Article
Engineering, Chemical
Vanya Rangelov Kozhuharov, Kalin Ivanov, Diana Karcheva-Bahchevanska, Natalia Prissadova, Stanislava Ivanova
Summary: The use of dietary supplements has increased significantly, but a significant portion of these products contain undeclared pharmacologically active compounds, which could lead to serious side effects. One of the most commonly detected undeclared compounds is sibutramine, a prohibited substance considered doping by WADA. A GC-MS method was developed to detect and quantify sibutramine in dietary supplements, with good validation and detection limits. This method can contribute to quality control and the prevention of health risks associated with adulterated dietary supplements.
Article
Biochemical Research Methods
Chengjun Guo, Ning Zhang, Xiaoli Zhang, Mingfeng Chi, Dongren Liu, Jing Zhang
Summary: Since higenamine was included in the WADA prohibited list, the detection of this substance in various plants has raised concerns among doctors about prescribing traditional Chinese medicine to athletes. This study determined the concentration-time curves of higenamine in urine after single or multiple administrations of lotus seed powder. The results showed that higenamine could be detected in urine after administration and the concentration exceeded the WADA limit in some samples, indicating a high risk for athletes.
DRUG TESTING AND ANALYSIS
(2022)
Article
Biochemical Research Methods
Thomas Piper, Sonja Heimbach, Martin Adamczewski, Mario Thevis
Summary: The steroid profile is used in sports drug testing to detect misuse of endogenous steroids, with individual reference ranges compared to population-based thresholds via the Athlete Biological Passport. Different doping agents were investigated for their effects on the steroid profile, using various tests coupled with analytical approaches to understand compound impact, although predicting specific influences remains challenging.
DRUG TESTING AND ANALYSIS
(2021)
Article
Biochemical Research Methods
Aoalheiour Dora Albertsdottir, Wim Van Gansbeke, Peter Van Eenoo, Michael Polet
Summary: The detection of anabolic androgenic steroids (AAS) is frequent among rule-breaking athletes. The long-term metabolites, particularly non-hydrolysed sulphated metabolites, have gained attention for their extended detection time. Liquid and gas chromatography-mass spectrometry (LC- and GC-MS) have been used to investigate their potential. However, the most promising metabolite on one technique may not exhibit the same behavior on the other. Consequently, a comparison was carried out to identify the most likely long-term sulphated metabolites of metenolone, mesterolone, and 17-alpha-methyltestosterone on four mass spectrometric instruments.
DRUG TESTING AND ANALYSIS
(2023)
Article
Chemistry, Applied
Bing Lyu, Xiaoyan Zhang, Jingguang Li, Lei Zhang, Yuxin Zhong, Yongning Wu
Summary: The present study evaluated two GC-MS/MS instruments with different ion sources for detecting trace levels of PCDD/Fs in various foods. The GC-MS/MS methods showed good sensitivity, repeatability, and reproducibility, and the results were comparable to those obtained with GC-HRMS.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Chemistry, Analytical
Chao Han, Chaoqun Huang, Qinting Jiang, Li Chen, Yi Li, Chengjie Lou, Hailing Lu, Yan Shen
Summary: A novel and accurate method for the determination of folpet and phthalimide residues in tea has been developed, using solid-phase extraction combined with gas chromatography-tandem mass spectrometry. The method proposes converting all folpet to phthalimide prior to sample extraction and purification, and utilizes an isotopic internal standard for quantification. The study confirms the accurate determination of phthalimide content and its conversion to folpet content.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Applied
Mu Zhao, Ting Li, Fan Yang, Xinyue Cui, Tingting Zou, Huanlu Song, Ye Liu
Summary: This study characterized the composition of volatile aroma compounds and key aroma-active compounds in dried Hanyuan Zanthoxylum bungeanum. The results identified several key aroma-active compounds such as myrcene and limonene.
Article
Chemistry, Applied
Xuejie Li, Xiangquan Zeng, Huanlu Song, Yu Xi, Yan Li, Bowen Hui, He Li, Jian Li
Summary: GC x GC-O-TOF-MS and GC-IMS have been increasingly used in complex food flavor analysis, but few studies have applied these techniques to identify the aroma components of tomato pastes. This study comprehensively characterized the aroma profiles of cold and hot break tomato pastes using SAFE-GC-O-MS, SAFE-GC x GC-O-TOF-MS, and HS-GC-IMS for the first time. A total of 274 volatile compounds were identified, which is far more than previously reported.
Article
Chemistry, Applied
Yanqin Yang, Jialing Xie, Qiwei Wang, Yuliang Deng, Li Zhu, Jiayi Zhu, Haibo Yuan, Yongwen Jiang
Summary: This study comprehensively characterized the volatile and non-volatile compounds during black tea processing using volatolomics and UHPLC-Q-Exactive/MS analysis. The results showed that these metabolites changed continuously throughout the processing process, with significant changes during the withering stage. A total of 178 volatile metabolites and 103 non-volatile metabolites were identified, and 11 volatile components were identified as key aroma active components responsible for sweet aroma in finished black tea.
Article
Chemistry, Analytical
Ananya Kongsuwan, Sariktiya Chuenjitt, Cheng Ho Phua, Sangay Wangchuk, Jenjira Saichanapan, Kasrin Saisahas, Kritsada Samoson, Asamee Soleh, Kiattisak Promsuwan, Warakorn Limbut
Summary: A new electrode material, Na/P-CNTs, was synthesized via a hydrothermal reaction and used for modifying an electrode in an electrochemical sensing system. The nanocomposite showed enhanced electro-catalytic activity and electron transfer, which resulted in effective oxidation of sibutramine. The developed electrode achieved a wide linear range, low detection limit, and good analytical performances for sibutramine determination.
MICROCHEMICAL JOURNAL
(2023)
Article
Energy & Fuels
Luise Carvalho Dias, Karina Santos Garcia, Antonio Fernando de Souza Queiroz, Maria Elisabete Machado
Summary: This study presents and validates a novel GC-MS/MS method for the determination of nitrogen-containing heterocyclic aromatic compounds in crude oils, showcasing its potential for fast, easy, and efficient quantitative analysis of N-markers.
Article
Food Science & Technology
Chao Han, Beizhen Hu, Chaoqun Huang, Wenhua Zhang, Huizhen Wu, Caiqin Liu, Qiyong Chen, Yan Shen
Summary: A new analytical method involving gas chromatography-tandem mass spectrometry (GC-MS/MS) was developed for the determination of morpholine residues in fruit and fruit juice samples. The method showed good precision and accuracy and can be widely applied in food safety monitoring.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Environmental Sciences
Zi-Feng Zhang, Jia-Cheng Chen, Ya-Xian Zhao, Lu Wang, Yu-Qian Teng, Ming-Hong Cai, Yan-Hui Zhao, Anatoly Nikolaev, Yi-Fan Li
Summary: Polycyclic aromatic hydrocarbons (PAHs) are toxic organic pollutants that have been widely detected in various regions and environmental matrices. However, not all PAHs are usually detected, and there is no established method to systematically analyze hundreds of PAHs. In this study, a gas chromatography-tandem triple-quadrupole mass spectrometry (GC-MS/MS) method was developed and successfully applied to identify and quantify 123 PAHs in marine samples.
Review
Biochemical Research Methods
Rachel A. Hand, Thomas Bassindale, Nicholas Turner, Geraint Morgan
Summary: Anti-doping analysis requires high accuracy and precision, with current methods such as selective monitoring by quadrupole mass spectrometry facing issues. Comprehensive 2D chromatography offers improved separation capabilities.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Multidisciplinary Sciences
Lubna Rahman, Asma Mukhtar, Sajjad Ahmad, Lutfur Rahman, Muhammad Ali, Muhammad Saeed, Zabta Khan Shinwari
Summary: The current study aimed to isolate and identify bioactive compounds from endophytic bacteria. Molecular docking and biological assays were used to investigate the interactions between the compounds and bacterial cells. The study discovered antibacterial compounds and provided important information on drug-receptor interactions, which can be useful in novel drug discovery.
Article
Chemistry, Applied
Glaucia Cristina Manco da Costa Bolson, Ananda da Silva Antonio, Iuri Bezerra de Barros, Gustavo Ramalho Cardoso dos Santos, Henrique Marcelo Gualberto Pereira, Larissa Silveira Moreira Wiedemann, Valdir Florencio da Veiga-Junior
Summary: Research evaluated the chemical composition of endemic Amazonian freshwater sponge species, revealing them as rich sources of fatty acids and sterols, which could serve as potential chemical markers for further targeted metabolomic studies.
NATURAL PRODUCT RESEARCH
(2021)
Article
Toxicology
Estefany Prado, Rebecca Rodrigues Matos, Geovana Maria de Lima Gomes, Clarisse Baptista Lima de Sa, Isabelle Karine Costa Nunes, Carina de Souza Anselmo, Adriana Sousa de Oliveira, Luciana Silva do Amaral Cohen, Denilson Soares de Siqueira, Marco Antonio Martins de Oliveira, Joao Carlos Laboissiere Ambrosio, Gabriela Vanini Costa, Francisco Radler de Aquino Neto, Monica Costa Padilha, Henrique Marcelo Gualberto Pereira
Summary: The study aimed to identify in vivo phase I metabolites of five psychoactive substances using a zebrafish water tank experimental setup and high-resolution mass spectrometry. Results showed that alpha-PVP, N-ethylpentylone, ethylone, and 4-CDC can be transformed into phase I metabolites, while methylone appears to be a potent CYP450 inhibitor in zebrafish. The approach was found to be simple, cost-effective, and efficient for studying the metabolism of synthetic cathinones.
FORENSIC TOXICOLOGY
(2021)
Article
Biochemical Research Methods
Julio M. X. Amaral, Monica C. Padilha, Silvana Chagas, Julien S. Baker, Carrie Mullen, Leonardo Vieira Neto, Francisco R. Aquino Neto, Marcelo S. Cruz
Summary: This study details the treatment of a man who attempted suicide after experiencing symptoms of anxiety and aggressiveness associated with the use of AAS. Regular use of fluoxetine was effective in preventing anxiety, depression, aggressiveness, and suicide ideation, even in the presence of AAS. Urinary concentration of androgens, AAS metabolites, and fluoxetine were monitored through urine samples analysis at the Brazilian Laboratory of Doping Control.
DRUG TESTING AND ANALYSIS
(2021)
Article
Medicine, Legal
Vinicius L. Meira, Adriana S. de Oliveira, Luciana S. A. Cohen, Cecilia de A. Bhering, Karen M. de Oliveira, Denilson S. de Siqueira, Marco Antonio Martins de Oliveira, Francisco R. de Aquino Neto, Gabriela Vanini
Summary: This study analyzed drugs of abuse from blotter papers seized in Rio de Janeiro between 2006 and 2019 using advanced analytical techniques. It found that synthetic phenethylamines, such as 25I-NBOH and 251-NBOMe, were the most common NPS compounds. Prior to 2014, LSD was the main substance found in seized blotter papers, while an increase in NBOMe compounds was observed from 2014 to 2017, with NBOH compounds emerging in coastal regions from 2016.
FORENSIC SCIENCE INTERNATIONAL
(2021)
Article
Chemistry, Analytical
Rebecca Rodrigues Matos, Carina de Souza Anselmo, Vinicius Figueiredo Sardela, Henrique Macelo Gualberto Pereira
Summary: Stanozolol metabolism was investigated in Zebrafish Water Tank, producing hydroxylated-sulfate, hydroxylated-glycoconjugate, and glucuronide metabolites. STAN-O-Glucuronide was the most intense phase II metabolite, requiring high concentrations of fish and substrate for formation.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Biochemical Research Methods
Julio X. Amaral, Andrea C. Deslandes, Monica C. Padilha, Leonardo Vieira Neto, Luiz E. Osorio, Francisco Radler Aquino Neto, Marcelo S. Cruz
Summary: The use of androgenic-anabolic steroids (AAS) can lead to psychiatric symptoms such as insomnia, anxiety, and increased aggressiveness. This study on bodybuilders found that the majority of participants experienced symptoms of agitation, insomnia, increased aggressiveness, or depression. There was no significant difference in psychiatric symptoms between males and females, and no direct association between symptoms and drug dosage.
DRUG TESTING AND ANALYSIS
(2022)
Letter
Infectious Diseases
A. J. C. Rodrigues, F. de Oliveira Barros, K. G. Pereira Martins, T. H. Pacheco Almeida, J. da Silva Matsuda, N. H. Mallmann, C. de Souza Anselmo, V. F. Sardela, J. P. de Moura Neto, I. R. dos Santos Magalhaes
INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Jessica Correa Bezerra Bellei, Nicolas Glanzmann, Barbara Albuquerque Carpinter, Daniela Chaves Renhe, Carolina Brandi Marques, Marina Rocha Azevedo, Livia Maria Barreto, Vinicius Novaes Rocha, Isabelle Karine da Costa Nunes, Henrique Marcelo Gualberto Pereira, Elaine Soares Coimbra, Eduardo Antonio Ferraz Coelho, Adilson David da Silva, Fernando de Pilla Varotti, Kezia Katiani Gorza Scopel
Summary: Chloroquine remains the most effective drug for malaria treatment, although resistance to it has emerged. This study focuses on the synthesis of quinoline derivatives to develop new antimalarials. Compound 3 showed promising activity against malaria, inhibiting parasite growth and protecting against severe malaria development.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Article
Immunology
Carolinne Souza Amorim, Joao Alfredo Moraes, Ingrid de Jesus Magdalena, Sheila Gutierrez Lopez, Ana Carolina Dudenhoeffer Carneiro, Isabelle Karine da Costa Nunes, Luciana Pizzatti, Vinicius Figueiredo Sardela, Francisco Radler Aquino Neto, Luciana Cristina Mirotti, Henrique Marcelo Gualberto Pereira, Mariana Renovato-Martins
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Plant Sciences
Fabio Junior Moreira Novaes, Maria Alice Esteves da Silva, Diana Cardoso Silva, Francisco Radler de Aquino Neto, Claudia Moraes Rezende
Summary: This article describes the extraction of cafestol and kahweol, two significant furane-diterpenoids from coffee beans, and investigates their formation and degradation kinetics during the roasting process. It also explains how the degree of roasting and the preparation method of the coffee beverage influence the content of these compounds.
Article
Biochemical Research Methods
Christiane Martins de Vasconcellos Silveira, Vanessa Farelo dos Santos, Isis Moraes Ornelas, Beatriz de Sa Carrilho, Matheus Antonio Vieira de Castro Ventura, Henrique Marcelo Gualberto Pereira, Stevens Kastrup Rehen, Magno Junqueira
Summary: This study systematically characterized LSD metabolites in Caenorhabditis elegans, revealing their metabolic pathways and providing a foundation for further research on the therapeutic potential of LSD.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Review
Plant Sciences
Fabio Junior Moreira Novaes, Daliane Claudia de Faria, Fabio Zamboni Ferraz, Francisco Radler de Aquino Neto
Summary: In many analytical chemical procedures, organic solvents are used to separate, extract, or isolate target phytochemical analytes. The selection of extraction solvents is based on solubility differences, cost, and safety. Hansen Solubility Parameters are used to predict the miscibility of compounds, saving time and costs in method development. This review discusses how Hansen Solubility Parameters have been incorporated into classical and emerging extraction techniques, saving resources and avoiding unnecessary solvent wasting.
Article
Chemistry, Multidisciplinary
Clarisse L. Torres, Vinicius F. Sardela, Fernanda B. Scalco, Francisco R. de Aquino Neto, Rafael Garrett
Summary: Metabolic profiling using LC-MS allows detection of metabolites with distinct concentrations and properties. Quality control strategies are necessary for obtaining high-quality data. A test mixture (TM) was developed and used for untargeted metabolomics of urine samples, providing a rapid assessment of LC-MS system performance and avoiding analysis of poor-quality data.
