Article
Biochemistry & Molecular Biology
Michael J. Haas, Shrina Parekh, Poonam Kalidas, Angela Richter, Firas Warda, Norman C. W. Wong, Masaaki Tokuda, Arshag D. Mooradian
Summary: This study demonstrates that rare sugars D-allulose and D-tagatose can increase the production of apo A-I by enhancing the binding of transcription factor SP1 to the promoter. These sugars modulate insulin responsive genes and may have anti-atherogenic properties.
JOURNAL OF FOOD BIOCHEMISTRY
(2022)
Article
Pharmacology & Pharmacy
Yansong Guo, Wei Li, Mingming Qian, Ting Jiang, Ping Guo, Qian Du, Na Lin, Xianwei Xie, Zhiyong Wu, Donghai Lin, Donghui Liu
Summary: D-4F alleviates endothelial oxidative injuries caused by iodinated contrast media by upregulating HO-1 expression and suppressing ROS production, potentially serving as a promising agent in preventing CI-AKI.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Chemistry, Analytical
Ainan Zhou, Jinghua Yu, Yali Wu, Hao Xue, Dafang Zhong, Xingxing Diao
Summary: We aimed to establish an LC-MS/MS method for quantifying bevacizumab content in biological fluids using a surrogate peptide generated through trypsin hydrolysis and an isotopically labeled peptide as an internal standard. The method involved a series of pre-treatment processes and had a total duration of approximately 2.5-3 h. The validated method showed a linear range of 0.2 μg/mL to 300 μg/mL in different matrices, with satisfactory precision and accuracy.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Immunology
Myriam Fernandez-Sendin, Claudia Augusta Di Trani, Angela Bella, Marcos Vasquez, Nuria Ardaiz, Celia Gomar, Leire Arrizabalaga, Sergio Ciordia, Fernando J. Corrales, Fernando Aranda, Pedro Berraondo
Summary: Apolipoprotein A-I mimetic peptide L37pA modulates the gene expression program of interferon alpha, reducing inflammatory pathways activation and inducing an anti-inflammatory effect in the liver. This modulation allows for the silencing of interferon alpha expression mediated by an adeno-associated virus, preventing lethal hematological toxicity in mice. The proteomic analysis confirms the impact of L37pA on several inflammatory pathways and indicates the activation of LXR/RXR and PPPAR alpha/gamma nuclear receptors.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Barbara Prandi, Chiara Vacca, Stefano Sforza, Tullia Tedeschi
Summary: The increasing awareness of consumers on sustainable food chains has caused a shift in consumption from animal protein sources to plant protein sources. Soy, which is both relevant for human food use and animal feed, has a high protein content but is accompanied by antinutritional factors such as Kunitz's trypsin inhibitor (KTI). A direct label-free liquid chromatography-mass spectrometry (LC-MS) method has been developed to identify and quantify Kunitz inhibitor KTI3 in soybean and its derivative products, using a marker peptide specific to the protein of interest. The results of the LC-MS method were compared with spectrophotometric trypsin inhibition, demonstrating the complementarity of these two types of analysis.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Marie Bicikova, Ludmila Macova, Martin Hill
Summary: Vitamin D plays an important role in immunomodulation, brain development, and functioning. Its potential immunoprotective role in COVID-19 has been discussed. This study found that individuals who took vitamin D had a significantly lower risk of COVID-19 infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemical Research Methods
Fayaj A. Mulani, Sharvani S. Nandikol, Jagadeesh S. Kajjihundi, Niranjana Pathappa, Sharanappa Puttappa, Hirekodathakallu Thulasiram
Summary: Meliaceae plants are widely used in agriculture, folklore, and traditional medicine, and contain diverse limonoids with various bioactivities. Accurate detection of these compounds from plant tissue extracts or biological samples is challenging. In this study, a new method was developed for the identification, quantification, and characterization of limonoids in different plant tissue extracts using minimal plant material.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Jinxin Li, Zhenyu Zhu, Yiteng Li, Yihao Chen, Xuyang Hu, Yanchang Liu, Yi Shi, Yao Hu, Yihui Bi, Xinzhong Xu, Meige Zheng, Li Cheng, Juehua Jing
Summary: We found that D-4F can promote the clearance of myelin debris and reduce the formation of foamy macrophages, leading to neuroprotection and motor function recovery. These beneficial effects may be associated with the upregulation of ABCA1 expression by D-4F.
Article
Medical Laboratory Technology
Lisa E. Kilpatrick, Roger Bouillon, W. Clay Davis, Clark M. Henderson, Andrew N. Hoofnagle, Steven Pauwels, Dirk Vanderschueren, Etienne Waelkens, Hans Wildiers, James H. Yen, Karen W. Phinney
Summary: This study investigated the influence of different proteoforms of vitamin D-binding protein (VDBP) on quantification using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The results showed that VDBP proteoforms do not cause bias and a family of proteins can be accurately quantified using shared peptides. Assigning a reference value in a standard reference material can improve the accuracy of VDBP quantification in research and clinical samples.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Biochemical Research Methods
Xuan Guo, Lei Zhang, Zihan Lei, Zhe Hou, Hui Li, Xiaodong Li, Jing Dong, Ling Song, Dingding Chen, Dongyang Liu
Summary: The objective of this study was to develop a LC-MS/MS method for quantitation of seven cytochrome P450 (CYP450) enzymes. The method was validated for selectivity, linearity, stability, accuracy, and precision. The abundance of the enzymes in human liver microsomes and CYP3A4 in placenta was accurately determined using this method.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Biochemical Research Methods
Rongmei Huang, Yi Hong, Yike Wu, Weifeng Li, Wenlan Liu
Summary: We developed a new LC-MS/MS method for simultaneous determination of serum testosterone and free testosterone levels. The method is simple to perform and can be applied in clinical practice, providing important information for diagnosis, treatment, and monitoring of androgen-related diseases.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Tengfei Yuan
Summary: In this study, a sample-multiplexing LC-MS/MS method was developed and validated for the quantification of serum estrogens. The method demonstrated high accuracy and precision, and allowed simultaneous analysis of E1, E2, and E3.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Pharmacology & Pharmacy
Kevin McKinski, Dean McNulty, Francesca Zappacosta, Mary Birchler, Matt Szapacs, Christopher Evans
Summary: ICOS, expressed on the surface of activated T-cells, is being investigated as a potential therapeutic target. Recent studies found aberrant increases in sICOS in disease-state patients using commercial ELISA kits, but the results were not consistent with in-house immunoassays. The researchers developed a novel LC-MS/MS method to confirm the results and demonstrated that sICOS is a low abundance soluble protein.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2022)
Article
Biochemical Research Methods
Hyojin Kim, Dong Huey Cheon, Won Suk Yang, Je-Hyun Baek
Summary: This study developed a protein-MRM method without digestion to individually quantify APOC-III proteoforms in human serum. The method provides an affordable and rapid quantification of potential disease-specific proteoforms and allows investigators to identify novel biological roles potentially related to cardiovascular disease or novel biomarkers.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Chemistry, Medicinal
Yodying Yingchutrakul, Sucheewin Krobthong, Kiattawee Choowongkomon, Phakorn Papan, Pawitrabhorn Samutrtai, Thanisorn Mahatnirunkul, Thitikorn Chomtong, Nitipol Srimongkolpithak, Theeranuch Jaroenchuensiri, Chanat Aonbangkhen
Summary: This study identified and investigated the effects of a novel multifunctional peptide from Lingzhi on melanogenic enzymes in melanoma cells through a targeted-proteomics approach. The synthesized octapeptide exhibited strong antioxidant activity and regulated the expression of specific proteins involved in pigmentation pathways. These findings contribute to the understanding of the molecular functions of the multifunctional octapeptide on melanogenic enzymes and support its potential as a therapeutic and cosmetic ingredient.
