Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 895, Issue -, Pages 162-168Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2012.03.009
Keywords
Microdialysis sampling; Liquid chromatography-quadrupole time-of-flight mass spectrometry; Panax notoginseng; Ginsenosides; Bile; Metabolites
Funding
- NIH/NCCAM [P01 AT004418, K01 AT005362]
- National Science Foundation of China [81130068, 30973884]
- Program for Changjiang Scholars and Innovative Research Teams in Universities [IRT0868]
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A dynamic microdialysis sampling method with liquid chromatography-quadrupole time-of-flight mass spectrometry (Q-TOF-MS) was developed for rapid and sensitive analysis of the metabolite profile of Panax notoginseng extract (PNE) in rat bile. In vivo studies in male Sprague-Dawley rats were performed with microdialysis probes implanted into the bile duct before bile samples were collected from 0 to 12 h. Metabolites of PNE were identified using dynamic adjustment of the fragmentor voltage to produce structure-relevant fragment ions. The mass accuracy of precursor and fragment ions was typically within 5 ppm of the theoretical values. We identified 7 compounds: 4 parent compounds (notoginsenoside R1, ginsenosides Rg1, Rbl, and Rd) and 3 metabolites (ginsenosides Rg2, Rh2, and compound K). Data from this study suggest that this microdialysis technique could be used in notoginseng saponin metabolic animal studies. Published by Elsevier B.V.
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