Article
Biochemical Research Methods
Ryan Nguyen, Jennifer C. Goodell, Priya S. Shankarappa, Sara Zimmerman, Tyler Yin, Cody J. Peer, William D. Figg
Summary: Remdesivir has been approved by the FDA as the first antiviral drug to treat COVID-19, and a rapid and sensitive LC-MS/MS assay has been developed for its quantification in human plasma with high accuracy and precision.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Biochemical Research Methods
Michiel Vandenbosch, Sylvia P. Nauta, Anastasiya Svirkova, Martijn Poeze, Ron M. A. Heeren, Tiffany Porta Siegel, Eva Cuypers, Martina Marchetti-Deschmann
Summary: In this study, the sample preparation protocol for undecalcified bone samples was optimized for targeted and untargeted applications in forensic and preclinical research. The combination of gelatin and carboxymethyl cellulose (CMC) was found to best support the bone tissue during sectioning. Different cutting methods were successfully applied to rat, mouse, and human bone samples, demonstrating the versatility of the developed sectioning method. The detection of targeted substances (methadone and EDDP) and untargeted substances (unknown lipids) was achieved using specific matrix materials, showcasing the potential for future forensic and preclinical investigations.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Julian Lozano-Castellon, Anallely Lopez-Yerena, Alexandra Olmo-Cunillera, Olga Jauregui, Maria Perez, Rosa Ma Lamuela-Raventos, Anna Vallverdu-Queralt
Summary: Extra virgin olive oil (EVOO) is rich in nutritional and antioxidant compounds compared to other vegetable oils, making it an important component of the Mediterranean diet. This study developed a simple UHPLC-ESI-MS/MS method for quantifying secoiridoids in EVOO, overcoming challenges posed by their complex transformations during production and analysis. Validation of the method according to AOAC guidelines showed satisfactory results in terms of matrix effect and recoveries.
Article
Chemistry, Analytical
Juan L. Benede, Alberto Chisvert, Rafael Lucena, Soledad Cardenas
Summary: The study presents a proof-of-concept of using a polyamide-coated paper-based sorptive phase for simultaneous extraction of six different penicillin-derived antibiotics from human saliva samples. The method features simple synthesis, low cost, and high efficiency.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biochemical Research Methods
Max Sauter, Kathrin Foerster, Julia Benzel, Stefan Pfister, Kristian W. Pajtler, Walter E. Haefeli, Juergen Burhenne
Summary: An UPLC-MS/MS assay was established for the investigation of selinexor in mice, with high sensitivity and scalable sample processing capabilities. The assay showed high accuracy and was suitable for studying selinexor in small animal experiments.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Review
Chemistry, Analytical
Daniel Torregrosa, Guillermo Grindlay, Luis Gras, Juan Mora
Summary: ICP-MS is commonly used in life sciences for element content and speciation studies, but can also serve as a detector for immunoassays. Despite its advantages such as high sensitivity, multiplexed capabilities, and robustness, immunoassays based on ICP-MS detection are not well-known and lack significant breakthroughs in routine clinical applications.
MICROCHEMICAL JOURNAL
(2021)
Article
Biochemical Research Methods
Kiran Patil, Amin Pathan, Sukanta Naik, Vineet Zope, Rajesh Chavan, Ravindra Yeole
Summary: A precise and accurate LC-MS/MS bioanalytical method has been developed and validated for quantification of WCK 4234 and meropenem in dog plasma, allowing assessment of pharmacokinetics in beagle dogs.
BIOMEDICAL CHROMATOGRAPHY
(2022)
Article
Chemistry, Analytical
Andy Hsien Wei Koh, Russ Chess-Williams, Anna Elizabeth Lohning
Summary: This study evaluated the quantities of biogenic amines in pre-workout supplements containing bitter orange and compared them to standardized reference materials. The results showed that most supplements did not meet the ratios found in the reference materials, suggesting potential authenticity issues with the plant-derived active components in these supplements.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Chemistry, Analytical
Harvey E. Johnston, Kranthikumar Yadav, Joanna M. Kirkpatrick, George S. Biggs, David Oxley, Holger B. Kramer, Rahul S. Samant
Summary: Complete and reproducible extraction of protein is crucial for proteome analysis. Solvent precipitation SP3 (SP4) is proposed as an alternative method, which captures protein aggregates by centrifugation instead of magnetism. SP4 provides equivalent or higher protein yields and improved reproducibility. It also recovers more low-solubility and transmembrane proteins, offering higher recovery rates.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Shardrack O. Asare, Kimberly R. Dean, Bert C. Lynn
Summary: Lignin and lignans are natural products found in plant cell walls, with research historically focusing on lignin degradation techniques and the interesting biological activities of lignans. Studies have shown that both lignin and lignan dimers can possess multiple chiral centers, leading to observations of enhanced activity in one diastereomer of a pair. Tandem mass spectrometry and HPLC can be used to differentiate diastereomer pairs in these series.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Review
Biochemical Research Methods
Sven Heiles
Summary: Metabolomics and lipidomics are driving forces in the omics era, with tandem mass spectrometry methods playing a crucial role in characterizing the structural details of metabolites and lipids. Recent advancements in alternative tandem MS methodologies aim to provide full structural characterization of these compounds. The review critically examines the principles, capabilities, drawbacks, and initial applications of these advanced tandem mass spectrometry strategies in the fields of metabolomics and lipidomics.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Review
Spectroscopy
Xuelu Ding, Kun Liu, Zhenyan Shi
Summary: Laser desorption/ablation postionization (LD/API) techniques have been rapidly developing in the field of mass spectrometry, offering solutions to ionization of neutral substances and showing potential in breakthroughs in bioanalytical applications.
MASS SPECTROMETRY REVIEWS
(2021)
Article
Chemistry, Analytical
Bruno Charlier, Albino Coglianese, Francesca Felicia Operto, Federica De Rosa, Francesca Mensitieri, Giangennaro Coppola, Amelia Filippelli, Fabrizio Dal Piaz, Viviana Izzo
Summary: Therapeutic drug monitoring is crucial for improving the quality of antiepileptic drug use, especially in specific clinical situations. A novel method for measuring perampanel concentration in plasma samples was developed and compared with a commercially available method, showing the potential for clinical routine use as an alternative to LC-MS/MS.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Environmental Sciences
Laura Rohler, Pernilla Bohlin-Nizzetto, Pawel Rostkowski, Roland Kallenborn, Martin Schlabach
Summary: Long-term monitoring of regulated organic chemicals in ambient air provides valuable information about their environmental fate and trends, essential for evaluating regulatory effectiveness. New suspect and non-target screening analytical strategies are effective in identifying emerging compounds.
ATMOSPHERIC CHEMISTRY AND PHYSICS
(2021)
Article
Chemistry, Analytical
Robert S. Plumb, Thomas McDonald, Paul D. Rainville, Jason Hill, Lee A. Gethings, Kelly A. Johnson, Ian D. Wilson
Summary: Vacuum jacketed column (VJC) technology can significantly improve the performance of UHPLC systems, enhancing chromatographic separation and increasing detection capabilities for metabolites. Using the VJC system can reduce peak tailing, increase peak capacity, and enhance the intensity and response of MS peaks.
ANALYTICAL CHEMISTRY
(2021)