Article
Biochemistry & Molecular Biology
Berkley Luck, Thomas D. Horvath, Kristen A. Engevik, Wenly Ruan, Sigmund J. Haidacher, Kathleen M. Hoch, Numan Oezguen, Jennifer K. Spinler, Anthony M. Haag, James Versalovic, Melinda A. Engevik
Summary: The study revealed that Bifidobacterium dentium has the capacity to produce the important neurotransmitter gamma-aminobutyric acid (GABA) and can impact neurotransmitter concentrations in vivo, indicating a potential role of B. dentium in neurotransmitter regulation.
Article
Biochemical Research Methods
Roberta Rodrigues Viana, Ana Miguel Fonseca Pego, Tiago Franco de Oliveira, Eliane Dallegrave, Sarah Eller
Summary: This study developed a fast and simple analytical method for determining the concentrations of neurotransmitters in rat brain tissue. The method proved to be simple, rapid, and useful for estimating neurotransmitter concentrations and investigating the mechanism of action of psychoactive substances and neurological and psychiatric diseases.
BIOMEDICAL CHROMATOGRAPHY
(2022)
Article
Environmental Sciences
Inmaculada Fuertes, Carlos Barata
Summary: A study was conducted to develop an accurate analysis method for neurotransmitters in Daphnia magna. The results showed how neurotransmitter systems in D. magna are affected by chemical compounds, validating the importance of analyzing neurotransmitter levels in environmental neurotoxicity studies.
Article
Biochemistry & Molecular Biology
Yichen Wang, Renjin Zheng, Pingping Wu, Youjia Wu, Lingyi Huang, Liying Huang
Summary: This study investigated the efficacy of ethyl acetate fraction extract of Althaea rosea flower in treating TTX poisoning. Using LC-MS/MS, changes in the content of nine neurotransmitters in rat brain tissue were analyzed, and the results indicated that the ethyl acetate extract of Althaea rosea flower had a therapeutic effect on TTX poisoning.
Article
Chemistry, Multidisciplinary
Lulu Guo, Jian Mao, Qidong Zhang, Wu Fan, Dingzhong Wang, Zhonghao Li, Jiaqiang Huang, Jianping Xie
Summary: The metabolism and disposition of nicotine in the brain play a crucial role in determining its exposure. A new method was developed to analyze the dynamic profiles of nicotine metabolites and neurotransmitters. Inhibiting nicotine metabolism can enhance the residence of nicotine in the brain and its local neurotransmitter effects.
FRONTIERS IN CHEMISTRY
(2023)
Article
Biochemical Research Methods
Ala A. Alhusban, Alaa M. Hammad, Lujain F. Alzaghari, Aliaa Shallan, Khaldoun Shnewer
Summary: Tobacco smoking is a preventable cause of fatal diseases. This study aims to develop a fast and sensitive method for measuring neurotransmitter levels in rat brain after exposure to tobacco cigarettes. The results show that exposure to tobacco cigarettes significantly increases neurotransmitter levels, potentially activating the nicotine dependence pathway.
BIOMEDICAL CHROMATOGRAPHY
(2023)
Article
Biochemical Research Methods
Witold M. M. Winnik, William Padgett, Emily M. M. Pitzer, David W. W. Herr
Summary: This study applied label-free quantitation (LFQ) to analyze the proteome of rat brain cortex during early postnatal period. By comparing the datasets of published mouse and rat cortex proteomics, similarities and differences were identified. Importantly, significant similarities were found between rat cortex and mouse cortex, although mice had lower abundance of synaptic proteins.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Lukasz Chrobok, Sylwia Bajkacz, Jasmin Daniela Klich, Marian Henryk Lewandowski
Summary: Orexins are neuropeptides synthesised mainly in the brain lateral hypothalamic area, playing a vital role in feeding behaviour, regulation of the sleep-wake cycle and circadian rhythms. This study developed a LC-MS/MS method for quantitative bioanalysis of orexin in the rat vitreous body, showing potential implications on circadian regulation of vision with the first demonstration of daily rhythm of orexin synthesis by the retina.
Article
Biochemical Research Methods
Lei Gao, Zijia Zhang, Zijin Feng, Wenlong Wei, Wenyong Wu, Haijuan Zhi, Yanping Deng, Huali Long, Min Lei, Jinjun Hou, De-an Guo, Wanying Wu
Summary: This study established a sensitive, reproducible, and rapid liquid chromatography method with tandem mass spectrometry detection for simultaneous quantitative analysis of 16 neurotransmitters and their metabolites in rats. The intervention with Uncaria, a traditional Chinese medicine, showed significant effects on regulating the levels of neurotransmitters in hypertensive rats, indicating its potential in treating symptoms related to the nervous system. The results suggest a potential relationship between changes in plasma neurotransmitters and hypertension pathogenesis, providing valuable insights into the mechanisms of Uncaria in lowering blood pressure.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Food Science & Technology
Xuan Zeng, Yuying Zheng, Yan He, Wei Peng, Weiwei Su
Summary: Naringin is a dietary flavonoid glycoside with multiple bioactivities. This study developed a rapid and sensitive method for simultaneous determination of ten flavonoid metabolites of naringin in rat urine. The results revealed the visible individual difference and low urinary recovery of flavonoid metabolites in the excretion of naringin, which may be helpful for further study to understand the in vivo behavior and action mechanism of naringin.
Article
Pharmacology & Pharmacy
Bushra T. AlQuadeib, Fadilah S. Aleanizy, Fulwah Y. Alqahtani, Rehab A. Alshammari, Amal Aldarwesh, Ibrahim Alsarra
Summary: A simple, sensitive, and accurate method for measuring RBX in rat plasma using liquid chromatography-tandem mass spectrometry was developed. The method demonstrated good linearity, precision, and accuracy within the tested concentration range of 25-1000 ng/mL.
SAUDI PHARMACEUTICAL JOURNAL
(2023)
Article
Pharmacology & Pharmacy
Daya Raju Adye, Sachin B. Jorvekar, Upadhyayula Suryanarayana Murty, Subham Banerjee, Roshan M. Borkar
Summary: Analytical sample preparation techniques are crucial for evaluating chemicals in different biological matrices, and the development of extraction techniques is a contemporary trend in bioanalytical sciences.
Article
Biochemical Research Methods
Abigail B. B. Donkor, Carl W. W. White, Heidi J. J. Nick, Brian A. A. Logue
Summary: A rapid, simple, and sensitive HPLC tandem mass spectrometry (MS/MS) method was developed and validated to quantify MESNA in rat plasma. The method demonstrates outstanding limit of detection, excellent linearity, and good accuracy, making it easier for the development of MESNA for therapeutic purposes.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Shasha Jin, Zhihong Li, Qing Yang, Boyu Fang, Xiaoqiang Xiang, Chao Peng, Weimin Cai
Summary: A sensitive and specific chiral liquid chromatography-tandem mass spectrometry method was developed to simultaneously identify the enantiomers of warfarin and hydroxywarfarins. This method has advantages in baseline resolution and analysis processes.
Article
Respiratory System
Weiwei Qin, He Huang, Yuting Dai, Wei Han, Youhe Gao
Summary: This study explored potential urinary biomarkers for CS-induced COPD using LC-MS/MS analysis. Results showed that the urinary proteome sensitively reflected pathological changes in CS-exposed rats, providing valuable clues for exploring COPD biomarkers.
RESPIRATORY RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Junfeng Lu, Chen Chen, Xiaobing Deng, Marvin Sh Mak, Zeyu Zhu, Xixin He, Jinhao Liang, Swetha K. Maddili, Karl W. K. Tsim, Yifan Han, Rongbiao Pi
ACS CHEMICAL NEUROSCIENCE
(2020)
Article
Chemistry, Medicinal
Liping Zhou, Ka-Ying Wong, Sisi Cao, Christina Chui-Wa Poon, Wenxuan Yu, Xiaoli Dong, Karl Wah-Keung Tsim, Man-Sau Wong
Summary: The study found that Danggui Buxue Tang (DBT) can selectively exert estrogenic activities and interact with tamoxifen in tissues, affecting bone, brain, uterus, and breast differently. DBT showed estrogenic effects in a tissue-selective manner, different from tamoxifen, but did not significantly change the effects of tamoxifen in OVX rats.
PHYTOTHERAPY RESEARCH
(2021)
Article
Dermatology
Maggie Suisui Guo, Qiyun Wu, Queenie Wing Sze Lai, Xiaoyang Wang, Panzhu Bai, Kelly Wing Chi Fung, Tina Tingxia Dong, Karl Wah Keung Tsim
Summary: Recent studies have revealed that Self-Growth Colony (SGC) can inhibit melanogenesis by reducing MITF expression, leading to decreased expression of melanogenic enzymes. Additionally, SGC may achieve this effect by activating the ERK signaling pathway.
JOURNAL OF COSMETIC DERMATOLOGY
(2021)
Article
Food Science & Technology
Yi-Teng Xia, Edwin Hok-Chi Cheng, Brody Zhong-Yu Zheng, Qi-Yun Wu, Tina Ting-Xia Dong, Ran Duan, Qi-Wei Qin, Wen-Xiong Wang, Karl Wah-Keung Tsim
Summary: The root of Scutellaria baicalensis has long been used as herbal medicine, while its stem and leaf are considered waste. A study found that water extract from the aerial part of S. baicalensis can replace antibiotics in fish feeding, leading to increased body length and weight in fish. Adding 1% SBA to fish feed altered the composition of amino acids and fatty acids in fish muscle, promoting growth and increasing nutritional value.
FOOD SCIENCE & NUTRITION
(2021)
Article
Dermatology
Maggie Suisui Guo, Xiong Gao, Weihui Hu, Xiaoyang Wang, Tina Tingxia Dong, Karl Wah Keung Tsim
Summary: Scutellarin enhances the regenerative function of SGC in promoting wound healing by binding to VEGF. Co-treatment of scutellarin and SGC on scratched keratinocyte cultures enhances the process of wound healing, and this enhancement is mediated by phosphorylation of VEGFR2 and MAPK signaling.
JOURNAL OF COSMETIC DERMATOLOGY
(2022)
Article
Integrative & Complementary Medicine
Alex Xiong Gao, Tracy Chen-Xi Xia, Marvin Shing-Hung Mak, Kevin Yue Zhu, Tina Ting-Xia Dong, Karl Wah-Keung Tsim
Summary: This study aimed to develop an anti-depressant from a group of food/medicine called medicinal food homology. The herbal mixture of Sesami Semen Nigrum and Longan Arillus showed neurotrophic activity and improved symptoms of depression in cell culture and animal models.
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Wei-Hui Hu, Xiao-Yong Zhang, Ka-Wing Leung, Ran Duan, Ting-Xia (tina) Dong, Qi-Wei Qin, Karl Wah-Keung Tsim
Summary: This study demonstrates that resveratrol, a natural compound, can effectively alleviate vascular distortion, permeability, and hyperplasia in oxygen-induced retinopathy mice. It inhibits the expression of VEGF, VEGFR, and CD31 in the retina and reduces damage caused by oxygen radicals. Resveratrol shows potential therapeutic benefits in angiogenic diseases like retinopathy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Shengying Lin, Xiaoyang Wang, Hongsheng Guo, Niyu Dai, Roy Wai-Lun Tang, Hung Chun Lee, Ka Wing Leung, Tina Ting-Xia Dong, Sarah E. E. Webb, Andrew L. L. Miller, Karl Wah-Keung Tsim
Summary: COVID-19, caused by SARS-CoV-2, has had devastating impacts since its outbreak in 2019, leading to millions of deaths and unprecedented socioeconomic damage. Vaccines against SARS-CoV-2 have limitations and new viral mutations reduce their effectiveness, highlighting the urgent need for potent non-vaccine treatments. Researchers have identified the ethanolic extract of Evodiae Fructus and its component, rutaecarpine, as promising agents that inhibit the activity of 3C-like protease, block viral entry, and reduce inflammatory responses induced by SARS-CoV-2. These findings suggest that Evodiae Fructus extract and rutaecarpine could be considered for clinical applications in treating COVID-19.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Virology
Sheyne S. A. Choi, Ho Hin Chan, Ching Man Chan, Xiaoyang Wang, Sarah E. Webb, Ka Wing Leung, Karl W. K. Tsim, Andrew. L. L. Miller
Summary: Using zebrafish embryos/larvae as an in vivo model, this study explored the mechanism of host cell entry of SARS-CoV-2. The endolysosomal system was found to be involved in viral entry, and TPC2 played a key role in the translocation of the virus through this system. The peripheral sensory organs were identified as a likely site for viral host cell entry.
JOURNAL OF VIROLOGY
(2022)
Article
Neurosciences
Alex Xiong Gao, Tracy Chen-Xi Xia, Lish Sheng-Ying Lin, Tina Ting-Xia Dong, Karl Wah-Keung Tsim
Summary: We aimed to investigate the neurotrophic activities of apigenin (4',5,7-trihydroxyflavone) through its interaction with brain-derived neurotrophic factor (BDNF) and the activation of tyrosine kinase receptor B (Trk B receptor). Our results showed that apigenin and BDNF synergistically enhanced the survival and neurite outgrowth of cultured neurons. Apigenin also potentiated the neurogenesis induced by BDNF and protected against amyloid-beta-induced cytotoxicity and mitochondrial dysfunction. These effects were mediated by the phosphorylation of Trk B receptor. In conclusion, apigenin can enhance the neurotrophic effects of BDNF and may have therapeutic potential in neurodegenerative diseases and depression.
CNS NEUROSCIENCE & THERAPEUTICS
(2023)
Article
Chemistry, Medicinal
Guowei Gong, Yuzhong Zheng, Kumar Ganesan, Qingping Xiong, Karl Wah Keung Tsim
Summary: Danggui Buxue Tang (DBT) can enhance the anticancer capabilities of 5-Fluorouracil (5-FU), a chemotherapy drug, and reduce its side effects, showing promising results in the treatment of colon cancer.
PHYTOTHERAPY RESEARCH
(2023)
Article
Agriculture, Multidisciplinary
Lin Ye, Huodai Chen, Karl Wah Keung Tsim, Xing Shen, Xiangmei Li, Xueling Li, Hongtao Lei, Yunle Liu
Summary: Through mouse experiments, it was found that the gut microbiota plays a crucial role in AFB(1)-induced liver injury, as alterations in gut microbiota lead to colonic barrier dysfunction and liver pyroptosis, resulting in hepatic inflammation.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Chen-Xi Xia, Alex Xiong Gao, Yue Zhu, Tina Ting-Xia Dong, Karl Wah-Keung Tsim
Summary: Seabuckthorn, enriched with flavonoids, showed potential in treating major depressive disorder by improving depressive behaviors, regulating levels of neurotrophins, neurotransmitters, stress-related hormones, inflammation-related cytokines, and modulating gut microbiota.
Article
Biochemistry & Molecular Biology
Alex Xiong Gao, Tracy Chen-Xi Xia, Marvin Shing-Hung Mak, Kenneth Kin-Leung Kwan, Brody Zhong-Yu Zheng, Jian Xiao, Tina Ting-Xia Dong, Karl Wah-Keung Tsim
Summary: The study demonstrated that luteolin can directly interact with nerve growth factor (NGF), enhancing NGF-induced neuronal differentiation and neurofilament expression in cultured PC12 cells. The co-treatment also activated key signaling pathways involved in neuronal growth, mimicking the effects of a high dose of NGF. These findings support the potential development of luteolin as a therapeutic agent for NGF insufficiency-related neurodegenerative diseases.
Article
Chemistry, Multidisciplinary
Anqi Sun, Ben Zhong Tang, Karl Wah-Keung Tsim, Wen-Xiong Wang
Summary: This study focused on the interplay between Zn2+ and nZnO, revealing that nZnO's dissolution potential affects its toxicity in cells. The findings suggest that the contents and properties of nZnO play a more significant role in determining cellular stress than just its dissolution ability.
ENVIRONMENTAL SCIENCE-NANO
(2021)