Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 879, Issue 29, Pages 3203-3207Publisher
ELSEVIER
DOI: 10.1016/j.jchromb.2011.01.033
Keywords
Microchip electrophoresis; Chiral separation; D-Tyrosine; Human plasma; Renal failure
Funding
- National Natural Science Foundations of China [20875019]
- National Basic Research Program of China [2010CB534911]
- Natural Science Foundations of Guangxi Province [0991012Z, 2010GXNSFF013001]
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A rapid and sensitive microchip electrophoresis (MCE) method with laser induced fluorescence (LIF) detection has been developed for the quantification of D-tyrosine (Tyr) in biological samples. The assay was performed using a MCE-LIF system with glass/poly(dimethylsiloxane) (PDMS) hybrid microchip after pre-column derivatization of amino acids with fluorescein isothiocyanate (FITC). Chiral separation of the derivatives was achieved by cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC) using gamma-CD as chiral selector in the running buffer. D/L-Tyr enantiomer was well separated in less than 140s. The limit of detection (S/N = 3) was 3.3 x 10(-8) M. Using the present method, D-Tyr level in human plasma was found to vary significantly from normal humans to patients suffering from renal failure. (C) 2011 Elsevier B.V. All rights reserved.
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