Article
Biochemical Research Methods
Eline Verscheure, Matteo Creta, Dorian Vanneste, Jeroen Vanoirbeek, Meziane Zakia, Taleb Abdesselam, Robin Lebegge, Katrien Poels, Manosij Ghosh, Radu-Corneliu Duca, Lode Godderis
Summary: A sensitive method for simultaneous detection and quantification of antineoplastic drugs in urine was developed in this study, allowing for effective assessment of occupational exposure among healthcare workers.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Biochemical Research Methods
Shuailong Chen, Xuan Li, Shijia Liu, Longshan Zhao, Wenyong Zhang, Zhili Xiong, Hemi Luan
Summary: A rapid, sensitive, and high-throughput isotope dilution liquid chromatography-negative ion electrospray tandem mass spectrometry (IDLC-nESI-MS/MS) method was developed for accurate quantification of multiple ceramides. This method demonstrated good linearity and recovery, and could be applied for studying ceramide biology and disease diagnosis.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Martina Franzin, Rachele Ruoso, Rossella Del Savio, Eugenia Akhavan Niaki, Aba Pettinelli, Giuliana Decorti, Gabriele Stocco, Riccardo Addobbati
Summary: The use of therapeutic cannabis preparations is growing in Friuli Venezia Giulia. However, there is a need for standardization and quality control measures for cannabis oil, which is used to treat various disorders. Gas chromatography coupled to mass spectrometry (GC-MS) is an effective method for quantifying cannabinoids in cannabis oil.
Article
Medical Laboratory Technology
Jordi Farre-Segura, Neus Fabregat-Cabello, Chiara Calaprice, Laurent Nyssen, Stephanie Peeters, Caroline Le Goff, Etienne Cavalier
Summary: A rapid and specific LC-MS/MS method for glucagon quantitation has been developed and validated, showing stability and accuracy in comparison to other immunoassay methods. This method could potentially establish a standardized approach for glucagon analysis in routine care settings.
CLINICA CHIMICA ACTA
(2021)
Article
Biochemical Research Methods
Monika Beszterda, Rafal Franski
Summary: This study aimed to analyze the ESI-MS behavior of flavonoid 5-O-glucosides in cherry tree bark, compare it with their positional isomers, and utilize the susceptibility to hydrolysis for identification. The results showed that under acid conditions, flavonoid 5-O-glycosides were completely hydrolyzed, giving distinctive ions in positive and negative ion modes for effective differentiation from their positional isomers.
PHYTOCHEMICAL ANALYSIS
(2021)
Article
Chemistry, Analytical
C. Louwrens Braal, Mei H. Lam, Tineke Rienks, Claudia J. van Tilborg, Wendy Heuts, Joan B. Heijns, Monique E. M. M. Bos, Ron H. J. Mathijssen, Peter de Bruijn, Stijn L. W. Koolen
Summary: A reliable and specific LC-MS/MS method was developed for determining ribociclib in both DBS samples and plasma. The method met FDA guidelines for validation and showed a strong correlation between ribociclib concentrations in DBS and plasma. This study demonstrates the potential of using DBS for monitoring ribociclib levels in breast cancer patients.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Automation & Control Systems
Adam J. Taylor, Dimitrios Tsikritsis, Alex Dexter, Amy Burton, Josephine Bunch, Natalie A. Belsey
Summary: Rapid chemical characterization methods can assess and classify differences in solid oral dosage forms, helping to identify off-brand or counterfeit products.
JOURNAL OF CHEMOMETRICS
(2022)
Article
Biochemical Research Methods
Monica Romagnoli, Andrea Scarparo, Martina Catani, Biagio Gianni, Luisa Pasti, Alberto Cavazzini, Flavio A. Franchina
Summary: Water contamination by pesticide residues is a concern due to their widespread use. In this study, a multiresidue method combining solid-phase extraction and multidimensional gas chromatography coupled to mass spectrometry was used to analyze 53 pesticides and their metabolites in water samples from pesticide-susceptible agricultural areas. The method was validated and applied to real-world water samples, showing comparable results with a routine method. Regulatory directives have set maximum concentration limits for individual pesticides and total pesticide residues in water samples.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Food Science & Technology
Thomas J. McGrath, Giulia Poma, Jasper Bombeke, Franck Limonier, Els Van Hoeck, Laure Joly, Adrian Covaci
Summary: This work optimized an analytical method for quantifying short- and medium-chained chlorinated paraffins in various food matrices, and validated the method's applicability through repeated analysis of samples fortified with SCCPs and MCCPs. The method showed good accuracy, high precision, and suitability for different food matrices.
Article
Chemistry, Analytical
C. Bottinelli, R. Nicoli, F. Bevalot, N. Cartiser, C. Roger, K. Chikh, T. Kuuranne, L. Fanton, J. Guitton
Summary: The study aimed to develop the first validated method for analyzing human insulin and its synthetic analogues, primarily for plasma and post-mortem sera. Different extraction processes were evaluated for the lowest limit of detection for target analogues, and the limits of quantification were validated in plasma and post-mortem sera. Results showed that at the LOQ level, accuracy was between 70 and 130%, with precision lower than 30%.
Article
Food Science & Technology
Jensen Cherewyk, Taylor Grusie-Ogilvie, Barry Blakley, Ahmad Al-Dissi
Summary: This study validated a new method using ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) to quantify 12 epimers of ergot alkaloids in wheat. The method showed low LOD and LOQ values, allowing for sensitive detection of ergot epimers. The study also found differences between the R and S-epimers, which has rarely been documented in previous research.
Article
Chemistry, Applied
Zi-Rong Tseng, Chieh-Chun Wei, San-Yuan Wang, Hsiao-Wei Liao
Summary: The study successfully employed untargeted profiling to screen unexpected contaminants in commercial milk, identifying the presence of Irgacure-907. This approach can potentially reveal other unknown food contaminants in various food matrices in the future, benefiting public health worldwide.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2021)
Article
Biochemical Research Methods
Sarah Schleicher, Gavin O'Connor, Petra Mischnick
Summary: The methyl distribution of methyl cellulose (MC) is commonly studied by analyzing cello-oligosaccharides (COS) with ESI-MS after perdeuteromethylation. Isotope labeling with (CH3)-C-13 instead of CD3-etherified COS can provide more precise and accurate results. (CH3)-C-13 is more robust in determining the methyl distribution of MCs.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Jan Thomann, Laura Ley, Aaron Klaiber, Matthias E. Liechti, Urs Duthaler
Summary: In this study, a bioanalytical method was developed for the rapid quantification of mescaline and its metabolites in human plasma. The method showed reliability and ease of use, making it suitable for forensic applications as well as investigating the clinical pharmacokinetics of mescaline.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Chemistry, Medicinal
Margaux Fresnais, Siwen Liang, Marius Breitkopf, Joshua Raoul Lindner, Emmanuelle Claude, Steven Pringle, Pavel A. Levkin, Konstantin Demir, Julia Benzel, Julia Sundheimer, Britta Statz, Kristian W. Pajtler, Stefan M. Pfister, Walter E. Haefeli, Juergen Burhenne, Remi Longuespee
Summary: Desorption/ionization-mass spectrometric methods are advantageous for the analysis of solid biological matrices. The development of drug quantification assays using DESI has been challenging, but new prototype source setups show promise for targeted quantitative analyses.