Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 877, Issue 1-2, Pages 89-94Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2008.11.012
Keywords
Fat-soluble vitamins; Vitamin A; Vitamin E; CoQ(10); UHPLC; Serum; UV
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We are presenting the first ultra-high pressure LC (UHPLC) method for rapid quantitative measurement of vitamin A, E (alpha- and gamma-tocopherol), beta-carotene and CoQ(10) from human serum. The chromatography was performed on Shield RP(18) UHPLC Column with UV detection. The method was validated based on linearity, accuracy. matrix effects study, precision and stability. The calibration was linear over the following range: 0.09-10.0 for retinol and gamma-tocopherol, 0.05-5 for beta-carotene, 0.9-100 for alpha-tocopherol and 0.14-15 mg/L for CoQ(10). The limit of detection and quantitation for retinol, gamma-tocopherol, P-carotene, alpha-tocopherol and CoQ10 were as follows 0.07/0.024, 0.018/0.06. 0.004/0.12, 0.078/0.261, 0.008/0.028 mg/L The recoveries were above 85%. The inter- and intra-assay precision was below 10%. Reference intervals were established for children and adults. Because of its low cost, extremely short analysis time (2 min) and excellent chromatographic reproducibility this UHPLC method can easily be adopted for high-throughput clinical and pharmacokinetics Studies. (C) 2008 Elsevier B.V. All rights reserved.
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