Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 903, Issue -, Pages 171-176Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2012.07.017
Keywords
Glucosinolate; Glucoraphanin; Sulforaphane; Broccoli, Cabbage; Kale; Brassica oleracea; Cole; LC-ESI-MS
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Sulforaphane is an isothiocyanate well known for its potential health benefits. With the aim of finding sulforaphane supply sources, its precursor, glucoraphanin, was widely searched for among Brassica oleracea varieties. Quantitative profiling of seven glucosinolates by LC-MS analysis was performed on 6 cultivars of broccoli, 32 of cabbage and 24 cultivars of kale. The glucoraphanin levels found in three cultivars of cabbage and six cultivars of kale were comparable with, or even higher than, the highest of broccoli (119.4 mg/100g FW). The most promising group belonged to the black kale, Cavolo nero. Use of a C30 column and an ammonium formate buffer in LC-MS and a micro plate solid phase extraction technique was highly effective. (C) 2012 Elsevier B.V. All rights reserved.
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