Article
Biochemical Research Methods
Jincan Shen, Fengjuan Zhao, Pingping Zhu, Fengqi Wu, Xinyi Chen, Haining Kang, Zhenfeng Yue
Summary: A method for the direct determination of nosiheptide by LC-MS/MS in animal tissues was developed. The method was found to be more simple, convenient, and sensitive compared to previous indirect methods. It was successfully applied for the determination of nosiheptide residue in medicated chicken samples.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Agriculture, Multidisciplinary
Sinah Reiter, Andreas Dunkel, Corinna Dawid, Thomas Hofmann
Summary: Bile acids are increasingly investigated as markers for various diseases in humans and animals. A high-throughput workflow was used for bile acid analysis in animal studies, mapping complex bile acid profiles in tissues and body fluids. This method can also be used for screening food and studying the bioavailability of bile acids in the GI tract.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Chemistry, Analytical
Yanhua Chen, Qianlun Pu, Fengting Yu, Xian Ding, Yu Sun, Qinglan Guo, Jiangong Shi, Jianjun Zhang, Zeper Abliz
Summary: In this study, a high-throughput quantitative method was developed to simultaneously analyze neurotransmitters and their metabolites in various samples from rats. The method was used to investigate the neurochemical mechanism of a sedative-hypnotic candidate drug. The results showed that the drug administration affected the correlations between neurotransmitters and disrupted the sleep-wake balance.
Article
Biochemical Research Methods
Kacey D. Cliburn, Marilyn A. Huestis, Jarrad R. Wagner, Philip M. Kemp
Summary: A liquid chromatography-tandem mass spectrometry method was developed and validated for quantifying cannabinoids in postmortem specimens, showing accuracy and stability in measuring various cannabinoids.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biochemistry & Molecular Biology
Xin Shen, Gaofu Li, Libin Wang, Huijin Yu, Lei Zhou, Huifang Deng, Ningning Wang, Chengcai Lai, Wei Zhou, Yue Gao
Summary: This study evaluated the pharmacokinetics of acipimox in rats under simulated high altitude hypoxia conditions. A sensitive LC-MS/MS method was established for quantification of acipimox in rat plasma and tissue homogenate. The results showed significant differences in the pharmacokinetics parameters of acipimox between normoxic and hypoxic rats. Changes in lipid metabolism-related proteins and free fatty acid levels were also observed.
Article
Chemistry, Analytical
Xiaoling Su, Deying Chen, Liang Li, Lanjuan Li
Summary: The study demonstrates that perfusion can significantly alter the liver metabolome, recommending the use of non-perfused liver for comprehensive liver metabolomics analysis for high coverage.
ANALYTICA CHIMICA ACTA
(2021)
Article
Food Science & Technology
Borja Munoz-Solano, Elena Gonzalez-Penas
Summary: Mycotoxins are a major concern for food safety, as their exposure in animals can lead to health problems, economic losses, and contamination of animal-derived foods. This study used a revalidated methodology to analyze mycotoxins in animal plasma samples, finding that the prevalence of STER was high but levels were low in the analyzed feed. Animal biomonitoring can be a useful tool in assessing mycotoxin exposure, but more research is needed on appropriate biomarkers and analytical methods.
Article
Veterinary Sciences
Yong Wu, Xue-Ming Long, Gao-Feng Liu, Xia Bai, Zhi-Liang Sun, Zhao-Ying Liu
Summary: The study aimed to investigate tissue residue depletion in pigs fed G. elegans powder. Two quantitation methods, including a precise method and a semi-quantitation method, were developed and validated for the analysis of multiple components in pig tissues. The results showed that G. elegans alkaloids were widely distributed in pig tissues, and potential residue markers and target tissues were identified. The findings provide important scientific evidence for evaluating the safety of animal-derived food from G. elegans.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Environmental Sciences
Ruiguo Wang, Yuan Huang, Shujun Dong, Peilong Wang, Xiaoou Su
Summary: Animal-derived food plays a significant role in human exposure to bisphenol compounds, with limited studies on the source of bisphenols in animal feed. This study examined the occurrence of BPs in animal feed packaging and their migration into feed, confirming that BPA can migrate from plastic packaging into solid feed. Results show that contact time and initial BP concentration affect the migration extent, expanding knowledge on the origin of BPs in the food chain.
Article
Chemistry, Analytical
Jianmei Wang, Jinmin Zhang, Ngoc Thao Duyen Nguyen, Yu-An Chen, Jer-Tsong Hsieh, Xiaowei Dong
Summary: This study developed a new HPLC method to measure IR780 concentration in pharmaceutical formulations and a new LC-MS/MS method to measure IR780 concentration in tissue samples, particularly in liver and lung. These quantitative methods provide reliable measurements of IR780 in both formulations and biological samples, facilitating research on IR780 as a theranostic agent for cancer therapy and imaging.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Environmental Sciences
Gwenaelle Lavison-Bompard, Julien Parinet, Karelle Huby, Thierry Guerin, Chanthadary Inthavong, Marine Lambert
Summary: The study investigated the contamination levels of Chlordecone (CLD) in 200 bovines from Martinique and Guadeloupe, revealing a broader concentration range of CLD in the liver compared to fat and muscle. Statistical processing indicated correlations between CLD concentrations in the three studied tissues.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Biochemistry & Molecular Biology
Vadim V. Yanshole, Arsenty D. Melnikov, Lyudmila V. Yanshole, Ekaterina A. Zelentsova, Olga A. Snytnikova, Nataliya A. Osik, Maxim V. Fomenko, Ekaterina D. Savina, Anastasia V. Kalinina, Kirill A. Sharshov, Nikita A. Dubovitskiy, Mikhail S. Kobtsev, Anatolii A. Zaikovskii, Sofia S. Mariasina, Yuri P. Tsentalovich
Summary: The Animal Metabolite Database (AMDB) is a freely accessible database that allows browsing and comparison of quantitative metabolomics data, with a focus on metabolite concentrations. It also serves as a platform for the metabolomics community and is constantly expanding its data content and species range. AMDB has significant importance in various scientific fields.
Article
Biochemistry & Molecular Biology
Vineet Joshi, Yashpal S. Chhonker, Dhruvkumar Soni, Kelly C. Cunningham, Derrick R. Samuelson, Daryl J. Murry
Summary: A novel LC-MS/MS method was developed to quantify indole concentrations in mouse plasma and tissues, which could serve as a biomarker for several diseases. This method separated and measured the ion state of indole and an internal standard, allowing for accurate determination of indole concentrations range in different samples. This study contributes to the understanding of the role of indole as a biomarker and its implications in various disease states.
Article
Agriculture, Dairy & Animal Science
Francesco Cerasoli, Michele Podaliri Vulpiani, Giorgio Saluti, Annamaria Conte, Matteo Ricci, Giovanni Savini, Nicola D'Alterio
Summary: This study evaluated the stress levels in horses with different management and activities by analyzing cortisol levels in horsehair. The results showed that free-ranging horses had significantly higher cortisol levels compared to stabled and working horses. This suggests that factors such as management, environment, and behavior may influence the stress levels in horses and should be further investigated.
Article
Biochemistry & Molecular Biology
Yiyue Yang, Ming Yang, Tong Zhao, Lingyi Pan, Li Jia, Lufei Zheng
Summary: This study describes the variation in residue behavior of fluopyram in soil, carrot root, and carrot leaf samples after the application of fluopyram. The results show that the residue levels in edible parts were higher than the maximum residue limit in China when fluopyram was applied by root irrigation, while most of the fluopyram dissipated from the surface of carrot leaves when applied by foliar spray.
Article
Environmental Sciences
Lei Zhang, Jiahuai Zhang, Sai Fan, Yuxin Zhong, Jingguang Li, Yunfeng Zhao, Song Ni, Jiaying Liu, Yongning Wu
Summary: This case-control study investigated the associations between urinary concentrations of bisphenols and the risk of papillary thyroid cancer (PTC). The results showed significant positive associations between bisphenol F (BPF) concentration and PTC risk, but negative associations between bisphenol A (BPA) and bisphenol S (BPS) concentrations and PTC risk in the overall population. However, the significant associations were only observed in non-smoking women after stratification by age and smoking.
Article
Engineering, Environmental
Feng Han, Jiaying Liu, Yuxin Wang, Jingguang Li, Bing Lyu, Yunfeng Zhao, Yongning Wu
Summary: The mechanism of blood to milk transfer of PFOS and Cl-PFESAs was studied using 145 paired maternal serum and human milk samples. The transfer efficiency was found to be influenced by the physicochemical properties and primary components of human milk. Results suggest that passive diffusion through the paracellular route may be the main transfer pathway for PFOS and Cl-PFESAs from blood to milk.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Engineering, Environmental
Yizhong Shen, Yunlong Wei, Xiang Gao, Chao Nie, Jianlong Wang, Yongning Wu
Summary: Scientists successfully synthesized a novel blue fluorescence nanozyme CeO2@TPE with high peroxidase-like activity. By embedding aggregation-induced emission (AIE) tetraphenylethene (TPE) into hollow CeO2 nanospheres and combining ethanol oxidase (AOX) and o-phenylenediamine (OPD), they developed an enzymatic cascade activation ratiometric fluorescence-colorimetric dual-mode system for detecting methyl mercaptan (CH3SH). This study provides a new insight into designing target-responsive enzymatic cascade activation systems and enables real-time point-to-point detection of environmental health.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Engineering, Environmental
Xuezhen Zhao, Bing Lyu, Lei Zhang, Jingguang Li, Yunfeng Zhao, Yongning Wu, Zhixiong Shi
Summary: Based on the 6th China Total Diet Study, both legacy and novel brominated flame retardants (BFRs) were found in animal-derived foods across China. The levels and estimated dietary intakes (EDIs) of most BFRs showed a significant decrease compared to previous studies conducted from 2007 to 2011. Meat consumption was identified as the primary source of dietary BFR intake. However, daily dietary intakes of BFRs were still unable to cause significant health risks to the general population in China.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Infectious Diseases
Danxia Gu, Zelin Yan, Chang Cai, Jiaping Li, Yanyan Zhang, Yuchen Wu, Jiaxing Yang, Yonglu Huang, Rong Zhang, Yongning Wu
Summary: In this study, the efficacy of three different methods for the rapid detection of carbapenemases (KPC, NDM, IMP, OXA-48, and VIM) was evaluated. The NG-test Carba 5, colloidal gold immunoassay (CGI) test, and Xpert Carba-R assay showed reliable performances in carbapenemase detection, with Xpert Carba-R being recommended for the detection of KPC variants.
Article
Chemistry, Applied
Feifei Huang, Lei Zhang, Moqin Zhou, Jianwen Li, Qing Liu, Bo Wang, Ke Deng, Pingping Zhou, Yongning Wu
Summary: Polycyclic aromatics hydrocarbons (PAHs) are commonly found in foods and the environment and have carcinogenic and mutagenic potential. A study in China determined the concentrations of 16 PAHs in commonly consumed food items. Edible vegetable oils had the highest concentration of PAHs, while fruits had the lowest. PAH4 was identified as a suitable indicator for PAH contamination, and the overall dietary exposure for PAHs in the Chinese population was estimated to be within safe limits.
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2023)
Article
Agriculture, Multidisciplinary
Chi Zhang, Heli Li, Huijing Shen, Bing Lyu, Shaohua Li, Jingguang Li, Yunfeng Zhao, Dawei Chen, Yongning Wu
Summary: This study is the first nationwide study in China on the chronic health risks of chlorothalonil and its metabolite (4-OH-chlorothalonil) for Chinese adults and breastfed infants. The study found that chlorothalonil and 4-OH-chlorothalonil were detected in dietary samples, with higher levels in Northwest China and Shandong regions. Other exposure routes besides dietary intake were also identified. The study concluded that the chronic health risks from dietary exposure to chlorothalonil and 4-OH-chlorothalonil are low for Chinese adults and breastfed infants.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Nutrition & Dietetics
Lan Ma, Huijing Shen, Xiaohong Shang, Shuang Zhou, Bing Lyu, Xin Zhao, Jingguang Li, Yunfeng Zhao, Yongning Wu
Summary: The recent total diet study comprehensively evaluated the intake of nutrient elements in the general Chinese population. Plant foods were the main source of both macro-elements and trace elements. The average intake of Na, Ca, Mg, Zn, and Se did not meet the health guidance values, while the intake of K, P, Mn, Fe, Cu, Mo, and Cr fell within a reasonable range. A dietary imbalance was identified in the Na/K ratio and Ca/P ratio. This study highlights the importance of salt reduction and dietary structure optimization for the population.
Article
Nutrition & Dietetics
Hangyu Yu, Rui Wang, Yunfeng Zhao, Yan Song, Haixia Sui, Yongning Wu, Hongjian Miao, Bing Lyu
Summary: The sixth Total Diet Study (TDS) of China conducted a nationwide study to evaluate the health effects of MSG. The study analyzed 168 samples from seven food categories of the typical Chinese daily diet to determine MSG content, consumption patterns, and associated risks. The study found that the highest MSG level in the Chinese population's daily diet was 8.63 g/kg. By combining food consumption and MSG content measurements, the estimated MSG intake for the general population of China was 17.63 mg/kg bw/d, while the apparent consumption alone resulted in an overestimated intake of 40.20 mg/kg bw/d due to the omission of MSG loss during cooking. The study also compared and analyzed MSG content, food category contributions, and ingestion levels across different nations to provide a global perspective. Furthermore, a realistic, logical, and precise risk assessment protocol for daily MSG intake was developed in this article.
Article
Allergy
Hua Feng, Jiangdong Zhou, Yuanan Lu, Qian Zhao, Zifei Yang, Xiujuan Xiong, Nan Luo, Yan Chen, Lianglu Wang, Yongning Wu
Summary: The prevalence of self-reported food allergy among adults in China is approximately 4.0%, with 3.1% in men and 4.8% in women. The most common allergic symptom is skin reaction, reported by 63.9% of participants with self-reported food allergy. Shrimp, mollusks, and mango are the most common allergenic foods, accounting for 39.8%, 20.8%, and 18.7% of cases respectively. Gender, age, and other allergic conditions are associated factors with food allergy in adults.
WORLD ALLERGY ORGANIZATION JOURNAL
(2023)
Article
Chemistry, Applied
Yajie Wang, Jing Cao, Guanzhao Wang, Tao Wei, Kexin Hu, Wenqi Yi, Pingyang Zeng, Huifang Li, Yongning Wu, Qinghua He
Summary: Three ZIFs materials were synthesized and applied to the adsorption and detoxification of gossypol in cottonseed oil. The materials exhibited good crystal structure, thermal stability, and high specific surface area. The adsorption processes followed pseudo-second-order kinetics and were better described by the Langmuir model. The ZIFs materials showed high detoxification rates in both spiked and real cottonseed oil samples.
Article
Food Science & Technology
Hui -Ming Chen, Quan Zhou, Li-Jun Huang, Jun Lin, Jia-Fei Liu, Zi-Yong Huang, Rong-Lin Zhang, Jing-Jing Wang, Yong Zhao, Yong-Ning Wu, Xing-Fen Yang, Wei-Liang Wu
Summary: This study aimed to evaluate the effects of curcumin-mediated photodynamic treatment (PDT) on the shelf-life quality of ready-to-eat salmon fillets. The results showed that PDT had outstanding bacterial inhibition and extended the shelf-life of salmon fillets. Additionally, PDT was able to maintain the physicochemical indicators and freshness of the salmon fillets, and improve the sensory scores.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Agriculture, Multidisciplinary
Guojian Wu, Abudoushukeer Dilinaer, Peng Nie, Xin Liu, Zhi Zheng, Pengjie Luo, Wenjuan Chen, Yongning Wu, Yizhong Shen
Summary: Developing efficient meat freshness assays is crucial for ensuring food safety. In this study, a bimetallic nanozyme Fe@CeO2 with high peroxidase-like activity was synthesized, and a self-supplying enzymatic cascade catalytic system was developed for colorimetric and photothermal detection of meat freshness. The assay showed both reliability and sensitivity, providing new insights for onsite visual monitoring of meat freshness.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Jinde Liu, Qiao Rong, Chunxiao Zhang, Ali Tariq, Lin Li, Yongning Wu, Feifei Sun, Michal Tomczyk
Summary: It has been reported that Mori Folium (MF) and Eucommiae Cortex (EC) exhibit pharmacological effects in the treatment of immunosuppression. This study aimed to explore the mechanism of action of MF and EC against immunosuppression using network pharmacology, molecular docking, molecular dynamics simulations, and animal experiments. The results obtained from network pharmacology identified important components, hub targets, and signaling pathways involved in the treatment of immunosuppression. Molecular docking and dynamics simulations confirmed the stable binding of certain critical components to hub targets. Animal experiments also demonstrated the significant effect of MF and EC in treating immunosuppression. These findings suggest that MF and EC may have a multi-component, multi-target, and multi-pathway mode of action in treating immunosuppression.
Article
Biochemistry & Molecular Biology
Luyao Zhang, Jialu Liu, Bufan Xu, Di Wu, Yongning Wu, Guoliang Li
Summary: In this study, the effects of exposure to different concentrations of beta-carbolines norharman and harman on the survival rate and behaviors of C. elegans were assessed. The changes in antioxidant system, neurotransmitter levels, and acetylcholinesterase activity were evaluated. RNA-seq analysis identified potential molecular mechanisms underlying the toxic effects of norharman and harman. The results suggest that long-term exposure to low doses of beta-carbolines in food poses a risk of neurotoxicity.