Article
Biochemical Research Methods
Xinyue Liu, Valentina Rossio, Sanjukta Guha Thakurta, Amarjeet Flora, Leigh Foster, Ryan D. Bomgarden, Steven P. Gygi, Joao A. Paulo
Summary: Protein phosphorylation is a central mechanism of cellular signal transduction. This study compares two phosphopeptide enrichment methods and finds that magnetic beads can seamlessly substitute spin columns for global phosphoproteome profiling. The scalable and automation-friendly magnetic bead-based workflow is an equivalent and more flexible enrichment strategy.
JOURNAL OF PROTEOMICS
(2022)
Article
Chemistry, Analytical
Yumi Kwon, Seonjeong Lee, Narae Park, Shinyeong Ju, Sungho Shin, Seongjin Yoo, Hankyul Lee, Cheolju Lee
Summary: Phosphorylation is a key component of cellular signaling cascades, controlling important cellular functions. However, phosphoproteomic analysis is typically limited by the amount of sample required for phosphopeptide enrichment. In this study, a new strategy was developed to increase phosphoproteomic signals from a small amount of sample without phosphoenrichment.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Canhong Zhu, Jiani Wu, Xueting Jin, Yinghua Yan, Chuan-Fan Ding, Keqi Tang, Di Zhang
Summary: In this study, a biomimetic honeycombed affinity chromatography (IMAC) adsorbent was synthesized for enrichment of phosphorylated peptides. The CTSM@AMPA-Ti4+ composites showed high sensitivity and selectivity, and successfully captured 35 phosphorylated peptides from human saliva, providing a promising method for phosphoproteome analysis and disease research.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biochemical Research Methods
Yoko Ino, Eiji Kinoshita, Emiko Kinoshita-Kikuta, Tomoko Akiyama, Yusuke Nakai, Kohei Nishino, Makoto Osada, Akihide Ryo, Hisashi Hirano, Tohru Koike, Yayoi Kimura
Summary: The study evaluated four strategies for phosphopeptide enrichment, with the tip-column strategy showing better efficiency in enriching phosphopeptides from whole-cell lysates in solution, while the Phos-tag agarose beads tip-column strategy yielded the most identified phosphopeptides. The results suggest that multi-enrichment strategies using TiO2 particles and Phos-tag agarose beads are useful for comprehensive phosphoproteomic analysis.
Review
Chemistry, Analytical
Weixiong Huang
Summary: This review summarizes the progress in open tubular ion chromatography (OTIC) from 1981 to 2020, focusing on the challenges of preparation and system requirements, as well as the advancements in key components like conductivity detectors and hydroxide eluent generators that have facilitated the development of OTIC systems. Special attention is given to the suppressed open tubular ion chromatography (SOTIC) system in this comprehensive review.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biochemical Research Methods
Marlene S. Birk, Emmanuelle Charpentier, Christian K. Frese
Summary: An automated Ser/Thr/Tyr phosphopeptide enrichment workflow was established on the Agilent AssayMap platform for studying bacterial phosphorylation. Optimal buffer conditions for TiO2 and Fe(III)-NTA-IMAC cartridge-based enrichment were presented, along with species-specific loading amounts for Streptococcus pyogenes, Listeria monocytogenes, and Bacillus subtilis. Both cartridges enriched a similar set of phosphopeptides, indicating that complementary use does not improve peptide yield.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Biochemical Research Methods
Mohamed Adel Ahmed, Joselito P. Quirino
Summary: The study discusses the in-line sample concentration by micelle to cyclodextrin stacking in open-tubular liquid chromatography with UV detection. Factors influencing MCDS, such as type and concentration of CD, concentration of CTAB, were studied to achieve higher sensitivity and linear range under optimized conditions. Application to real samples showed comparable results to high-performance liquid chromatography-UV method.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Li Qi, Juan Qiao
Summary: Open-tubular capillary electrochromatography (OT-CEC) is an important tool in separation science due to its effective performance and reusability, and the design of smart polymer coatings for OT-CEC remains a challenge. This review focuses on the advancement of research on stimuli-responsive polymeric coatings (SR-PCs) in OT-CEC, including the design and synthesis of stimuli-responsive polymers, fabrication protocols for capillary inner surfaces, strategies for improving separation efficiency, and real-world applications.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Review
Pharmacology & Pharmacy
Xinyu Li, Qianjie Ma, Xiangtai Zheng, Qin Chen, Xiaodong Sun
Summary: Capillary electrochromatography (CEC) has a significant role in chiral separation, and open tubular capillary electrochromatography (OT-CEC) shows promising applications. OT-CEC stationary phases (SPs) developed over the past four years can be categorized into six types, and their characteristics in chiral drug separation are introduced. The applications of OT-CEC in metabolomics, food, cosmetics, environment, and biology, as well as its integration with other instruments like mass spectrometry (MS) and ultraviolet light detector (UV), are also discussed.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2023)
Article
Biochemical Research Methods
Ignacio Arribas Diez, Ireshyn Govender, Previn Naicker, Stoyan Stoychev, Justin Jordaan, Ole N. Jensen
Summary: In this study, different phosphopeptide enrichment techniques were compared, with optimized Zr-IMAC showing improved phosphoproteome coverage and increased phosphopeptide recovery. There was a high degree of overlap in the different enrichment chemistries, but also differences in selectivity and complementarity.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Biochemical Research Methods
Claudia Venditti, Valentina Biagioni, Alessandra Adrover, Stefano Cerbelli
Summary: The impact of transversal flow on separation resolution in Open-Tubular Liquid Chromatography (OTLC) is analyzed. The results show that the presence of transversal flows can reduce the dependence of column length on the Peclet number.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biotechnology & Applied Microbiology
Pratigya Subba, Thottethodi Subrahmanya Keshava Prasad
Summary: Plant systems science research heavily relies on dynamic functional maps of biological substrates and interactions in diverse ecologies. High-resolution mass spectrometry platforms provide insights into the molecular pathways of protein phosphorylation, which is essential for signal transduction in biological systems. Unlike human and animal biology research, experimental options for functional mapping in plant biology are currently limited, with plant phosphoproteomics emerging as a field to address this gap and enable fundamental discoveries.
OMICS-A JOURNAL OF INTEGRATIVE BIOLOGY
(2021)
Article
Biochemical Research Methods
Rui Zong, Xuan Wang, Han Yin, Zijian Li, Chuanlin Huang, Yuhong Xiang, Nengsheng Ye
Summary: A three-dimensional covalent organic framework 3D TpTAM was successfully synthesized via Schiff-base reaction and ultrasound-assisted method, and TpTAM-coated capillary columns were prepared for the separation of fluoroquinolones by capillary electrochromatography with high resolution and reproducibility. The column showed good stability and could be used for more than 100 runs without noticeable changes.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Applied
Tinakorn Kanyanee, Kanlayarat Tianrungarun, Wasin Somboot, Chanida Puangpila, Jaroon Jakmunee
Summary: This work presents the development of a robust, easy-to-use, low-pressure, and cost-effective NS-OTIC approach for small ion determination in drinks. The fabricated device successfully separated mixed cations and mixed anions, with high concentrations of ions directly introduced into the system without dilution. The linear relationship between the peak area and concentration of model ions was obtained, and good repeatability was achieved. Juice and tea samples were analyzed with satisfactory recoveries.
Review
Chemistry, Analytical
Deyber Arley Vargas Medina, Alessandra Timoteo Cardoso, Joao Victor Basolli Borsatto, Fernando Mauro Lancas
Summary: Nano-liquid chromatography (nanoLC) is a primary analytical technique that has gained significant attention across scientific domains. It offers improved chromatographic performance and detectability compared to conventional high-performance LC. Though open tubular LC (OTLC) faces challenges, recent progress has been made in developing and utilizing narrow columns. The review provides an updated outlook on OTLC's developments, instrumental challenges, advancements in column technology, and selected applications.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Forestry
Brian Richardson, Mark O. Kimberley, Carol A. Rolando, Graham W. Coker, Stefan Gous
CANADIAN JOURNAL OF FOREST RESEARCH
(2019)
Article
Forestry
N. Ndlovu, K. Little, L. Titshall, C. A. Rolando
AUSTRALIAN FORESTRY
(2019)
Article
Agronomy
Brian Richardson, Carol A. Rolando, Chanatda Somchit, Christina Dunker, Tara M. Strand, Mark O. Kimberley
PEST MANAGEMENT SCIENCE
(2020)
Article
Plant Sciences
Amin K. Pathan, William Cuddy, Mark O. Kimberly, Kwasi Adusei-Fosu, Carol A. Rolando, Robert F. Park
Article
Chemistry, Analytical
Ziad Mahmoud, Fabrice Bray, Marie Hubert-Roux, Michel Sablier, Carlos Afonso, Christian Rolando
ANALYTICAL CHEMISTRY
(2020)
Article
Biodiversity Conservation
Norman W. H. Mason, Olivia Burge, Robbie Price, Rowan Sprague, John Dymond, Mike Watt, Tessa Roberts, Thomas Paul, Brian Richardson, Carol Rolando, Sarah Wyse, Philip E. Hulme, Pike Stahlmann-Brown, Shaun Awatere, Duane A. Peltzer
Summary: The challenge of designing and implementing long-term management strategies for chronic biological invasions lies in integrating science-based and values-based knowledge sources, as well as developing durable knowledge generation and curation platforms. This paper proposes a transdisciplinary knowledge ecology framework to address this challenge.
BIOLOGICAL INVASIONS
(2021)
Article
Plant Sciences
Carol A. Rolando, Brian Richardson, Thomas S. H. Paul, Chanatda Somchit
Summary: Exotic conifers are rapidly spreading in many regions, leading to detrimental impacts on natural ecosystems and productive environments. Herbicides, especially triclopyr, are vital for managing these invasive species, but there is a lack of information on the amount needed to kill trees of different sizes. Field operations tend to use higher herbicide rates compared to experimental data, indicating potential for substantial reductions in herbicide use with more precise technologies.
INVASIVE PLANT SCIENCE AND MANAGEMENT
(2021)
Article
Chemistry, Analytical
Bastien Venzac, Shanliang Deng, Ziad Mahmoud, Aufried Lenferink, Aurelie Costa, Fabrice Bray, Cees Otto, Christian Rolando, Severine Le Gac
Summary: A systematic study using 16 commercial resins identified a fast and straightforward treatment for 3D-printed structures to support accurate PDMS replication using UV and/or thermal post-curing. Analysis revealed that phosphine oxide-based photo-initiators were poisoning the Pt-based PDMS catalyst, but were eliminated and recombined into high molecular weight species upon UV and/or thermal treatments.
ANALYTICAL CHEMISTRY
(2021)
Article
Agriculture, Multidisciplinary
Kwasi Adusei-Fosu, Carol A. Rolando, Brian Richardson, Rebecca van Leeuwen, Robyn Gaskin, Martin K. -F. Bader, Amin K. Pathan
Summary: This study evaluated the efficacy of fungicides for control of myrtle rust and identified adjuvants that promote spreading of fungicidal active ingredients across the leaf surface. Different inoculation timings significantly influenced the response to infection in Melaleuca excelsa plants.
JOURNAL OF PLANT DISEASES AND PROTECTION
(2021)
Article
Polymer Science
Wissal Adhami, Youssef Bakkour, Christian Rolando
Summary: This study successfully conducted the enzymatic ring opening polymerization of lactones in a flow tubular reactor using CALB lipase immobilized on porous beads, achieving the production of controlled polymers. The results demonstrate that in-situ enzyme catalyzed e-ROP can significantly decrease the residence time needed for the reaction and maintain low dispersity values.
Article
Biochemical Research Methods
Solange Vivier, Aurelien Chepy, Fabrice Bray, Thomas Guerrier, Silvia Speca, Stephanie Flament, Manel Jendoubi, Maite Balden, Christian Rolando, Eric Hachulla, David Launay, Sylvain Dubucquoi, Vincent Sobanski
Summary: Fibroblasts play a crucial role in systemic sclerosis, especially when stimulated with TGF-beta 1. Changes in experimental conditions can affect the proteomic profiles of fibroblasts, particularly when it comes to cell passage and TGF-beta 1 stimulation. Standardization of culture conditions is essential for accurate comparisons in future in vitro studies.
Article
Archaeology
Fabrice Bray, Stephanie Flament, Gregory Abrams, Dominique Bonjean, Christian Rolando, Caroline Tokarski, Patrick Auguste
Summary: This study successfully applied an enhanced proteomic protocol to classify approximately 130,000-year-old mammalian bones, providing new information on extinct species sequences and offering insights for paleontologists.
Article
Chemistry, Multidisciplinary
Marie Yammine, Fabrice Bray, Stephanie Flament, Antoine Picavet, Jean-Marie Lacroix, Emmanuel Poilpre, Isabelle Mouly, Christian Rolando
Summary: Saccharomyces cerevisiae yeast, a fungus with a cell wall protecting it from stress and aiding in communication with the environment, has a complex molecular structure composed of cross-linked polysaccharides and mannoproteins. Studying the molecular characterization of mannoproteins is crucial for their functional properties, relying on optimal isolation and preparation of the cell wall fraction.
Article
Biochemistry & Molecular Biology
Houssein Zhour, Fabrice Bray, Israa Dandache, Guillaume Marti, Stephanie Flament, Amelie Perez, Maelle Lis, Llorenc Cabrera-Bosquet, Thibaut Perez, Cecile Fizames, Ezekiel Baudoin, Ikram Madani, Loubna El Zein, Anne-Alienor Very, Christian Rolando, Herve Sentenac, Ali Chokr, Jean-Benoit Peltier
Summary: Diazotrophic bacteria isolated from the rhizosphere of a wild wheat ancestor were found to be efficient Plant Growth-Promoting Rhizobacteria (PGPR) in promoting the growth of elite wheat cultivars. They increased nitrogen levels in nitrogen-starved plants and influenced root development. Analysis of bacterial exudates revealed a complex set of secondary metabolites, including phytohormone mimetics and inter-kingdom signaling molecules.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agricultural Engineering
B. Richardson, C. Rolando, M. Kimberley
TRANSACTIONS OF THE ASABE
(2020)