nurmohamed, Maja Bulatovic Calasan, Robert de Jonge
Summary: There is no conclusive evidence supporting the superiority of either oral or subcutaneous administration of methotrexate (MTX) in immune-mediated inflammatory disorders (IMIDs). Subcutaneous administration leads to higher plasma bioavailability and initial accumulation of MTX-polyglutamates (MTX-PGs) in red blood cells (RBCs), but the difference seems to disappear over time. The choice of administration route may be based on shared decision-making, offering patients the option of either oral or subcutaneous administration of MTX based on individual preferences and tolerability.
CURRENT RHEUMATOLOGY REPORTS
(2023)
Article
Chemistry, Analytical
Andisiwe Malo, Tracy Kellermann, Elisa H. Ignatius, Kelly E. Dooley, Rodney Dawson, Anton Joubert, Jennifer Norman, Sandra Castel, Lubbe Wiesner
Summary: A method for the extraction and quantification of pretomanid in human plasma using LC-MS/MS was developed and validated. The method showed good performance in accuracy, precision, selectivity, sensitivity, recovery and stability, and was specific for pretomanid.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Rheumatology
Renske C. F. Hebing, Marry Lin, Maja Bulatovic Calasan, Ittai B. Muller, Sohaila Mahmoud, Sandra Heil, Eduard A. Struys, Bart J. F. van den Bemt, Jos W. R. Twisk, Willem Lems, Michael T. Nurmohamed, Gerrit Jansen, Robert de Jonge
Summary: This study investigates the pharmacokinetics of methotrexate polyglutamate (MTX-PG) accumulation in red blood cells (RBCs) and peripheral blood mononuclear cells (PBMCs) in patients with early rheumatoid arthritis (RA) after oral and subcutaneous MTX treatment. The results show that there are disparate pharmacokinetic profiles between PBMCs and RBCs, with higher accumulation of MTX-PG in PBMCs. The distribution profile of MTX-PG in PBMCs remains constant over 6 months, while in RBCs, the main PG-moiety changes from MTX-PG(1) to MTX-PG(3).
ANNALS OF THE RHEUMATIC DISEASES
(2023)
Article
Microbiology
Xiaofei Wang, Xiaojuan Wang, Tongkai Cai, Yulin Qin, Ling Li, Yuanying Jiang, Bing Han, Yongbing Cao
Summary: In this study, a simple and sensitive liquid chromatography with tandem mass spectrometry (LC-MS/MS) method was developed to quantify the intracellular fluconazole (FLC) content in the opportunistic human fungal pathogen Candida albicans. The method was found to be accurate, efficient, and reproducible, and was successfully validated in 36 clinical isolated strains.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Rheumatology
Delila Singh, Nina Hesse, Alla Skapenko, Hendrik Schulze-Koops
Summary: Methotrexate is associated with rare bone lesions that are often misdiagnosed as osteoporotic fractures. Early diagnosis is crucial for treatment and prevention of further osteopathology. This case highlights the importance of raising awareness and taking appropriate therapeutic measures, including discontinuing methotrexate.